##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364326_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1023894
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.45218255014679	31.0	30.0	34.0	26.0	34.0
2	29.461099488814273	31.0	30.0	34.0	25.0	34.0
3	29.503892981109374	31.0	30.0	34.0	25.0	34.0
4	32.82754953149447	35.0	33.0	37.0	26.0	37.0
5	32.426015778977124	35.0	32.0	37.0	25.0	37.0
6	32.299454826378515	35.0	32.0	37.0	23.0	37.0
7	32.03825591320977	35.0	32.0	37.0	19.0	37.0
8	31.91283277370509	35.0	32.0	37.0	18.0	37.0
9	32.6285045131625	37.0	32.0	39.0	12.0	39.0
10	32.74514256358568	37.0	32.0	39.0	17.0	39.0
11	32.67380510091865	37.0	32.0	39.0	15.0	39.0
12	32.66349543995765	37.0	32.0	39.0	15.0	39.0
13	32.662295120393324	37.0	32.0	39.0	15.0	39.0
14	33.547814519862406	38.0	32.0	41.0	13.0	41.0
15	33.571869744329	38.0	32.0	41.0	13.0	41.0
16	33.519125026614084	38.0	32.0	41.0	11.0	41.0
17	33.51761901134297	38.0	32.0	41.0	11.0	41.0
18	33.47455107657628	38.0	32.0	41.0	10.0	41.0
19	33.39119186165755	39.0	32.0	41.0	10.0	41.0
20	33.33460983265846	39.0	32.0	41.0	10.0	41.0
21	33.269551340275456	38.0	32.0	41.0	2.0	41.0
22	33.18654567757991	38.0	32.0	41.0	2.0	41.0
23	32.92962455097891	38.0	32.0	41.0	2.0	41.0
24	32.84882224136483	38.0	32.0	41.0	2.0	41.0
25	32.65951748911508	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.26443198535533696
1101	2	0.5177850979013101
1101	3	-1.728391708927873
1101	4	-0.4264790302402801
1101	5	0.29695770008267885
1101	6	0.7325675503058307
1101	7	1.034155221590872
1101	8	1.042120661177016
1101	9	1.760610267673549
1101	10	1.5491893905908753
1101	11	1.4842864890034306
1101	12	1.6177855318475451
1101	13	1.347878281899412
1101	14	1.6087269649882643
1101	15	1.6607363914369202
1101	16	1.436097856406505
1101	17	1.5160889102984711
1101	18	1.4860867530442974
1101	19	1.5817704319695238
1101	20	1.8165755104695478
1101	21	1.6279357185896117
1101	22	1.6967990882993647
1101	23	1.9480320832842466
1101	24	1.9284965503600802
1101	25	2.077940663004213
1102	1	0.6203827637199062
1102	2	0.6379946491453445
1102	3	0.6213137959500443
1102	4	0.7843768099259023
1102	5	0.7402654278731617
1102	6	1.0502308248656576
1102	7	1.0085559546122909
1102	8	1.1020159796930287
1102	9	1.68955174833917
1102	10	1.2520262399794504
1102	11	1.1477367731433716
1102	12	1.3010008788559944
1102	13	1.134699585962096
1102	14	1.1051371682950162
1102	15	1.162128065242392
1102	16	1.2131766593473756
1102	17	1.1022102981439943
1102	18	1.278212734825452
1102	19	1.0847608832358304
1102	20	1.3003225804235186
1102	21	1.6558570568557798
1102	22	1.4124559677493522
1102	23	1.7433579734377247
1102	24	1.8720678646210445
1102	25	1.5847311525533243
1103	1	-1.7364968431596992
1103	2	-1.6857154245647301
1103	3	-1.5985879239516763
1103	4	-1.6262497257006316
1103	5	-3.6568475942398564
1103	6	-6.954068929434097
1103	7	-8.745436674380336
1103	8	-11.27114126846422
1103	9	-11.552462993675572
1103	10	-8.115909877956671
1103	11	-11.58782833742174
1103	12	-11.998753420898304
1103	13	-12.196995745733236
1103	14	-12.06654587838803
1103	15	-13.117970214855891
1103	16	-6.991061669890954
