##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364326_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1023894
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.546982402475255	30.0	25.0	31.0	2.0	34.0
2	25.28028584990243	30.0	25.0	31.0	2.0	34.0
3	24.946559897801922	30.0	23.0	31.0	2.0	34.0
4	28.527304584263604	35.0	27.0	37.0	2.0	37.0
5	29.221444797996668	35.0	28.0	37.0	2.0	37.0
6	29.262062283791096	35.0	29.0	37.0	2.0	37.0
7	28.878823393827876	35.0	28.0	37.0	2.0	37.0
8	28.45868126974081	35.0	27.0	37.0	2.0	37.0
9	29.43890285517837	35.0	27.0	39.0	2.0	39.0
10	28.750929295415347	35.0	25.0	39.0	2.0	39.0
11	29.39262951047667	35.0	27.0	39.0	2.0	39.0
12	28.935847851437746	35.0	26.0	39.0	2.0	39.0
13	28.52687094562523	35.0	25.0	39.0	2.0	39.0
14	28.787749513133196	35.0	21.0	40.0	2.0	41.0
15	29.485666485007236	36.0	27.0	40.0	2.0	41.0
16	29.80390841239425	36.0	27.0	40.0	2.0	41.0
17	29.956641996144132	36.0	27.0	40.0	2.0	41.0
18	29.76782264570356	36.0	27.0	40.0	2.0	41.0
19	29.75034232059178	37.0	27.0	40.0	2.0	41.0
20	29.66490574219597	37.0	27.0	40.0	2.0	41.0
21	29.554706834887206	37.0	27.0	40.0	2.0	41.0
22	29.61058664275794	37.0	27.0	40.0	2.0	41.0
23	29.60651590887338	37.0	27.0	40.0	2.0	41.0
24	29.511042158660956	37.0	27.0	40.0	2.0	41.0
25	29.294653548121193	37.0	26.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8272787280156386
1101	2	0.8521248289753593
1101	3	1.5858245178427524
1101	4	0.9347191706437528
1101	5	0.620053062642441
1101	6	0.4921561548537632
1101	7	0.8329524740297138
1101	8	1.0636993821355567
1101	9	0.9953212518807923
1101	10	1.3569462594289625
1101	11	0.846554866650262
1101	12	1.2821912009750207
1101	13	1.008061017083076
1101	14	1.4342084577703496
1101	15	0.7651002188114333
1101	16	0.42189340782480045
1101	17	-0.006531590024049905
1101	18	-5.7462284432549
1101	19	-7.46920074707597
1101	20	-8.234183063641009
1101	21	-10.221874277281213
1101	22	-10.450820570740294
1101	23	-11.64557501993307
1101	24	-11.393921165561839
1101	25	-12.058243070979223
1102	1	1.5001365388044867
1102	2	1.7449243683266218
1102	3	2.3936244808151272
1102	4	1.8778605879230987
1102	5	1.1456844934462858
1102	6	1.14224746873818
1102	7	1.4202741216961918
1102	8	1.7122484013742323
1102	9	1.7780789311901941
1102	10	-1.5740701802081674
1102	11	0.5089900290854246
1102	12	1.4909391338608842
1102	13	2.005007022649771
1102	14	2.4905751693094764
1102	15	2.050733814789858
1102	16	1.8643923330823462
1102	17	1.7040206550109538
1102	18	1.7502068635252357
1102	19	1.4442824853727778
1102	20	1.868947496386106
1102	21	1.9718900199313616
1102	22	1.7880319181121926
1102	23	1.7132124509233684
1102	24	1.9545087690749945
1102	25	1.9520171892810367
1103	1	0.04067707934502707
1103	2	-0.4964761230738688
1103	3	-2.459876747686099
1103	4	-0.255124670062159
1103	5	-2.494671771909978
1103	6	-6.6833053568146426
1103	7	-8.376716050293982
1103	8	-3.154459215333386
1103	9	-9.599070945959681
1103	10	-6.71657632271431
1103	11	-9.916071395975997
1103	12	-4.52718125925951
1103	13	-2.13811337047062
1103	14	-1.6304322016978965
