##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364325_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1817381
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.47536812589105	30.0	26.0	31.0	7.0	34.0
2	26.163666286816028	30.0	26.0	31.0	7.0	34.0
3	25.71336225040319	30.0	25.0	31.0	7.0	34.0
4	29.434294735116083	35.0	28.0	37.0	7.0	37.0
5	30.076718640725307	35.0	30.0	37.0	7.0	37.0
6	30.162206493850217	35.0	30.0	37.0	2.0	37.0
7	29.710018427616443	35.0	30.0	37.0	2.0	37.0
8	29.212763311600593	35.0	28.0	37.0	2.0	37.0
9	30.216166010319245	37.0	28.0	39.0	2.0	39.0
10	29.59376212252687	35.0	27.0	39.0	2.0	39.0
11	30.25083678106022	35.0	28.0	39.0	2.0	39.0
12	29.798047299933256	35.0	27.0	39.0	2.0	39.0
13	29.387867486234313	35.0	27.0	39.0	2.0	39.0
14	29.706871041350162	36.0	25.0	40.0	2.0	41.0
15	30.44489240285884	36.0	27.0	40.0	2.0	41.0
16	30.811360963936565	37.0	29.0	40.0	2.0	41.0
17	31.000095742169638	37.0	30.0	40.0	2.0	41.0
18	30.84574946034981	37.0	29.0	40.0	2.0	41.0
19	30.839858565705264	37.0	29.0	40.0	2.0	41.0
20	30.734138851457125	37.0	28.0	40.0	2.0	41.0
21	30.638881445332597	37.0	28.0	40.0	2.0	41.0
22	30.7313359169046	37.0	29.0	40.0	2.0	41.0
23	30.73662869811008	37.0	30.0	40.0	2.0	41.0
24	30.612577659830272	37.0	29.0	40.0	2.0	41.0
25	30.441237693141943	37.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6663316132979169
1101	2	0.8786723324174055
1101	3	1.516482440695178
1101	4	0.8551951151569313
1101	5	0.31474392417995745
1101	6	0.22870245575331438
1101	7	0.5612553811132273
1101	8	0.7076952898860007
1101	9	0.7042922676249539
1101	10	1.088544129055773
1101	11	0.508385802241051
1101	12	0.8530354634118638
1101	13	0.8998988929245577
1101	14	1.3055726565200985
1101	15	0.6233865341544735
1101	16	0.14337631063444078
1101	17	-0.5120231689618961
1101	18	-6.0496227336271495
1101	19	-7.21142218933133
1101	20	-8.48548529624873
1101	21	-10.013728678300485
1101	22	-10.451512905654468
1101	23	-11.818224562506195
1101	24	-11.574541583975893
1101	25	-11.689804490704603
1102	1	2.1034993029816142
1102	2	2.2262796470742963
1102	3	2.972007626319151
1102	4	2.4108839664841284
1102	5	1.8326279130271992
1102	6	1.7502294746374254
1102	7	2.0702516328877607
1102	8	2.303875572381685
1102	9	2.4807124059109817
1102	10	-0.9241984332188977
1102	11	1.129019681296949
1102	12	2.054882511301315
1102	13	2.6776021856436003
1102	14	3.3346717036333544
1102	15	2.7926081901143576
1102	16	2.4572923636611037
1102	17	2.187768099817532
1102	18	2.339336342287183
1102	19	2.072919266859369
1102	20	2.5078513904016653
1102	21	2.520820232467237
1102	22	2.376205962115904
1102	23	2.374377288116296
1102	24	2.5507741299742435
1102	25	2.5618347196147653
1103	1	-0.612912705656818
1103	2	-0.1221916443308686
1103	3	-2.0189654901070533
1103	4	-0.3327848581009327
1103	5	-3.840195242434177
1103	6	-7.4798892024251025
1103	7	-8.144865117763668
1103	8	-2.2813782576277433
1103	9	-7.455281318044445
1103	10	-4.653103598028437
1103	11	-8.41187219634553
1103	12	-2.363546652534879
1103	13	-0.31299705494486574
1103	14	-0.07522675422386982