1103	17	-5.117691421757726
1103	18	-4.007106675493958
1103	19	-3.2297354312604796
1103	20	-9.158215503114565
1103	21	-5.19242304142432
1103	22	-10.94288801259463
1103	23	-10.838337358257608
1103	24	-12.979897737344555
1103	25	-14.047639854817682
1104	1	0.26947968935483146
1104	2	0.4873024502022396
1104	3	0.5446295341029384
1104	4	0.6086307436490799
1104	5	0.5440379195226157
1104	6	0.5004216945378275
1104	7	0.43697093610327187
1104	8	0.8644958628001689
1104	9	1.2741627328295237
1104	10	1.008391032230847
1104	11	0.8974633612294269
1104	12	0.9303780628170131
1104	13	0.7822234668997297
1104	14	0.8615027015001147
1104	15	0.9465105396128024
1104	16	0.5611781590376594
1104	17	0.4580021849973974
1104	18	0.7286784983189634
1104	19	0.9069571885443253
1104	20	1.2068601630769038
1104	21	0.9914512869011531
1104	22	0.6260203106864353
1104	23	1.2261433962110857
1104	24	1.414608239187963
1104	25	1.2799042837940107
1105	1	0.5012043583819015
1105	2	0.6440283976977952
1105	3	0.19856691622062073
1105	4	0.576963831315922
1105	5	0.7228565544792573
1105	6	0.7799483002190399
1105	7	0.6709938402421969
1105	8	0.6313468294727542
1105	9	0.8779382758778169
1105	10	0.6395310651401331
1105	11	1.2871149860876727
1105	12	1.2649622898248865
1105	13	1.28826836671292
1105	14	1.057603974253098
1105	15	0.7487833557630168
1105	16	0.9923604566109745
1105	17	0.8604881293644695
1105	18	1.1007203478567575
1105	19	1.2173287265268655
1105	20	1.3348416267879486
1105	21	1.3374454691175117
1105	22	1.143458620821832
1105	23	1.527792770964794
1105	24	1.5579621583707066
1105	25	1.56149072781664
1106	1	-0.14314762059699504
1106	2	0.06104998231303682
1106	3	-0.1077809780885488
1106	4	-1.4538657738222973
1106	5	-0.24340133388348306
1106	6	-0.2463740026380492
1106	7	0.025573994102241926
1106	8	0.1610883309002098
1106	9	0.9111620205112345
1106	10	0.3537870917403012
1106	11	0.26427414810770955
1106	12	0.4797109985773531
1106	13	0.2897253982912744
1106	14	-0.5181546628619813
1106	15	0.19697701020369607
1106	16	0.06391222637508065
1106	17	0.03305764191268423
1106	18	-0.19833071362810983
1106	19	-1.5198841090496096
1106	20	-0.16415977731726628
1106	21	-0.14291106775504403
1106	22	0.34066379764257704
1106	23	0.22317665831258893
1106	24	0.2613348128767612
1106	25	0.6105005378732713
1107	1	0.15608528525423893
1107	2	-0.09958446229116902
1107	3	-0.10963994323038406
1107	4	-0.06185578630669397
1107	5	0.06836846027236021
1107	6	-0.09749588638938889
1107	7	-0.04149367953240457
1107	8	-0.2869644915543539
1107	9	0.7529217495519447
1107	10	0.3930923933856363
1107	11	0.5375317534319564
1107	12	0.3393026424776835
1107	13	0.27993074033401655
1107	14	0.43740819617847393
1107	15	0.1953919257457386
1107	16	0.1230248073125253
1107	17	-0.09773407867424311
1107	18	0.10497160271777517
1107	19	0.16539503242574227
1107	20	0.4042533351769393
1107	21	0.4345366837986617
1107	22	0.2887542786911723
1107	23	0.4277974819174517
1107	24	0.5979649849423012
1107	25	0.36587601687917726
1108	1	-0.1775287841916402
1108	2	-0.0886638275131304
1108	3	0.010134434770684209
1108	4	-0.21808019253109734
