##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364325_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1817381
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.473346535481554	31.0	30.0	34.0	26.0	34.0
2	29.470028023843103	31.0	30.0	34.0	25.0	34.0
3	29.505340927411478	31.0	30.0	34.0	25.0	34.0
4	32.81778449318002	35.0	33.0	37.0	26.0	37.0
5	32.419327592838265	35.0	32.0	37.0	25.0	37.0
6	32.2740047353857	35.0	32.0	37.0	23.0	37.0
7	31.987599188062383	35.0	32.0	37.0	19.0	37.0
8	31.85803362090833	35.0	32.0	37.0	17.0	37.0
9	32.52602893944638	37.0	32.0	39.0	11.0	39.0
10	32.684467373654726	37.0	32.0	39.0	17.0	39.0
11	32.61268385660464	37.0	32.0	39.0	15.0	39.0
12	32.5487671544932	37.0	32.0	39.0	15.0	39.0
13	32.582699499994774	37.0	32.0	39.0	15.0	39.0
14	33.47281720233677	38.0	32.0	41.0	13.0	41.0
15	33.50331163360902	38.0	32.0	41.0	13.0	41.0
16	33.48037808252645	38.0	32.0	41.0	11.0	41.0
17	33.492349705427756	38.0	32.0	41.0	11.0	41.0
18	33.46991302319106	38.0	32.0	41.0	10.0	41.0
19	33.40410678883514	39.0	32.0	41.0	10.0	41.0
20	33.295501603681345	39.0	32.0	41.0	10.0	41.0
21	33.24387346406725	38.0	32.0	41.0	2.0	41.0
22	33.18509657578681	38.0	32.0	41.0	2.0	41.0
23	32.915549903955196	38.0	32.0	41.0	2.0	41.0
24	32.82593798438522	38.0	32.0	41.0	2.0	41.0
25	32.667293759536385	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.12094311234918464
1101	2	0.35001333427804937
1101	3	-1.555844380113225
1101	4	-0.5730053042068874
1101	5	0.11394079495418197
1101	6	0.49568169082323976
1101	7	0.6358699665103913
1101	8	0.7383761341688917
1101	9	1.6075617705671306
1101	10	1.1830475558150084
1101	11	1.0962954465272858
1101	12	1.109287791785114
1101	13	0.9102356735977821
1101	14	1.1024273130618383
1101	15	0.9794235233444368
1101	16	0.9780406235488073
1101	17	1.0487389932416278
1101	18	1.0013391957718767
1101	19	1.0578287177280927
1101	20	1.2551283994006752
1101	21	1.1862296344687664
1101	22	1.2959898696209677
1101	23	1.6384725604586876
1101	24	1.6098214815305525
1101	25	1.6861340594463954
1102	1	0.8297208027478042
1102	2	0.907219221136792
1102	3	1.0372267646785787
1102	4	1.0136929636431447
1102	5	1.1548766257427374
1102	6	1.2248593791792288
1102	7	1.2768868641644318
1102	8	1.3781050261444214
1102	9	2.084776874792311
1102	10	1.555213790125059
1102	11	1.5134799623729833
1102	12	1.765469938180381
1102	13	1.4811626700129636
1102	14	1.5002427206175284
1102	15	1.7219109867047706
1102	16	1.6481430358944564
1102	17	1.7145957997943881
1102	18	1.708772452847036
1102	19	1.657578367927762
1102	20	2.018101140507575
1102	21	2.0856123773119037
1102	22	1.9790668103744906
1102	23	2.350144529451569
1102	24	2.253580992525009
1102	25	2.01553554341686
1103	1	-2.011662056249044
1103	2	-1.7776959660799498
1103	3	-1.9000216612407748
1103	4	-1.8198796924721954
1103	5	-4.38198119497936
1103	6	-7.798905182967804
1103	7	-10.170110849489763
1103	8	-10.05150365892792
1103	9	-10.514907928865842
1103	10	-8.946637212115657
1103	11	-10.935966321174448
1103	12	-10.454782852580955
1103	13	-10.78487343498658
1103	14	-10.773018693368218
1103	15	-10.873673222807646
