##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364329_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1528240 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.45691841595561 31.0 30.0 34.0 26.0 34.0 2 29.472266790556457 31.0 30.0 34.0 25.0 34.0 3 29.504524158509135 31.0 30.0 34.0 25.0 34.0 4 32.82018727425012 35.0 33.0 37.0 26.0 37.0 5 32.40306365492331 35.0 32.0 37.0 25.0 37.0 6 32.2716412343611 35.0 32.0 37.0 23.0 37.0 7 31.993436240381094 35.0 32.0 37.0 19.0 37.0 8 31.869822148353663 35.0 32.0 37.0 17.0 37.0 9 32.57391640056536 37.0 32.0 39.0 11.0 39.0 10 32.70895605402293 37.0 32.0 39.0 17.0 39.0 11 32.62522705857719 37.0 32.0 39.0 13.0 39.0 12 32.57760430298906 37.0 32.0 39.0 15.0 39.0 13 32.59543069151442 37.0 32.0 39.0 13.0 39.0 14 33.48299808930535 38.0 32.0 41.0 11.0 41.0 15 33.51262890645448 38.0 32.0 41.0 11.0 41.0 16 33.47985394964142 38.0 32.0 41.0 11.0 41.0 17 33.49550070669528 38.0 32.0 41.0 11.0 41.0 18 33.460499005391824 38.0 32.0 41.0 10.0 41.0 19 33.39177485211747 39.0 32.0 41.0 10.0 41.0 20 33.294138355232164 39.0 32.0 41.0 8.0 41.0 21 33.23810396272837 38.0 32.0 41.0 2.0 41.0 22 33.16976522012249 38.0 32.0 41.0 2.0 41.0 23 32.9015298644192 38.0 32.0 41.0 2.0 41.0 24 32.81988496571219 38.0 32.0 41.0 2.0 41.0 25 32.65354460032456 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5579421045312074 1101 2 0.8021159285831345 1101 3 -1.2710703468936444 1101 4 -0.13278040448002315 1101 5 0.5943368701565674 1101 6 0.9402954217886119 1101 7 1.1400053774668706 1101 8 1.3332146004434726 1101 9 1.839304049150229 1101 10 1.5350963004464688 1101 11 1.5564628513021859 1101 12 1.5272628385202225 1101 13 1.2746871861452718 1101 14 1.5546606128107143 1101 15 1.5021165444908533 1101 16 1.4829339947543616 1101 17 1.4153837686279829 1101 18 1.3832354911265483 1101 19 1.4426344222513308 1101 20 1.7175252655056426 1101 21 1.5882374110675812 1101 22 1.6016867330298439 1101 23 1.9209879309351976 1101 24 1.8300571372175796 1101 25 1.9565479613551062 1102 1 0.7281405196432509 1102 2 0.6616725393790261 1102 3 0.8061708561382623 1102 4 0.6515381979685557 1102 5 0.9214412201990001 1102 6 1.064851554685177 1102 7 0.9760591165222507 1102 8 1.0501074003832294 1102 9 1.6389661163979028 1102 10 1.0514524049587024 1102 11 1.2039733578465146 1102 12 1.375614520291407 1102 13 1.2452167275646602 1102 14 1.2184435914848137 1102 15 1.2388110306911457 1102 16 1.2778587038874534 1102 17 1.2605422139780273 1102 18 1.2770600180786218 1102 19 1.3147865767083928 1102 20 1.5424204172893639 1102 21 1.5865321793733003 1102 22 1.5225781021679765 1102 23 1.867143158429755 1102 24 1.8027288985807246 1102 25 1.5328728286294293 1103 1 -2.2221191404847787 1103 2 -2.0647031022728584 1103 3 -2.155212198253249 1103 4 -2.145869057089673 1103 5 -4.131645564557324 1103 6 -8.613204165121836 1103 7 -8.768486306860872 1103 8 -10.812673362486233 1103 9 -11.715641351187426 1103 10 -8.715913972911054 1103 11 -10.528045392528409 1103 12 -9.975125896707333 1103 13 -11.519396653073095 1103 14 -11.100728409783489 1103 15 -10.69715875275402 1103 16 -5.206323880887723 1103 17 -3.333044695038094 1103 18 -2.5661969597517142 1103 19 -1.915321143681357 1103 20 -7.387565614195498 1103 21 -3.9945152021728347 1103 22 -9.07919709900435 1103 23 -10.372395452088622 1103 24 -11.80896994708861 1103 25 -12.105415471239457 1104 1 0.24724268220239765 1104 2 