FastQCFastQC Report
Thu 26 May 2016
SRR1364326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364326_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023894
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT143141.3979962769583572No Hit
GTATCAACGCAGAGTACTTTTTTTT136311.3312901530822527No Hit
TATCAACGCAGAGTACTTTTTTTTT103501.0108468259409666No Hit
GGTATCAACGCAGAGTACTTTTTTT99660.9733429437031568No Hit
GAGTACTTTTTTTTTTTTTTTTTTT66020.6447933086823441No Hit
ACGCAGAGTACTTTTTTTTTTTTTT41490.40521772761633534No Hit
GTGGTATCAACGCAGAGTACTTTTT22300.21779598278728074No Hit
GTGGTATCAACGCAGAGTACATGGG17410.17003713275006982No Hit
ATCAACGCAGAGTACTTTTTTTTTT16580.16193082487054325No Hit
GCAGAGTACTTTTTTTTTTTTTTTT14670.14327655011163265No Hit
GTATAACGCAGAGTACTTTTTTTTT13910.13585390675206613No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11810.1153439711532639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCG402.7670618E-416.619417
TACGGTG350.002163078216.2929635
GAACGGC350.00216661316.2889826
GGCGTAG551.1259364E-515.54781410
TATCACG755.8313162E-815.2015642
TGAACCG400.00526191114.2563445
CGTAAGA400.0052738714.2514672
TATAACG2100.014.0252532
CCAATAC759.63626E-713.936813
TAACGCA2300.013.6345024
TGCTAGC707.24904E-613.57282452
ATAACGC2400.013.4616923
GTGCACG1500.013.29876911
ATCGTCT500.001502746813.29552215
GTCTCGG500.001503300213.29487118
GCCCCCG1550.012.86600518
ACGCCGA751.4743704E-512.6692087
TTACCGC604.1015423E-412.66240116
GTGCTAG701.0901462E-412.2143481
CGCGGGC701.0936326E-412.21017213