##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364327_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1419819
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.20618754925804	30.0	26.0	31.0	7.0	34.0
2	26.02375443630491	30.0	26.0	31.0	7.0	34.0
3	25.57808213582154	30.0	25.0	31.0	7.0	34.0
4	29.287556371622017	35.0	28.0	37.0	7.0	37.0
5	29.870172888234347	35.0	30.0	37.0	7.0	37.0
6	29.99016916945047	35.0	30.0	37.0	2.0	37.0
7	29.614238152891318	35.0	30.0	37.0	2.0	37.0
8	29.15338927004076	35.0	28.0	37.0	2.0	37.0
9	30.18766969592603	35.0	28.0	39.0	2.0	39.0
10	29.496069569431032	35.0	27.0	39.0	2.0	39.0
11	30.13340010240742	35.0	28.0	39.0	2.0	39.0
12	29.664113524329508	35.0	27.0	39.0	2.0	39.0
13	29.289997527853902	35.0	27.0	39.0	2.0	39.0
14	29.575836779195093	36.0	25.0	40.0	2.0	41.0
15	30.278422813048707	36.0	27.0	40.0	2.0	41.0
16	30.612697111392368	37.0	28.0	40.0	2.0	41.0
17	30.779143679581694	37.0	29.0	40.0	2.0	41.0
18	30.60975377847458	37.0	28.0	40.0	2.0	41.0
19	30.587176957062837	37.0	28.0	40.0	2.0	41.0
20	30.469480969053098	37.0	27.0	40.0	2.0	41.0
21	30.377431912095837	37.0	27.0	40.0	2.0	41.0
22	30.4499489019375	37.0	28.0	40.0	2.0	41.0
23	30.459446591431725	37.0	29.0	40.0	2.0	41.0
24	30.33519906410606	37.0	29.0	40.0	2.0	41.0
25	30.15346956196529	37.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6471297937655898
1101	2	0.836135833774712
1101	3	1.6014166759956723
1101	4	0.9292180978301019
1101	5	0.49605303414076474
1101	6	0.2448063442787003
1101	7	0.7427213385831841
1101	8	1.0537894081686368
1101	9	0.867908413815325
1101	10	1.330646606065617
1101	11	0.6138596330389099
1101	12	0.9928013667744544
1101	13	0.8406056545900711
1101	14	1.3588366986360185
1101	15	0.6107847779179423
1101	16	0.17904350862797713
1101	17	-0.22092657268144222
1101	18	-6.03739715966061
1101	19	-7.554207846148135
1101	20	-8.500988860057138
1101	21	-10.221874152903933
1101	22	-10.470903944128253
1101	23	-11.657422539154844
1101	24	-11.35706531477491
1101	25	-11.979946203282946
1102	1	1.8849452252012888
1102	2	2.0210078070345894
1102	3	2.7372674341066947
1102	4	2.17730789564375
1102	5	1.464168806329603
1102	6	1.4813122209208842
1102	7	1.7566201577163305
1102	8	2.014784501251171
1102	9	2.2238219687509044
1102	10	-1.3108875516223577
1102	11	0.8758582833506559
1102	12	1.8282313054926682
1102	13	2.365546292186334
1102	14	2.8973183655712447
1102	15	2.463880462306811
1102	16	2.104397079392193
1102	17	1.7921547278754275
1102	18	2.018083736511521
1102	19	1.8579762307812864
1102	20	2.227931692347827
1102	21	2.18230791414182
1102	22	2.0452956259219874
1102	23	1.9608524725691403
1102	24	2.22157630005103
1102	25	2.2465883146028283
1103	1	-0.11533279005692165
1103	2	0.3499207412702745
1103	3	-1.577622045038897
1103	4	-0.10647128453754817
1103	5	-2.6711371736089227
1103	6	-6.905531000662496
1103	7	-7.2805647093780195
1103	8	-2.3973829083122915
1103	9	-7.8575921238816875
1103	10	-4.896887590779436
1103	11	-8.4670702745594
1103	12	-2.7345211760871884
1103	13	-0.9618917254212995
1103	14	-0.3767067863255882
