FastQCFastQC Report
Thu 26 May 2016
SRR1364325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364325_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817381
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT302631.665198436651423No Hit
TATCAACGCAGAGTACTTTTTTTTT205871.1327839346840316No Hit
GGTATCAACGCAGAGTACTTTTTTT199971.100319635783581No Hit
GTACTTTTTTTTTTTTTTTTTTTTT144350.7942748383525523No Hit
ACGCAGAGTACTTTTTTTTTTTTTT127940.7039800680209598No Hit
GAGTACTTTTTTTTTTTTTTTTTTT106800.5876588343335822No Hit
GTGGTATCAACGCAGAGTACTTTTT36870.20287435601010467No Hit
GCAGAGTACTTTTTTTTTTTTTTTT36100.1986374898824187No Hit
ATCAACGCAGAGTACTTTTTTTTTT34100.18763264279752018No Hit
GTGGTATCAACGCAGAGTACATGGG30420.16738372416130684No Hit
GTATAACGCAGAGTACTTTTTTTTT24470.13464430408373368No Hit
GGTATCAACGCAGAGTACATGGGAT18660.10267522330210341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG504.5760207E-617.09494814
TCTCGAC504.57842E-617.09400612
TTCGCGG1058.913048E-1114.47231216
TATCACG1450.014.41352
AGTCTCG602.5645584E-514.25245710
TATAACG3300.014.1057742
GGTATCA45000.014.08237651
GTGGTAT11850.013.8704441
TAACGCA3300.013.8190424
TCGCCAA551.9649006E-413.8144815
CTTCGCG1101.8735591E-1013.8144815
ATAACGC3250.013.7385613
CGCGGTT1400.013.56741818
ACGAAAT500.001503684313.29533812
TCGCGGT1153.8016879E-1013.21348617
CTCGACG655.4686192E-513.14995913
TGGTATC12150.013.1355352
CGTGCGC801.9960644E-613.06475210
ATCGCGT550.003055991112.09765719
GTCTCGA951.0403364E-611.99975711
Produced by FastQC (version 0.11.5)