1108	5	0.026506214113950932
1108	6	0.06804409267892453
1108	7	-0.2764563303999914
1108	8	0.28383416151120855
1108	9	0.5617876205750392
1108	10	0.25755449550770493
1108	11	0.645279770686372
1108	12	0.5946126724677896
1108	13	0.03089123215374201
1108	14	0.12172193487978689
1108	15	-0.15912500601068302
1108	16	-0.8230641268934065
1108	17	-0.8332933873596886
1108	18	-0.664661958049237
1108	19	-0.4748214263464732
1108	20	-0.020930490829556447
1108	21	-0.168467267468543
1108	22	-0.10228604699266697
1108	23	-0.15651917643942426
1108	24	0.02194501449820052
1108	25	-0.0792737614515886
1109	1	0.1101746839559965
1109	2	0.07796860886020696
1109	3	0.09587677236373082
1109	4	0.08517279687241341
1109	5	0.04027399176204938
1109	6	0.3958834007773291
1109	7	0.48418449100579153
1109	8	0.3250605710287999
1109	9	1.1864467702187653
1109	10	0.26505585587766234
1109	11	0.31612404887179224
1109	12	0.5223755118592521
1109	13	0.6032958812086022
1109	14	0.3053555853548886
1109	15	-0.09764045208319061
1109	16	0.3141862130096129
1109	17	0.04131536417688153
1109	18	0.14894878970709868
1109	19	0.5311024353762761
1109	20	0.8036276352399838
1109	21	0.5496693818834046
1109	22	0.18396269684399158
1109	23	0.6986487894626521
1109	24	0.5034739187599797
1109	25	0.3252991278529507
1110	1	0.004099713480282929
1110	2	0.13868966371249414
1110	3	-2.552748693318563E-6
1110	4	0.16834123822085445
1110	5	0.0494661463018673
1110	6	0.2808800781325189
1110	7	0.17581291439036306
1110	8	-0.004020421880166936
1110	9	-3.3078638051680613
1110	10	-1.4837681957546742
1110	11	-0.5168912829201489
1110	12	0.04859277934295392
1110	13	-0.1918416718216278
1110	14	0.1009478160960171
1110	15	0.1564019750772161
1110	16	-0.44425006774159215
1110	17	-0.1668470309253962
1110	18	0.07065678541184184
1110	19	-0.24443002496592925
1110	20	0.07718585668365563
1110	21	-0.13701674687447962
1110	22	0.23699739150669785
1110	23	0.48549200899757494
1110	24	0.40556119455587236
1110	25	0.6122323899460014
1111	1	0.01733803164291814
1111	2	0.15502448069130637
1111	3	0.06337914124119592
1111	4	0.10708548747329161
1111	5	0.25063605114701204
1111	6	0.27974564309015193
1111	7	0.4109754618705068
1111	8	0.526766468959643
1111	9	0.9660910329429697
1111	10	0.4174760909131692
1111	11	0.7526013941370238
1111	12	0.6737031547840786
1111	13	0.44562775979349567
1111	14	0.20987528109764497
1111	15	0.5639043621799757
1111	16	0.5323705430573895
1111	17	0.4952118040487221
1111	18	0.07920620517376165
1111	19	0.5244378148482483
1111	20	0.6881921215188669
1111	21	0.5397560697870531
1111	22	0.6312435010530137
1111	23	0.9899392038899251
1111	24	0.888475824577398
1111	25	1.1774616565741525
1112	1	-0.5193533410999969
1112	2	-0.5026134551499091
1112	3	-0.3838709215251761
1112	4	-0.3355988030664676
1112	5	-0.5665239669050557
1112	6	-0.6318523502342792
1112	7	-0.3356051185990587
1112	8	-0.64317868170275
1112	9	0.010908147907855437
1112	10	-0.6403159498487767
1112	11	-0.38447497135887687
1112	12	-0.8564505034557222
1112	13	-0.5960702900591777
1112	14	-1.4180144749683556
1112	15	-0.5864257196242448
1112	16	-0.799191854417785
1112	17	-0.7370272380209144