1103	15	-0.2404044126698146
1103	16	-0.4808780780953761
1103	17	-0.5198819067728238
1103	18	-0.041289538538944015
1103	19	-5.477604666263751
1103	20	-1.564793318558177
1103	21	-6.680854097597955
1103	22	-2.3858486820556912
1103	23	-1.5604403111699163
1103	24	-6.369428726183678
1103	25	-3.3804616638018814
1104	1	1.823891744326339
1104	2	1.9228211443756216
1104	3	2.582947756738676
1104	4	1.9514619146238381
1104	5	1.594209644042838
1104	6	1.5425374626363038
1104	7	1.561716999564549
1104	8	1.9011698008839488
1104	9	1.9836165861958754
1104	10	2.223817429627701
1104	11	1.6533969556550048
1104	12	1.98045802491691
1104	13	2.2349852496351197
1104	14	2.732705061541907
1104	15	1.8884771870904231
1104	16	1.271388603257705
1104	17	1.0289713362561486
1104	18	0.8443581642968567
1104	19	1.1622027282598424
1104	20	-9.260482941710794
1104	21	-3.3278275224481355
1104	22	-0.8231680103572216
1104	23	0.1266654450928364
1104	24	0.6834948733024682
1104	25	1.093835405618318
1105	1	1.5396893830814307
1105	2	1.5382565289000745
1105	3	2.279073995594093
1105	4	1.6964942426945235
1105	5	1.5268844603772678
1105	6	1.4079252353057363
1105	7	1.6160270974706883
1105	8	1.8380109702614185
1105	9	1.8416779425957408
1105	10	2.223420116906471
1105	11	1.8249867975836445
1105	12	2.0661646681977786
1105	13	2.0884069128523457
1105	14	2.822644234555561
1105	15	2.023084110182502
1105	16	1.6821839409836201
1105	17	1.1624507297947773
1105	18	1.5994917831160933
1105	19	1.552900811510117
1105	20	1.6031488754354335
1105	21	1.4807970683046072
1105	22	1.4253373310109367
1105	23	1.4410549853365957
1105	24	1.412610697159316
1105	25	1.452055384007334
1106	1	1.209380041469835
1106	2	1.581268648905997
1106	3	2.170918723132864
1106	4	1.7067174158318963
1106	5	0.9204141289214398
1106	6	0.916801755275678
1106	7	1.4431566608207227
1106	8	1.3435723968346096
1106	9	1.7487517387815856
1106	10	-1.5357300275953136
1106	11	0.40900883229006624
1106	12	1.154377649633613
1106	13	1.9833966342535199
1106	14	0.988599073957392
1106	15	1.6685794795252846
1106	16	1.1590626361889562
1106	17	-5.320142800103117
1106	18	-1.2727112262823184
1106	19	0.28689355342032385
1106	20	0.893572615673186
1106	21	1.083194439674319
1106	22	1.081362944560599
1106	23	0.8193061480656638
1106	24	0.9688610204758454
1106	25	1.2068797763060424
1107	1	0.7104548760848424
1107	2	0.9128847732698411
1107	3	1.4170424859060518
1107	4	0.8548734323019431
1107	5	0.24429177031605676
1107	6	0.4950517355432922
1107	7	0.4452715709370416
1107	8	0.6417531399593628
1107	9	0.6184309121621787
1107	10	-2.2094189972736977
1107	11	-0.26587750729041204
1107	12	-0.0974358161474207
1107	13	0.9498832018638232
1107	14	1.238416463126324
1107	15	1.0387215012483537
1107	16	-5.834024566647287
1107	17	-2.1772044152123122
1107	18	-0.4520599709952009
1107	19	0.2906233192555767
1107	20	0.7038987828986549
1107	21	0.8048664208045118
1107	22	0.405066288450076
1107	23	0.09869388070859841
1107	24	0.25264847891078546
1107	25	0.4437596225829239
1108	1	-0.43469026105831077
1108	2	-3.3855566234102064
1108	3	-0.19568689478201406
