##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364328_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2494130
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.204775613139653	30.0	26.0	31.0	7.0	34.0
2	25.92986853131152	30.0	25.0	31.0	7.0	34.0
3	25.478755718426868	30.0	25.0	31.0	7.0	34.0
4	29.174743497732678	35.0	28.0	37.0	7.0	37.0
5	29.82911716710837	35.0	30.0	37.0	7.0	37.0
6	29.946016847558067	35.0	30.0	37.0	2.0	37.0
7	29.522637953915794	35.0	30.0	37.0	2.0	37.0
8	29.084105078724846	35.0	28.0	37.0	2.0	37.0
9	30.078208433401628	35.0	28.0	39.0	2.0	39.0
10	29.39837538540493	35.0	27.0	39.0	2.0	39.0
11	30.045710929261908	35.0	28.0	39.0	2.0	39.0
12	29.57094938916576	35.0	27.0	39.0	2.0	39.0
13	29.12412103619298	35.0	27.0	39.0	2.0	39.0
14	29.43130069402958	36.0	25.0	40.0	2.0	41.0
15	30.15433517900029	36.0	27.0	40.0	2.0	41.0
16	30.499493610998623	36.0	27.0	40.0	2.0	41.0
17	30.69869012441212	37.0	29.0	40.0	2.0	41.0
18	30.550824135069142	37.0	28.0	40.0	2.0	41.0
19	30.557924807447886	37.0	27.0	40.0	2.0	41.0
20	30.480076018491417	37.0	27.0	40.0	2.0	41.0
21	30.385340379210387	37.0	27.0	40.0	2.0	41.0
22	30.461936627200668	37.0	28.0	40.0	2.0	41.0
23	30.474698191353298	37.0	29.0	40.0	2.0	41.0
24	30.364153833200355	37.0	29.0	40.0	2.0	41.0
25	30.178671921672088	37.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7291660617874065
1101	2	0.9022635209535821
1101	3	1.575402228778561
1101	4	1.025009514449458
1101	5	0.49584113894419346
1101	6	0.3914612889107474
1101	7	0.6312555057822742
1101	8	0.821943137366496
1101	9	0.9033236765468189
1101	10	1.398568978786777
1101	11	0.659747540916193
1101	12	1.0338183406619947
1101	13	0.7779355938698274
1101	14	1.2033839595796643
1101	15	0.4662592146491207
1101	16	0.01716785920343611
1101	17	-0.4142187943185185
1101	18	-5.934687066832716
1101	19	-7.546886441143698
1101	20	-8.642574544334959
1101	21	-10.3203480377224
1101	22	-10.410095066350607
1101	23	-12.098236065013843
1101	24	-11.516707318425123
1101	25	-12.08448613166997
1102	1	1.8090188365030428
1102	2	2.0247461624580794
1102	3	2.6556188596186665
1102	4	2.197030527709174
1102	5	1.4219939801204262
1102	6	1.529971082041488
1102	7	1.8131924512214788
1102	8	2.076572851222082
1102	9	2.24369786277407
1102	10	-1.3778478286372504
1102	11	0.8987680402291325
1102	12	1.8949324681151118
1102	13	2.387550165056858
1102	14	2.906027303258263
1102	15	2.4416336169159436
1102	16	2.1876345956470935
1102	17	1.899546351903318
1102	18	2.089957931822724
1102	19	1.938579425456414
1102	20	2.1832992646758242
1102	21	2.2912776202744496
1102	22	2.062127929697578
1102	23	2.0875259889994595
1102	24	2.224238988918458
1102	25	2.259835467113845
1103	1	-0.48992580621199266
1103	2	-0.16569318204506445
1103	3	-2.58556517289907
1103	4	-0.6789472387777238
1103	5	-3.3707317677814075
1103	6	-7.040474016729917
1103	7	-8.10432011328448
1103	8	-2.703626956560875
1103	9	-7.088963731158241
1103	10	-5.003663679809836
1103	11	-8.907774685212694
1103	12	-2.9057115202049495
1103	13	-0.9361874158242784
1103	14	-0.09570815956243095
1103	15	-0.7101823444447568
1103	16	-0.5886008885990641
1103	17	-0.8889475602032952
1103	18	-0.6489017436120612
1103	19	-5.706066962515941
1103	20	-1.9926925814036203
1103	21	-6.688442869205648
1103	22	-2.953196176401338
1103	23	-2.0367839579265983
1103	24	-7.33365568348615
1103	25	-3.3962747699742515
1104	1	1.7160481477584923
1104	2	1.853377911201168
