FastQCFastQC Report
Thu 26 May 2016
SRR1364328_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364328_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494130
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT192600.772213156491442No Hit
TATCAACGCAGAGTACTTTTTTTTT110980.44496477729709355No Hit
GGTATCAACGCAGAGTACTTTTTTT87150.349420439191221No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN69400.27825333883959535No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA54480.21843288040318667No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45160.18106514095095283No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41010.16442607241803756No Hit
GTGGTATCAACGCAGAGTACATGGG35320.14161250616447418No Hit
GAGTACTTTTTTTTTTTTTTTTTTT31540.12645692085015617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG350.002180344416.2748992
CGCATAT1700.016.2075148
GGTATCA39550.013.8747511
CTAGACG500.001507286313.2914363
TCATCGC1403.6379788E-1212.900914
TTCGCGG751.469044E-512.67534616
GTTATCA6500.012.5611361
TCGCGGT701.0844512E-412.22265417
GCGTATC701.0979896E-412.206421
TAACGCA1650.012.0984624
TGGTATC10550.012.0583382
CTCAACG1353.765308E-1011.9552593
ACTGCGT658.0196117E-411.6934858
TTGACCG1552.5465852E-1111.652904518
GTGGTAT11100.011.4610831
CGAAAGC752.0616098E-411.40781119
AAATTCG752.0627677E-411.40712114
CATCGCA1354.6875357E-911.2662935
TCAACTC11950.011.2162075
CGCAGAT9600.011.1834178
Produced by FastQC (version 0.11.5)