1103	15	-1.9062686422125985
1103	16	-1.7302022615122503
1103	17	-1.882895806905509
1103	18	-1.6899037015853295
1103	19	-6.875127834037542
1103	20	-3.419136041697435
1103	21	-7.757532584491244
1103	22	-5.10570272562245
1103	23	-3.771431283720368
1103	24	-8.65574921618299
1103	25	-6.2202801080162615
1104	1	1.6502285652535669
1104	2	1.8646928647537422
1104	3	2.45983758502582
1104	4	2.013476602007149
1104	5	1.7182764680286127
1104	6	1.4898221790993809
1104	7	1.5852452393211074
1104	8	1.8740526829010697
1104	9	2.1884057510465666
1104	10	2.258558012190832
1104	11	1.6524119903602212
1104	12	2.213291928590035
1104	13	2.363586377308742
1104	14	2.7826887545473085
1104	15	1.919260472821943
1104	16	1.0957106675369879
1104	17	0.9189876232951733
1104	18	1.0922681374321073
1104	19	1.280399087774061
1104	20	-8.441287593402663
1104	21	-3.200577326239241
1104	22	-1.0031147225519383
1104	23	0.13338284063537387
1104	24	0.6384579337237959
1104	25	1.0368067436122068
1105	1	1.182254952843099
1105	2	1.1746868785130715
1105	3	1.6863769362857894
1105	4	1.1783087917378872
1105	5	0.9218154192031491
1105	6	0.9565722219656791
1105	7	0.983604738642768
1105	8	1.32508057156301
1105	9	1.1802874761668178
1105	10	1.8839110951047786
1105	11	1.5386440880412309
1105	12	1.6805097405137843
1105	13	1.816092025131283
1105	14	2.3444034364793893
1105	15	1.5599521693947445
1105	16	1.2404393163612504
1105	17	0.590097845327545
1105	18	1.0752005831573612
1105	19	0.8660873099207436
1105	20	0.9968127227550703
1105	21	1.0261049126749313
1105	22	0.8804115341825813
1105	23	0.9645129466128921
1105	24	0.7924201331708964
1105	25	0.8043458271458341
1106	1	0.7311775900702564
1106	2	0.8905968538867484
1106	3	1.3156737006171575
1106	4	0.7634174931429243
1106	5	0.4125322911929601
1106	6	0.01908370231598866
1106	7	0.47950061069796135
1106	8	0.42317101960022185
1106	9	0.8286330603960081
1106	10	-2.4844517057582323
1106	11	-0.6284763443360042
1106	12	0.13294528710299147
1106	13	0.8407199061154635
1106	14	-0.29848917593464463
1106	15	0.7426928831534205
1106	16	0.3239913600971036
1106	17	-6.0217137617346985
1106	18	-2.3976411888172002
1106	19	-0.7020203547502852
1106	20	0.05300105965315183
1106	21	0.27814724753690356
1106	22	0.44576702809449387
1106	23	-0.08734321199184691
1106	24	0.307407633209813
1106	25	0.386617519948782
1107	1	0.7273980366327102
1107	2	1.2554766773249995
1107	3	1.5791446361707493
1107	4	1.0979513368072666
1107	5	0.7296302058197881
1107	6	1.0104096728236023
1107	7	0.9480374259677227
1107	8	1.1982636255064563
1107	9	1.2643715226016141
1107	10	-1.9432258158171862
1107	11	0.15561438320521148
1107	12	0.7500817732594669
1107	13	1.4916578333408026
1107	14	2.117351854587074
1107	15	1.595060924016419
1107	16	-5.217349787965077
1107	17	-1.3753131245056416
1107	18	0.23248979461986607
1107	19	0.8523803372549068
1107	20	1.1968409124879287
1107	21	1.2514073634340797
1107	22	1.1077480527277785
1107	23	0.8596140877271985
1107	24	0.9452631931018125
1107	25	1.3239764428673446
1108	1	-0.427381888713942