1104	3	2.572374888690568
1104	4	2.0964862088560174
1104	5	1.5998371830854872
1104	6	1.5021548122835036
1104	7	1.6462243704653758
1104	8	1.9810310763070227
1104	9	2.282921984504931
1104	10	2.5721777539873294
1104	11	2.1072756049576746
1104	12	2.3919906197638348
1104	13	2.7316009616924894
1104	14	3.0797168511343997
1104	15	2.1584738322970196
1104	16	1.444203730021254
1104	17	1.244880338336511
1104	18	1.2752857492316068
1104	19	1.6852411845519235
1104	20	-8.670079179931978
1104	21	-3.016716852705411
1104	22	-0.5223380796537747
1104	23	0.4864260555122364
1104	24	0.9685493702390566
1104	25	1.4358615227151432
1105	1	1.3382038195663633
1105	2	1.3293449251934781
1105	3	2.1037219831876897
1105	4	1.4994766017957808
1105	5	1.2390415461414968
1105	6	1.1443405851320172
1105	7	1.5276410332357102
1105	8	1.6948830075950774
1105	9	1.6907042526446538
1105	10	2.332109413910729
1105	11	1.858218733183989
1105	12	2.135334196247836
1105	13	2.143069523607842
1105	14	2.8076352459891183
1105	15	2.1847725752425546
1105	16	1.7200004595286025
1105	17	0.978802583539899
1105	18	1.5784400471223137
1105	19	1.4719482803794754
1105	20	1.5755640277249903
1105	21	1.42367437630773
1105	22	1.4113328964244722
1105	23	1.4119651704391352
1105	24	1.3438641041409234
1105	25	1.3311306347698846
1106	1	1.0771474320631782
1106	2	1.2767802174638518
1106	3	1.734496735936169
1106	4	1.4117258198018199
1106	5	0.6550982475419538
1106	6	0.4981586964894049
1106	7	1.0602004624799939
1106	8	1.0559938364383825
1106	9	1.3440448782314718
1106	10	-2.3875636902715662
1106	11	-0.02490053984385554
1106	12	0.5727883905626356
1106	13	1.6442589924171038
1106	14	0.7184761023008654
1106	15	1.455545022829714
1106	16	0.9124206687219925
1106	17	-5.609551632893869
1106	18	-1.5459404581729466
1106	19	0.056744043225702256
1106	20	0.8003220588666622
1106	21	0.9104494180365776
1106	22	1.187774426132865
1106	23	0.6230860704431578
1106	24	0.978910086958841
1106	25	1.096892488498213
1107	1	0.8330806221259621
1107	2	1.2606157897840475
1107	3	1.6653869716961616
1107	4	1.2132285901873239
1107	5	0.7428197981423494
1107	6	0.9358198224768941
1107	7	0.8981604261632405
1107	8	1.1021718643051983
1107	9	1.1690220562828948
1107	10	-2.0655912127081564
1107	11	0.12779424085536917
1107	12	0.6270439487247508
1107	13	1.5344213998361695
1107	14	1.8228480672089944
1107	15	1.4752806532671912
1107	16	-5.195988029501937
1107	17	-1.8391067553899099
1107	18	0.023932172831649723
1107	19	0.812806444018932
1107	20	1.2564206469294916
1107	21	1.339864819377091
1107	22	0.9970683418364992
1107	23	0.7118765023472413
1107	24	1.001180823662498
1107	25	1.220946154205258
1108	1	-0.5158106696815636
1108	2	-3.394190168521991
1108	3	-0.24793996207336022
1108	4	-0.6866711674203998
1108	5	-0.9035486410925344
1108	6	-0.5063362712199755
1108	7	-0.261063815716458
1108	8	0.05136968836329103
1108	9	-0.3351186933456276
1108	10	-3.199236188741594
1108	11	-1.2321639707326675
1108	12	-5.797004645019815
1108	13	-1.5828803991818923
1108	14	-5.5991418227611796
1108	15	-2.393659049337124
1108	16	-7.299269425241935
1108	17	-3.039545126413149
1108	18	-1.6622271376067808
1108	19	-0.5116690323861519
1108	20	-0.4969060730401118
1108	21	-0.0349611468124813
1108	22	-0.15418698167998812
1108	23	-0.2053556628491897
1108	24	0.08361902770529284
1108	25	-0.17660133068903505
1109	1	-9.011426464395406
1109	2	-6.479667147234473
1109	3	-5.318041405164685