0.4596788907415288 1104 3 0.5228427394597652 1104 4 0.6722237687012793 1104 5 0.6759307824568737 1104 6 0.6331317701978065 1104 7 0.4622271391782178 1104 8 0.7270689478408023 1104 9 1.1387601778671481 1104 10 0.9862861573292179 1104 11 0.8580112873596164 1104 12 0.9512058946037811 1104 13 0.8495300487189823 1104 14 1.0109018769988154 1104 15 0.9379549537656047 1104 16 0.5251803096351821 1104 17 0.47654445611673424 1104 18 0.6105788324727754 1104 19 0.9348729481464915 1104 20 1.1301775080189245 1104 21 0.8902263892955844 1104 22 0.7114353274394958 1104 23 1.2103451752635266 1104 24 1.3860838827364148 1104 25 1.4940322761959095 1105 1 0.47642566188453017 1105 2 0.4478816865476105 1105 3 0.20160217394036195 1105 4 0.42052243493301233 1105 5 0.43515412972524103 1105 6 0.5355899011806358 1105 7 0.6048385439716029 1105 8 0.5996304615872781 1105 9 0.7908204037348838 1105 10 0.30329541747795474 1105 11 0.8499108169227796 1105 12 0.8906558974718735 1105 13 0.7703574702039901 1105 14 0.5137202131852661 1105 15 0.34642986621956595 1105 16 0.5356640641178814 1105 17 0.5690453873840227 1105 18 0.6156788599097425 1105 19 0.6067956021649721 1105 20 0.9691467074331399 1105 21 0.8247771314413157 1105 22 0.6778291909582137 1105 23 1.0340654343182436 1105 24 0.92689930659607 1105 25 0.9337545753998242 1106 1 0.2875963701554518 1106 2 0.5776475689334113 1106 3 0.352781494181567 1106 4 -1.0744233354618196 1106 5 0.060522109387413536 1106 6 0.43832059574876325 1106 7 0.7559533451414993 1106 8 0.9418150455241125 1106 9 1.4591448275442218 1106 10 1.129441444110718 1106 11 0.9755648016736487 1106 12 1.304646849246609 1106 13 1.0996640475079147 1106 14 0.49141482126461256 1106 15 1.1025490140304655 1106 16 0.7564136802735959 1106 17 0.8758890755274678 1106 18 0.8935133594768416 1106 19 -0.4164178293976164 1106 20 0.8013260626737377 1106 21 0.5325515204457147 1106 22 1.118360990729201 1106 23 1.0297702318149717 1106 24 1.066510799263341 1106 25 1.4078983366177482 1107 1 -0.0713161019606332 1107 2 -0.25690277671670003 1107 3 -0.3742452964626892 1107 4 -0.26249303972667803 1107 5 -0.28431946580528944 1107 6 -0.253733736472892 1107 7 -0.3296593942184387 1107 8 -0.5962794395345234 1107 9 0.29817091142787433 1107 10 -2.0371789206308222E-4 1107 11 -0.07315662421587632 1107 12 -0.12625449138991485 1107 13 -0.3185436959379899 1107 14 0.10538553487196367 1107 15 -0.1755429090209759 1107 16 -0.25333961610204625 1107 17 -0.34968169992144027 1107 18 -0.2929489944777046 1107 19 -0.12917247303566626 1107 20 -0.06116518012061789 1107 21 0.017695324142955826 1107 22 0.04853268938414601 1107 23 0.18455734226840548 1107 24 0.483376227626529 1107 25 0.3336556085218021 1108 1 0.022168962050745478 1108 2 0.06912291815110194 1108 3 0.13786254069777826 1108 4 0.03182889122683008 1108 5 0.3391613985176818 1108 6 0.16693974904269737 1108 7 0.048254218642540536 1108 8 0.4007023843946662 1108 9 0.7238231501327448 1108 10 0.49284891368688477 1108 11 0.5591611070492277 1108 12 0.49167411390710924 1108 13 -0.09063283771133968 1108 14 0.1512916515149243 1108 15 -0.05620004736232431 1108 16 -0.5213926315234829 1108 17 -0.562227117519484 1108 18 -0.5758185106103753 1108 19 -0.4340711160395756 1108 20 0.2233730122070483 1108 21 -0.1154933231509574 1108 22 0.13724799115207986 1108 23 -0.27652491291911474 1108 24 -0.004977810622243339 1108 25 -0.1378425964500849 1109 1 