1112	18	-0.7299566839584912
1112	19	-0.447132869328307
1112	20	-0.5379697494889228
1112	21	-0.8262049950386725
1112	22	-0.6701536860893498
1112	23	-0.10722737575098051
1112	24	-0.38201951376800736
1112	25	-0.45979915734011456
1113	1	-2.1605699767300415
1113	2	-2.2561826668196794
1113	3	-2.0145351052619844
1113	4	-1.9017068105152575
1113	5	-1.5503849228330502
1113	6	-1.6219206308053522
1113	7	-2.6633518597536217
1113	8	-2.0770706742539637
1113	9	-11.31249346599655
1113	10	-4.6247976382410805
1113	11	-3.193910104816357
1113	12	-12.405178001903892
1113	13	-4.753115603529075
1113	14	-4.075370154670406
1113	15	-3.533663460158074
1113	16	-2.477031705441995
1113	17	-2.6693673994113567
1113	18	-2.7336810787443397
1113	19	-3.4483743342569255
1113	20	-13.590732008955094
1113	21	-16.81316961328821
1113	22	-11.594424325443793
1113	23	-18.161851832610072
1113	24	-19.382640246232313
1113	25	-16.379724669444396
1114	1	-0.3988345054973621
1114	2	-0.4168533342740659
1114	3	-0.26525459061834056
1114	4	-0.8942614758552381
1114	5	-0.4097078292429508
1114	6	-0.48158902838276774
1114	7	-0.45871744123252967
1114	8	-0.21416847934857586
1114	9	0.360647705149411
1114	10	-0.5939556417167218
1114	11	-0.5849681024186459
1114	12	-0.6482586775464156
1114	13	-0.5810402726349082
1114	14	-0.5800740169161642
1114	15	-0.6297203694631861
1114	16	-0.6582694620987439
1114	17	-0.6360896901559911
1114	18	-0.582632340305338
1114	19	-1.0154497169747643
1114	20	-1.407210555681047
1114	21	-1.480765651195501
1114	22	-1.4777519581728598
1114	23	-1.2582507781710284
1114	24	-0.7096441813767136
1114	25	-0.9300610050959754
1115	1	-0.3191414712588738
1115	2	-0.11458201252222722
1115	3	-0.2626965426057488
1115	4	-0.01600769500405619
1115	5	-0.31697156118200454
1115	6	-0.5449780203431907
1115	7	-0.5932820108620369
1115	8	-0.3297304065988129
1115	9	0.015423665120174235
1115	10	-0.29123455328134895
1115	11	-0.16825927012539665
1115	12	-0.3349399616302975
1115	13	-0.3723204210579141
1115	14	-0.45394257260290516
1115	15	-0.6008509704639167
1115	16	-0.505376900115273
1115	17	-0.4922486826786212
1115	18	-0.5919649989886437
1115	19	-0.5278464111896426
1115	20	-1.0116688679315686
1115	21	-0.3444301252495876
1115	22	-0.853897457694984
1115	23	-0.5148426711265586
1115	24	0.004163655807751354
1115	25	-0.08910974856030407
1116	1	-0.6746507608532575
1116	2	-0.643457810056475
1116	3	-0.6520638597805508
1116	4	-0.5251976447892162
1116	5	-1.4477973733609915
1116	6	-1.2612068043469087
1116	7	-2.479422956640274
1116	8	-1.4550229238931927
1116	9	-0.4708540655193296
1116	10	-1.647065423178713
1116	11	-1.072744635956962
1116	12	-2.020492551333085
1116	13	-1.6211412932803206
1116	14	-1.256530765623559
1116	15	-2.845126690094208
1116	16	-3.006668505510575
1116	17	-3.673318020223199
1116	18	-2.808867893815279
1116	19	-2.034780769553901
1116	20	-2.485494042989014
1116	21	-1.810011974230644
1116	22	-1.564051695459078
1116	23	-1.7982304063859509
1116	24	-2.2143780907507384
1116	25	-1.8181292758825514
1201	1	0.663344264546577
1201	2	0.7096142769447482
1201	3	0.2848532291524002
1201	4	0.5473826656283904
