##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364327_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1419819
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.49295156636163	31.0	30.0	34.0	26.0	34.0
2	29.523792821479358	31.0	30.0	34.0	25.0	34.0
3	29.557097066597926	31.0	30.0	34.0	25.0	34.0
4	32.88038193600734	35.0	33.0	37.0	26.0	37.0
5	32.45831193976134	35.0	32.0	37.0	25.0	37.0
6	32.33360801623306	35.0	32.0	37.0	23.0	37.0
7	32.054009701236566	35.0	32.0	37.0	19.0	37.0
8	31.933340094758556	35.0	32.0	37.0	18.0	37.0
9	32.61971209006219	37.0	32.0	39.0	13.0	39.0
10	32.755508272533326	37.0	32.0	39.0	17.0	39.0
11	32.686207185563795	37.0	32.0	39.0	15.0	39.0
12	32.62967110596492	37.0	32.0	39.0	15.0	39.0
13	32.67566429242037	37.0	32.0	39.0	15.0	39.0
14	33.564663524012566	38.0	32.0	41.0	16.0	41.0
15	33.594325755606874	38.0	32.0	41.0	16.0	41.0
16	33.55822185785653	38.0	32.0	41.0	13.0	41.0
17	33.550525102143304	38.0	32.0	41.0	11.0	41.0
18	33.510911602112664	38.0	32.0	41.0	11.0	41.0
19	33.41938162540436	39.0	32.0	41.0	10.0	41.0
20	33.30814772868936	39.0	32.0	41.0	10.0	41.0
21	33.2458066838097	38.0	32.0	41.0	2.0	41.0
22	33.18029199496556	38.0	32.0	41.0	2.0	41.0
23	32.908948957578396	38.0	32.0	41.0	2.0	41.0
24	32.81584061066939	38.0	32.0	41.0	2.0	41.0
25	32.65921712556319	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2564528370480552
1101	2	0.44367033954457114
1101	3	-1.5835742366060437
1101	4	-0.44684894857743984
1101	5	0.4028582901356259
1101	6	0.6557034343072843
1101	7	0.8214377219393469
1101	8	0.92115659098598
1101	9	1.7874442520785863
1101	10	1.446375064753255
1101	11	1.4315424187572958
1101	12	1.5838523059441698
1101	13	1.3937047619549574
1101	14	1.5021239805305484
1101	15	1.4109030681110113
1101	16	1.4983690579087252
1101	17	1.3612643890668323
1101	18	1.2420097818994336
1101	19	1.4507169738857968
1101	20	1.6584782270300522
1101	21	1.5354071164319407
1101	22	1.6501595124685835
1101	23	1.832927231145561
1101	24	1.9092770076739427
1101	25	2.0345581928982597
1102	1	0.5514696860778159
1102	2	0.5724883108262695
1102	3	0.6449886263383888
1102	4	0.6361396216138715
1102	5	0.6521061389320124
1102	6	0.9498953400707038
1102	7	0.976718091279551
1102	8	1.0709107835780216
1102	9	1.5058805401108089
1102	10	1.0251298559358517
1102	11	1.1884165279199053
1102	12	1.2997112231875718
1102	13	1.100463015852469
1102	14	1.1233576309115278
1102	15	1.2275485409664455
1102	16	1.2127428364719606
1102	17	1.2504685485788798
1102	18	1.2359026733205596
1102	19	1.2938386908433657
1102	20	1.4422545969706562
1102	21	1.5403973108243747
1102	22	1.5895589914961405
1102	23	1.8931466849171343
1102	24	1.8481986978624931
1102	25	1.8255720303131966
1103	1	-2.0553580031977106
1103	2	-1.9655821588628868
1103	3	-1.7207136884975753
1103	4	-1.645680399400426
1103	5	-3.851951187031087
1103	6	-8.796323717679456
1103	7	-8.690356679162075
1103	8	-9.212938879344794
1103	9	-9.89352949323797
1103	10	-7.617221135064547
1103	11	-11.470466581674234
1103	12	-10.781992631826817
1103	13	-11.57126753123718
1103	14	-11.426564381240222