1103	15	-0.6649202216368302
1103	16	-0.5107018901240181
1103	17	-0.7303468873540666
1103	18	-0.7268274748127048
1103	19	-5.781135164581212
1103	20	-1.7190143818287282
1103	21	-6.180302296719049
1103	22	-2.6535263986294986
1103	23	-1.4367119571406377
1103	24	-6.439641876664865
1103	25	-3.6639625602315675
1104	1	1.8434436300763153
1104	2	1.9759430606850295
1104	3	2.6553249152973954
1104	4	2.0825886719219433
1104	5	1.7362032595733936
1104	6	1.577817042302705
1104	7	1.6558059994954846
1104	8	2.0673630073132614
1104	9	2.221055374500253
1104	10	2.637083813696215
1104	11	2.2193164514688064
1104	12	2.5984264380105238
1104	13	2.7752111962384696
1104	14	3.120167580343036
1104	15	2.401894195109918
1104	16	1.6819615992621664
1104	17	1.4281680282126459
1104	18	1.425995793863624
1104	19	1.8519713135406626
1104	20	-8.72859590914615
1104	21	-2.8909827969772834
1104	22	-0.3372549307389896
1104	23	0.74201726141834
1104	24	1.2927487327061513
1104	25	1.704341778782954
1105	1	1.6762407324650006
1105	2	1.5423844317863065
1105	3	2.3167223763519047
1105	4	1.7907712025784441
1105	5	1.591536007570383
1105	6	1.6108266728533316
1105	7	1.9261215900664617
1105	8	2.0930461125029147
1105	9	1.9927659512136877
1105	10	2.481092464103103
1105	11	1.9926245249854766
1105	12	2.2494491070263933
1105	13	2.3091677752814626
1105	14	2.7843801556461685
1105	15	2.265860850580559
1105	16	1.7536177671947684
1105	17	1.2140209595102682
1105	18	1.6903935947274888
1105	19	1.7545652570773136
1105	20	1.714939168803756
1105	21	1.5262099849220725
1105	22	1.4889406383827506
1105	23	1.7074617929430254
1105	24	1.6727803172980558
1105	25	1.7035295755023192
1106	1	0.8246918944734318
1106	2	1.2488288217445422
1106	3	1.6754132754216116
1106	4	1.3727377313859996
1106	5	0.6478611470336837
1106	6	0.4385780968683761
1106	7	0.9227130725213399
1106	8	0.9333021211270456
1106	9	1.2132713625721685
1106	10	-1.7717801860947695
1106	11	-0.16271945747446637
1106	12	0.4188668768698989
1106	13	1.4305720739730532
1106	14	0.4154602591519492
1106	15	1.0689319814237805
1106	16	0.6704731204984213
1106	17	-6.088396674568017
1106	18	-1.9584776289224095
1106	19	-0.27402588495134594
1106	20	0.48440638150327686
1106	21	0.96722891302527
1106	22	0.9292928984683826
1106	23	0.2780171537441589
1106	24	0.5729108140163888
1106	25	0.6973097047427039
1107	1	0.8622671244261682
1107	2	1.3699570859568695
1107	3	1.7163450042268593
1107	4	1.1402743961904171
1107	5	0.7716774016793622
1107	6	0.8288935640697943
1107	7	0.9397655996854368
1107	8	0.9724777258490427
1107	9	1.1817723785836023
1107	10	-1.7755711648517796
1107	11	0.15709092481525744
1107	12	0.7109350699877197
1107	13	1.6394992605619443
1107	14	1.8965945672467477
1107	15	1.6030617129160092
1107	16	-5.1179922066702055
1107	17	-1.5951580152419318
1107	18	0.18593859635339882
1107	19	0.905918377021429
1107	20	1.3316824473646065
1107	21	1.3498210346990618
1107	22	1.028147594876561
1107	23	0.7162417167459694
1107	24	1.1341538715412867
1107	25	1.4063590635207532
1108	1	-0.6601948599633296
1108	2	-3.4406465320523267
