FastQCFastQC Report
Thu 26 May 2016
SRR1364325_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364325_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817381
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT125930.6929201967006368No Hit
TATCAACGCAGAGTACTTTTTTTTT71700.39452376799361283No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA57060.3139682873321554No Hit
GGTATCAACGCAGAGTACTTTTTTT54300.29878159835499546No Hit
GTACTTTTTTTTTTTTTTTTTTTTT53750.29575526540664837No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN46680.256853130961532No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35570.1957212054049206No Hit
GAGTACTTTTTTTTTTTTTTTTTTT34860.1918144846897816No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA32500.17882876512960133No Hit
GAACAAAAAAAAAAAAAAAAAAAAA24540.13502947373170512No Hit
GTGGTATCAACGCAGAGTACATGGG19310.10625179860469544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC1300.017.5298463
CGCGCCA350.002171903216.2840110
TAACGCA1450.015.0769674
TCACGCA1301.8189894E-1213.8919884
GGTATCA26500.013.6517821
TATAACG1550.013.4772482
TAGCGCT500.001493726913.3070614
ATCACGC1153.8016879E-1013.2108993
TATCACG1301.4551915E-1113.1473852
AGAACCG751.4708528E-512.6733915
CGCATAT1551.8189894E-1212.257468
GTCGAAA550.003052810212.09933616
AACCGAT550.003069916712.0903127
GACCGCG550.003069916712.0903127
ACCGAGT658.027871E-411.691738
GTCGTGC1157.0045644E-811.57359817
GGACCGA855.324933E-511.1762286
CGAGGTT951.3524463E-511.005844
CGCAGAT6050.010.9911928
TGGTATC7450.010.9610572
Produced by FastQC (version 0.11.5)