1103	15	-11.887276981617397
1103	16	-6.306968184287818
1103	17	-3.764016960038944
1103	18	-3.0542016802939607
1103	19	-2.870690216869928
1103	20	-7.502706213319826
1103	21	-4.2918119968132125
1103	22	-10.426396212805912
1103	23	-10.594357638676964
1103	24	-12.967418450327468
1103	25	-12.879806194435382
1104	1	0.6491183012197546
1104	2	0.8787180222290232
1104	3	0.9650022783174954
1104	4	1.1310077760168369
1104	5	1.0489830380328158
1104	6	1.254059059629789
1104	7	0.9773612358620198
1104	8	1.0272334521645448
1104	9	1.4723075501914025
1104	10	1.4103100656990435
1104	11	1.371375456492359
1104	12	1.2675271804129054
1104	13	1.0326668049661691
1104	14	1.2205548190788136
1104	15	1.2847106492166063
1104	16	0.9094281520332075
1104	17	0.8040193367948874
1104	18	0.9102964597548109
1104	19	1.3855607229121105
1104	20	1.6682105563142642
1104	21	1.2981579955377782
1104	22	1.3288891213642344
1104	23	1.6333160820753818
1104	24	1.9257621643390124
1104	25	1.8949638449864352
1105	1	0.4891503965077959
1105	2	0.6109388236684019
1105	3	0.1366586426716907
1105	4	0.5529207651802608
1105	5	0.5758715825291816
1105	6	0.6950891730239732
1105	7	0.8854280742011511
1105	8	0.6945907371467399
1105	9	0.9299963751778009
1105	10	0.7559466582558443
1105	11	1.0993614084201369
1105	12	1.1104576727043352
1105	13	1.097168316430313
1105	14	1.0260893902610135
1105	15	0.7834411764710865
1105	16	1.0437245404294018
1105	17	1.0470063985046636
1105	18	1.1796849404141554
1105	19	1.0298117575628396
1105	20	1.4532271244755037
1105	21	1.387051533071002
1105	22	1.1451384903598694
1105	23	1.6015529429440818
1105	24	1.5985811480929826
1105	25	1.6843193661027343
1106	1	0.015427248132624527
1106	2	0.2359609615045457
1106	3	0.09276838149588329
1106	4	-1.387356114813418
1106	5	-0.3247152474339998
1106	6	-6.212539475072276E-4
1106	7	0.26282904157900333
1106	8	0.46664782338196886
1106	9	1.1145691909281865
1106	10	0.7191103120829041
1106	11	0.6759275210161064
1106	12	0.7555136910549294
1106	13	0.49454034757931
1106	14	0.03034897336047493
1106	15	0.6578559169264864
1106	16	0.5113658122455575
1106	17	0.4031998254416962
1106	18	0.3836020709453223
1106	19	-1.012792178817982
1106	20	0.1419215291247511
1106	21	-0.13587860457872125
1106	22	0.21494430556922595
1106	23	0.11701680406638815
1106	24	0.3135994156072073
1106	25	0.5737862140598722
1107	1	0.2753265329398644
1107	2	0.13095841490212834
1107	3	0.045540537240341905
1107	4	0.26668481125177124
1107	5	0.3338102475150677
1107	6	0.30995643037169174
1107	7	0.30122792244938523
1107	8	0.0711442858498259
1107	9	0.9192312331212804
1107	10	0.728511704764955
1107	11	0.7907942582621601
1107	12	0.5595325088084095
1107	13	0.4856159118873933
1107	14	0.560775107529004
1107	15	0.6752385517275172
1107	16	0.4439678250121446
1107	17	0.2690553685597834
1107	18	0.35720680993172493
1107	19	0.5788962286998967
1107	20	0.4756526637362555
1107	21	0.4571704855823313
1107	22	0.5475050953355662
1107	23	0.6567721198713841
1107	24	0.9570955062994244
1107	25	0.5178661421805586
1108	1	0.06395220990496497