1108	2	-2.911709538307285
1108	3	-0.15121581402516782
1108	4	-0.7486750386125287
1108	5	-1.221023123261329
1108	6	-0.49227341578270156
1108	7	-0.27751457609250707
1108	8	0.026130465256294144
1108	9	-0.4977195946083306
1108	10	-3.3007286568666423
1108	11	-1.4074718873764915
1108	12	-5.1079552150334635
1108	13	-1.4870077193649713
1108	14	-5.179572250837943
1108	15	-2.264991000934959
1108	16	-7.131922163232151
1108	17	-3.2124412614509623
1108	18	-1.9537243404059694
1108	19	-0.6539976129073182
1108	20	-0.5241728967342887
1108	21	-0.14604609800475643
1108	22	-0.1953833153030402
1108	23	-0.26406027634935825
1108	24	-0.03689172232549609
1108	25	-0.24577150850766216
1109	1	-8.942778699915813
1109	2	-6.466612402587078
1109	3	-5.314631108069701
1109	4	-5.620843081816968
1109	5	-2.9264829434651354
1109	6	-1.833198800816664
1109	7	-1.2860523185907695
1109	8	-0.9091560915601811
1109	9	-5.847178174942915
1109	10	-1.7277506819762678
1109	11	-1.541442939916326
1109	12	-6.368111380957675
1109	13	-7.980242247146073
1109	14	-8.254443754611358
1109	15	-4.20431698498642
1109	16	-2.406524397118776
1109	17	-2.2633638120650623
1109	18	-1.424992872131007
1109	19	-1.108976188027782
1109	20	-0.9986324244017126
1109	21	-0.8928096300859956
1109	22	-1.02208430825943
1109	23	-1.3420106118567574
1109	24	-1.1465587613571273
1109	25	-1.0033615392315625
1110	1	-2.551218961887564
1110	2	-2.182954903641914
1110	3	-1.6806931749259277
1110	4	-2.3116138684391743
1110	5	-5.098973230403235
1110	6	-4.084363586883729
1110	7	-7.8598693565775655
1110	8	-9.168769995024139
1110	9	-11.015454321329788
1110	10	-8.53577250650639
1110	11	-11.187153751497437
1110	12	-6.296358252350856
1110	13	-4.6285728506767825
1110	14	-4.134078434837495
1110	15	-9.214635787732941
1110	16	-11.031551093621033
1110	17	-11.554474379117373
1110	18	-8.29494537282121
1110	19	-10.968093071453925
1110	20	-12.028139221870699
1110	21	-13.122169606094488
1110	22	-13.849314963806462
1110	23	-14.581333685299487
1110	24	-14.648031812958953
1110	25	-15.003000252768976
1111	1	-0.4073829251665906
1111	2	0.30213655457106725
1111	3	-2.1836811690711677
1111	4	-3.2443813810930635
1111	5	-0.8762700123791873
1111	6	-0.5456446304442402
1111	7	0.02845539116778184
1111	8	0.37497908829846693
1111	9	-4.145145838808769
1111	10	-4.153639259777247
1111	11	-1.1617853594318994
1111	12	-5.470983424835868
1111	13	-0.9002947374906967
1111	14	0.20833387093914624
1111	15	0.2838960318230406
1111	16	0.2482547653318754
1111	17	0.20206358853775797
1111	18	0.4250579496666447
1111	19	0.6504755947916934
1111	20	0.7517414678897545
1111	21	0.9454056063501746
1111	22	0.8476584293516076
1111	23	0.47276000885802105
1111	24	0.7471100213536666
1111	25	1.008317906872076
1112	1	-0.44332495214304046
1112	2	0.16282639935714371
1112	3	-2.127382813935185
1112	4	-0.24733024536829262
1112	5	-0.561393458045071
1112	6	-0.2647200625310262
1112	7	0.17728062032280079
1112	8	-3.4682112609290066
1112	9	-0.8167787502596653
1112	10	-3.2247728127543738
1112	11	-1.0389199936849316
1112	12	0.31005281330975976