1103	16	-5.303779699812079
1103	17	-3.2915351302566123
1103	18	-2.4183915911968086
1103	19	-2.1929720533479298
1103	20	-7.048803623811239
1103	21	-3.9191104983129144
1103	22	-9.340525424160987
1103	23	-10.69164021438645
1103	24	-12.022028868672002
1103	25	-12.646135339386376
1104	1	0.30934472298215
1104	2	0.5481771817349568
1104	3	0.6321825687023441
1104	4	0.8160251293248351
1104	5	0.8913359406065453
1104	6	0.8777371443846604
1104	7	0.7156470014439975
1104	8	0.8755891869531176
1104	9	1.2063091563849397
1104	10	1.1552798771055848
1104	11	0.9177979900733391
1104	12	0.8811041662095391
1104	13	0.7402191982830146
1104	14	0.8110102166651174
1104	15	0.8400115256512422
1104	16	0.4742635576514189
1104	17	0.4126051574348466
1104	18	0.597184758725767
1104	19	0.8699731018648293
1104	20	1.0235310163566993
1104	21	0.9308133586123901
1104	22	0.7710187369011479
1104	23	1.1317398721182315
1104	24	1.3128379994205304
1104	25	1.374563537487262
1105	1	0.48765200408506715
1105	2	0.5487515549886623
1105	3	0.1758253378381447
1105	4	0.5758100601348275
1105	5	0.5214812413795968
1105	6	0.7383154882397491
1105	7	0.9465508588377745
1105	8	0.6918834539806049
1105	9	0.8712189413915254
1105	10	0.6479256767296349
1105	11	1.0250358003018292
1105	12	1.1344692003663113
1105	13	1.1608528880780113
1105	14	0.8614936853744055
1105	15	0.7541083729634295
1105	16	0.821679054817686
1105	17	0.9227930840787479
1105	18	1.0462006709360736
1105	19	1.1324634468878614
1105	20	1.3823003623036811
1105	21	1.3367573387816094
1105	22	0.9582772993312787
1105	23	1.3570985270441795
1105	24	1.4032517754877034
1105	25	1.5482720438187556
1106	1	0.02022727535878488
1106	2	0.28892228650330054
1106	3	0.1620726312169012
1106	4	-1.1886386530080415
1106	5	-0.20726988656506506
1106	6	0.12855057726360286
1106	7	0.4920232424457396
1106	8	0.6003413634560566
1106	9	1.0299915031516775
1106	10	0.6825699189687029
1106	11	0.7167082877278403
1106	12	0.8801478439938961
1106	13	0.4580125698759332
1106	14	0.04615858164682152
1106	15	0.5428832307487497
1106	16	0.3862159648876613
1106	17	0.2701757045697377
1106	18	0.3562961993442286
1106	19	-0.9950148778445111
1106	20	0.24749896593303333
1106	21	-0.08524941270462705
1106	22	0.5280943028120078
1106	23	0.3203862057474325
1106	24	0.5252837297364081
1106	25	0.8735715400438195
1107	1	0.06462696099585585
1107	2	0.05294617718250194
1107	3	-0.14938082300453104
1107	4	0.06283939360003643
1107	5	0.21466271262734438
1107	6	0.10114772177104214
1107	7	-0.08049713084957588
1107	8	-0.15958157922792537
1107	9	0.6393698382854964
1107	10	0.44852664224085714
1107	11	0.37458449584232767
1107	12	0.2834595969788225
1107	13	0.2550610066381296
1107	14	0.36571454840004236
1107	15	0.2543492082898453
1107	16	0.01864109821144666
1107	17	-0.06483928388634297
1107	18	0.030374295418162944
1107	19	0.13481982311982676
1107	20	0.15421303382473184
1107	21	0.13212969240003503
1107	22	0.20171488362048962
1107	23	0.32097184034532944
1107	24	0.5234931961865215
1107	25	0.35830494830793214
1108	1	0.14423386650463854
1108	2	0.15727762101959541
1108	3	0.39837015519760755