1108	4	-0.7572247978535387
1108	5	-0.8622510111479755
1108	6	-0.4539657155878203
1108	7	-0.13834581968955462
1108	8	0.2058686910937979
1108	9	-0.35758574231781637
1108	10	-3.5007158898347157
1108	11	-1.586175473294471
1108	12	-5.884198492305618
1108	13	-1.5976376992987298
1108	14	-5.715846669563675
1108	15	-2.161477624059195
1108	16	-7.285582108265377
1108	17	-2.9250745788168224
1108	18	-1.509733614833447
1108	19	-0.3775977205777288
1108	20	-0.2607677790134275
1108	21	0.16160873976623336
1108	22	0.026937296919459897
1108	23	-0.03902508905587965
1108	24	0.10101609355647412
1108	25	-0.1839604888233275
1109	1	-8.770453210984865
1109	2	-6.509404710467603
1109	3	-5.445590139043979
1109	4	-5.371238938543804
1109	5	-2.6453748802688892
1109	6	-1.6003291845804526
1109	7	-1.100139428564752
1109	8	-0.6800629751043488
1109	9	-5.602388152748233
1109	10	-1.4846034611518597
1109	11	-1.0780333913287485
1109	12	-5.963972237302034
1109	13	-7.718347535026787
1109	14	-8.110160698601284
1109	15	-3.9350454411705584
1109	16	-2.356604284383586
1109	17	-2.0796900007411274
1109	18	-1.3680658923496871
1109	19	-1.0608097834199626
1109	20	-0.8084893917212419
1109	21	-0.8664004379238222
1109	22	-0.9355957138617512
1109	23	-1.217103158811078
1109	24	-0.842033132595585
1109	25	-0.9084865094355159
1110	1	-2.6713005331348043
1110	2	-2.4097030417651553
1110	3	-1.7737524125074735
1110	4	-2.649155865267165
1110	5	-5.6076670582740284
1110	6	-4.067495511099342
1110	7	-9.251497211694698
1110	8	-9.673203700557231
1110	9	-11.289135025367038
1110	10	-8.983025890228177
1110	11	-11.551361864952298
1110	12	-6.537902207703436
1110	13	-4.846017583387759
1110	14	-4.447661320822828
1110	15	-9.80247300501049
1110	16	-11.042730849988711
1110	17	-12.112487537509637
1110	18	-8.702684671570168
1110	19	-11.647181073543678
1110	20	-12.657800736064384
1110	21	-13.385854912366792
1110	22	-14.247081732065084
1110	23	-14.942885339151939
1110	24	-15.281109028307426
1110	25	-15.901510688007356
1111	1	-0.5752134729386285
1111	2	0.08465100000616133
1111	3	-2.516171290541905
1111	4	-3.3292988470678857
1111	5	-1.097620985008433
1111	6	-0.45417537877413494
1111	7	0.04506857615018589
1111	8	0.18423781396126415
1111	9	-4.197675884179823
1111	10	-3.915219283281175
1111	11	-1.198345036006991
1111	12	-5.825910311768315
1111	13	-1.183960739335081
1111	14	0.28952859608429904
1111	15	0.28999036909511133
1111	16	0.2919382234984873
1111	17	0.011734775766573335
1111	18	0.40687850875580267
1111	19	0.3934848020297217
1111	20	0.47143244557903685
1111	21	0.651916203025575
1111	22	0.34305106817341624
1111	23	0.20157654387312007
1111	24	0.3564701794963625
1111	25	0.556089341570889
1112	1	-0.1456925188359044
1112	2	0.32847450415741974
1112	3	-2.079099964942074
1112	4	-0.1558514679189038
1112	5	-0.4237257225163802
1112	6	-0.2810112095362207
1112	7	0.14308736075142292
1112	8	-3.2309835239299254
1112	9	-0.5740932054588725
1112	10	-3.1761329794970194
1112	11	-0.8273198232269117
1112	12	0.22356139646064932
1112	13	0.6761501265798131
1112	14	1.2235307496153034
1112	15	0.5956430685682292