1112	13	0.7275826963427647
1112	14	1.5093313556634271
1112	15	0.9447273512414931
1112	16	0.5505098879149841
1112	17	0.33617337654915147
1112	18	0.4636076691048139
1112	19	0.5494390639930451
1112	20	0.47009142853562125
1112	21	0.44280176458612175
1112	22	0.3214740702694314
1112	23	0.16610045828064912
1112	24	0.29332956040975233
1112	25	0.35847532339280264
1113	1	-1.5829897566123137
1113	2	-0.917248088538571
1113	3	-2.131727941185339
1113	4	-1.6837051677767363
1113	5	-2.1982649664249614
1113	6	-1.8860732593032168
1113	7	-1.3323407328739698
1113	8	-10.20626216099108
1113	9	-9.133352307056686
1113	10	-4.278155804684854
1113	11	-9.885599075000886
1113	12	-7.904720619340768
1113	13	-3.2041367325833683
1113	14	-2.2125805003266414
1113	15	-7.700834411638706
1113	16	-3.9249711797448548
1113	17	-2.871151266529683
1113	18	-2.3824506797468317
1113	19	-1.8645894897003146
1113	20	-6.54915127785792
1113	21	-2.951984082713693
1113	22	-2.8212943965213846
1113	23	-2.6886481450713084
1113	24	-6.994690303029106
1113	25	-4.531034297960396
1114	1	-4.273484949102716
1114	2	-4.20859733519508
1114	3	-1.064004762528402
1114	4	-0.5988939959743433
1114	5	-0.5307725758679247
1114	6	-0.8028167977546552
1114	7	-0.6015923644560104
1114	8	-0.08148457093016859
1114	9	-0.3220146689034067
1114	10	-3.2989820122628544
1114	11	-1.4466836408739567
1114	12	-4.665322879543986
1114	13	-1.200574718646255
1114	14	-5.527927563478972
1114	15	-1.975223496881739
1114	16	-1.0279759164287618
1114	17	-1.08566327753012
1114	18	-0.6519476100578103
1114	19	-0.34281490886747434
1114	20	-0.11363202190770139
1114	21	-0.03512998708864501
1114	22	-0.029635935269944014
1114	23	-0.5358097623845595
1114	24	-0.4797288080197255
1114	25	-0.36429680560438626
1115	1	0.7549627936307388
1115	2	0.9291437116368755
1115	3	1.489798576989223
1115	4	0.5670417998315855
1115	5	0.5795608136862427
1115	6	-0.13519437688548308
1115	7	-1.9184811034226712
1115	8	0.142471127051504
1115	9	0.33953590173807413
1115	10	0.9425145863765998
1115	11	0.34425918253639765
1115	12	0.7873110665964518
1115	13	1.015959829057124
1115	14	1.5257071135323308
1115	15	0.6014055018251803
1115	16	0.4244612567876338
1115	17	0.15178542095753755
1115	18	0.35333714847370246
1115	19	0.8583432403426237
1115	20	0.602705752871632
1115	21	0.6998168842218604
1115	22	0.41968098157943956
1115	23	0.294094099986836
1115	24	0.1864880919634082
1115	25	0.313220482302512
1116	1	0.49650130626899625
1116	2	0.7384692384699569
1116	3	1.5224820060920905
1116	4	1.0075177386799936
1116	5	0.09928893490238977
1116	6	-0.972540418428224
1116	7	-4.168247089587169
1116	8	-0.9231176668562853
1116	9	-1.1406891742786769
1116	10	-0.2588920632397702
1116	11	-1.9380430304796015
1116	12	-0.3961942794873643
1116	13	-6.603295886164645
1116	14	-1.3725270633579747
1116	15	-1.0180449732983945
1116	16	-0.6233666418889072
1116	17	-0.2887025960243186
1116	18	-0.09094415729330407
1116	19	0.2803011359694345
1116	20	0.36847149143439495
1116	21	0.5596320283894833
1116	22	0.27107011790231184
1116	23	0.1432397230222513
