FastQCFastQC Report
Thu 26 May 2016
SRR1364328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364328_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494130
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT410941.6476286320280018No Hit
GGTATCAACGCAGAGTACTTTTTTT290681.165456491842847No Hit
TATCAACGCAGAGTACTTTTTTTTT284111.1391146411774848No Hit
ACGCAGAGTACTTTTTTTTTTTTTT138370.5547826296143344No Hit
GTACTTTTTTTTTTTTTTTTTTTTT91000.36485668349284117No Hit
GAGTACTTTTTTTTTTTTTTTTTTT80940.32452197760341284No Hit
GTGGTATCAACGCAGAGTACATGGG58350.23394931298689323No Hit
GTGGTATCAACGCAGAGTACTTTTT54550.21871353939048885No Hit
ATCAACGCAGAGTACTTTTTTTTTT45220.18130570579721186No Hit
GCAGAGTACTTTTTTTTTTTTTTTT32650.13090737050594795No Hit
GGTATCAACGCAGAGTACATGGGAT29630.11879893991091081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC2450.015.5101723
TATAACG2550.015.2738662
TAACGCA2700.015.1311154
TGGTATC17950.014.3949362
GGTATCA67400.014.1665371
GTGGTAT19200.013.6573161
CGAGTAC2150.012.81341311
TTCGCGG1001.4487159E-712.34675416
AGGACCG1405.2750693E-1112.2184255
TACACCG701.0879636E-412.2184255
CCGAGTA2150.011.931643510
TCGCGGT802.8757115E-511.8718817
CAACCGT1302.6120688E-911.69415956
CGGCCCC2150.011.48535316
CGCGCCA752.0724961E-411.40134810
CTACACG2000.011.4011194
ACGCCGA2350.011.3209417
TTTGCGC4300.011.26448217
ACAACCG1354.7075446E-911.2630755
TACACGG2200.011.2310785
Produced by FastQC (version 0.11.5)