1201	5	0.8340019120590867
1201	6	0.746759580383177
1201	7	0.8876214348013676
1201	8	0.9644591174170642
1201	9	1.5965552789382045
1201	10	1.1708907122147068
1201	11	1.0607785176457867
1201	12	1.3417584392764752
1201	13	1.0893508664560727
1201	14	1.2908341011829592
1201	15	1.4778758866081887
1201	16	1.1644116021553543
1201	17	1.1304359190662439
1201	18	1.052605615959905
1201	19	1.1960959159191802
1201	20	1.4569388493993358
1201	21	1.5605845627068788
1201	22	1.4705015005139828
1201	23	1.5028270952663974
1201	24	1.7476019789192065
1201	25	1.7428351196460596
1202	1	0.9955605731082429
1202	2	1.0235322243380942
1202	3	1.0623591144908282
1202	4	1.1432258833847797
1202	5	1.3233743163359577
1202	6	1.347476470114934
1202	7	1.3767310097201957
1202	8	1.4421004958211014
1202	9	2.1504738154318375
1202	10	1.839268035478959
1202	11	1.8808718220678955
1202	12	1.828076585151301
1202	13	1.756697264221117
1202	14	1.6265396960496332
1202	15	1.6845651203473224
1202	16	1.586833891897534
1202	17	1.8137967815090619
1202	18	1.7597978507118412
1202	19	1.6249915114882398
1202	20	1.9290981964949552
1202	21	1.927592907539001
1202	22	1.8009867605451149
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2211	5	-0.9094842766690583
2211	6	-0.08211738594544471
2211	7	0.14819942359532945
2211	8	0.18508448999707383
2211	9	0.6324634183110334
2211	10	0.10315393374302317
2211	11	0.4756712109550776
2211	12	0.48532022024722465
2211	13	0.32077695497341807
2211	14	0.1663389311332537
2211	15	0.303234822070948
2211	16	0.19546534780204894
2211	17	0.04025714633110766
2211	18	0.14792626643261286
2211	19	-0.23778495041461412
2211	20	0.42830354475749033
2211	21	0.4332477518385289
2211	22	0.38491736437789825
2211	23	0.835643832964962
2211	24	0.9282367932329905
2211	25	1.0039315282119148
2212	1	0.10775056630509994
2212	2	0.18494476131284898
2212	3	0.11965798994641474
2212	4	0.28969141306659196
2212	5	0.03796332339715036
2212	6	0.14378386407086907
2212	7	-0.009871563815224249
2212	8	0.0328460192187201
2212	9	0.6728868621090669
2212	10	0.0927216785009719
2212	11	0.1830962432854477
2212	12	0.15371426417807754
2212	13	-0.07622873366187832
2212	14	0.008779941503007649
2212	15	0.02357449190620997
2212	16	0.04484528449426506
2212	17	-0.01281480601154783
2212	18	0.07834092625799371
2212	19	0.006831174871344103
2212	20	0.3681038003786483
2212	21	0.5031490128433873
2212	22	-0.05819353550884898
2212	23	0.44480546836347656
2212	24	0.5248560282787764
2212	25	0.4180288771554004
2213	1	-0.0012429208943522951
2213	2	0.011422825038945206
2213	3	-0.25140594177269904
2213	4	0.012307372643000747
2213	5	-0.15003487899358703
2213	6	0.1918338749172932
2213	7	0.1414667859596861
2213	8	0.15025626064527486
2213	9	0.7615095641334264
2213	10	0.024963680234989738
2213	11	0.5718023956455554
2213	12	0.24433136563206403
2213	13	-5.818169080029634E-4
2213	14	-0.03182631352715504
2213	15	0.2423588727732593
2213	16	-0.2346179482487969
2213	17	-0.1091225702844838
2213	18	-0.28782197642873797
2213	19	-0.34253814268014793
2213	20	0.25865843773712527
2213	21	-0.21085905520008197
2213	22	-0.10351639503600296
