##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364328_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2494130 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.46030399377739 31.0 30.0 34.0 26.0 34.0 2 29.493453428650472 31.0 30.0 34.0 25.0 34.0 3 29.509544410275325 31.0 30.0 34.0 25.0 34.0 4 32.817706775508896 35.0 33.0 37.0 26.0 37.0 5 32.39953931831941 35.0 32.0 37.0 25.0 37.0 6 32.27022729368557 35.0 32.0 37.0 23.0 37.0 7 31.997008175195358 35.0 32.0 37.0 19.0 37.0 8 31.869097039849567 35.0 32.0 37.0 17.0 37.0 9 32.52454603408804 37.0 32.0 39.0 11.0 39.0 10 32.67300661954268 37.0 32.0 39.0 17.0 39.0 11 32.60952877356032 37.0 32.0 39.0 15.0 39.0 12 32.57468977158368 37.0 32.0 39.0 15.0 39.0 13 32.58978040438951 37.0 32.0 39.0 14.0 39.0 14 33.47351461230971 38.0 32.0 41.0 13.0 41.0 15 33.50055770950191 38.0 32.0 41.0 13.0 41.0 16 33.459121216616616 38.0 32.0 41.0 11.0 41.0 17 33.48733666649292 38.0 32.0 41.0 11.0 41.0 18 33.465296115278676 38.0 32.0 41.0 10.0 41.0 19 33.41456259296829 39.0 32.0 41.0 10.0 41.0 20 33.3502499869694 39.0 32.0 41.0 10.0 41.0 21 33.30806132799814 39.0 32.0 41.0 8.0 41.0 22 33.254436216235725 39.0 32.0 41.0 2.0 41.0 23 33.01218460946302 38.0 32.0 41.0 2.0 41.0 24 32.9239947396487 38.0 32.0 41.0 2.0 41.0 25 32.75702469398147 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3646776075227933 1101 2 0.5562775013805528 1101 3 -1.4048171300407795 1101 4 -0.36004783316672473 1101 5 0.4689511351342617 1101 6 0.7476470252624523 1101 7 0.8848196801020194 1101 8 0.8538743574101204 1101 9 1.7702998280892999 1101 10 1.4095662349401366 1101 11 1.0759043326094044 1101 12 1.2668038659644907 1101 13 1.1738397720823812 1101 14 1.2720354768355264 1101 15 1.2660112562838677 1101 16 1.3936779702403541 1101 17 1.2166218332262417 1101 18 1.1548635049220408 1101 19 1.2148427031005582 1101 20 1.4886011892505877 1101 21 1.352666358528701 1101 22 1.5471793467678836 1101 23 1.784234233218001 1101 24 1.8258192200784222 1101 25 1.9158010102080993 1102 1 0.5937474946193717 1102 2 0.6272813946410842 1102 3 0.6786929672509352 1102 4 0.6593539418826424 1102 5 0.8245858167629976 1102 6 0.7583861108579057 1102 7 0.9734683212983768 1102 8 1.068211695813936 1102 9 1.5601838603201301 1102 10 1.2942801274786362 1102 11 1.0990850946264672 1102 12 1.2899439073644956 1102 13 1.118949730540244 1102 14 1.1274661289850911 1102 15 1.183354017849517 1102 16 1.1854738816345645 1102 17 1.1577694553411888 1102 18 1.2896388692471987 1102 19 1.261443789438502 1102 20 1.3553506094970231 1102 21 1.5555511832836473 1102 22 1.5267118001758249 1102 23 1.822547856730388 1102 24 1.7431500426149924 1102 25 1.7421230302690773 1103 1 -2.1210826014279327 1103 2 -2.026590799387943 1103 3 -2.011803919934799 1103 4 -1.8643524190324108 1103 5 -4.029356163072578 1103 6 -8.957296718772906 1103 7 -9.94152644476613 1103 8 -10.83731178955859 1103 9 -11.62625198264956 1103 10 -9.179122297884216 1103 11 -11.453167946259445 1103 12 -10.721057151665725 1103 13 -12.296581896509714 1103 14 -11.804320693740596 1103 15 -12.24797613635263 1103 16 -6.890433486889592 1103 17 -4.591933342824792 1103 18 -3.9272664363105676 1103 19 -3.712302055551305 1103 20 -8.59938288830228 1103 21 -5.561371262647654 1103 22 -11.210107558651774 1103 23 -12.228666967244099 1103 24 -13.275671311841812 