1116	24	0.4741333692622547
1116	25	0.282597129835402
1201	1	1.7642612355134517
1201	2	2.0085743819766364
1201	3	2.60031789537371
1201	4	2.131082911370143
1201	5	1.4755784834526366
1201	6	1.3517928159239787
1201	7	1.6442946181885958
1201	8	1.9805992343318053
1201	9	2.1931773799965093
1201	10	2.509930316713678
1201	11	1.977016789247017
1201	12	2.267152528051806
1201	13	2.354452740073018
1201	14	2.9499502747519983
1201	15	2.449309612242061
1201	16	2.0043908607213226
1201	17	1.7978867376410825
1201	18	1.6572901470130148
1201	19	1.6718872607303616
1201	20	1.8108038062199405
1201	21	1.668284575876477
1201	22	0.8502614101169677
1201	23	0.4657154646623347
1201	24	0.9478570714120593
1201	25	1.0813740742558977
1202	1	1.9501529634125596
1202	2	2.26497688248077
1202	3	2.769396474554455
1202	4	2.3912964375658454
1202	5	1.9108374949876747
1202	6	1.6858443984512625
1202	7	1.9292084396141753
1202	8	2.173869443303804
1202	9	2.2208707578295694
1202	10	-1.2060032599633885
1202	11	0.7042882806450201
1202	12	1.8289412177794837
1202	13	2.282444293281234
1202	14	2.8900127193822343
1202	15	2.4777438969723526
1202	16	2.004302495678534
1202	17	1.6799307343693073
1202	18	1.8943576964237998
1202	19	2.0585602262385407
1202	20	2.08221137072076
1202	21	2.1955485902323986
1202	22	2.0273336270146274
1202	23	1.7087975014086005
1202	24	1.602919595344261
1202	25	-3.7492426004542523
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1203	2	1.9708448551035609
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2211	17	0.30081520738703915
2211	18	0.8942244000033206
2211	19	1.2671998849997053
2211	20	1.382999870315743
2211	21	1.3824506788847728
2211	22	1.2431097710567904
2211	23	1.11321884114016
2211	24	1.190121088264167
2211	25	1.3039016570334176
2212	1	1.1908747078035233
2212	2	1.2025944890402194
2212	3	1.751866121665465
2212	4	1.3393764516128748
2212	5	1.027401190380374
2212	6	0.869012771590441
2212	7	1.0653707994015633
2212	8	1.4567468523944207
2212	9	1.4054157541791916
2212	10	2.193983446541111
2212	11	1.486716767681731
2212	12	1.5346310471180153
2212	13	1.6579020125012853
2212	14	-4.114007674403801
2212	15	-0.6191198333246604
2212	16	0.1313509913453501
2212	17	0.5947908718681276
2212	18	0.88726487014846
2212	19	1.4068131979034924
2212	20	1.5545847146320178
2212	21	1.4809702464028902
2212	22	1.3514599706706818
2212	23	1.2045862357754125
2212	24	1.2310283716815533
2212	25	1.4352721834055941
2213	1	0.547537627095533
2213	2	0.7945368327015636
2213	3	1.3319356942315714
2213	4	0.8808631394824751
2213	5	0.42765507442468476
2213	6	0.05625349083199538
2213	7	0.11406594281813653
2213	8	-5.131718276176329
2213	9	-1.5690594661586665
2213	10	0.10878037110299843
2213	11	-0.1327663065975493
2213	12	0.5213687325406333
2213	13	-8.139042687695788
2213	14	-2.4701113139266155
2213	15	-1.0441629187118053
2213	16	-0.3215486781743593
2213	17	-0.16329677995531
2213	18	0.17473714464000167
2213	19	0.3373441497814049
2213	20	0.7142483129737514
2213	21	0.7941333532991663
2213	22	0.5186344525331279
2213	23	0.5283555199750367