1112	16	0.45434396511214814
1112	17	0.22695336910056696
1112	18	0.36632755782588333
1112	19	0.47291181419828376
1112	20	0.4574021981926961
1112	21	0.4090102327382432
1112	22	0.3607124120553138
1112	23	0.28438350189346195
1112	24	0.3382438353908803
1112	25	0.2833965263721012
1113	1	-1.2796386821938341
1113	2	-0.7481836019124515
1113	3	-3.3688276370226866
1113	4	-1.435731541916624
1113	5	-1.6139806186536312
1113	6	-1.452767645998538
1113	7	-1.4046491281615374
1113	8	-8.932190548666362
1113	9	-9.727249270379644
1113	10	-3.7470063738998256
1113	11	-9.274193652453683
1113	12	-7.507481540464642
1113	13	-3.2738101763216676
1113	14	-1.8862875366735707
1113	15	-7.302191741031287
1113	16	-3.4348739535175348
1113	17	-2.6896623416327934
1113	18	-1.9408474258196868
1113	19	-1.7598044185179482
1113	20	-6.493452017367403
1113	21	-2.7688919745757268
1113	22	-2.416303453195848
1113	23	-2.341917330289725
1113	24	-7.702224881548425
1113	25	-4.644356695498768
1114	1	-4.388841783724303
1114	2	-4.584784909101991
1114	3	-1.1846032226192413
1114	4	-0.882489955426955
1114	5	-0.9636335232937476
1114	6	-1.1388391760598395
1114	7	-0.8812049678887313
1114	8	-0.44536393396250773
1114	9	-0.7202058733106718
1114	10	-3.5307111601621592
1114	11	-1.5213011911213243
1114	12	-4.793987447175152
1114	13	-1.5003964630102544
1114	14	-6.099822225911584
1114	15	-2.406949586198337
1114	16	-1.4985284008036928
1114	17	-1.468028760664172
1114	18	-1.036369692697452
1114	19	-0.7004531417753661
1114	20	-0.4562661285191112
1114	21	-0.33668720861136947
1114	22	-0.3163765259031912
1114	23	-0.8151037062893991
1114	24	-0.7090652988747763
1114	25	-0.4862151780222135
1115	1	0.42608805294466734
1115	2	0.5742759374588537
1115	3	1.2487416128995044
1115	4	0.1632024761350408
1115	5	0.10355361726296053
1115	6	-0.41901803504304524
1115	7	-2.19374810108404
1115	8	-0.19895836690476898
1115	9	-0.10666492553059115
1115	10	0.7726014692374292
1115	11	-0.23334397925106742
1115	12	0.34265851555776905
1115	13	0.6770535024831865
1115	14	1.2151363662209214
1115	15	0.38353810646326636
1115	16	0.16734749061567555
1115	17	-0.3754505458033499
1115	18	-0.051063734565406804
1115	19	0.17703420582067508
1115	20	0.11734785813835558
1115	21	0.3009220621500752
1115	22	-0.10434811550521417
1115	23	-0.17134076633080753
1115	24	-0.2631687035216608
1115	25	-0.023266592791021168
1116	1	0.2663991272668902
1116	2	0.5257795746996194
1116	3	1.3057257745901296
1116	4	0.6398157208279827
1116	5	-0.259202039462032
1116	6	-0.8946721874149759
1116	7	-4.311844979061206
1116	8	-0.8198851257865947
1116	9	-0.739989820847125
1116	10	-0.27500456344568036
1116	11	-2.0927553397283525
1116	12	-0.4512395618758944
1116	13	-6.606757348849801
1116	14	-1.5040722475842827
1116	15	-1.176869851042099
1116	16	-0.6246745922599679
1116	17	-0.45555151133806504
1116	18	-0.28188253889192083
1116	19	0.005952764425195767
1116	20	0.1884571298051867
1116	21	0.18964642055711423
1116	22	-0.10100208393271615
1116	23	-0.24584964772309092
1116	24	-0.08013391112724122
1116	25	-0.009037037953888216