1108	3	-0.40674562276270976
1108	4	-1.0043825508749649
1108	5	-1.0581791119974184
1108	6	-0.5645367034101838
1108	7	-0.14611220044189466
1108	8	-0.0684380530638542
1108	9	-0.4274917118649917
1108	10	-3.3628020768499987
1108	11	-1.314682760575387
1108	12	-6.022066538852883
1108	13	-1.7701675310367335
1108	14	-6.087289612596429
1108	15	-2.534971246221403
1108	16	-7.369620278074578
1108	17	-3.2691092001047544
1108	18	-1.9814524304731584
1108	19	-0.7403114040420427
1108	20	-0.6086198280891999
1108	21	-0.13966953729860165
1108	22	-0.29678951631347417
1108	23	-0.3427898855970781
1108	24	-0.05731084444024859
1108	25	-0.35029643018708967
1109	1	-8.45131405182654
1109	2	-6.187512467185883
1109	3	-4.931846464626204
1109	4	-4.552377287292387
1109	5	-1.8859866888503696
1109	6	-1.0926843999611435
1109	7	-0.6133067060376121
1109	8	-0.07598499373892409
1109	9	-5.127706110347045
1109	10	-1.1519910303359993
1109	11	-0.6759629489493761
1109	12	-5.585986098465931
1109	13	-7.473546558700292
1109	14	-7.388721611885707
1109	15	-3.099945274704087
1109	16	-1.6390813925228578
1109	17	-1.2095385350008065
1109	18	-0.47951078141833037
1109	19	-0.19632670576073608
1109	20	0.06374192136329171
1109	21	0.04786759834420096
1109	22	-0.0032964959804608895
1109	23	-0.48747951924097066
1109	24	-0.18129784115804526
1109	25	-0.1615424272806294
1110	1	-2.5239037705310814
1110	2	-2.363367023060434
1110	3	-1.7922010763272915
1110	4	-2.5659858831730915
1110	5	-5.586710794122816
1110	6	-4.059847857708267
1110	7	-8.639776095529573
1110	8	-9.715501161181049
1110	9	-11.567109336417957
1110	10	-9.271411207372477
1110	11	-11.698425320694746
1110	12	-6.742251787478569
1110	13	-4.961643404852516
1110	14	-4.618301788547406
1110	15	-9.749839321409421
1110	16	-11.42233897614749
1110	17	-12.327147472867829
1110	18	-8.876094275659607
1110	19	-12.483106045727144
1110	20	-13.460472154262533
1110	21	-14.39507475666102
1110	22	-15.015462776285874
1110	23	-15.657339554704484
1110	24	-15.971893490748561
1110	25	-16.604642697199964
1111	1	-0.4901863199209231
1111	2	0.05743114661477833
1111	3	-2.323968907529288
1111	4	-2.8829207423992145
1111	5	-1.0481632333634359
1111	6	-0.42430303745897646
1111	7	0.10269527842718063
1111	8	0.1765613137390858
1111	9	-3.944924114019809
1111	10	-3.93037556829859
1111	11	-1.1169231416626033
1111	12	-5.925383030226794
1111	13	-1.1354223847357403
1111	14	0.06048072195892118
1111	15	0.083661470258928
1111	16	0.11619691955759137
1111	17	0.174330878346737
1111	18	0.5290976067068733
1111	19	0.5010909163816457
1111	20	0.615882625143513
1111	21	0.7820754239140193
1111	22	0.49970257197043466
1111	23	0.4183316525197611
1111	24	0.6363224402938599
1111	25	0.7044913513826536
1112	1	0.06882179801355903
1112	2	0.7128309196204015
1112	3	-2.1844731684830485
1112	4	-0.16422224094683457
1112	5	-0.2417336497121596
1112	6	0.05461502013603692
1112	7	0.6275283990868772
1112	8	-2.8188563174399377
1112	9	-0.22873281034108928
1112	10	-3.054172466042111
1112	11	-0.3812305202622923
1112	12	0.9365615869927204
1112	13	1.2731290192308897