1108	2	0.12597559168964878
1108	3	0.11328451063473466
1108	4	0.014180715500387464
1108	5	0.29914979798896724
1108	6	0.24744691726283463
1108	7	-0.18560347989333792
1108	8	0.377718437362347
1108	9	0.5913391252782247
1108	10	0.16889990507297625
1108	11	0.3738402206941771
1108	12	0.5819163424971734
1108	13	0.1959730483147908
1108	14	0.3487023381791374
1108	15	0.11141602501307091
1108	16	-0.5538562303463763
1108	17	-0.5309655807380054
1108	18	-0.427093164372657
1108	19	-0.31839411056416367
1108	20	0.20458184365308085
1108	21	0.17462910662753472
1108	22	0.16487640036224605
1108	23	-0.09052943054031459
1108	24	0.2689781771384929
1108	25	0.05657406150611877
1109	1	0.40534827371021365
1109	2	0.3474980042981244
1109	3	0.2921142804406145
1109	4	0.31140665751295415
1109	5	0.4995290400102519
1109	6	0.7245112514783187
1109	7	0.8087070747389475
1109	8	0.8208578081938853
1109	9	1.656964581195922
1109	10	0.9283980716215936
1109	11	0.9840685891058385
1109	12	1.0615311669235226
1109	13	1.073460677917673
1109	14	0.9021864255816681
1109	15	0.4948298715443471
1109	16	0.8288187748088092
1109	17	0.7624261698311017
1109	18	0.6197863820692078
1109	19	1.0697706531003277
1109	20	1.3617517021851242
1109	21	1.1751026497488937
1109	22	0.8863924963456071
1109	23	1.122247920242657
1109	24	0.8860280931477575
1109	25	0.8691015184491491
1110	1	0.05407425756502349
1110	2	-0.014599332190645242
1110	3	-0.08555280290800837
1110	4	0.1056396204652188
1110	5	0.005018779249589045
1110	6	0.1584348885886797
1110	7	0.11883771663477205
1110	8	-0.03177595491266416
1110	9	-3.6896034143504473
1110	10	-1.7271578786670254
1110	11	-0.6276861754740608
1110	12	-0.12021308821388743
1110	13	-0.5629932284951984
1110	14	-0.1006240304547461
1110	15	-0.050964672758887275
1110	16	-0.4085796540841926
1110	17	-0.20221650522021406
1110	18	-0.18661012842110125
1110	19	-0.33453212815413735
1110	20	0.09785577533584444
1110	21	-0.12489352437679457
1110	22	0.3988488403869681
1110	23	0.47167341446363764
1110	24	0.5503913622558514
1110	25	0.4828908283316977
1111	1	0.14756344639270225
1111	2	0.09694269466697492
1111	3	0.11042944132401189
1111	4	-0.06385595148017131
1111	5	0.241187913204854
1111	6	0.1974754923096711
1111	7	0.4515616156869484
1111	8	0.5268347215671696
1111	9	1.0828510375008733
1111	10	0.5147862348355119
1111	11	0.7788176366656856
1111	12	0.8454854307908235
1111	13	0.6019887451075618
1111	14	0.5659132837209384
1111	15	0.5694155769043832
1111	16	0.5112153209214796
1111	17	0.5231678972023843
1111	18	0.4584199363121755
1111	19	0.5895812499522677
1111	20	0.7576421337547501
1111	21	0.81225205239015
1111	22	0.6444184527649881
1111	23	0.9321382124404494
1111	24	0.8723670802788277
1111	25	1.092751532029986
1112	1	-0.19456404878206257
1112	2	-0.329960627530383
1112	3	-0.2654386197977523
1112	4	-0.05534686849897952
1112	5	-0.10590748855595677
1112	6	-0.07347900621871162
1112	7	0.14348808618088782
1112	8	-0.38382631940082845
1112	9	0.14013746080050282
1112	10	-0.13416439709374828
1112	11	0.10486075277266593
1112	12	-0.4860297566719076
1112	13	-0.19221424172804547