1108	4	0.11905550813593635
1108	5	0.4235428120126272
1108	6	0.3670131074673435
1108	7	0.051452675883211896
1108	8	0.34444739199296137
1108	9	0.4719460741002237
1108	10	0.4586943736487754
1108	11	0.48420009231767125
1108	12	0.616777650132363
1108	13	0.22802045936953874
1108	14	0.39471512221867044
1108	15	-0.08660223146531365
1108	16	-0.5589031079315845
1108	17	-0.6118315824259213
1108	18	-0.710694356222966
1108	19	-0.49624964540721095
1108	20	0.13728738268297747
1108	21	0.07768162373564991
1108	22	0.08585223336781667
1108	23	-0.3326132020990187
1108	24	-0.010519609686660658
1108	25	-0.20711524706020157
1109	1	0.3517180094517123
1109	2	0.3251661485110944
1109	3	0.30538318925958663
1109	4	0.43582983515152307
1109	5	0.37519785479302215
1109	6	0.4235325635367744
1109	7	0.5914818362314733
1109	8	0.41825865742933743
1109	9	1.2530729641777825
1109	10	0.5216942370524364
1109	11	0.5138291243841593
1109	12	0.5496878152753553
1109	13	0.6372380890073757
1109	14	0.32985165916500847
1109	15	0.15347523251482187
1109	16	0.5067324213693212
1109	17	0.460405855660305
1109	18	0.2028366986570873
1109	19	0.5566705466987116
1109	20	0.9042743248651135
1109	21	0.7011850030102238
1109	22	0.5206170897041034
1109	23	0.8337339149264338
1109	24	0.6512765232512692
1109	25	0.7274768841989783
1110	1	0.01619868420790027
1110	2	0.057029108524265126
1110	3	-0.10265964638585956
1110	4	0.03978725988186227
1110	5	-0.039854785149394445
1110	6	0.034982732206671585
1110	7	0.1777767845583682
1110	8	-0.021540690482488856
1110	9	-3.2931396690254893
1110	10	-1.3661902958602106
1110	11	-0.5450899906445024
1110	12	-0.18246471707059442
1110	13	-0.5668493800251362
1110	14	-0.19738824265800048
1110	15	-0.12286436260082922
1110	16	-0.44651289473836897
1110	17	-0.3229248771060682
1110	18	-0.24968157551095516
1110	19	-0.24950457133193282
1110	20	0.16296101621642833
1110	21	-0.07132362193760144
1110	22	0.1386606528701151
1110	23	0.23140766789873624
1110	24	0.3312088734107448
1110	25	0.40488660463962844
1111	1	0.23774945477501674
1111	2	0.1920619897519309
1111	3	0.1851520103913593
1111	4	0.09543180237965032
1111	5	0.2768389520081129
1111	6	0.34655491658116944
1111	7	0.4088544880603209
1111	8	0.4213899099207943
1111	9	1.1723214111280456
1111	10	0.6704047098623818
1111	11	0.9158422716630525
1111	12	1.0748931640225194
1111	13	0.8199494792467661
1111	14	0.7507095415242375
1111	15	0.8879483113965918
1111	16	0.7278100290337619
1111	17	0.6722570747352137
1111	18	0.7537886032291681
1111	19	0.7822485177109897
1111	20	0.998922382909214
1111	21	0.8373538958579019
1111	22	0.8597944263358599
1111	23	1.2419997920824386
1111	24	1.0891820709337594
1111	25	1.304808938344486
1112	1	-0.1931666098044147
1112	2	-0.29121513930874343
1112	3	-0.17866956783786492
1112	4	-0.17253718033839505
1112	5	-0.12859259767061815
1112	6	-0.24776273799595927
1112	7	-0.14810524762935273
1112	8	-0.3887365360882029
1112	9	0.2023641386784547
1112	10	-0.19908189468738158
1112	11	0.08203597541223928
1112	12	-0.5097386879360641
1112	13	-0.3248624677160805
1112	14	-1.0629558453743329
1112	15	-0.5505229749750029