1112	14	1.9962914894435784
1112	15	1.4458818151108375
1112	16	1.1467217318383724
1112	17	0.9044408167248186
1112	18	0.72393765261873
1112	19	1.0492719155416381
1112	20	1.0437678262643786
1112	21	0.8505264117242959
1112	22	0.9541666344002984
1112	23	0.7780167459144813
1112	24	0.9631775101555462
1112	25	0.9165603666502697
1113	1	-1.090256687258183
1113	2	-0.6522931583293783
1113	3	-3.513817458576895
1113	4	-1.4730672294114484
1113	5	-1.7006078671129714
1113	6	-1.1973930747174393
1113	7	-1.278173697810395
1113	8	-8.881913284859714
1113	9	-9.266056600017695
1113	10	-3.625124035256679
1113	11	-9.347884989612073
1113	12	-7.626833409727393
1113	13	-2.9683120342602933
1113	14	-1.2521527948491027
1113	15	-7.30084443247387
1113	16	-2.997907395354357
1113	17	-2.217998862336998
1113	18	-1.542778621428205
1113	19	-1.238512062538888
1113	20	-6.865460150513119
1113	21	-2.6943734735911775
1113	22	-2.121699746968151
1113	23	-1.7956256704037408
1113	24	-7.721165039589426
1113	25	-4.743024604767847
1114	1	-4.736686023887767
1114	2	-4.379399325064718
1114	3	-1.1673732888199915
1114	4	-0.998139609579006
1114	5	-0.9034953825641452
1114	6	-0.9017034716409356
1114	7	-0.8208101488473396
1114	8	-0.29884227812987163
1114	9	-0.6818110293543249
1114	10	-3.652655418308459
1114	11	-1.5900022811928984
1114	12	-4.893751690183375
1114	13	-1.5415865844594414
1114	14	-6.042537632096732
1114	15	-2.336124201736336
1114	16	-1.4161081919482292
1114	17	-1.265325328481758
1114	18	-0.6912785527563194
1114	19	-0.5891616985778967
1114	20	-0.39660642162972337
1114	21	-0.36101871462949475
1114	22	-0.38130749315686074
1114	23	-0.8636174297774559
1114	24	-0.5496451934144488
1114	25	-0.5335346995350498
1115	1	0.6231022993794184
1115	2	0.7281224849247394
1115	3	1.3812835993019519
1115	4	0.23701061571099657
1115	5	0.03527597205190247
1115	6	-0.5804798113974314
1115	7	-2.299686958357455
1115	8	-0.23598540918154143
1115	9	-0.028248763032785007
1115	10	0.6461036651926442
1115	11	-0.18546235213528206
1115	12	0.5596786616525016
1115	13	0.6940391465347098
1115	14	1.367259319298146
1115	15	0.6207995831356818
1115	16	0.1553058623131065
1115	17	-0.24565473122358839
1115	18	0.32760055758784645
1115	19	0.37523097710037234
1115	20	0.5068712890403937
1115	21	0.5498665312721442
1115	22	0.1657928807286737
1115	23	0.20293879685469918
1115	24	-0.07071086305302643
1115	25	0.36041640774133654
1116	1	0.33256887807301894
1116	2	0.5343000213405134
1116	3	1.3732541621604
1116	4	0.6140695407477921
1116	5	-0.08890714727002802
1116	6	-0.9509322298108707
1116	7	-4.469699037736227
1116	8	-0.8560448925089545
1116	9	-1.078438480334718
1116	10	-0.34436783660911274
1116	11	-1.912302934787668
1116	12	-0.10028763789579287
1116	13	-6.875991813217613
1116	14	-1.6643415536653592
1116	15	-1.2511045938931957
1116	16	-0.7160820481573822
1116	17	-0.2366717337105193
1116	18	-0.06066417367135202
1116	19	0.10751803998419618
1116	20	0.1601778920957635
1116	21	0.39545187360579703
1116	22	0.25296701664706234
1116	23	-0.04353899840490527
1116	24	0.06355742096463501
