FastQCFastQC Report
Thu 26 May 2016
SRR1364329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364329_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528240
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT235441.5405957179500602No Hit
GTACTTTTTTTTTTTTTTTTTTTTT176161.1526985290268543No Hit
TATCAACGCAGAGTACTTTTTTTTT174191.1398078835784955No Hit
GGTATCAACGCAGAGTACTTTTTTT166711.0908626917238131No Hit
GAGTACTTTTTTTTTTTTTTTTTTT87070.5697403549180756No Hit
ACGCAGAGTACTTTTTTTTTTTTTT71420.46733497356436166No Hit
GTGGTATCAACGCAGAGTACTTTTT35260.23072292310108358No Hit
ATCAACGCAGAGTACTTTTTTTTTT28100.18387164319740357No Hit
GTGGTATCAACGCAGAGTACATGGG25190.16483013139297492No Hit
GCAGAGTACTTTTTTTTTTTTTTTT23850.15606187509815211No Hit
GTATAACGCAGAGTACTTTTTTTTT21760.1423860126681673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18920.12380254410302048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTTCG307.7333924E-418.99607314
TTCGACC250.0060403818.99545117
TCGACCG350.002174125816.28128218
ATAACGC2850.014.6679563
CGACCGT400.00526795514.254518519
GGTATCA39050.014.0140361
GCCCCCG1301.8189894E-1213.880836518
TATAACG3150.013.8724182
CGCGGTT551.9646484E-413.81442218
GTGCACG1351.8189894E-1213.36979511
TAACGCA3500.013.3016044
ACGGCCC1301.4551915E-1113.15112715
GTGGTAT10100.012.6991361
TATCACG1301.9826984E-1012.4225412
CCCCCGC1405.2750693E-1112.2181619
GTGTAAG1405.2750693E-1112.215361
TGGTATC10450.011.8176732
AGGACCG1052.7083843E-711.7656355
ATTAACG658.017373E-411.6933363
CGGCCCC1552.5465852E-1111.64275516
Produced by FastQC (version 0.11.5)