FastQCFastQC Report
Thu 26 May 2016
SRR1364327_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364327_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419819
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75490.531687489743411No Hit
GTACTTTTTTTTTTTTTTTTTTTTT48560.34201542590992234No Hit
TATCAACGCAGAGTACTTTTTTTTT46510.32757696579634443No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA40220.28327554427712265No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN35960.25327171984598035No Hit
GGTATCAACGCAGAGTACTTTTTTT32900.2317196769447373No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA20640.14537064231426683No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20470.14417330659753108No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16280.11466250275563293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT250.006074346618.976911
ATAACGC1800.015.2945113
TAACGCA2000.014.7346794
TATAACG1900.013.9898972
GGTATCA16400.013.9497991
GCGCCGA707.217581E-613.5793919
TAGGAGT1351.8189894E-1213.379164
GTATAAC2800.012.8830981
TATCACG905.451311E-712.6575272
TTGACCG1301.9645086E-1012.43080418
TGCGTAG853.9075476E-612.30394214
CTAATCG701.08266264E-412.22404614
GTTATCA3500.012.2050411
GAATGCG701.10322384E-412.19943611
ACCGAGT1209.995347E-911.8760838
GTGGTAT4450.011.7326851
GGACCGA1302.6084308E-911.6937886
TGACCGA1406.730261E-1011.5424819
GTATCAA41150.011.1883661
GTTCGCG855.2844225E-511.18421516
Produced by FastQC (version 0.11.5)