1201	1	2.350713005128675
1201	2	2.561162756530713
1201	3	3.050369629094451
1201	4	2.6370812147775595
1201	5	2.128172935681487
1201	6	2.1165866526285697
1201	7	2.3980600251729065
1201	8	2.4890489087009335
1201	9	2.7872756519729798
1201	10	3.019053617264376
1201	11	2.7301406988005397
1201	12	2.9469807025571164
1201	13	3.086259454011973
1201	14	3.6794803118640758
1201	15	3.210270939101612
1201	16	2.7509026238755325
1201	17	2.523223814977314
1201	18	2.6467358776042857
1201	19	2.6055135530992324
1201	20	2.7842512376401594
1201	21	2.5484420217763315
1201	22	1.6683580897797299
1201	23	1.094598292423214
1201	24	1.7540566527785018
1201	25	1.9577819409149981
1202	1	2.2485728822500626
1202	2	2.4171971359020894
1202	3	3.0063089163744685
1202	4	2.6746124207821325
1202	5	1.965560326790655
1202	6	1.8736642908895433
1202	7	2.2074098962177153
1202	8	2.457759905107313
1202	9	2.7607188444633586
1202	10	-0.8826642746956814
1202	11	1.1624862311121937
1202	12	2.2751274107758768
1202	13	2.8022158969267963
1202	14	3.5585860405992378
1202	15	2.71488245485903
1202	16	2.552788967544558
1202	17	2.2726445118536205
1202	18	2.5599398075766473
1202	19	2.7147537853162227
1202	20	2.6721011573827376
1202	21	2.7978945161660747
1202	22	2.5154722651362533
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2211	15	-1.4442838366341348
2211	16	-0.4585171559292718
2211	17	-0.039722900079876666
2211	18	0.4259877445311453
2211	19	0.6241953694885183
2211	20	0.8586159327954128
2211	21	0.9214643460270828
2211	22	0.7029993374808008
2211	23	0.5589183295167715
2211	24	0.7022921395059605
2211	25	0.8250914510386451
2212	1	0.618707874726951
2212	2	0.6692794478887016
2212	3	1.4540563769932824
2212	4	0.7851885785701853
2212	5	0.4207973692317353
2212	6	0.27191698062708625
2212	7	0.4418112185065617
2212	8	0.7227703929925084
2212	9	0.7217694894956495
2212	10	1.4522340353482974
2212	11	0.6198226153383324
2212	12	0.9256434754347538
2212	13	0.9383155754253814
2212	14	-4.47317550410407
2212	15	-1.1246640195491509
2212	16	-0.46882281728773023
2212	17	-0.023272677795510788
2212	18	0.3573734434227305
2212	19	0.7048376778093157
2212	20	0.9616476141434589
2212	21	1.054494224841811
2212	22	0.8465929599943856
2212	23	0.7936246766240416
2212	24	0.8014161981596111
2212	25	0.890467930618076
2213	1	0.27426485849358784
2213	2	0.47015769649257066
2213	3	0.9553203438012048
2213	4	0.319078172005721
2213	5	-0.030282746325159593
2213	6	-0.3026169136895689
2213	7	-0.12454644850726027
2213	8	-5.432220985448229
2213	9	-2.1165712980874183
2213	10	-0.2253527107628912
2213	11	-0.5193567795567482
2213	12	0.0507112451751901
2213	13	-8.298283221975904
2213	14	-2.608941024681947
2213	15	-1.3470995737732103
2213	16	-0.788789107007883
2213	17	-0.7372757701536479
2213	18	-0.25976589926055027
2213	19	-0.14096376958702805
2213	20	0.07054342367093014
2213	21	0.2139173310254847
2213	22	-0.15977663651798935
2213	23	-0.13487322879757357
2213	24	6.840319333818456E-4
2213	25	-0.07640915539947812
2214	1	-0.9244077598028007
2214	2	-0.5621947692933098
2214	3	-6.968953471020743
2214	4	-2.6062647441599935