0.22747084094675785 1109 2 0.327242721503179 1109 3 0.22074668226162686 1109 4 0.25632513039299454 1109 5 0.3259515417870418 1109 6 0.36179562482767125 1109 7 0.5096436555821029 1109 8 0.554039095188589 1109 9 1.1402892145205712 1109 10 0.6273232413152456 1109 11 0.546400704313271 1109 12 0.5923780312369402 1109 13 0.7633397167502096 1109 14 0.3875633755617045 1109 15 0.1174863879378023 1109 16 0.4734394342132262 1109 17 0.3803140964153684 1109 18 0.19013186169696183 1109 19 0.3729185491407989 1109 20 0.6687153157400587 1109 21 0.701491317794563 1109 22 0.3064619134222397 1109 23 0.6349059031571826 1109 24 0.42429743038893974 1109 25 0.48803179348298187 1110 1 0.06799739918353254 1110 2 0.11383466420376465 1110 3 0.09982095816208769 1110 4 0.1685628853358736 1110 5 0.10592549238457849 1110 6 0.1715116280558604 1110 7 0.18036212035629262 1110 8 0.21081155898982473 1110 9 -3.1907729924195145 1110 10 -1.2194631545780474 1110 11 -0.24834599952330194 1110 12 0.13725927066658272 1110 13 -0.11026156843312407 1110 14 -0.015395149060545066 1110 15 0.12878472843919297 1110 16 -0.24405370322966036 1110 17 -0.03761223735808272 1110 18 0.006889582053020149 1110 19 -0.09836633945710815 1110 20 0.20393184932825648 1110 21 0.16720767470648212 1110 22 0.28392848484597977 1110 23 0.43202987162104023 1110 24 0.4868520932352922 1110 25 0.42971189715073166 1111 1 0.05092562953573676 1111 2 0.08891025296733801 1111 3 0.0906972912203905 1111 4 0.030859300661866484 1111 5 -0.004560936534133475 1111 6 0.1293575756223717 1111 7 0.3278918248697451 1111 8 0.2669596408253341 1111 9 0.9299442175254882 1111 10 0.3739389219445215 1111 11 0.3876117707600031 1111 12 0.6558151335394555 1111 13 0.41621505063943687 1111 14 0.1511465848958693 1111 15 0.4839623280601657 1111 16 0.4756229637580063 1111 17 0.4984888420404019 1111 18 0.22691940484430262 1111 19 0.4386037884272369 1111 20 0.7211883337217913 1111 21 0.5694814947718356 1111 22 0.4978947650936547 1111 23 0.7991676252011715 1111 24 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-0.4702864033811238 2215 3 -0.22783995349633557 2215 4 -0.4874389799963481 2215 5 -0.48462727443300224 2215 6 -0.5693424229936639 2215 7 -0.593925870464922 2215 8 -0.7901357002981655 2215 9 0.1293615696376449 2215 10 0.010406774820161502 2215 11 0.12579158429422677 2215 12 0.22967947761079444 2215 13 0.09616928763826849 2215 14 -0.1706060642375462 2215 15 -0.4290948895309086 2215 16 -0.3412927391330882 2215 17 -0.5103408337896269 2215 18 -0.3315806574339746 2215 19 -0.29423854603940924 2215 20 -0.17801081375778693 2215 21 -0.1724880672965412 2215 22 -0.3151689109854914 2215 23 0.06008823654320139 2215 24 0.24913184572204727 2215 25 0.13366434544791872 2216 1 -0.7793723812727755 2216 2 -0.6777928236161266 2216 3 -0.7775261719186553 2216 4 -0.5443981986636217 2216 5 -1.3514669384472882 2216 6 -1.8280495259465752 2216 7 -1.406378518762473 2216 8 -2.100642128434991 2216 9 -0.9622188749191807 2216 10 -1.2940817793824237 2216 11 -0.849123273512383 2216 12 -1.9705275502747526 2216 13 -1.8514962754064612 2216 14 -1.9096556324026608 2216 15 -1.4735647954201418 2216 16 -2.4451061611776055 2216 17 -2.843034487831808 2216 18 -2.4376535280889584 2216 19 -3.7609473557871027 2216 20 -2.894853559484872 2216 21 -3.1267924298956054 2216 22 -2.8261145227054847 2216 23 -2.2684452632553054 2216 24 -2.392888493582433 2216 25 -2.321093740097268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61424.0 3 1.0 4 826.0 5 8642.0 6 11973.0 7 9465.0 8 7212.0 9 5883.0 10 4991.0 11 4561.0 12 4057.0 13 3721.0 14 3664.0 15 3641.0 16 3842.0 17 4101.0 18 4546.0 19 5892.0 20 8040.0 21 10408.0 22 12628.0 23 14652.0 24 16775.0 25 19155.0 26 20991.0 27 22614.0 28 24929.0 29 29356.0 30 35326.0 31 44050.0 32 53811.0 33 67132.0 34 82975.0 35 105044.0 36 138667.0 37 207534.0 38 465711.