1112	14	-1.1041938332581083
1112	15	-0.4058474864937338
1112	16	-0.47635769880907475
1112	17	-0.42826476433874205
1112	18	-0.3313906452166435
1112	19	-0.10569883802408242
1112	20	0.04558033229146474
1112	21	-0.3256109622747161
1112	22	-0.2030584790918155
1112	23	0.06510946623170355
1112	24	0.027406163067652756
1112	25	-0.08486034605992643
1113	1	-2.3560756756512653
1113	2	-2.268997098995353
1113	3	-2.2506400763358876
1113	4	-2.319130208451586
1113	5	-2.317176359069535
1113	6	-2.1602002558784505
1113	7	-2.7432614999284546
1113	8	-2.8108209153944586
1113	9	-11.872508516539263
1113	10	-4.805040997433789
1113	11	-3.2653672329297017
1113	12	-13.720615955259102
1113	13	-5.347309447736265
1113	14	-4.266647033491942
1113	15	-3.747539328719956
1113	16	-3.416692738279295
1113	17	-3.6425993263286323
1113	18	-3.9175524173380367
1113	19	-5.029626069129026
1113	20	-16.256967047590994
1113	21	-18.459548099980935
1113	22	-13.244052062113148
1113	23	-18.725409425478432
1113	24	-19.447107766416984
1113	25	-16.381165897772835
1114	1	-0.20125518396056563
1114	2	-0.1781194722957231
1114	3	-0.2072476851808247
1114	4	-0.6836372816558125
1114	5	-0.2391618006297591
1114	6	-0.12265870938758638
1114	7	-0.20680112360651748
1114	8	-0.040474534402836326
1114	9	0.4512200921683345
1114	10	-0.24958597751894018
1114	11	-0.21472528814189928
1114	12	0.05186474794101059
1114	13	-0.17884962078941413
1114	14	-0.4030486266796913
1114	15	-0.37372474281142587
1114	16	-0.31995657566427127
1114	17	-0.4777549368216967
1114	18	-0.38212871180308383
1114	19	-0.5945111123923112
1114	20	-0.8221091983944504
1114	21	-0.9439579337949695
1114	22	-1.0297792973830298
1114	23	-0.7340922987101308
1114	24	-0.38426090472216146
1114	25	-0.6873617499909344
1115	1	-0.11309949418969012
1115	2	-0.07071653873230588
1115	3	-0.11755733909703636
1115	4	0.004635750651729609
1115	5	-0.2407075776405918
1115	6	-0.17139229197793782
1115	7	-0.28034805103437677
1115	8	-0.1052997668829434
1115	9	0.16637031745833042
1115	10	-0.12644035389704555
1115	11	0.03143289419920592
1115	12	0.140280815432142
1115	13	-0.20247688567293665
1115	14	-0.34072094956340493
1115	15	-0.4132553726647501
1115	16	-0.2556037811095777
1115	17	-0.14054201380376696
1115	18	-0.2642991652591249
1115	19	-0.16673544371241888
1115	20	-0.7490289568068746
1115	21	-0.22329951178365803
1115	22	-0.5470470569458712
1115	23	-0.49123734712351563
1115	24	-0.13857861155755558
1115	25	-0.11630317459068351
1116	1	-1.013595053241609
1116	2	-0.9305900605310171
1116	3	-0.9785679466187318
1116	4	-0.8423300921133325
1116	5	-2.078325199322574
1116	6	-1.8475475982764742
1116	7	-3.084239032103252
1116	8	-2.315899371004143
1116	9	-1.2636295810342837
1116	10	-2.459651336996064
1116	11	-2.1239801901026816
1116	12	-3.0380698925818663
1116	13	-2.6924966796129475
1116	14	-2.087466927333285
1116	15	-3.2810188604584845
1116	16	-3.7700480789322413
1116	17	-4.3595850021811735
1116	18	-3.527880345790013
1116	19	-2.9290993476864315
1116	20	-3.161403316041511
1116	21	-2.4658664305269937
1116	22	-2.3259361601281263