1112	16	-0.5801656068694925
1112	17	-0.5515336461534943
1112	18	-0.6903119828994164
1112	19	-0.31468297269950085
1112	20	-0.4097406462727591
1112	21	-0.663666795500788
1112	22	-0.4714138862654238
1112	23	0.026664436767568134
1112	24	-0.1012648825722593
1112	25	-0.29551409130596085
1113	1	-1.7292657341388313
1113	2	-1.7313919030149165
1113	3	-1.7706532774686323
1113	4	-1.7491399628235307
1113	5	-1.7153263902867621
1113	6	-1.4843137686646344
1113	7	-2.00231983713806
1113	8	-2.1648109298096543
1113	9	-11.02827891549401
1113	10	-4.5207584516580575
1113	11	-2.831516555787349
1113	12	-13.167260463104899
1113	13	-4.862565976007826
1113	14	-3.747776412547843
1113	15	-3.132972586836381
1113	16	-3.010822831644365
1113	17	-3.0075882786787176
1113	18	-3.191553366493739
1113	19	-4.113086741691507
1113	20	-14.61332852633123
1113	21	-17.17656786722539
1113	22	-12.28880043894609
1113	23	-18.761032762694338
1113	24	-19.79222345588378
1113	25	-16.666375256284773
1114	1	-0.29842093245004264
1114	2	-0.17279165132003627
1114	3	-0.21298673258981182
1114	4	-0.5991379330261069
1114	5	-0.36070048195241355
1114	6	-0.15329440439718667
1114	7	-0.3231496183258997
1114	8	-0.13405949880247192
1114	9	0.41782996283993157
1114	10	-0.30121556203844335
1114	11	-0.5074593966918286
1114	12	-0.3849562370666568
1114	13	-0.3800019358990312
1114	14	-0.5897769808911235
1114	15	-0.47785546752488983
1114	16	-0.4210042955342672
1114	17	-0.47509122188498765
1114	18	-0.5373496560240412
1114	19	-0.7733798444398516
1114	20	-1.3451516903794563
1114	21	-1.2410748294305627
1114	22	-1.3076978529841696
1114	23	-0.8816636237344078
1114	24	-0.5745276819220173
1114	25	-0.9640653468137401
1115	1	-0.060498227136029925
1115	2	-0.024945435539038385
1115	3	-0.03107967024796565
1115	4	0.07917469637347807
1115	5	-0.3135990326770539
1115	6	-0.19655699929021964
1115	7	-0.40478637931048667
1115	8	-0.06809446544613351
1115	9	0.35640829272261243
1115	10	-0.1596667927722777
1115	11	-0.04763077925451853
1115	12	-0.036152464349839875
1115	13	-0.1324461502997636
1115	14	-0.4228658927843867
1115	15	-0.3310156929677248
1115	16	-0.2501824643863486
1115	17	-0.23354532816517803
1115	18	-0.34845501604244333
1115	19	-0.39652511704164795
1115	20	-0.7199072689393802
1115	21	-0.13482339165738466
1115	22	-0.5474177747693076
1115	23	-0.29686376176063334
1115	24	0.12924791044053308
1115	25	0.06284976705789802
1116	1	-1.004290623289858
1116	2	-0.9742484640278661
1116	3	-0.9818437337539123
1116	4	-0.905239267090991
1116	5	-1.7143510356756941
1116	6	-1.766474211282702
1116	7	-2.8675053122245657
1116	8	-2.049149490859964
1116	9	-1.0720850462331732
1116	10	-2.104331948838716
1116	11	-1.7796751567154239
1116	12	-2.95231588682681
1116	13	-2.684254922713656
1116	14	-2.0570688624457
1116	15	-3.1211406320144377
1116	16	-3.392380943027103
1116	17	-4.052752250028707
1116	18	-3.320151651768274
1116	19	-2.957557032267559
1116	20	-3.306834134543685
1116	21	-2.576029936955436
1116	22	-2.359377221096583
1116	23	-2.5776746703593396
1116	24	-3.0203651772055196
1116	25	-2.6409615267829167