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41964519473447 19.21432049764894 13.532906931688466 22.833127375928118 2 15.358748135899546 21.842971253428015 40.021476007661214 22.77680460301122 3 18.292658987224485 28.48997144420103 29.846306460520495 23.371063108053992 4 11.643907329318058 18.142948008198072 37.23618669200427 32.97695797047961 5 11.801789064269379 39.48319266674783 34.353738824893995 14.361279444088796 6 28.162338962686068 38.855229696177254 18.63530406488742 14.34712727624926 7 25.656998273485637 34.11299179153026 22.489322182764436 17.740687752219664 8 25.759412393819716 35.2652723543126 20.299955623218874 18.675359628648813 9 26.233193102667308 15.972942703543383 20.561673237456315 37.23219095633299 10 15.246848120655121 31.337863137085474 31.636358875666055 21.77892986659335 11 32.500109643309045 24.40725517958265 23.586206806263874 19.506428370844436 12 22.878969621179255 28.262125712550638 30.155149701891908 18.703754964378195 13 28.092809477256335 23.015090897431367 27.170551614114853 21.721548011197445 14 21.53161365386843 22.416996203914135 29.933717369923034 26.117672772294398 15 22.953091810011426 29.264645639714843 26.51953297289374 21.262729577379993 16 20.968934620831185 29.562171330748914 27.526745071760818 21.942148976659084 17 20.550408644454784 28.527499163995053 29.03283225388898 21.889259937661187 18 19.78498930312948 26.53857908648922 32.54898342099597 21.12744818938533 19 21.29341792332189 26.229615483789896 32.74690150117998 19.730065091708234 20 21.498040278189528 25.355181172011154 32.9508783571975 20.195900192601812 21 22.2860646817188 24.88987721088146 32.695621330740074 20.12843677665966 22 21.604949986420912 25.161231214232195 32.70204209844348 20.531776700903418 23 20.923149813234073 25.17956751974327 33.289095853979674 20.608186813042987 24 20.791558938297246 25.419837987002573 33.081001918006685 20.70760115669349 25 20.722533583820027 25.196642535051105 33.16715008502399 20.913673796104877 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2717.0 1 2717.0 2 3301.5 3 3886.0 4 3886.0 5 3886.0 6 12407.5 7 20929.0 8 20929.0 9 20929.0 10 17941.0 11 14953.0 12 14953.0 13 14953.0 14 13847.0 15 12741.0 16 12741.0 17 12741.0 18 19859.0 19 26977.0 20 26977.0 21 26977.0 22 39528.5 23 52080.0 24 52080.0 25 52080.0 26 73334.0 27 94588.0 28 94588.0 29 94588.0 30 108390.0 31 122192.0 32 122192.0 33 122192.0 34 131126.0 35 140060.0 36 140060.0 37 140060.0 38 144704.0 39 149348.0 40 149348.0 41 149348.0 42 157668.5 43 165989.0 44 165989.0 45 165989.0 46 174859.0 47 183729.0 48 183729.0 49 183729.0 50 178335.0 51 172941.0 52 172941.0 53 172941.0 54 155893.5 55 138846.0 56 138846.0 57 138846.0 58 119189.0 59 99532.0 60 99532.0 61 99532.0 62 81393.5 63 63255.0 64 63255.0 65 63255.0 66 48604.0 67 33953.0 68 33953.0 69 33953.0 70 25425.0 71 16897.0 72 16897.0 73 16897.0 74 11997.5 75 7098.0 76 7098.0 77 7098.0 78 5308.0 79 3518.0 80 3518.0 81 3518.0 82 2401.5 83 1285.0 84 1285.0 85 1285.0 86 879.0 87 473.0 88 473.0 89 473.0 90 353.0 91 233.0 92 233.0 93 233.0 94 125.5 95 18.0 96 18.0 97 18.0 98 10.