1116	25	0.00921039850416605
1201	1	1.9232747079283854
1201	2	2.087204869491284
1201	3	2.7228275143928578
1201	4	2.248791224869901
1201	5	1.7462431270297856
1201	6	1.7000475323751267
1201	7	1.9131014977390066
1201	8	1.9748123403440268
1201	9	2.5227351553846304
1201	10	2.6415953625929234
1201	11	2.2867563087223175
1201	12	2.5928846328866832
1201	13	2.737276423126456
1201	14	3.311996950678811
1201	15	2.6966254635916442
1201	16	2.2251769486140205
1201	17	2.1215662377832984
1201	18	2.0912774490285955
1201	19	2.172498274648344
1201	20	2.4563324449476944
1201	21	2.3711919056293205
1201	22	1.3901534617739735
1201	23	0.7569662382278999
1201	24	1.3670901925739471
1201	25	1.5268597672208628
1202	1	2.309924527040188
1202	2	2.5664597036264034
1202	3	3.16795039962075
1202	4	2.7672395126383478
1202	5	2.303091202267069
1202	6	2.0056352199660594
1202	7	2.406011615486044
1202	8	2.6360274183969956
1202	9	2.962982774532861
1202	10	-0.6075434303889296
1202	11	1.4934262345652982
1202	12	2.6335944055835903
1202	13	3.0838266358630264
1202	14	3.753367088055249
1202	15	3.307250926131559
1202	16	2.8667248734714192
1202	17	2.4072479093768777
1202	18	2.7052362312078273
1202	19	2.974563762964312
1202	20	2.8212553349098037
1202	21	3.1132662481061466
1202	22	2.831554117247933
1202	23	2.496625738423628
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2211	16	-0.2525114876571415
2211	17	0.22198276315037901
2211	18	0.6207296388901042
2211	19	0.7954627715845106
2211	20	1.1744185819422128
2211	21	1.1783263346632609
2211	22	1.0487990934983742
2211	23	0.7584988725514492
2211	24	0.997788630465152
2211	25	1.114531595649975
2212	1	0.9213833731543275
2212	2	1.190090625636838
2212	3	1.6936733550935443
2212	4	1.3163765072203866
2212	5	0.9125353341594042
2212	6	0.7697860998418093
2212	7	1.0040654673832243
2212	8	1.3439127465193508
2212	9	1.1415928386775285
2212	10	1.802117808843608
2212	11	1.0821770893038796
2212	12	1.4415778509618669
2212	13	1.561674318081323
2212	14	-3.61596994153928
2212	15	-0.47017084564987144
2212	16	0.002536100808690378
2212	17	0.35155673446856284
2212	18	0.7078230778845587
2212	19	1.1700950325559702
2212	20	1.453602907152984
2212	21	1.2254386232544618
2212	22	1.1684490483675987
2212	23	1.081612376820953
2212	24	1.0235451744277952
2212	25	1.0132354938665422
2213	1	0.7792714106822665
2213	2	0.9904175668919528
2213	3	1.5473750574555325
2213	4	0.9768725656678541
2213	5	0.5620231679104002
2213	6	0.22840716012974482
2213	7	0.42593858090052805
2213	8	-4.644095849216054
2213	9	-1.2482660192881774
2213	10	0.4420484451753879
2213	11	0.25802341145401897
2213	12	0.6787468238612995
2213	13	-7.738230051049413
2213	14	-2.1495655297096192
2213	15	-0.6160425221274757
2213	16	-0.1440901687481535
2213	17	0.22673526014828482
2213	18	0.27540038502529285
2213	19	0.7009427976353173
2213	20	0.745007613457993
2213	21	0.7487448746716261
2213	22	0.43067640100477433
2213	23	0.4182105828771334
2213	24	0.2834737788796744
2213	25	0.5105458296567811
2214	1	-1.3171269687696565
2214	2	-0.9785419322388194