1116	23	-2.522301449036931
1116	24	-3.1260909700816377
1116	25	-2.7828650179647916
1201	1	0.8648188901135114
1201	2	0.9457181002165456
1201	3	0.5819803380231008
1201	4	1.0009392466797493
1201	5	1.1821394041895132
1201	6	1.232209652950658
1201	7	1.4220038741623355
1201	8	1.191737870773423
1201	9	2.0246480261263855
1201	10	1.5803345936546407
1201	11	1.7912702495644837
1201	12	1.7218549308950486
1201	13	1.4701838464127235
1201	14	1.5146399052257067
1201	15	1.502319880124766
1201	16	1.522035392207215
1201	17	1.5765279725937447
1201	18	1.3543193024762559
1201	19	1.5551577854211587
1201	20	1.8449144517617242
1201	21	1.8989996744156485
1201	22	1.7523179806178675
1201	23	2.0603833817896486
1201	24	1.9639461457017546
1201	25	2.178471360227718
1202	1	1.1454625324406251
1202	2	1.1477551441005929
1202	3	1.0368723401491948
1202	4	1.3142088871785162
1202	5	1.4311964243203121
1202	6	1.3850591531483403
1202	7	1.3928524396454662
1202	8	1.499638086767213
1202	9	2.144209911448371
1202	10	1.6799082988705578
1202	11	1.7224116401059462
1202	12	1.997118389131522
1202	13	1.731472167899753
1202	14	1.880416715032034
1202	15	1.6566373790649465
1202	16	1.6295770596808552
1202	17	1.6595587170338248
1202	18	1.7238296885086726
1202	19	1.9407488613034332
1202	20	2.0817925990628012
1202	21	1.9358166290186105
1202	22	2.0826918410065645
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2211	6	0.062340099966135654
2211	7	0.10192793292536706
2211	8	0.11987199129131199
2211	9	0.6350884543153015
2211	10	-0.1284122860392074
2211	11	0.2767169178875335
2211	12	0.29327142508536497
2211	13	0.26482461838760685
2211	14	0.2730089053486964
2211	15	0.2752285362284326
2211	16	0.1561099100241634
2211	17	0.09628973622683645
2211	18	0.13501468963522
2211	19	-0.11791274119642736
2211	20	0.3842345650003196
2211	21	0.48140549907639496
2211	22	0.44350545758196347
2211	23	0.7573655285985339
2211	24	0.9243281318068668
2211	25	0.9910896478277991
2212	1	-0.23693815703602894
2212	2	-0.11127646888275322
2212	3	-0.1751581895418397
2212	4	-0.026583053366088905
2212	5	-0.3009642276301392
2212	6	-0.09736671090445981
2212	7	-0.031141662097226686
2212	8	-0.20887005962088523
2212	9	0.2743198267151641
2212	10	-0.16545097562284639
2212	11	0.1481404683996317
2212	12	-0.1166559194840957
2212	13	-0.1643206105505115
2212	14	-0.29221055311123934
2212	15	-0.03978958922780862
2212	16	-0.14821671976741868
2212	17	-0.21424109533516145
2212	18	-0.10097209443485866
2212	19	-0.0530981431090396
2212	20	0.11099660365332653
2212	21	0.403435316110226
2212	22	-0.061013654607144474
2212	23	0.23967144797170192
2212	24	0.3719389435903153
2212	25	0.43137127996735103
2213	1	-0.45391053819313854
2213	2	-0.425332587049823
2213	3	-0.4708791682601081
2213	4	-0.5812141199263579
2213	5	-0.48876624890893083
2213	6	-0.29477451803488464
2213	7	-0.7022634953829545
2213	8	-0.5509425623963153
2213	9	0.1353090577516909
2213	10	-0.5103920363819441
2213	11	-0.2886706433331554
2213	12	-0.23029726912351123
2213	13	-0.5894484128515387
2213	14	-0.48653630763070055