1103 25 -13.464128920978801 1104 1 0.30939101205187924 1104 2 0.5112434389073108 1104 3 0.6194446864640142 1104 4 0.7622353823620927 1104 5 0.6020063419745156 1104 6 0.7877137370024769 1104 7 0.6073011223225819 1104 8 0.8060203184100985 1104 9 1.3402027561971082 1104 10 1.0733988396559297 1104 11 0.8927368248283756 1104 12 0.7630488644729283 1104 13 0.7513640106719279 1104 14 0.9148010211270972 1104 15 0.8667469211933039 1104 16 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1110 13 -0.175355277762705 1110 14 0.17644104210229727 1110 15 0.3612678454908007 1110 16 -0.21086896454977477 1110 17 -0.02067346814713744 1110 18 0.23255456510135275 1110 19 -0.11864401986827744 1110 20 0.27459230394033796 1110 21 0.20676138767498742 1110 22 0.42630826883745954 1110 23 0.554908103643541 1110 24 0.5636173613048996 1110 25 0.6790979544806532 1111 1 -0.17109473188230595 1111 2 -0.08691150549591597 1111 3 -0.18337475601192565 1111 4 -0.28532886284530434 1111 5 -0.1832592811769942 1111 6 -0.18489176954752296 1111 7 -0.11161158900300805 1111 8 -0.039350861174479945 1111 9 0.6005551662315511 1111 10 0.07464334173731402 1111 11 0.33922568120852503 1111 12 0.3146029183443275 1111 13 0.1784524731110082 1111 14 0.04833818632964437 1111 15 0.06395676490546975 1111 16 0.10660321639949899 1111 17 0.0012851555404864712 1111 18 -0.02030124493821006 1111 19 0.11828632924981264 1111 20 0.4089674854312477 1111 21 0.32811683217784093 1111 22 0.35654871048257775 1111 23 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-0.40855632886729154 2215 5 -0.3755496005341996 2215 6 -0.3340833302121311 2215 7 -0.39281986101505595 2215 8 -0.49113509896251273 2215 9 0.4329839136985072 2215 10 0.2587993044558772 2215 11 0.31426609900174896 2215 12 0.2612045683490152 2215 13 0.18912744489188782 2215 14 -0.12644888541405663 2215 15 -0.550490399099786 2215 16 -0.10713792704187597 2215 17 -0.16281835884596063 2215 18 -0.11729870193818215 2215 19 -0.10965046850667903 2215 20 0.057951680577581044 2215 21 -0.04273535066917589 2215 22 -0.2776845104989505 2215 23 0.23098536782718782 2215 24 0.48717855809086785 2215 25 0.3857789712606987 2216 1 -0.755074071466975 2216 2 -0.7374442198598494 2216 3 -0.8300276934876756 2216 4 -0.7029430657816889 2216 5 -1.4787577926770616 2216 6 -2.025097177099166 2216 7 -1.2868264787162218 2216 8 -2.198956671582014 2216 9 -0.933735856355213 2216 10 -1.4479561121772058 2216 11 -0.9980648228508571 2216 12 -1.9925167934922392 2216 13 -1.8113661012287778 2216 14 -2.016883289225234 2216 15 -1.6960759920647313 2216 16 -2.6856242439766724 2216 17 -2.775729599826999 2216 18 -2.523769576694203 2216 19 -3.730591607410073 2216 20 -2.604377795788274 2216 21 -2.8034481596091183 2216 22 -2.7574912795820055 2216 23 -2.216302767617549 2216 24 -2.506215786464118 2216 25 -2.5771657568301016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 98427.0 3 0.0 4 1309.0 5 13662.0 6 18987.0 7 15286.0 8 11878.0 9 9573.0 10 8218.0 11 7348.0 12 6528.0 13 5879.0 14 5744.0 15 5867.0 16 6009.0 17 6322.0 18 7342.0 19 9664.0 20 13166.0 21 17385.0 22 21041.0 23 24353.0 24 27999.0 25 31071.0 26 34973.0 27 37832.0 28 41452.0 29 48211.0 30 58778.0 31 72617.0 32 88899.0 33 110758.0 34 138039.0 35 172390.0 36 225650.0 37 331609.0 38 759864.