2213	23	-0.48071422372527195
2213	24	-0.27798990374700594
2213	25	0.1287459211621993
2214	1	-1.0029079854615794
2214	2	-0.9603321380175451
2214	3	-0.7968172480717364
2214	4	-0.6768597451348768
2214	5	-0.9981615653044216
2214	6	-0.791469058995748
2214	7	-0.7918899242183848
2214	8	-0.597177761662234
2214	9	-10.712903503284728
2214	10	-3.9225391157022997
2214	11	-1.9642859059814448
2214	12	-1.215499264128706
2214	13	-1.1252509821832497
2214	14	-1.0726730647408687
2214	15	-0.9954264877991008
2214	16	-1.2754764831062388
2214	17	-1.1925132744893148
2214	18	-1.5203057685624088
2214	19	-1.6778028033183503
2214	20	-1.38189829300876
2214	21	-1.7164459306491082
2214	22	-1.3927098653262213
2214	23	-1.6665062202744494
2214	24	-2.273462842073556
2214	25	-9.72980893277061
2215	1	-0.5354467946520245
2215	2	-0.3657787698823114
2215	3	-0.2447672311479323
2215	4	-0.4278319319438566
2215	5	-0.4508397759439049
2215	6	-0.40393481997795533
2215	7	-0.3417458109437099
2215	8	-0.31557572603427175
2215	9	0.5692342042928118
2215	10	0.47086179356076485
2215	11	0.5267567792820245
2215	12	0.45683352197677607
2215	13	0.31798456585724466
2215	14	0.1451687252757239
2215	15	-0.37297791901613664
2215	16	0.1357645014267419
2215	17	0.10751259581917338
2215	18	0.0693713819163051
2215	19	0.015456602067146719
2215	20	0.2659769867558879
2215	21	0.28382246448220627
2215	22	0.35913646951459555
2215	23	0.5604528032444165
2215	24	0.6508161815049576
2215	25	0.9232769402516539
2216	1	-0.6822260384357222
2216	2	-0.6633726615875233
2216	3	-0.6264374158495052
2216	4	-0.42096958288272646
2216	5	-1.3263271328281583
2216	6	-1.886944513064968
2216	7	-1.1776187672119747
2216	8	-2.4160671130124207
2216	9	-0.7744624443759314
2216	10	-1.1381565134963587
2216	11	-0.4935067224807881
2216	12	-1.729720188269905
2216	13	-1.251925710028761
2216	14	-1.4283077569928082
2216	15	-1.0591676880775367
2216	16	-2.05172494920982
2216	17	-2.5024346241746436
2216	18	-2.2155727562410057
2216	19	-3.7549482848721247
2216	20	-2.470169636594381
2216	21	-2.6631316379637298
2216	22	-2.495833631929976
2216	23	-2.104308245376533
2216	24	-2.309697242974874
2216	25	-2.2872100278470704
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40605.0
3	2.0
4	544.0
5	5655.0
6	7865.0
7	6052.0
8	4840.0
9	3831.0
10	3307.0
11	3023.0
12	2659.0
13	2468.0
14	2477.0
15	2532.0
16	2602.0
17	2691.0
18	3141.0
19	3927.0
20	5183.0
21	6843.0
22	8235.0
23	9565.0
24	11044.0
25	12417.0
26	13870.0
27	15209.0
28	16881.0
29	19996.0
30	23976.0
31	29908.0
32	36673.0
33	45753.0
34	56684.0
35	71523.0
36	93733.0
37	142456.0
38	305724.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.990477102256854	18.40865618509668	12.532946217315105	23.067920495331357
2	16.459578191933023	21.709031740109662	38.74485046069942	23.086539607257897
3	18.967448683502443	28.63087965418194	29.109739220288095	23.291932442027523
4	12.171659852597323	17.522966962344537	36.12000445372521	34.18536873133293
5	12.275288802738741	39.084800143815166	33.94067233590221	14.699238717543887
6	29.2811694768572	37.30803868822611	18.81421094890083	14.59658088601586