2213	15	-0.4973796177035723
2213	16	-0.8548272496169105
2213	17	-0.8696188041402664
2213	18	-1.064558009090014
2213	19	-1.095984796602842
2213	20	-0.5605043666429452
2213	21	-0.7094945074695929
2213	22	-0.6488589637620876
2213	23	-0.915741229780977
2213	24	-0.7235656693660104
2213	25	-0.43726952489267035
2214	1	-1.053524650277275
2214	2	-0.9139374950428802
2214	3	-0.9651829432735646
2214	4	-0.763052854709187
2214	5	-1.1799072074539083
2214	6	-0.9424487356343043
2214	7	-0.9428501174822834
2214	8	-0.8136818834842003
2214	9	-10.751408079310234
2214	10	-4.055538733424452
2214	11	-1.949083993303752
2214	12	-1.2297590490843646
2214	13	-1.1652608193895908
2214	14	-1.3913075943087385
2214	15	-1.3824141566347024
2214	16	-1.4562715362941887
2214	17	-1.351154761843567
2214	18	-1.722251196492497
2214	19	-1.7356449706600294
2214	20	-1.3698782272658434
2214	21	-2.0438144517302277
2214	22	-1.8336813096314977
2214	23	-2.231765089611269
2214	24	-2.5585076238822673
2214	25	-10.330112406158914
2215	1	-0.743922242899469
2215	2	-0.5397611316072854
2215	3	-0.4092463122279959
2215	4	-0.47976241649939766
2215	5	-0.6319330078475716
2215	6	-0.5743190016303998
2215	7	-0.6719100772203284
2215	8	-0.8182134772291576
2215	9	0.07499022063748839
2215	10	-0.0011615389585770686
2215	11	0.004899307327356439
2215	12	-0.04086683978086825
2215	13	-0.17947451046285323
2215	14	-0.3687071536974429
2215	15	-0.8986073553445237
2215	16	-0.5461733060751754
2215	17	-0.5397029607503043
2215	18	-0.44774016615239987
2215	19	-0.7385499473437704
2215	20	-0.4898938409363751
2215	21	-0.552572624390244
2215	22	-0.6037657639908574
2215	23	-0.27389420123048325
2215	24	-0.08972878743276169
2215	25	-0.08739666222002285
2216	1	-1.1188922176321263
2216	2	-1.1723402810190322
2216	3	-1.1152521276059844
2216	4	-0.8075878091938051
2216	5	-2.005547584869248
2216	6	-2.3541827641101847
2216	7	-1.6963539531284546
2216	8	-2.7457089228121454
2216	9	-1.463639416135976
2216	10	-1.7677470154310022
2216	11	-1.0228749170984308
2216	12	-2.341696158717582
2216	13	-2.0736592527748776
2216	14	-2.285604030788935
2216	15	-1.975643870480667
2216	16	-3.1318957952109407
2216	17	-3.4445502318895933
2216	18	-2.9268497981779618
2216	19	-4.425093574909351
2216	20	-3.125244137058697
2216	21	-3.2640986779011847
2216	22	-3.3242757383946895
2216	23	-3.0006085287065964
2216	24	-2.9322810975637985
2216	25	-2.8827139636312005
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54700.0
3	0.0
4	805.0
5	7820.0
6	10617.0
7	8902.0
8	6853.0
9	5410.0
10	4642.0
11	4104.0
12	3664.0
13	3392.0
14	3439.0
15	3372.0
16	3495.0
17	3839.0
18	4404.0
19	5407.0
20	7362.0
21	9581.0
22	11622.0
23	13875.0
24	15860.0
25	17509.0
26	19626.0
27	21149.0
28	23895.0
29	27625.0
30	33192.0
31	41630.0
32	50953.0
33	63432.0
34	79006.0
35	98171.0
36	129250.0
37	192075.0
38	429141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1404585057315	19.02683951453992	13.386786451247925	23.445915528480658
2	16.04075779580533	21.576177523344807	39.12256779466433	23.26049688618554