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02722085536303198 2 0.0015049992147830183 3 2.6173899387530754E-4 4 0.004973040883630844 5 0.03363346071297702 6 0.011647385227451184 7 0.020023033031461027 8 0.02708998586609433 9 0.03396063445532115 10 0.037952154111919593 11 0.03657802439407423 12 0.006739779092289169 13 0.002224781447940114 14 0.005692823116787939 15 0.00693608333769565 16 0.007066952834633303 17 0.009095430037166938 18 0.014591948908548397 19 0.015835209129456105 20 0.014199340417735436 21 0.011320211485107052 22 0.010142386012668168 23 0.008113908810134534 24 0.005954562110663247 25 0.10548081453174893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1528240.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.22939737324013 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49837313708248 37.167394121985915 2 12.87556718848229 12.677128270552936 3 4.757331703174157 7.026017185556216 4 2.1729255725105863 4.278872658863959 5 1.2600153017821045 3.101489699389716 6 0.7495500086110757 2.2139937135018126 7 0.49937806987862376 1.720885700907551 8 0.34784363289846254 1.36993059421679 9 0.26034829683054855 1.1535110784105616 >10 1.4043466242018503 12.759113848537975 >50 0.11544455116989749 3.9038225377973883 >100 0.053958582603512266 4.436339660697133 >500 0.0026580166348349602 0.852962393723431 >1k 0.001461909149159228 1.3782699843658661 >5k 2.65801663483496E-4 1.0369410890636386 >10k+ 5.31603326966992E-4 4.9233274624291 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 23544 1.5405957179500602 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 17616 1.1526985290268543 No Hit TATCAACGCAGAGTACTTTTTTTTT 17419 1.1398078835784955 No Hit GGTATCAACGCAGAGTACTTTTTTT 16671 1.0908626917238131 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 8707 0.5697403549180756 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 7142 0.46733497356436166 No Hit GTGGTATCAACGCAGAGTACTTTTT 3526 0.23072292310108358 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2810 0.18387164319740357 No Hit GTGGTATCAACGCAGAGTACATGGG 2519 0.16483013139297492 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 2385 0.15606187509815211 No Hit GTATAACGCAGAGTACTTTTTTTTT 2176 0.1423860126681673 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1892 0.12380254410302048 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 6.543474846882689E-5 0.0 12 0.0 0.0 0.0 6.543474846882689E-5 0.0 13 0.0 0.0 0.0 1.3086949693765377E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGTTCG 30 7.7333924E-4 18.996073 14 TTCGACC 25 0.00604038 18.995451 17 TCGACCG 35 0.0021741258 16.281282 18 ATAACGC 285 0.0 14.667956 3 CGACCGT 40 0.005267955 14.2545185 19 GGTATCA 3905 0.0 14.014036 1 GCCCCCG 130 1.8189894E-12 13.8808365 18 TATAACG 315 0.0 13.872418 2 CGCGGTT 55 1.9646484E-4 13.814422 18 GTGCACG 135 1.8189894E-12 13.369795 11 TAACGCA 350 0.0 13.301604 4 ACGGCCC 130 1.4551915E-11 13.151127 15 GTGGTAT 1010 0.0 12.699136 1 TATCACG 130 1.9826984E-10 12.422541 2 CCCCCGC 140 5.2750693E-11 12.21816 19 GTGTAAG 140 5.2750693E-11 12.21536 1 TGGTATC 1045 0.0 11.817673 2 AGGACCG 105 2.7083843E-7 11.765635 5 ATTAACG 65 8.017373E-4 11.693336 3 CGGCCCC 155 2.5465852E-11 11.642755 16 >>END_MODULE