2214	3	-7.284941092844491
2214	4	-2.905177229876468
2214	5	-1.8913588996245139
2214	6	-1.3671872861641212
2214	7	-1.1024304463101586
2214	8	-6.534642803064255
2214	9	-2.3119737590131173
2214	10	-0.5737682792008449
2214	11	-0.9790066556460921
2214	12	-0.6103781118806602
2214	13	-6.533593583584551
2214	14	-1.929260291764276
2214	15	-1.2698809821387833
2214	16	-1.2391528113708041
2214	17	-1.2015003066094927
2214	18	-1.0814062867895657
2214	19	-0.879852862182446
2214	20	-0.5465381801599101
2214	21	-0.5589784696601185
2214	22	-0.7909207914945675
2214	23	-0.8377696607485845
2214	24	-0.47467406480552654
2214	25	-0.373156746779145
2215	1	-0.21332385854501723
2215	2	0.11351231561391373
2215	3	-2.8850373076490605
2215	4	-0.838482455514086
2215	5	-0.45439388212284015
2215	6	-1.1008004609515183
2215	7	-0.5238667341175187
2215	8	-0.06015246740122393
2215	9	0.2814522833847164
2215	10	0.7085951455828834
2215	11	0.04812383433127465
2215	12	-0.09978444491515859
2215	13	0.4683226045177058
2215	14	0.47466389776499085
2215	15	0.08481960526572507
2215	16	-0.28459500179426556
2215	17	-0.31702602476498143
2215	18	-0.3080936944646666
2215	19	-0.1734551182111197
2215	20	0.0804468293264371
2215	21	0.18354615739168523
2215	22	-0.061238060970925545
2215	23	-0.26715023354326917
2215	24	-0.2983685434193539
2215	25	-0.29334170159447837
2216	1	0.02961353691792823
2216	2	-0.22319053108218156
2216	3	0.8739745833593204
2216	4	-0.3734625435988974
2216	5	-0.6695544466259378
2216	6	-0.7084238512663603
2216	7	-1.2905913594081966
2216	8	-6.340149764625906
2216	9	-2.042101200488659
2216	10	-1.0120686143304667
2216	11	-1.6630002114266311
2216	12	-0.32527029283873077
2216	13	0.37404105954075817
2216	14	0.9063789209851976
2216	15	0.40637708555732033
2216	16	-0.14754945602011915
2216	17	-0.2880838725778787
2216	18	-0.051130539227116856
2216	19	0.03414689852443331
2216	20	0.2576095408115897
2216	21	0.08329023584756001
2216	22	-0.09409683133647562
2216	23	-0.3707772056369123
2216	24	-0.4072454761142339
2216	25	-0.29652849023624483
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	135008.0
3	2.0
4	880.0
5	6032.0
6	9470.0
7	8450.0
8	7537.0
9	6240.0
10	5596.0
11	5006.0
12	4756.0
13	4564.0
14	4647.0
15	4436.0
16	4581.0
17	4891.0
18	5601.0
19	7470.0
20	10107.0
21	12840.0
22	15743.0
23	18618.0
24	21347.0
25	23631.0
26	26480.0
27	29133.0
28	31388.0
29	36297.0
30	41548.0
31	46515.0
32	58068.0
33	72347.0
34	91180.0
35	114020.0
36	143132.0
37	170834.0
38	231424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.31047589442152	18.014257050811054	13.019838354151359	22.655428700616064
2	14.272643775611135	21.934435495266356	41.13409636851773	22.658824360604775
3	15.636022927689593	29.106747162302714	30.61568703929815	24.641542870709536
4	10.444674323329362	17.786478316721286	38.407462207769996	33.36138515217935
5	12.099959226562516	39.87232331569311	33.30779988085959	14.719917576884784
6	28.702530528070287	38.44382764441242	18.891737469189426	13.961904358327859
7	27.133455101772707	33.712270501101145	21.880955105304274	17.273319291821878