1109	4	-5.142223529548531
1109	5	-2.392176846032104
1109	6	-1.5385755232386202
1109	7	-0.9360153012324091
1109	8	-0.5726952613729246
1109	9	-5.429575110418149
1109	10	-1.3665011816381245
1109	11	-1.1259405474329647
1109	12	-5.831667348784734
1109	13	-7.675067041391049
1109	14	-7.794789628042757
1109	15	-3.6351026311202013
1109	16	-2.0994360502196585
1109	17	-1.7009177225552037
1109	18	-1.1757205237788035
1109	19	-0.8776188127426501
1109	20	-0.6844784523996701
1109	21	-0.5357618687668584
1109	22	-0.8711046157753302
1109	23	-1.1351048589065584
1109	24	-0.8138838537362609
1109	25	-0.6915855788085352
1110	1	-2.0674016227326035
1110	2	-1.9129691129194022
1110	3	-1.2436393627962694
1110	4	-2.096890271951974
1110	5	-4.82498911034083
1110	6	-3.6252603036177433
1110	7	-8.030396263531273
1110	8	-9.070571728378514
1110	9	-10.571662479101622
1110	10	-8.429516956977597
1110	11	-11.374982003851184
1110	12	-6.099265704034398
1110	13	-4.365701179112065
1110	14	-3.8620939983703515
1110	15	-9.122802094966932
1110	16	-10.725370279140378
1110	17	-11.414542958049243
1110	18	-8.133734705490266
1110	19	-11.155885513490865
1110	20	-12.391792920964175
1110	21	-13.22664751570094
1110	22	-13.98916392398299
1110	23	-14.614780200589871
1110	24	-14.85425685736216
1110	25	-15.575671262348726
1111	1	-0.676556890212499
1111	2	-0.027068660042729675
1111	3	-2.830869531775676
1111	4	-3.3810209688346795
1111	5	-1.1011745871300604
1111	6	-0.7207279475747725
1111	7	-0.35854902073489114
1111	8	0.056954843136441724
1111	9	-4.402215963739486
1111	10	-3.9256671687595706
1111	11	-1.3245505259687071
1111	12	-5.765303243769225
1111	13	-1.2867332862710263
1111	14	0.09549090697022322
1111	15	0.14432036469005283
1111	16	-0.012109709172314354
1111	17	-0.16259905802558805
1111	18	0.14099647609934607
1111	19	0.21661583187863442
1111	20	0.37187956014145485
1111	21	0.4904455190613142
1111	22	0.1525190005300061
1111	23	0.08127771099286818
1111	24	0.29038687707947375
1111	25	0.4774691503575461
1112	1	-0.24833483903962872
1112	2	0.26170267790893575
1112	3	-2.4303738525914724
1112	4	-0.5035609971124124
1112	5	-0.5590213851407917
1112	6	-0.35708066589470633
1112	7	-0.021358888462788883
1112	8	-3.5162410866168656
1112	9	-0.9590785001102198
1112	10	-3.589488320285639
1112	11	-0.9159618468882265
1112	12	0.15046950806910075
1112	13	0.6186809716022488
1112	14	1.2116588919906661
1112	15	0.6348516912869542
1112	16	0.3807146795537122
1112	17	0.17128290700391702
1112	18	0.296155524701021
1112	19	0.49269357926785773
1112	20	0.6793098347848421
1112	21	0.4136015979550969
1112	22	0.3834601904875896
1112	23	0.1618801686445046
1112	24	0.21300114805440273
1112	25	0.2543548329809333
1113	1	-1.5662802005863483
1113	2	-1.26192431797174
1113	3	-3.542353605567829
1113	4	-2.0881047093458207
1113	5	-2.21360095053576
1113	6	-2.010834047281154
1113	7	-1.9152000416470933
1113	8	-9.256822984899781
1113	9	-9.903280673927341
1113	10	-4.410948570115085
1113	11	-9.741381048298368
1113	12	-8.9104627646412
1113	13	-3.949972345635441
1113	14	-2.354248021028365
1113	15	-8.139021660729096
1113	16	-4.1266055766340415
1113	17	-3.1920632598530077
1113	18	-2.563666420058638
1113	19	-2.5269603228888755
1113	20	-7.268219985338408
1113	21	-3.7348122758268403
1113	22	-3.3583925207875467
1113	23	-3.3657812978618082
1113	24	-8.320355359537668
1113	25	-5.696482834447902
1114	1	-4.510802746558731
1114	2	-4.4312426020670586