1201	1	0.728167394206757
1201	2	0.8168260002284597
1201	3	0.4091825318252589
1201	4	0.7697971753817896
1201	5	0.8378735105277713
1201	6	0.9627249370586455
1201	7	1.2557849318513803
1201	8	1.1532291948539033
1201	9	1.835427960718654
1201	10	1.3877252472378672
1201	11	1.4387795368802117
1201	12	1.6169316198366062
1201	13	1.4368013490658456
1201	14	1.4649296195190047
1201	15	1.710801286349259
1201	16	1.574721288174885
1201	17	1.546987787013606
1201	18	1.523652353112169
1201	19	1.655727584325227
1201	20	1.6836604195692502
1201	21	1.7963135306212692
1201	22	1.7707325606054383
1201	23	2.0772130581823305
1201	24	1.8846404655378137
1201	25	2.1406127739547642
1202	1	0.9096003754389628
1202	2	0.9998200641084125
1202	3	0.9139380127025589
1202	4	1.0381659354014445
1202	5	1.1714981541515073
1202	6	1.1794038345011515
1202	7	1.185447329889648
1202	8	1.2194755584827597
1202	9	1.928686312010072
1202	10	1.6050323333512964
1202	11	1.6998410029759228
1202	12	1.6788258552979443
1202	13	1.5813239269278156
1202	14	1.6457315327778872
1202	15	1.5429263141720853
1202	16	1.4827720179778936
1202	17	1.4699973690816108
1202	18	1.4917828571177765
1202	19	1.4893334498898483
1202	20	1.6999472561748448
1202	21	1.7986413840162072
1202	22	1.8120732088691014
1202	23	2.0324367762230153
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2211	7	0.2498255841912922
2211	8	0.05405400069231803
2211	9	0.6044725267634732
2211	10	-0.057573467035382464
2211	11	0.2554088988686374
2211	12	0.3980390011437933
2211	13	0.19292925491703983
2211	14	0.17317899242995338
2211	15	0.1965654387244271
2211	16	0.25670677340856685
2211	17	0.2347430890380977
2211	18	0.23884900035172052
2211	19	-0.020444943736933396
2211	20	0.4356541385210235
2211	21	0.6163609013883402
2211	22	0.5064088189362508
2211	23	0.9389770575943786
2211	24	0.8947228863704311
2211	25	1.0994133518685558
2212	1	-0.3700740924636641
2212	2	-0.3285892750683459
2212	3	-0.36939300881357795
2212	4	-0.3228382615173757
2212	5	-0.5645840886570639
2212	6	-0.47494843490920147
2212	7	-0.4151821902865187
2212	8	-0.5026309552298471
2212	9	0.1167754062683315
2212	10	-0.10486037362429101
2212	11	-0.1362931586585674
2212	12	-0.1424357333475399
2212	13	-0.3970985243113745
2212	14	-0.3309597026819304
2212	15	-0.22365426818702616
2212	16	-0.16129284137714706
2212	17	-0.333540523014058
2212	18	-0.2187966889825077
2212	19	-0.10856141995837021
2212	20	0.21666240041282947
2212	21	0.17105183992420336
2212	22	-0.2942340284599325
2212	23	0.1550947377508436
2212	24	0.23686560994773487
2212	25	0.3131821233438359
2213	1	-0.38991902922483845
2213	2	-0.42455730023365135
2213	3	-0.5558669951857453
2213	4	-0.5308717126949958
2213	5	-0.5238368891034746
2213	6	-0.2645695687446157
2213	7	-0.40803090123604946
2213	8	-0.30566299112900097
2213	9	0.0974409143971613
2213	10	-0.2963251142977015
2213	11	-0.25752567558371453
2213	12	-0.2713832890992194
2213	13	-0.6411276179832406
2213	14	-0.533656929916944
2213	15	-0.7101129715760308
2213	16	-0.7601445596668981
2213	17	-0.8101799940676457
2213	18	-0.9287799856602916
2213	19	-1.0822041780341465