2213	24	0.6316899682044799
2213	25	0.5776002287245667
2214	1	-0.6051337606463356
2214	2	-0.25138866896028844
2214	3	-6.248502314518319
2214	4	-2.1814094897555307
2214	5	-1.2956056954686055
2214	6	-0.9256936098917343
2214	7	-0.3144994664028147
2214	8	-5.518527451135586
2214	9	-1.5245729973524718
2214	10	-0.04620275329085999
2214	11	-0.20477793883931028
2214	12	0.12524262327126223
2214	13	-5.741006913981742
2214	14	-1.2710605756991917
2214	15	-0.6906564634175218
2214	16	-0.47750306047575464
2214	17	-0.4176885082659041
2214	18	-0.35965645477038777
2214	19	-0.009602029675633617
2214	20	0.20610688639585106
2214	21	0.28746309678709636
2214	22	0.049663286038097
2214	23	0.045238981881013274
2214	24	0.22745635496794847
2214	25	0.4213469703685355
2215	1	0.7821026343299025
2215	2	1.1928179475761347
2215	3	-2.297154896404926
2215	4	0.10636451557956761
2215	5	0.4307334098511362
2215	6	0.0725726321141913
2215	7	0.8340997346743464
2215	8	1.0440227797587802
2215	9	1.2829698004878445
2215	10	1.9283902925912884
2215	11	1.2473709369578607
2215	12	1.162248347542981
2215	13	1.597320059878065
2215	14	1.6529239587769098
2215	15	1.4793673790166437
2215	16	1.1306948451475698
2215	17	0.8316721894421484
2215	18	1.2957223327695182
2215	19	1.0731829574935894
2215	20	1.3656610820827417
2215	21	1.5416385049340846
2215	22	1.476046974771318
2215	23	1.1773788515304453
2215	24	1.134576355922242
2215	25	1.1997606954991191
2216	1	1.013047307304376
2216	2	0.781551891207922
2216	3	1.7710282234756676
2216	4	0.6929459149419621
2216	5	0.3301085082328399
2216	6	0.29454413508318567
2216	7	-0.3815567533975255
2216	8	-4.921501236756072
2216	9	-0.779570808634773
2216	10	0.028617305409831317
2216	11	-0.48520075604191604
2216	12	0.4862834482384315
2216	13	1.2749599610317155
2216	14	2.1104494242290492
2216	15	1.6683262493007227
2216	16	1.0246178428546848
2216	17	0.7977207819437098
2216	18	1.1214599347783079
2216	19	1.381656016552956
2216	20	1.3304382510792756
2216	21	1.0945753528885973
2216	22	0.945162421315203
2216	23	0.6432899467108335
2216	24	0.900423538554719
2216	25	1.0406715536151587
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110705.0
3	5.0
4	681.0
5	4893.0
6	7658.0
7	6648.0
8	5830.0
9	4891.0
10	4447.0
11	4070.0
12	3845.0
13	3761.0
14	3794.0
15	3760.0
16	3715.0
17	3950.0
18	4504.0
19	5800.0
20	8185.0
21	10141.0
22	12596.0
23	14294.0
24	16333.0
25	17947.0
26	19610.0
27	21468.0
28	22931.0
29	26501.0
30	30038.0
31	33738.0
32	42024.0
33	52762.0
34	65928.0
35	81241.0
36	102278.0
37	119363.0
38	143559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.56194487756068	17.822229496179375	12.19453871732477	22.421286908935173
2	14.661823983629485	22.342853726732727	40.149514245308815	22.845808044328976
3	16.06072575689114	30.06707120446867	30.057857857661634	23.814345180978556
4	10.532243251270058	17.87481045922043	36.858293579186636	34.73465271032288
5	11.95425125541448	40.45801676369194	32.85093527521097	14.73679670568262
6	28.718784196434605	38.02442696939624	18.81691724866937	14.43987158549978