3	18.47774641221116	27.833043154887676	29.37112978019687	24.318080652704293
4	11.976331327605164	17.839406043385992	36.97430634744997	33.20995628155888
5	12.292767952947907	39.085913892358754	34.00335220799754	14.617965946695808
6	29.18994383894251	38.26383229306591	18.504128080740063	14.042095787251519
7	26.617266243811443	32.984257049387274	22.374038079512186	18.024438627289097
8	26.09816445378163	34.797810927608154	20.332122974692375	18.771901643917843
9	26.373162605251903	15.743733147894117	20.269591420758907	37.613512826095075
10	15.311153120690953	30.580382778828017	31.835972867416874	22.272491233064155
11	33.10791244237358	23.984778100870095	23.511788798615626	19.395520658140693
12	22.903355546805717	27.527260868248355	29.98691985470262	19.58246373024331
13	28.318773555602668	22.543438098464623	26.9008768266047	22.23691151932801
14	21.898992204162475	21.79686025249836	29.385475568628443	26.918671974710723
15	23.392828213815303	29.27486212167102	25.281990237580388	22.05031942693329
16	21.89101579779276	28.717637346692097	26.803489771020754	22.587857084494384
17	21.128612806338364	28.142345555874876	28.385148118720384	22.343893519066377
18	20.60340303533	26.63834912986972	31.22733980691948	21.5309080278808
19	22.154941165862677	26.088206122356972	31.09847673684685	20.658375974933502
20	22.17932353919066	25.439200802466004	31.362952443745666	21.018523214597668
21	22.990511221918787	25.004913089385827	30.85899116489173	21.145584523803652
22	22.4341631195133	25.06263722253489	31.131597687652807	21.371601970299004
23	21.72464799149122	25.27797930563284	31.332455219727972	21.664917483147967
24	21.728650889632842	25.611677606440036	31.271575207625606	21.388096296301516
25	21.537497285666344	25.064086475145164	31.644688789308482	21.753727449880007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1960.0
1	1960.0
2	2412.5
3	2865.0
4	2865.0
5	2865.0
6	9341.5
7	15818.0
8	15818.0
9	15818.0
10	13785.5
11	11753.0
12	11753.0
13	11753.0
14	11205.5
15	10658.0
16	10658.0
17	10658.0
18	17585.0
19	24512.0
20	24512.0
21	24512.0
22	34681.5
23	44851.0
24	44851.0
25	44851.0
26	62307.0
27	79763.0
28	79763.0
29	79763.0
30	92310.5
31	104858.0
32	104858.0
33	104858.0
34	113241.5
35	121625.0
36	121625.0
37	121625.0
38	128321.5
39	135018.0
40	135018.0
41	135018.0
42	143042.5
43	151067.0
44	151067.0
45	151067.0
46	160365.0
47	169663.0
48	169663.0
49	169663.0
50	166710.0
51	163757.0
52	163757.0
53	163757.0
54	150089.5
55	136422.0
56	136422.0
57	136422.0
58	119730.0
59	103038.0
60	103038.0
61	103038.0
62	85674.5
63	68311.0
64	68311.0
65	68311.0
66	53703.5
67	39096.0
68	39096.0
69	39096.0
70	29452.5
71	19809.0
72	19809.0
73	19809.0
74	14047.0
75	8285.0
76	8285.0
77	8285.0
78	6246.5
79	4208.0
80	4208.0
81	4208.0
82	2872.5
83	1537.0
84	1537.0
85	1537.0
86	1068.0
87	599.0
88	599.0
89	599.0
90	459.0
91	319.0
92	319.0
93	319.0
94	172.0
95	25.0
96	25.0
97	25.0
98	13.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026904837870179228
2	0.0011973357167357247
3	2.1129453824748085E-4
4	0.0036624386629563344
5	0.03211676981361709