2213	20	-0.5155365509653578
2213	21	-0.6913746592166774
2213	22	-0.45956857903395587
2213	23	-0.7948597338037438
2213	24	-0.553792944929814
2213	25	-0.1731598490921442
2214	1	-1.1199221733149436
2214	2	-0.9121414655330824
2214	3	-1.028185385234476
2214	4	-0.7710118904025123
2214	5	-1.1466643117688236
2214	6	-0.9949184520390872
2214	7	-0.8263980335380339
2214	8	-0.9347839079633893
2214	9	-10.732175750166068
2214	10	-4.093280882263855
2214	11	-1.9229286173006983
2214	12	-1.2378010855348869
2214	13	-1.2393884047585786
2214	14	-1.2336375561222752
2214	15	-1.3326665041384018
2214	16	-1.4264837538498902
2214	17	-1.3369354259261357
2214	18	-1.740086642938973
2214	19	-1.8138185575966759
2214	20	-1.5154813079736869
2214	21	-2.100532596605003
2214	22	-1.7935944978500054
2214	23	-2.260721114856395
2214	24	-2.525908629732566
2214	25	-9.956161060231079
2215	1	-0.8480692188354624
2215	2	-0.5282492800126093
2215	3	-0.5440766121439928
2215	4	-0.6137953050827605
2215	5	-0.6282803588262702
2215	6	-0.6726170206484063
2215	7	-0.713624542506448
2215	8	-0.8398895239384387
2215	9	-0.1161901248299344
2215	10	-0.14061128575805526
2215	11	-0.0012798739586585839
2215	12	0.015046506573902718
2215	13	-0.15760876376513266
2215	14	-0.3218604804257623
2215	15	-0.7349250905697815
2215	16	-0.5252267583526589
2215	17	-0.6098862824877855
2215	18	-0.5405998148386217
2215	19	-0.520745421881216
2215	20	-0.26096413511092464
2215	21	-0.5048357696972872
2215	22	-0.4565878393292806
2215	23	-0.17613539905979536
2215	24	0.1428910588313883
2215	25	0.2259971870860511
2216	1	-0.9519384207326951
2216	2	-0.9615014660644761
2216	3	-0.9477091884321496
2216	4	-0.8187353034673102
2216	5	-1.7291194033127688
2216	6	-2.177642119730905
2216	7	-1.557479246271157
2216	8	-2.212717487952805
2216	9	-0.9557185640381469
2216	10	-1.5089630280389272
2216	11	-1.0321829808327934
2216	12	-1.9972274883636807
2216	13	-1.9687772334964464
2216	14	-1.9833393103917025
2216	15	-1.788347675688371
2216	16	-2.8724815706999998
2216	17	-3.310681498581186
2216	18	-2.5697027539652098
2216	19	-3.8117260770645736
2216	20	-2.70577392976843
2216	21	-2.8608831847934084
2216	22	-2.6989534001640116
2216	23	-2.4581830370468083
2216	24	-2.586223570138774
2216	25	-2.6489204642710114
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71361.0
3	3.0
4	944.0
5	9759.0
6	14121.0
7	11161.0
8	8762.0
9	6959.0
10	5929.0
11	5310.0
12	4789.0
13	4329.0
14	4397.0
15	4454.0
16	4544.0
17	4847.0
18	5513.0
19	6997.0
20	9982.0
21	12922.0
22	15547.0
23	17947.0
24	20449.0
25	22878.0
26	25205.0
27	27492.0
28	30495.0
29	35131.0
30	42311.0
31	52648.0
32	65047.0
33	80507.0
34	100739.0
35	126152.0
36	165271.0
37	244873.0
38	547606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59616177394117	18.7930855130303	12.28605505809131	23.324697654937225
2	16.270789141362034	21.62929092004589	38.0601478522358	24.03977208635627
3	19.298847020267658	27.609115990179255	29.221548846745144	23.870488142807936
4	11.768149882903982	16.86045103977743	36.27858067299396	35.092818404324625
5	11.923240985108883	39.992294376821484	33.445982205514476	14.638482432555158