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.06231321196021 20.069758264393204 14.216544988746596 22.651383534899985 2 15.693634642479392 21.256656725849147 39.72713137516068 23.322577256510783 3 18.639297259998806 27.241118421184517 29.808594042875992 24.310990275940682 4 11.75440122950146 18.219107392525878 37.12954873238736 32.8969426455853 5 12.204637402842636 39.46517174979314 33.622233479287466 14.707957368076755 6 29.144553678872704 39.15224869788169 17.40485784665379 14.298339776591822 7 26.770188054095478 34.636233448067465 20.56612331372707 18.027455184109982 8 26.420977739846897 35.51008572950288 18.831654784745798 19.23728174590443 9 26.69853166197863 16.170729741014355 20.055454236617845 37.07528436038917 10 15.905945943344236 31.620127372388957 30.397583470307985 22.076343213958822 11 33.09721763182637 24.6916323590285 22.01936683555758 20.191783173587556 12 23.485167745756385 28.968456524118828 28.61066680887902 18.935708921245762 13 28.619623255498784 22.97471062139467 26.20603989252826 22.199626230578286 14 21.913895205257823 23.10044142630939 28.877717013217126 26.10794635521566 15 24.117315669288338 29.441389279277292 25.01095649279959 21.43033855863478 16 21.368318866742023 30.2371339428455 26.728903684058785 21.665643506353685 17 20.703207076580078 29.32478166376621 27.794383004659444 22.177628254994268 18 19.979621060803584 26.826816310048596 31.875556140127863 21.318006489019954 19 21.587731501068106 26.700234313187266 32.0826203617077 19.62941382403693 20 21.775125244765146 25.766035199663794 32.293680477776746 20.16515907779431 21 22.693285032542484 25.156938139406382 31.91056119995974 20.239215628091394 22 21.798752125236582 25.69856125493215 31.943147916466174 20.559538703365092 23 21.25712787204976 25.625735041443004 32.12854368667316 20.988593399834077 24 21.108074470405583 26.138419492667154 32.268450800005134 20.48505523692213 25 21.085233420992637 25.60177469953409 32.40592679645381 20.90706508301946 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3034.0 1 3034.0 2 3246.0 3 3458.0 4 3458.0 5 3458.0 6 7921.5 7 12385.0 8 12385.0 9 12385.0 10 13979.5 11 15574.0 12 15574.0 13 15574.0 14 16230.5 15 16887.0 16 16887.0 17 16887.0 18 28851.5 19 40816.0 20 40816.0 21 40816.0 22 63071.5 23 85327.0 24 85327.0 25 85327.0 26 121453.0 27 157579.0 28 157579.0 29 157579.0 30 181033.5 31 204488.0 32 204488.0 33 204488.0 34 221759.5 35 239031.0 36 239031.0 37 239031.0 38 244422.0 39 249813.0 40 249813.0 41 249813.0 42 264758.0 43 279703.0 44 279703.0 45 279703.0 46 293827.0 47 307951.0 48 307951.0 49 307951.0 50 296271.0 51 284591.0 52 284591.0 53 284591.0 54 252720.5 55 220850.0 56 220850.0 57 220850.0 58 189652.5 59 158455.0 60 158455.0 61 158455.0 62 129450.0 63 100445.0 64 100445.0 65 100445.0 66 78183.0 67 55921.0 68 55921.0 69 55921.0 70 42568.5 71 29216.0 72 29216.0 73 29216.0 74 21109.0 75 13002.0 76 13002.0 77 13002.0 78 10807.0 79 8612.0 80 8612.0 81 8612.0 82 6312.5 83 4013.0 84 4013.0 85 4013.0 86 3025.5 87 2038.0 88 2038.0 89 2038.0 90 1452.0 91 866.0 92 866.0 93 866.0 94 468.5 95 71.0 96 71.0 97 71.0 98 37.