1114	3	-1.31061818164126
1114	4	-0.9070900619042774
1114	5	-1.0270797772606173
1114	6	-1.0475233290336128
1114	7	-0.7951243721258834
1114	8	-0.37751377297487565
1114	9	-0.6606542281364582
1114	10	-3.332260053671707
1114	11	-1.450089834941128
1114	12	-4.694842176833028
1114	13	-1.5829655047882554
1114	14	-5.9310635732573935
1114	15	-2.298043059013942
1114	16	-1.4426539313575617
1114	17	-1.2422012197907897
1114	18	-0.9682796987785736
1114	19	-0.5473525520752034
1114	20	-0.377292266185858
1114	21	-0.31266701397904484
1114	22	-0.2851646999803066
1114	23	-0.8341476942775543
1114	24	-0.669402284690328
1114	25	-0.5981638087868575
1115	1	0.6469442348276822
1115	2	0.8049114691177266
1115	3	1.402467658838276
1115	4	0.31600214562720197
1115	5	0.2125897096467746
1115	6	-0.3746080796574134
1115	7	-2.3525006878398855
1115	8	-0.08361054069219875
1115	9	-0.03969423731419042
1115	10	0.8524348154022015
1115	11	-0.01864199027425073
1115	12	0.7577029804790456
1115	13	0.9170054706326276
1115	14	1.5685326065114396
1115	15	0.7128381753910844
1115	16	0.3404825587333562
1115	17	0.056316461684531305
1115	18	0.3484881332689227
1115	19	0.5437278391256442
1115	20	0.5597834787878995
1115	21	0.6381781978258161
1115	22	0.23086948707335608
1115	23	0.2021544825658772
1115	24	-0.0391002799293787
1115	25	0.1992053941844354
1116	1	0.40496580165789453
1116	2	0.6436539072478844
1116	3	1.485191333826144
1116	4	0.8459371216341012
1116	5	-0.09317650591369642
1116	6	-0.9539457623015046
1116	7	-4.573270084507808
1116	8	-0.9403385290461124
1116	9	-1.1118752908538454
1116	10	-0.39148581866260557
1116	11	-2.232853021707907
1116	12	-0.5532676347147856
1116	13	-6.388206046054705
1116	14	-1.595953294173306
1116	15	-1.2523710767790774
1116	16	-0.8277883206541468
1116	17	-0.4187633895043419
1116	18	-0.3179945323912925
1116	19	-0.042428951730016706
1116	20	0.02333176152904315
1116	21	0.18162619480645859
1116	22	0.0669144539143538
1116	23	-0.09261922746808793
1116	24	-0.027876901485427652
1116	25	0.03086269725231716
1201	1	2.284814506958881
1201	2	2.487335518066917
1201	3	3.129457377630821
1201	4	2.691222338732377
1201	5	2.121063427625586
1201	6	2.02413495275108
1201	7	2.3175517353640345
1201	8	2.456052448525835
1201	9	2.7362285206157964
1201	10	3.0064478664326693
1201	11	2.623937732117497
1201	12	3.0506214976065635
1201	13	2.9654180334231093
1201	14	3.668593949484382
1201	15	3.027731623273027
1201	16	2.6249671682196087
1201	17	2.36303063785779
1201	18	2.4142143746034463
1201	19	2.4219761216788918
1201	20	2.6266241591941366
1201	21	2.6247972923910154
1201	22	1.8524009331713494
1201	23	1.3367313881884613
1201	24	1.8504172701073927
1201	25	1.9830269988039362
1202	1	2.1569080662032754
1202	2	2.370445786652045
1202	3	3.0406366184190006
1202	4	2.640248896697482
1202	5	1.9863314102120029
1202	6	1.8891730803590399
1202	7	2.1763693092654073
1202	8	2.4146150942393163
1202	9	2.612266821235103
1202	10	-0.9646594454567641
1202	11	1.292496361569981
1202	12	2.282781640381234
1202	13	2.861179214100492
1202	14	3.2527104330422105
1202	15	2.7644073208425795
1202	16	2.4963120451510754
1202	17	2.1099923457133585
1202	18	2.375113603659095
1202	19	2.536769873344152
1202	20	2.5116441671191723
1202	21	2.632475316828817
1202	22	2.426195233222632
1202	23	2.023907030671367
1202	24	2.170238355291609
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2211	19	0.8955975513568895