7	26.399615502591644	33.11337398429578	22.63503810783511	17.85197240527746
8	26.632864400156308	34.131105900742476	20.634036733098867	18.601992966002346
9	26.91820691838278	15.556981985942823	20.318621560202203	37.2061895354722
10	15.730085685533115	30.559447391818352	31.65518656388311	22.055280358765426
11	33.544603094888686	23.428569195325032	23.529595679114877	19.497232030671405
12	23.252588396171127	27.422836491502245	30.163996874389532	19.1605782379371
13	28.59659934971769	22.090748469277628	27.120034614005977	22.19261756699871
14	21.929624073075633	21.61042609218844	29.549157270447036	26.910792564288894
15	23.513795447619497	28.513619634778127	26.174136758948872	21.798448158653507
16	21.9608655180733	28.458795295579325	27.21480597341481	22.36553321293257
17	21.38304023879944	27.699973041293706	28.82989447033956	22.087092249567295
18	20.58858580767172	25.725695217776945	32.08737257108472	21.598346403466614
19	22.088534269029612	25.348102069563755	32.10490634896756	20.45845731243907
20	22.230948592237894	24.739552044168455	32.129582687402866	20.899916676190784
21	22.989207463055415	24.327114771680556	31.863038613429083	20.820639151834946
22	22.264712317634462	24.51766415828586	31.985762399293588	21.231861124786082
23	21.734497231395554	24.593158240712796	32.54724306774461	21.125101460147043
24	21.96798104374648	24.82382346305201	32.086740878133206	21.12145461506831
25	21.33895304102641	24.605970303931546	32.63292300081441	21.42215365422764
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1668.0
1	1668.0
2	2211.5
3	2755.0
4	2755.0
5	2755.0
6	9582.5
7	16410.0
8	16410.0
9	16410.0
10	13465.5
11	10521.0
12	10521.0
13	10521.0
14	8706.0
15	6891.0
16	6891.0
17	6891.0
18	10955.0
19	15019.0
20	15019.0
21	15019.0
22	22246.0
23	29473.0
24	29473.0
25	29473.0
26	42235.0
27	54997.0
28	54997.0
29	54997.0
30	63241.5
31	71486.0
32	71486.0
33	71486.0
34	79777.5
35	88069.0
36	88069.0
37	88069.0
38	92018.0
39	95967.0
40	95967.0
41	95967.0
42	102601.5
43	109236.0
44	109236.0
45	109236.0
46	116613.0
47	123990.0
48	123990.0
49	123990.0
50	121038.0
51	118086.0
52	118086.0
53	118086.0
54	108795.0
55	99504.0
56	99504.0
57	99504.0
58	86756.0
59	74008.0
60	74008.0
61	74008.0
62	61993.0
63	49978.0
64	49978.0
65	49978.0
66	38830.5
67	27683.0
68	27683.0
69	27683.0
70	21398.0
71	15113.0
72	15113.0
73	15113.0
74	10961.0
75	6809.0
76	6809.0
77	6809.0
78	5332.5
79	3856.0
80	3856.0
81	3856.0
82	2629.0
83	1402.0
84	1402.0
85	1402.0
86	1024.0
87	646.0
88	646.0
89	646.0
90	470.5
91	295.0
92	295.0
93	295.0
94	162.5
95	30.0
96	30.0
97	30.0
98	16.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02500258815853985
2	0.0015626617599087407
3	2.9299907998288886E-4
4	0.0031253235198174813
5	0.034964556877958075
6	0.011719963199315554
7	0.021486599198745182
8	0.028713909838323108
9	0.035159889597946664
10	0.037503882237809774
11	0.039066543997718514
12	0.009180637839463851
13	0.0030276571598231846
14	0.00761797607955511
15	0.00761797607955511
16	0.008887638759480962
17	0.010352634159395406
18	0.01816594295893911
19	0.019533271998859257
20	0.01728694571899044