2214	5	-1.6426958133407759
2214	6	-1.169533557661218
2214	7	-0.7641281073911976
2214	8	-6.004404423178638
2214	9	-2.0077638603215817
2214	10	-0.39296670063537675
2214	11	-0.7127826421334404
2214	12	-0.29009140560274815
2214	13	-6.293074217478971
2214	14	-1.6363011403490368
2214	15	-0.882690563076661
2214	16	-0.8840895779571554
2214	17	-0.6899285408277009
2214	18	-0.8878178254585727
2214	19	-0.4482570622686062
2214	20	-0.08831106473910921
2214	21	-0.18376512030200942
2214	22	-0.2721601446583044
2214	23	-0.45546380848364976
2214	24	-0.24607323766791467
2214	25	-0.17551220405981027
2215	1	0.012540166919716711
2215	2	0.30172545447259225
2215	3	-2.816801321909484
2215	4	-0.8426649574846863
2215	5	-0.49664479543545426
2215	6	-0.8680587369507222
2215	7	-0.3369996422713619
2215	8	0.13843423539609034
2215	9	0.296850073741286
2215	10	0.8806302057065309
2215	11	-0.06525747859576114
2215	12	-0.14156555123510728
2215	13	0.5456703172009192
2215	14	0.6769404048598204
2215	15	0.34396478477985326
2215	16	-0.13774388844359464
2215	17	-0.15829179137395144
2215	18	-0.10719571844968456
2215	19	-0.15143589271822933
2215	20	0.10067391027174821
2215	21	0.37387665173310225
2215	22	0.1592282885528391
2215	23	0.033367136198197755
2215	24	0.0907977301466083
2215	25	0.005503248478820666
2216	1	0.26590644993457957
2216	2	0.11074612845512277
2216	3	1.2846416410262123
2216	4	-0.15601377048842835
2216	5	-0.1516468386019909
2216	6	-0.5503601433786365
2216	7	-1.1897420352675425
2216	8	-6.4168415136996515
2216	9	-1.9096171021663189
2216	10	-0.6185322493787382
2216	11	-1.3157459470592556
2216	12	-0.015153014269809972
2216	13	0.5990755209194489
2216	14	1.1543488449268793
2216	15	0.6666082162635689
2216	16	0.1483556606283578
2216	17	0.04005622583431645
2216	18	0.19058515389892605
2216	19	0.3732455249632878
2216	20	0.5198914678033297
2216	21	0.3239139876620598
2216	22	0.19428555004169112
2216	23	-0.12216844778787816
2216	24	0.01037978586183641
2216	25	0.0235574109662835
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	161932.0
3	5.0
4	944.0
5	7626.0
6	13396.0
7	11771.0
8	9910.0
9	8076.0
10	7165.0
11	6491.0
12	6049.0
13	6076.0
14	5943.0
15	5712.0
16	5804.0
17	6131.0
18	7190.0
19	9544.0
20	13665.0
21	17390.0
22	21102.0
23	24155.0
24	27479.0
25	30106.0
26	33437.0
27	36359.0
28	39104.0
29	45296.0
30	51916.0
31	58295.0
32	72750.0
33	91954.0
34	116847.0
35	146673.0
36	185649.0
37	221989.0
38	303450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.81635080572828	17.922024159429046	12.095776653612738	22.16584838122994
2	14.536477393785843	22.200842316757466	40.06259418105947	23.200086108397226
3	16.108193926760762	29.144879789432775	30.446240880425375	24.300685403381088
4	10.317906773770966	17.043815345003953	37.407580586306054	35.230697294919025
5	11.711875345393274	41.342850407515755	32.632949842133534	14.312324404957433
6	29.62893682484871	37.85515364907942	18.167953168358736	14.347956357713135
7	27.68382221069506	34.96828344310196	20.480590681209286	16.867303664993695
8	26.183197513751182	35.30875043041417	20.256008140345923	18.25204391548873
