FastQCFastQC Report
Thu 26 May 2016
SRR1364327_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364327_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419819
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT166391.1719099406332778No Hit
GTACTTTTTTTTTTTTTTTTTTTTT131670.9273717283681935No Hit
TATCAACGCAGAGTACTTTTTTTTT127360.8970157463733053No Hit
GGTATCAACGCAGAGTACTTTTTTT115030.8101736911535907No Hit
GAGTACTTTTTTTTTTTTTTTTTTT64510.454353688744833No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52000.36624386629563344No Hit
GTGGTATCAACGCAGAGTACTTTTT23660.1666409591645132No Hit
GTGGTATCAACGCAGAGTACATGGG22050.1553014856118984No Hit
ATCAACGCAGAGTACTTTTTTTTTT19260.1356510935548827No Hit
GTATAACGCAGAGTACTTTTTTTTT18840.13269297001941796No Hit
GCAGAGTACTTTTTTTTTTTTTTTT17980.12663585992299017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA14200.10001274810380761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG402.7509985E-416.6328355
CGTCGCT350.00217551216.27961514
TATAACG3150.014.4763792
TAACGCA3450.014.321554
TGCGTAG801.2940473E-714.24466314
GTATAAC3550.014.1857821
ATAACGC3150.014.1772853
CCGCCGA551.9662008E-413.81300715
GGTATCA32800.013.6153791
GACTTCG500.001503673513.29501917
CGAGTGG1650.013.24586310
ATCACGC1153.765308E-1013.2198923
GGACCGA1800.013.1983376
CCGAGTG1800.013.1978719
AGGACCG1900.013.0061265
GTGCTAT751.4754953E-512.6690641
ATGCGTA905.4261363E-712.66236913
GTGGTAT8900.012.5978891
TGGTATC8950.012.4191562
ACCGAGT1850.012.3275268
Produced by FastQC (version 0.11.5)