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026542321370578117 2 0.001443389077554097 3 2.8065898730218557E-4 4 0.004450449655791799 5 0.03532293825903221 6 0.012389089582339334 7 0.021610742022268283 8 0.02830646357647757 9 0.036205009361981934 10 0.03881112852978794 11 0.0401743293252557 12 0.008299487195936057 13 0.0031674371424103794 14 0.006775909836295622 15 0.007016474682554638 16 0.008940993452626767 17 0.010825418081655729 18 0.01519567945536119 19 0.018403210738814738 20 0.016558880250828946 21 0.01254946614651201 22 0.011627300902519115 23 0.008940993452626767 24 0.005853744592302727 25 0.10689098002108952 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2494130.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.505968292717 #Duplication Level Percentage of deduplicated Percentage of total 1 71.9373589428684 29.138923804016294 2 13.321580634218828 10.792070455570812 3 5.440942629243303 6.611719488678645 4 2.8712504543547834 4.6521111945817655 5 1.6656767669644135 3.3734925154287945 6 1.0695073149856653 2.599285763378295 7 0.7202146882365003 2.0421095327959717 8 0.48695710514790985 1.5779735248827593 9 0.36048732051237337 1.3141699177140638 >10 1.8170139734156847 13.349311736762438 >50 0.1722858438148098 4.907284179146452 >100 0.12497852011692817 9.752277070244105 >500 0.007764176927094263 2.1672772248730503 >1k 0.0031853033547053124 2.0411564636101476 >5k 3.9816291933816405E-4 1.1484730702724288 >10k+ 3.9816291933816405E-4 4.532364058043945 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 41094 1.6476286320280018 No Hit GGTATCAACGCAGAGTACTTTTTTT 29068 1.165456491842847 No Hit TATCAACGCAGAGTACTTTTTTTTT 28411 1.1391146411774848 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 13837 0.5547826296143344 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 9100 0.36485668349284117 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 8094 0.32452197760341284 No Hit GTGGTATCAACGCAGAGTACATGGG 5835 0.23394931298689323 No Hit GTGGTATCAACGCAGAGTACTTTTT 5455 0.21871353939048885 No Hit ATCAACGCAGAGTACTTTTTTTTTT 4522 0.18130570579721186 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 3265 0.13090737050594795 No Hit GGTATCAACGCAGAGTACATGGGAT 2963 0.11879893991091081 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.0094141043169365E-5 0.0 0.0 0.0 0.0 3 4.0094141043169365E-5 0.0 0.0 0.0 0.0 4 4.0094141043169365E-5 0.0 0.0 0.0 0.0 5 4.0094141043169365E-5 0.0 0.0 0.0 0.0 6 4.0094141043169365E-5 0.0 0.0 0.0 0.0 7 4.0094141043169365E-5 0.0 0.0 0.0 0.0 8 4.0094141043169365E-5 0.0 0.0 0.0 0.0 9 4.0094141043169365E-5 0.0 0.0 4.0094141043169365E-5 0.0 10 4.0094141043169365E-5 0.0 0.0 8.018828208633873E-5 0.0 11 4.0094141043169365E-5 0.0 0.0 1.2028242312950809E-4 0.0 12 4.0094141043169365E-5 0.0 0.0 1.6037656417267746E-4 0.0 13 4.0094141043169365E-5 0.0 0.0 2.004707052158468E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 245 0.0 15.510172 3 TATAACG 255 0.0 15.273866 2 TAACGCA 270 0.0 15.131115 4 TGGTATC 1795 0.0 14.394936 2 GGTATCA 6740 0.0 14.166537 1 GTGGTAT 1920 0.0 13.657316 1 CGAGTAC 215 0.0 12.813413 11 TTCGCGG 100 1.4487159E-7 12.346754 16 AGGACCG 140 5.2750693E-11 12.218425 5 TACACCG 70 1.0879636E-4 12.218425 5 CCGAGTA 215 0.0 11.9316435 10 TCGCGGT 80 2.8757115E-5 11.87188 17 CAACCGT 130 2.6120688E-9 11.6941595 6 CGGCCCC 215 0.0 11.485353 16 CGCGCCA 75 2.0724961E-4 11.401348 10 CTACACG 200 0.0 11.401119 4 ACGCCGA 235 0.0 11.320941 7 TTTGCGC 430 0.0 11.264482 17 ACAACCG 135 4.7075446E-9 11.263075 5 TACACGG 220 0.0 11.231078 5 >>END_MODULE