2211	20	1.0792944003042138
2211	21	1.1901925791301196
2211	22	1.124011659392199
2211	23	0.8925026870429171
2211	24	1.1209128458642326
2211	25	1.1763856883734185
2212	1	0.7868526445794153
2212	2	0.935753674440921
2212	3	1.5252658024303116
2212	4	0.9332543263473987
2212	5	0.5111631831426351
2212	6	0.41040802412250343
2212	7	0.8165515376130337
2212	8	0.9774301660848081
2212	9	0.7599353757477658
2212	10	1.455112516140293
2212	11	0.7191971955902083
2212	12	0.9616319672828908
2212	13	0.897151075642693
2212	14	-4.451420424130475
2212	15	-1.2083618917079981
2212	16	-0.5975760411324451
2212	17	-0.13357573245548338
2212	18	0.14813932986923461
2212	19	0.5466410576449938
2212	20	0.9310628346367018
2212	21	0.7951067373024436
2212	22	0.6033992343334766
2212	23	0.4629828207452569
2212	24	0.49073197236317156
2212	25	0.5634055731153218
2213	1	0.778332243877454
2213	2	0.912046084502343
2213	3	1.40260566788805
2213	4	0.9849223944959782
2213	5	0.5377599585155508
2213	6	0.14717133210729472
2213	7	0.3782883393094387
2213	8	-4.767474228475006
2213	9	-1.4195149386716572
2213	10	0.33320461178229266
2213	11	0.13628278348140554
2213	12	0.7924213062708994
2213	13	-7.9493135338922585
2213	14	-2.27636558353381
2213	15	-0.7857246074143447
2213	16	-0.2611746810856488
2213	17	0.019812502408512955
2213	18	0.21170872150421616
2213	19	0.5988748979491767
2213	20	0.7372269177019248
2213	21	0.8290548946583947
2213	22	0.4024724282498262
2213	23	0.40907360021293826
2213	24	0.30822155264980466
2213	25	0.481805507306607
2214	1	-0.705302444489206
2214	2	-0.33157619691642637
2214	3	-6.688868204539336
2214	4	-2.2339238505777317
2214	5	-1.4357708216625262
2214	6	-0.9107864477788539
2214	7	-0.4184589421093321
2214	8	-5.960354373709279
2214	9	-1.675646798984019
2214	10	0.0805923106583677
2214	11	-0.4687406620010073
2214	12	0.03369539034621738
2214	13	-6.138692821587483
2214	14	-1.3605850405473845
2214	15	-0.7159587885557848
2214	16	-0.5925724697296424
2214	17	-0.5524994775944059
2214	18	-0.3765858705261458
2214	19	-0.03705963715518834
2214	20	0.2552388867575388
2214	21	0.18662222059621314
2214	22	0.012351460507890266
2214	23	-0.08491952122918889
2214	24	0.07462837282204404
2214	25	0.17134581164173923
2215	1	0.15641835303953755
2215	2	0.460016605939618
2215	3	-2.3833054625082006
2215	4	-0.641560419677365
2215	5	-0.38563536165875334
2215	6	-0.6819303241577082
2215	7	0.02981893035785177
2215	8	0.32030654721833685
2215	9	0.6152944385210013
2215	10	1.2607726765188048
2215	11	0.5624080789132897
2215	12	0.2901721002440887
2215	13	1.0269203558377917
2215	14	0.9578929747585967
2215	15	0.6267076509777674
2215	16	0.4231274995709491
2215	17	0.23583333497249015
2215	18	0.47831637872937094
2215	19	0.40318958829963236
2215	20	0.6494827247849067
2215	21	0.7859723860724053
2215	22	0.5590524810582771
2215	23	0.4175004485086653
2215	24	0.2868882547067493
2215	25	0.34109418580285933
2216	1	0.20716085140131923
2216	2	0.01755562970166835
2216	3	1.1118631075286984
2216	4	-0.3012598766999801
2216	5	-0.43523800611106367
2216	6	-0.597445456213876
2216	7	-1.291954852717513
2216	8	-6.266044203208303
2216	9	-2.090912678705344
2216	10	-0.8262511013002438
2216	11	-1.5631048498706441
2216	12	-0.2511813046218556
2216	13	0.27771833022902115
2216	14	1.0670931475161645
2216	15	0.6370698198736946
2216	16	-0.03186179506655762
2216	17	-0.05169051555892068
2216	18	0.1439623711392528
2216	19	0.20553548189078086