7	26.893917868990908	34.396891429288054	21.522698013642128	17.186492688078914
8	25.93063231561013	35.3302758855152	20.56037316408619	18.178718634788478
9	27.435648482437742	18.212412668771794	19.21723003126338	35.13470881752709
10	13.499830325285064	32.311067695307365	32.096081004474314	22.093020974933257
11	32.871845868215956	26.2831333859124	22.294047624879372	18.550973120992275
12	22.423666459289596	29.18040335656936	29.2795524574183	19.116377726722742
13	26.797699368021767	24.857386092560173	26.26162978086	22.083284758558058
14	20.760508351033398	24.054875420294852	28.575445375385026	26.609170853286724
15	22.722508062525225	31.14554474456588	24.25428989039668	21.877657302512215
16	21.952445332706326	31.34238576690963	24.880182616192492	21.82498628419155
17	21.353811550247258	30.670988800852673	25.938780868188623	22.036418780711447
18	20.75012735608762	29.310905599749205	28.688722128610056	21.250244915553118
19	22.031711510484783	28.760978285893422	28.689953123392748	20.517357080229043
20	22.47595599286509	28.18604568974911	28.477803530741085	20.86019478664472
21	23.36074125815209	27.656695903310407	28.09891932508516	20.88364351345235
22	22.79572547083304	27.84429788468019	27.887991504236126	21.47198514025064
23	22.27039884167123	28.285597008565013	28.030106359738276	21.41389779002548
24	22.111987805093097	28.35795892056264	28.14086449820427	21.389188776139992
25	21.72797589426705	27.99117585978551	28.755451414331002	21.52539683161644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8385.0
1	8385.0
2	7802.5
3	7220.0
4	7220.0
5	7220.0
6	10239.0
7	13258.0
8	13258.0
9	13258.0
10	12122.5
11	10987.0
12	10987.0
13	10987.0
14	11061.5
15	11136.0
16	11136.0
17	11136.0
18	14469.0
19	17802.0
20	17802.0
21	17802.0
22	23957.5
23	30113.0
24	30113.0
25	30113.0
26	40106.5
27	50100.0
28	50100.0
29	50100.0
30	59646.5
31	69193.0
32	69193.0
33	69193.0
34	77262.5
35	85332.0
36	85332.0
37	85332.0
38	92608.5
39	99885.0
40	99885.0
41	99885.0
42	107699.0
43	115513.0
44	115513.0
45	115513.0
46	119839.5
47	124166.0
48	124166.0
49	124166.0
50	119785.5
51	115405.0
52	115405.0
53	115405.0
54	105580.5
55	95756.0
56	95756.0
57	95756.0
58	83673.5
59	71591.0
60	71591.0
61	71591.0
62	58709.0
63	45827.0
64	45827.0
65	45827.0
66	36004.5
67	26182.0
68	26182.0
69	26182.0
70	20022.5
71	13863.0
72	13863.0
73	13863.0
74	10192.0
75	6521.0
76	6521.0
77	6521.0
78	4978.5
79	3436.0
80	3436.0
81	3436.0
82	2376.0
83	1316.0
84	1316.0
85	1316.0
86	959.0
87	602.0
88	602.0
89	602.0
90	417.0
91	232.0
92	232.0
93	232.0
94	140.0
95	48.0
96	48.0
97	48.0
98	36.5
99	25.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34290658993997425
2	0.34407858625990584
3	0.355017218579267
4	0.35794720937909585
5	0.3626351946588221
6	0.30637937130210746
7	0.3156576755015656
8	0.31419268010165113
9	0.3136066819416854
10	0.41957468253549685
11	0.3134113492216968
12	0.3134113492216968
13	0.30579337314214167
14	0.3107743575018508
15	0.304914375902193
16	0.30999302662189643
17	0.30296104870230706
18	0.30608637222212454
19	0.30559804042215305