8	25.329207585123577	35.574195662737736	20.725881182971897	18.37071556916679
9	26.81642924593934	17.692452754228757	19.904424296231003	35.58669370360089
10	13.121812318055087	31.63750244832358	32.902994061091775	22.33769117252956
11	32.39006770605993	25.954460505740112	23.023521251905247	18.631950536294713
12	22.08017855124873	28.407729686970278	30.013490224884166	19.498601536896825
13	26.6754853513549	24.29867797416968	26.885594163000647	22.140242511474767
14	20.598702838767366	23.295789629066697	29.458965251532458	26.64654228063348
15	22.766003577812477	30.704495712769315	24.40250319750217	22.126997511916034
16	21.873949413649218	30.470182417104326	25.52257401071845	22.13329415852801
17	21.075130457627488	30.030996266182292	26.593722920640662	22.30015035554956
18	20.647261761413592	29.03179104003277	28.96060283975946	21.36034435879418
19	22.173585406695352	28.308957248804152	28.896059641071602	20.621397703428894
20	22.563148539177003	27.643761286633794	28.922936704274626	20.870153469914577
21	23.412357620683423	27.156100275718675	28.36109824172844	21.07044386186946
22	22.757526704014147	27.272136575388515	28.44736745457987	21.522969266017462
23	22.109931707592676	27.616474925316215	28.47751011673976	21.79608325035135
24	21.84437975216359	27.722266715821497	28.792302466075398	21.641051065939514
25	21.84331771199679	27.285361988457723	29.034075982406925	21.837244317138556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8261.0
1	8261.0
2	7537.0
3	6813.0
4	6813.0
5	6813.0
6	10136.5
7	13460.0
8	13460.0
9	13460.0
10	13044.0
11	12628.0
12	12628.0
13	12628.0
14	14072.0
15	15516.0
16	15516.0
17	15516.0
18	22066.5
19	28617.0
20	28617.0
21	28617.0
22	37737.0
23	46857.0
24	46857.0
25	46857.0
26	61404.5
27	75952.0
28	75952.0
29	75952.0
30	89652.5
31	103353.0
32	103353.0
33	103353.0
34	112769.5
35	122186.0
36	122186.0
37	122186.0
38	130823.5
39	139461.0
40	139461.0
41	139461.0
42	148351.5
43	157242.0
44	157242.0
45	157242.0
46	163636.5
47	170031.0
48	170031.0
49	170031.0
50	164551.5
51	159072.0
52	159072.0
53	159072.0
54	145041.5
55	131011.0
56	131011.0
57	131011.0
58	114742.5
59	98474.0
60	98474.0
61	98474.0
62	80850.5
63	63227.0
64	63227.0
65	63227.0
66	49543.0
67	35859.0
68	35859.0
69	35859.0
70	27087.5
71	18316.0
72	18316.0
73	18316.0
74	12982.5
75	7649.0
76	7649.0
77	7649.0
78	5636.5
79	3624.0
80	3624.0
81	3624.0
82	2468.0
83	1312.0
84	1312.0
85	1312.0
86	932.5
87	553.0
88	553.0
89	553.0
90	418.0
91	283.0
92	283.0
93	283.0
94	163.5
95	44.0
96	44.0
97	44.0
98	31.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30841959432857285
2	0.3112368548385393
3	0.3230693489803982
4	0.32264675990390324
5	0.32976034269156845
6	0.27531678333646753
7	0.28292338671337686
8	0.2813738934328953
9	0.28017655771615957
10	0.3934304302168093
11	0.281233030407397
12	0.2802469892289087
13	0.2736968585432369
14	0.2787679274611764
15	0.27256995433925024
16	0.27771145476993897
17	0.2705978719822738
18	0.2714430501352637
19	0.2734151324922402
20	0.46787653919267175
21	0.27348556400498936