2216	20	0.3726634702005214
2216	21	0.18763504625862026
2216	22	0.1310308224104091
2216	23	-0.2261225676702736
2216	24	-0.22218797851246208
2216	25	-0.2687858037545965
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	233136.0
3	4.0
4	1484.0
5	10465.0
6	17027.0
7	15576.0
8	13764.0
9	11296.0
10	10022.0
11	9149.0
12	8444.0
13	8055.0
14	7967.0
15	7946.0
16	8281.0
17	8530.0
18	10036.0
19	13369.0
20	18610.0
21	24200.0
22	28990.0
23	33898.0
24	38669.0
25	43402.0
26	47742.0
27	51822.0
28	56141.0
29	64978.0
30	73313.0
31	82547.0
32	103005.0
33	128865.0
34	162474.0
35	202590.0
36	255283.0
37	299096.0
38	383954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27358580516785	19.030695149774484	13.939605823376464	21.756113221681204
2	13.973596207200654	21.234116204368288	42.10505352533361	22.687234063097453
3	16.017902690835754	28.34179605530793	30.963301737114683	24.676999516741624
4	10.27901287225524	17.78954526615671	38.67587588876504	33.25556597282301
5	11.908044422875253	40.04952060863749	33.45494171891964	14.58749324956761
6	28.728448787228388	38.860248445956024	18.30596840556315	14.105334361252437
7	27.22878957774621	34.70598928881117	20.78860283175647	17.27661830168615
8	25.612990253448935	35.53927778836865	20.139896027136224	18.707835931046187
9	27.21891468291394	17.655395918689095	20.15442363217285	34.971265766224114
10	13.450635945027035	32.12690070818039	32.36394062043496	22.058522726357616
11	32.5755110856037	26.206763692259926	22.252730126772477	18.9649950953639
12	22.650152547539896	29.347685323342066	29.198878073774615	18.80328405534343
13	26.904675249989445	24.383406290906677	26.781093096812725	21.930825362291156
14	20.59540705748357	24.1703187695634	29.51388455989965	25.720389613053378
15	23.423101701826816	30.699318191506393	24.420237575313013	21.457342531353778
16	21.352446984351687	31.718459057401276	25.809783111404244	21.11931084684279
17	20.64667723765131	30.853374437654047	26.40612125157992	22.093827073114728
18	20.095340891542698	29.011491738302364	30.02365425696196	20.869513113192976
19	21.5603960045652	28.621094023335925	30.20640720523052	19.612102766868357
20	22.124730652509907	27.710759878223506	30.08504692327404	20.079462545992545
21	23.114048087174137	27.149825324367445	29.638797253609233	20.09732933484919
22	22.251741069040722	27.585804732426134	29.47456854827196	20.687885650261187
23	21.74605772552287	27.813217442078837	29.412606833396403	21.02811799900189
24	21.23755079717124	28.07574986779163	29.9713866898844	20.71531264515273
25	21.36169189480025	27.610912772933933	30.196310561393997	20.831084770871822
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13549.0
1	13549.0
2	11274.0
3	8999.0
4	8999.0
5	8999.0
6	12819.0
7	16639.0
8	16639.0
9	16639.0
10	17947.0
11	19255.0
12	19255.0
13	19255.0
14	22285.5
15	25316.0
16	25316.0
17	25316.0
18	37217.5
19	49119.0
20	49119.0
21	49119.0
22	68094.0
23	87069.0
24	87069.0
25	87069.0
26	117698.5
27	148328.0
28	148328.0
29	148328.0
30	174547.0
31	200766.0
32	200766.0
33	200766.0
34	217809.0
35	234852.0
36	234852.0
37	234852.0
38	247623.5
39	260395.0
40	260395.0
41	260395.0
42	275980.0
43	291565.0
44	291565.0
45	291565.0
46	299192.5
47	306820.0
48	306820.0
49	306820.0
50	290743.0
51	274666.0
52	274666.0
53	274666.0
54	243069.0
55	211472.0
56	211472.0
57	211472.0
58	180332.5
59	149193.0
60	149193.0
61	149193.0
62	120865.0
63	92537.0
64	92537.0