20	0.5118693927301068
21	0.30696536946207326
22	0.30764903398203325
23	0.30403537866224434
24	0.30667237038209033
25	0.29866372886255804
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1023894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.62546815771915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.41756212936916	59.79279032362809
2	6.594992875378717	8.919789613886161
3	2.0319554206372303	4.122358097886235
4	0.86660514738346	2.344183151987828
5	0.4817342535781966	1.6288752212917468
6	0.30370725713902624	1.2323007268154065
7	0.20272602765220865	0.9596609769414729
8	0.15593913510035975	0.8436365612217316
9	0.11872365647721482	0.7225868565601091
>10	0.7294039802290913	9.4136723966609
>50	0.06555136415879174	3.054102527951566
>100	0.028205846553909845	3.4014915924056095
>500	0.0013018078541840528	0.5382873520500331
>1k	0.0014464531713156141	2.4923790169425484
>5k	1.4464531713156143E-4	0.5338855837705035
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5458	0.5330629928488692	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4852	0.4738771786923256	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3992	0.38988410909723076	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3282	0.3205409935012804	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	2902	0.2834277767034478	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2446	0.23889191654604872	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	2178	0.2127173320675773	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	1879	0.18351509042928274	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1685	0.16456781659038924	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1185	0.1157346365932411	No Hit
GTGGTATCAACGCAGAGTACATGGG	1079	0.10538200243384568	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	9.766635999429628E-5	0.0	0.0	0.0	0.0
6	9.766635999429628E-5	0.0	0.0	0.0	0.0
7	9.766635999429628E-5	0.0	0.0	0.0	0.0
8	9.766635999429628E-5	0.0	0.0	0.0	0.0
9	9.766635999429628E-5	0.0	0.0	0.0	0.0
10	1.9533271998859256E-4	0.0	0.0	9.766635999429628E-5	0.0
11	1.9533271998859256E-4	0.0	0.0	9.766635999429628E-5	0.0
12	2.9299907998288886E-4	0.0	0.0	9.766635999429628E-5	0.0
13	2.9299907998288886E-4	0.0	0.0	9.766635999429628E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCACG	85	1.035005E-9	15.628585	11
GCCCCCG	125	0.0	15.21335	18
GTCGTGC	95	3.0013325E-10	15.013174	17
ACGGCCC	115	1.8189894E-12	14.882625	15
CGCGCCA	60	2.571429E-5	14.247132	10
ATAACGC	115	2.5465852E-11	14.035834	3
TCGCGGT	55	1.9445748E-4	13.8303175	17
TATAACG	110	1.8735591E-10	13.811338	2
CGCGGTT	65	5.4041964E-5	13.1654	18
CGCATAT	60	4.096833E-4	12.664118	8
TTCAACG	90	5.431393E-7	12.660393	2
GTATAAC	145	7.2759576E-12	12.442111	1
AGTCGTG	115	5.2459654E-9	12.402187	16
GTCTTAC	55	0.003078604	12.084921	1
TGCACGG	110	3.8708095E-8	12.076634	12
GGTATCA	1525	0.0	11.892467	1
TAAACGC	65	7.989968E-4	11.697411	4
TAACGCA	130	2.59206E-9	11.697411	4
GTTTAGG	90	7.4946383E-6	11.60536	1
GAACAAA	720	0.0	11.60536	1
>>END_MODULE