6	30.14927377203805	37.62176713819848	17.524122302577926	14.704836787185545
7	26.881373058363568	34.25408800324046	21.107921524426754	17.756617413969217
8	27.374831783494002	34.02071161430054	19.706966158554202	18.897490443651254
9	27.295870765535202	14.978843845174486	19.93907731973233	37.78620806955798
10	15.330546871946632	31.572556162720094	31.023245649525688	22.07365131580758
11	33.851424029206115	23.637046227965776	22.39412398725976	20.117405755568353
12	23.064943669482137	28.12503267351632	29.605094812353588	19.204928844647952
13	28.630204449971526	22.3869279679087	26.837724885339085	22.145142696780688
14	21.70313056292464	21.78165464043181	29.531546374053047	26.9836684225905
15	23.5136771090702	28.94930307549236	25.95431608951889	21.58270372591855
16	21.584002262813208	29.08788046711244	27.149741333816124	22.178375936258224
17	20.8511036391752	28.19571091312315	28.558417759483152	22.394767688218494
18	20.241759511748146	25.963969963045407	32.31454601988338	21.479724505323055
19	21.5044733341735	25.796199550822983	32.58943779129503	20.10988932370848
20	21.685613200149024	25.06407077970713	32.667430141250136	20.582885878893705
21	22.382895172731065	24.497281008662693	32.5193785225548	20.600445296051436
22	21.829595849926893	24.927898147776194	32.47089924670693	20.771606755589993
23	20.97672212372239	25.03078807568749	32.888048063515335	21.104441737074787
24	21.00166840183392	25.116766117796214	32.9346483920172	20.946917088352663
25	20.968402871104335	24.845836766526695	33.07244735647783	21.11331300589114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2185.0
1	2185.0
2	2926.5
3	3668.0
4	3668.0
5	3668.0
6	10784.0
7	17900.0
8	17900.0
9	17900.0
10	17855.0
11	17810.0
12	17810.0
13	17810.0
14	15912.5
15	14015.0
16	14015.0
17	14015.0
18	21843.5
19	29672.0
20	29672.0
21	29672.0
22	45476.0
23	61280.0
24	61280.0
25	61280.0
26	83473.5
27	105667.0
28	105667.0
29	105667.0
30	120682.0
31	135697.0
32	135697.0
33	135697.0
34	145267.5
35	154838.0
36	154838.0
37	154838.0
38	161043.0
39	167248.0
40	167248.0
41	167248.0
42	179487.5
43	191727.0
44	191727.0
45	191727.0
46	205070.5
47	218414.0
48	218414.0
49	218414.0
50	214019.5
51	209625.0
52	209625.0
53	209625.0
54	191612.5
55	173600.0
56	173600.0
57	173600.0
58	152833.5
59	132067.0
60	132067.0
61	132067.0
62	109436.0
63	86805.0
64	86805.0
65	86805.0
66	67112.5
67	47420.0
68	47420.0
69	47420.0
70	36434.5
71	25449.0
72	25449.0
73	25449.0
74	18296.5
75	11144.0
76	11144.0
77	11144.0
78	8829.0
79	6514.0
80	6514.0
81	6514.0
82	4676.5
83	2839.0
84	2839.0
85	2839.0
86	2006.0
87	1173.0
88	1173.0
89	1173.0
90	880.5
91	588.0
92	588.0
93	588.0
94	310.5
95	33.0
96	33.0
97	33.0
98	18.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027512117712246358
2	0.0014306301210368106
3	1.6507270627347815E-4
4	0.003796672244289997
5	0.02894274783328317
6	0.010069435082682168
7	0.0196436520465439
8	0.02894274783328317
9	0.035875801496769257
10	0.04071793421412461
11	0.04264378245398186
12	0.008803877667918835
13	0.0036315995380165195
14	0.005722520484147242
