FastQCFastQC Report
Thu 26 May 2016
SRR1364326_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364326_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023894
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54580.5330629928488692No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA48520.4738771786923256No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39920.38988410909723076No Hit
TATCAACGCAGAGTACTTTTTTTTT32820.3205409935012804No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN29020.2834277767034478No Hit
GGTATCAACGCAGAGTACTTTTTTT24460.23889191654604872No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA21780.2127173320675773No Hit
GAACAAAAAAAAAAAAAAAAAAAAA18790.18351509042928274No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16850.16456781659038924No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11850.1157346365932411No Hit
GTGGTATCAACGCAGAGTACATGGG10790.10538200243384568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACG851.035005E-915.62858511
GCCCCCG1250.015.2133518
GTCGTGC953.0013325E-1015.01317417
ACGGCCC1151.8189894E-1214.88262515
CGCGCCA602.571429E-514.24713210
ATAACGC1152.5465852E-1114.0358343
TCGCGGT551.9445748E-413.830317517
TATAACG1101.8735591E-1013.8113382
CGCGGTT655.4041964E-513.165418
CGCATAT604.096833E-412.6641188
TTCAACG905.431393E-712.6603932
GTATAAC1457.2759576E-1212.4421111
AGTCGTG1155.2459654E-912.40218716
GTCTTAC550.00307860412.0849211
TGCACGG1103.8708095E-812.07663412
GGTATCA15250.011.8924671
TAAACGC657.989968E-411.6974114
TAACGCA1302.59206E-911.6974114
GTTTAGG907.4946383E-611.605361
GAACAAA7200.011.605361
Produced by FastQC (version 0.11.5)