21	0.012794293159252814
22	0.012305961359281333
23	0.009082971479469555
24	0.006152980679640666
25	0.10401467339392553
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1023894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.2176522718681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.31435519390297	39.32763057022986
2	11.880977432661739	11.932695867266387
3	4.007331554634127	6.037163476461035
4	1.7291288098923403	3.473311572337706
5	0.9632527069875734	2.41861447447188
6	0.5987343134977954	1.8040218935080767
7	0.4096972666686641	1.4401824412011253
8	0.29819028100538714	1.1979532673903333
9	0.22295629903294054	1.0076707706992962
>10	1.3653703130730575	13.06209686768346
>50	0.13715136088348678	4.772672324331021
>100	0.0674001226987605	5.73516700447637
>500	0.003116768606402892	1.0723357403068854
>1k	0.0013635862653012653	1.3516204090330446
>5k	3.895960758003615E-4	1.6207314856234698
>10k+	5.843941137005423E-4	3.746131834980129
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACTTTTTTTTTTTTTTTTTTTTT	14314	1.3979962769583572	No Hit
GTATCAACGCAGAGTACTTTTTTTT	13631	1.3312901530822527	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10350	1.0108468259409666	No Hit
GGTATCAACGCAGAGTACTTTTTTT	9966	0.9733429437031568	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	6602	0.6447933086823441	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4149	0.40521772761633534	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2230	0.21779598278728074	No Hit
GTGGTATCAACGCAGAGTACATGGG	1741	0.17003713275006982	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1658	0.16193082487054325	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1467	0.14327655011163265	No Hit
GTATAACGCAGAGTACTTTTTTTTT	1391	0.13585390675206613	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1181	0.1153439711532639	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	9.766635999429628E-5	0.0	0.0	0.0	0.0
3	9.766635999429628E-5	0.0	0.0	0.0	0.0
4	9.766635999429628E-5	0.0	0.0	0.0	0.0
5	9.766635999429628E-5	0.0	0.0	0.0	0.0
6	9.766635999429628E-5	0.0	0.0	0.0	0.0
7	9.766635999429628E-5	0.0	0.0	0.0	0.0
8	9.766635999429628E-5	0.0	0.0	0.0	0.0
9	9.766635999429628E-5	0.0	0.0	0.0	0.0
10	9.766635999429628E-5	0.0	0.0	0.0	0.0
11	9.766635999429628E-5	0.0	0.0	0.0	0.0
12	9.766635999429628E-5	0.0	0.0	0.0	0.0
13	9.766635999429628E-5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTCG	40	2.7670618E-4	16.6194	17
TACGGTG	35	0.0021630782	16.292963	5
GAACGGC	35	0.002166613	16.288982	6
GGCGTAG	55	1.1259364E-5	15.547814	10
TATCACG	75	5.8313162E-8	15.201564	2
TGAACCG	40	0.005261911	14.256344	5
CGTAAGA	40	0.00527387	14.251467	2
TATAACG	210	0.0	14.025253	2
CCAATAC	75	9.63626E-7	13.93681	3
TAACGCA	230	0.0	13.634502	4
TGCTAGC	70	7.24904E-6	13.5728245	2
ATAACGC	240	0.0	13.461692	3
GTGCACG	150	0.0	13.298769	11
ATCGTCT	50	0.0015027468	13.295522	15
GTCTCGG	50	0.0015033002	13.294871	18
GCCCCCG	155	0.0	12.866005	18
ACGCCGA	75	1.4743704E-5	12.669208	7
TTACCGC	60	4.1015423E-4	12.662401	16
GTGCTAG	70	1.0901462E-4	12.214348	1
CGCGGGC	70	1.0936326E-4	12.210172	13
>>END_MODULE