6	0.010705589937872361
7	0.018523487853029152
8	0.028524762663409912
9	0.0342297151960919
10	0.03986423621602472
11	0.03859646898653984
12	0.007747466402407631
13	0.002817260509966411
14	0.006127541609176945
15	0.006268404634675265
16	0.008944802119143355
17	0.01035343237412656
18	0.014368028600828697
19	0.016551405496052666
20	0.013734144986086253
21	0.01000127481038076
22	0.009578685733885798
23	0.00767703488965847
24	0.0053527949689361806
25	0.10022404264205508
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1419819.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.52028743411561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.03038635004707	40.71668220783632
2	11.526142202265294	11.876603185343967
3	3.7810015456152306	5.843948592567962
4	1.617072931651034	3.3324824896235707
5	0.9199059346226538	2.3696909082053943
6	0.5890439055314665	1.8208626794577114
7	0.3890672326368719	1.4031398959653296
8	0.3091527170389716	1.2742109474308503
9	0.2197933317943242	1.0191434067130973
>10	1.4284879514494575	13.89156287707774
>50	0.12446621010440226	4.408768839983499
>100	0.06094966790506308	5.495807630946207
>500	0.0027454662656897656	0.998970928739846
>1k	9.60913192991418E-4	0.9018674182860542
>5k	2.745466265689766E-4	0.8240007285485271
>10k+	5.490932531379532E-4	3.822257263273981
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	16639	1.1719099406332778	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	13167	0.9273717283681935	No Hit
TATCAACGCAGAGTACTTTTTTTTT	12736	0.8970157463733053	No Hit
GGTATCAACGCAGAGTACTTTTTTT	11503	0.8101736911535907	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	6451	0.454353688744833	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5200	0.36624386629563344	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2366	0.1666409591645132	No Hit
GTGGTATCAACGCAGAGTACATGGG	2205	0.1553014856118984	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1926	0.1356510935548827	No Hit
GTATAACGCAGAGTACTTTTTTTTT	1884	0.13269297001941796	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1798	0.12663585992299017	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1420	0.10001274810380761	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	7.043151274916029E-5
11	0.0	0.0	0.0	0.0	7.043151274916029E-5
12	0.0	0.0	0.0	0.0	7.043151274916029E-5
13	0.0	0.0	0.0	0.0	7.043151274916029E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	40	2.7509985E-4	16.632835	5
CGTCGCT	35	0.002175512	16.279615	14
TATAACG	315	0.0	14.476379	2
TAACGCA	345	0.0	14.32155	4
TGCGTAG	80	1.2940473E-7	14.244663	14
GTATAAC	355	0.0	14.185782	1
ATAACGC	315	0.0	14.177285	3
CCGCCGA	55	1.9662008E-4	13.813007	15
GGTATCA	3280	0.0	13.615379	1
GACTTCG	50	0.0015036735	13.295019	17
CGAGTGG	165	0.0	13.245863	10
ATCACGC	115	3.765308E-10	13.219892	3
GGACCGA	180	0.0	13.198337	6
CCGAGTG	180	0.0	13.197871	9
AGGACCG	190	0.0	13.006126	5
GTGCTAT	75	1.4754953E-5	12.669064	1
ATGCGTA	90	5.4261363E-7	12.662369	13
GTGGTAT	890	0.0	12.597889	1
TGGTATC	895	0.0	12.419156	2
ACCGAGT	185	0.0	12.327526	8
>>END_MODULE