65	92537.0
66	71945.0
67	51353.0
68	51353.0
69	51353.0
70	38916.0
71	26479.0
72	26479.0
73	26479.0
74	19366.5
75	12254.0
76	12254.0
77	12254.0
78	9888.0
79	7522.0
80	7522.0
81	7522.0
82	5447.5
83	3373.0
84	3373.0
85	3373.0
86	2586.0
87	1799.0
88	1799.0
89	1799.0
90	1244.0
91	689.0
92	689.0
93	689.0
94	389.0
95	89.0
96	89.0
97	89.0
98	60.5
99	32.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34051953987963735
2	0.34392754186830676
3	0.3575194556819412
4	0.35844162092593407
5	0.36501706005701384
6	0.3028310472990582
7	0.3110503462129079
8	0.3092060157249221
9	0.30964705127639697
10	0.4242762005188182
11	0.3090055450197063
12	0.3084041329040587
13	0.30170841134984944
14	0.3072414028138068
15	0.30102681095211553
16	0.3047154719280872
17	0.29866125663056853
18	0.2996636101566478
19	0.30038530469542485
20	0.4893890855729252
21	0.30154803478567677
22	0.3040338715303533
23	0.2979395620917915
24	0.30170841134984944
25	0.2949325015135538
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2494130.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.94830099748628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.47258484202219	49.7951535730322
2	8.14674954835633	9.604740890552886
3	2.9236934858764454	5.170402908895038
4	1.3819407086443911	3.258522274153962
5	0.827152499630691	2.4379617259526567
6	0.4939110908550668	1.7469131789832761
7	0.32421493604267776	1.337834374640717
8	0.24038215999267115	1.1336095937339106
9	0.15965984175931305	0.8470508768315113
>10	0.8803961627589664	9.577638420512404
>50	0.08504999384574155	3.512187015158564
>100	0.05849791825264987	6.375043415097405
>500	0.004238259394363239	1.7069272830532198
>1k	0.0011811542574450065	1.3883221545646658
>5k	2.0843898660794234E-4	0.8643172678342668
>10k+	1.3895932440529489E-4	1.2433750470033964
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	19260	0.772213156491442	No Hit
TATCAACGCAGAGTACTTTTTTTTT	11098	0.44496477729709355	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8715	0.349420439191221	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6940	0.27825333883959535	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5448	0.21843288040318667	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4516	0.18106514095095283	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4101	0.16442607241803756	No Hit
GTGGTATCAACGCAGAGTACATGGG	3532	0.14161250616447418	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3154	0.12645692085015617	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.0094141043169365E-5	0.0
11	0.0	0.0	0.0	4.0094141043169365E-5	0.0
12	0.0	0.0	0.0	8.018828208633873E-5	0.0
13	0.0	0.0	0.0	1.2028242312950809E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	35	0.0021803444	16.274899	2
CGCATAT	170	0.0	16.207514	8
GGTATCA	3955	0.0	13.874751	1
CTAGACG	50	0.0015072863	13.291436	3
TCATCGC	140	3.6379788E-12	12.90091	4
TTCGCGG	75	1.469044E-5	12.675346	16
GTTATCA	650	0.0	12.561136	1
TCGCGGT	70	1.0844512E-4	12.222654	17
GCGTATC	70	1.0979896E-4	12.20642	1
TAACGCA	165	0.0	12.098462	4
TGGTATC	1055	0.0	12.058338	2
CTCAACG	135	3.765308E-10	11.955259	3
ACTGCGT	65	8.0196117E-4	11.693485	8
TTGACCG	155	2.5465852E-11	11.6529045	18
GTGGTAT	1110	0.0	11.461083	1
CGAAAGC	75	2.0616098E-4	11.407811	19
AAATTCG	75	2.0627677E-4	11.407121	14
CATCGCA	135	4.6875357E-9	11.266293	5
TCAACTC	1195	0.0	11.216207	5
CGCAGAT	960	0.0	11.183417	8
>>END_MODULE