9	27.79220177233952	17.428667445470403	19.147866576241558	35.63126420594852
10	12.926406854661618	33.089265836351025	32.105814142987754	21.878513165999596
11	33.21918148378275	26.061827408142342	21.92769475434912	18.79129635372579
12	21.92183724360485	29.58255550043511	29.295775891419957	19.199831364540092
13	26.74397495794044	24.685387883205475	26.513219861271054	22.057417297583033
14	20.112756853756007	24.030662977632932	29.033703450688602	26.822876717922455
15	22.693762059864937	31.235884750592195	24.28901630126805	21.78133688827482
16	21.59757828312369	31.666894741837297	25.193624783972346	21.541902191066665
17	20.8464538797734	30.791437246773683	26.28405709458546	22.078051778867454
18	20.448744334691057	29.33330611262971	29.13704116101451	21.080908391664728
19	21.65225706313354	28.942915016169767	29.293457659271226	20.111370261425463
20	22.13999841879664	28.185945092436814	29.13726559074808	20.536790898018463
21	22.914719323201812	27.674587733363936	28.864844523362056	20.545848420072197
22	22.381274261766354	28.041483999304738	28.6741729272485	20.903068811680413
23	21.71652715221118	28.30099147132712	28.787760910240088	21.19472046622161
24	21.37518222720546	28.14360552182919	29.302962316351948	21.1782499346134
25	21.405823601398794	27.95221632095907	29.40756492155783	21.234395156084304
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11448.0
1	11448.0
2	10781.5
3	10115.0
4	10115.0
5	10115.0
6	15021.5
7	19928.0
8	19928.0
9	19928.0
10	20030.5
11	20133.0
12	20133.0
13	20133.0
14	20944.0
15	21755.0
16	21755.0
17	21755.0
18	28500.5
19	35246.0
20	35246.0
21	35246.0
22	47399.0
23	59552.0
24	59552.0
25	59552.0
26	78071.5
27	96591.0
28	96591.0
29	96591.0
30	113089.5
31	129588.0
32	129588.0
33	129588.0
34	140366.5
35	151145.0
36	151145.0
37	151145.0
38	163382.5
39	175620.0
40	175620.0
41	175620.0
42	189131.0
43	202642.0
44	202642.0
45	202642.0
46	211803.5
47	220965.0
48	220965.0
49	220965.0
50	213476.5
51	205988.0
52	205988.0
53	205988.0
54	186670.0
55	167352.0
56	167352.0
57	167352.0
58	145374.5
59	123397.0
60	123397.0
61	123397.0
62	101388.0
63	79379.0
64	79379.0
65	79379.0
66	61554.5
67	43730.0
68	43730.0
69	43730.0
70	33145.5
71	22561.0
72	22561.0
73	22561.0
74	16596.5
75	10632.0
76	10632.0
77	10632.0
78	8145.0
79	5658.0
80	5658.0
81	5658.0
82	3992.5
83	2327.0
84	2327.0
85	2327.0
86	1705.0
87	1083.0
88	1083.0
89	1083.0
90	772.5
91	462.0
92	462.0
93	462.0
94	262.5
95	63.0
96	63.0
97	63.0
98	42.0
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31176731791517576
2	0.3142434085092779
3	0.3242578193565356
4	0.3267339099506378
5	0.332346381963936
6	0.2807336491357618
7	0.2881068966826439
8	0.28464036985090085
9	0.2861260242073621
10	0.39760512517738433
11	0.28475041832174985
12	0.2854657333822682
13	0.2789728736021781
14	0.2840901274966559
15	0.2788628251313291
16	0.2813389157254313
17	0.2762216618309534
18	0.27704702536232084
19	0.27776234042283926
20	0.4749692001842211
21	0.27968818866269646
22	0.28150398843170477
23	0.2764417587726514
24	0.27968818866269646