22	0.27658455056595244
23	0.2710908925715179
24	0.2736264270304877
25	0.2681327690360532
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1419819.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.79490011810518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.72334017610018	57.059535047949694
2	7.608186322966291	10.01159718399002
3	2.287718785058833	4.515606868837764
4	1.0145803227965415	2.6701684400077346
5	0.5490680347229889	1.8062938251321679
6	0.3372419757779491	1.3313281267165564
7	0.2569643756704304	1.1834861801803058
8	0.1721410761141811	0.906080392732455
9	0.1406851834052362	0.8330730831220341
>10	0.8111686970949552	9.966809558454209
>50	0.0661239919151714	2.9766119508698687
>100	0.030259473349309868	3.542878004236237
>500	0.0013156095797860917	0.5816137603567184
>1k	0.0010963413164884097	2.070380522108214
>5k	1.0963413164884097E-4	0.5445370553060731
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7549	0.531687489743411	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4856	0.34201542590992234	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4651	0.32757696579634443	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4022	0.28327554427712265	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3596	0.25327171984598035	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3290	0.2317196769447373	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	2064	0.14537064231426683	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2047	0.14417330659753108	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1628	0.11466250275563293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.043151274916029E-5	0.0
5	7.043151274916029E-5	0.0	0.0	7.043151274916029E-5	0.0
6	1.4086302549832057E-4	7.043151274916029E-5	0.0	7.043151274916029E-5	0.0
7	1.4086302549832057E-4	7.043151274916029E-5	0.0	7.043151274916029E-5	0.0
8	1.4086302549832057E-4	7.043151274916029E-5	0.0	7.043151274916029E-5	0.0
9	1.4086302549832057E-4	7.043151274916029E-5	0.0	7.043151274916029E-5	0.0
10	1.4086302549832057E-4	7.043151274916029E-5	0.0	7.043151274916029E-5	0.0
11	1.4086302549832057E-4	7.043151274916029E-5	0.0	7.043151274916029E-5	0.0
12	1.4086302549832057E-4	7.043151274916029E-5	0.0	7.043151274916029E-5	0.0
13	1.4086302549832057E-4	7.043151274916029E-5	0.0	7.043151274916029E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	25	0.0060743466	18.9769	11
ATAACGC	180	0.0	15.294511	3
TAACGCA	200	0.0	14.734679	4
TATAACG	190	0.0	13.989897	2
GGTATCA	1640	0.0	13.949799	1
GCGCCGA	70	7.217581E-6	13.57939	19
TAGGAGT	135	1.8189894E-12	13.37916	4
GTATAAC	280	0.0	12.883098	1
TATCACG	90	5.451311E-7	12.657527	2
TTGACCG	130	1.9645086E-10	12.430804	18
TGCGTAG	85	3.9075476E-6	12.303942	14
CTAATCG	70	1.08266264E-4	12.224046	14
GTTATCA	350	0.0	12.205041	1
GAATGCG	70	1.10322384E-4	12.199436	11
ACCGAGT	120	9.995347E-9	11.876083	8
GTGGTAT	445	0.0	11.732685	1
GGACCGA	130	2.6084308E-9	11.693788	6
TGACCGA	140	6.730261E-10	11.54248	19
GTATCAA	4115	0.0	11.188366	1
GTTCGCG	85	5.2844225E-5	11.184215	16
>>END_MODULE