15	0.006107690132118691
16	0.009574216963861733
17	0.011610113674567962
18	0.015736931331404917
19	0.015406785918857961
20	0.013756058856123179
21	0.008968950374192314
22	0.008803877667918835
23	0.006547884015514634
24	0.004016769185987969
25	0.09755796940762558
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1817381.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.97102614271919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.35113487561323	34.17997965107656
2	12.943370815922348	11.900400763073495
3	5.022997963900144	6.927371121398364
4	2.4109140625918397	4.433287735970351
5	1.347100806733439	3.0963803201610514
6	0.8397043601925067	2.3161242656739005
7	0.5693908747264088	1.8322837951231443
8	0.40215275275202017	1.4789899768103683
9	0.2769203320932224	1.1457280643497205
>10	1.620642883796222	13.885434023593143
>50	0.13515203345605334	4.276544827850014
>100	0.0745365311818725	5.937157459289584
>500	0.0037086583652731074	1.1336540298338555
>1k	0.0015552438305984	1.4754056497527213
>5k	0.0	0.0
>10k+	7.178048448915692E-4	5.981258316043797
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	30263	1.665198436651423	No Hit
TATCAACGCAGAGTACTTTTTTTTT	20587	1.1327839346840316	No Hit
GGTATCAACGCAGAGTACTTTTTTT	19997	1.100319635783581	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	14435	0.7942748383525523	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	12794	0.7039800680209598	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	10680	0.5876588343335822	No Hit
GTGGTATCAACGCAGAGTACTTTTT	3687	0.20287435601010467	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	3610	0.1986374898824187	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	3410	0.18763264279752018	No Hit
GTGGTATCAACGCAGAGTACATGGG	3042	0.16738372416130684	No Hit
GTATAACGCAGAGTACTTTTTTTTT	2447	0.13464430408373368	No Hit
GGTATCAACGCAGAGTACATGGGAT	1866	0.10267522330210341	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.1004847084898544E-4	0.0	0.0	5.502423542449272E-5	0.0
8	1.1004847084898544E-4	0.0	0.0	5.502423542449272E-5	0.0
9	1.6507270627347815E-4	0.0	0.0	5.502423542449272E-5	0.0
10	1.6507270627347815E-4	0.0	0.0	1.1004847084898544E-4	0.0
11	2.2009694169797088E-4	0.0	0.0	1.1004847084898544E-4	0.0
12	2.751211771224636E-4	0.0	0.0	1.1004847084898544E-4	0.0
13	2.751211771224636E-4	0.0	0.0	1.1004847084898544E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGG	50	4.5760207E-6	17.094948	14
TCTCGAC	50	4.57842E-6	17.094006	12
TTCGCGG	105	8.913048E-11	14.472312	16
TATCACG	145	0.0	14.4135	2
AGTCTCG	60	2.5645584E-5	14.252457	10
TATAACG	330	0.0	14.105774	2
GGTATCA	4500	0.0	14.0823765	1
GTGGTAT	1185	0.0	13.870444	1
TAACGCA	330	0.0	13.819042	4
TCGCCAA	55	1.9649006E-4	13.81448	15
CTTCGCG	110	1.8735591E-10	13.81448	15
ATAACGC	325	0.0	13.738561	3
CGCGGTT	140	0.0	13.567418	18
ACGAAAT	50	0.0015036843	13.295338	12
TCGCGGT	115	3.8016879E-10	13.213486	17
CTCGACG	65	5.4686192E-5	13.149959	13
TGGTATC	1215	0.0	13.135535	2
CGTGCGC	80	1.9960644E-6	13.064752	10
ATCGCGT	55	0.0030559911	12.097657	19
GTCTCGA	95	1.0403364E-6	11.999757	11
>>END_MODULE