25	0.27308528041175734
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1817381.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.080484012653336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.73676106953859	51.146818957793236
2	8.816781601174286	10.77066575267164
3	3.018948155573823	5.5319644365466845
4	1.4359352223452293	3.508304735666544
5	0.7816459457208216	2.3871656345577965
6	0.4709297077990603	1.7258768692982405
7	0.3117164264915654	1.332785314335964
8	0.21922896288351568	1.071248893001372
9	0.1666263062209746	0.9159853889895946
>10	0.9277556009217671	10.312572715165482
>50	0.07469767192165813	3.1565320080079293
>100	0.03574142119505265	3.928665041362034
>500	0.0016621242241643779	0.674961085885587
>1k	0.0011080828161095853	1.49053201443122
>5k	3.6936093870319515E-4	1.3356525006147395
>10k+	9.234023467579879E-5	0.710268651671864
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	12593	0.6929201967006368	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7170	0.39452376799361283	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5706	0.3139682873321554	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5430	0.29878159835499546	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5375	0.29575526540664837	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4668	0.256853130961532	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3557	0.1957212054049206	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3486	0.1918144846897816	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3250	0.17882876512960133	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	2454	0.13502947373170512	No Hit
GTGGTATCAACGCAGAGTACATGGG	1931	0.10625179860469544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.1004847084898544E-4	0.0
5	5.502423542449272E-5	0.0	0.0	1.1004847084898544E-4	0.0
6	5.502423542449272E-5	0.0	0.0	1.1004847084898544E-4	0.0
7	1.1004847084898544E-4	0.0	0.0	1.1004847084898544E-4	0.0
8	1.1004847084898544E-4	0.0	0.0	1.1004847084898544E-4	0.0
9	1.1004847084898544E-4	0.0	0.0	1.1004847084898544E-4	0.0
10	1.1004847084898544E-4	0.0	0.0	1.1004847084898544E-4	0.0
11	1.1004847084898544E-4	0.0	0.0	1.1004847084898544E-4	0.0
12	1.1004847084898544E-4	0.0	0.0	1.1004847084898544E-4	0.0
13	1.1004847084898544E-4	0.0	0.0	1.1004847084898544E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	130	0.0	17.529846	3
CGCGCCA	35	0.0021719032	16.28401	10
TAACGCA	145	0.0	15.076967	4
TCACGCA	130	1.8189894E-12	13.891988	4
GGTATCA	2650	0.0	13.651782	1
TATAACG	155	0.0	13.477248	2
TAGCGCT	50	0.0014937269	13.307061	4
ATCACGC	115	3.8016879E-10	13.210899	3
TATCACG	130	1.4551915E-11	13.147385	2
AGAACCG	75	1.4708528E-5	12.673391	5
CGCATAT	155	1.8189894E-12	12.25746	8
GTCGAAA	55	0.0030528102	12.099336	16
AACCGAT	55	0.0030699167	12.090312	7
GACCGCG	55	0.0030699167	12.090312	7
ACCGAGT	65	8.027871E-4	11.69173	8
GTCGTGC	115	7.0045644E-8	11.573598	17
GGACCGA	85	5.324933E-5	11.176228	6
CGAGGTT	95	1.3524463E-5	11.00584	4
CGCAGAT	605	0.0	10.991192	8
TGGTATC	745	0.0	10.961057	2
>>END_MODULE