FastQCFastQC Report
Thu 26 May 2016
SRR1364324_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364324_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688236
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA173521.027818385581163No Hit
GTATCAACGCAGAGTACTTTTTTTT94330.5587488952966292No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA75910.44964092697940333No Hit
GTACTTTTTTTTTTTTTTTTTTTTT68180.4038534896779834No Hit
TATCAACGCAGAGTACTTTTTTTTT56090.33224027920267074No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN46280.274132289561412No Hit
GGTATCAACGCAGAGTACTTTTTTT40820.24179083966933534No Hit
GAACAAAAAAAAAAAAAAAAAAAAA38400.22745635088933064No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30250.17918110975005863No Hit
AAACAAAAAAAAAAAAAAAAAAAAA25740.15246683520550444No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22040.13055046806252205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2550.015.6386122
TATCACG953.074092E-1014.9919022
ATAACGC2700.014.76983
GTATAAC3600.013.7144061
ATCACGC1257.2759576E-1213.6726153
TAACGCA3150.013.2753074
GTCTAGG1153.8016879E-1013.2098631
GTATCAC1700.012.845621
TAAGGCT1850.012.3294024
ACGAAAT853.9902134E-612.28127412
AAATCCG701.0847038E-412.22186419
CGCATAT701.0916562E-412.2135028
TTGCGCG1251.3969839E-912.16754318
TGGACGC802.8537594E-511.8802455
TGCGCGC1302.590241E-911.69956119
TTTGCGC1552.5465852E-1111.65273217
TTATACC1800.011.6158944
CGAAACC1157.0132955E-811.57239218
GAACAAA11250.011.56231
GGTATCA21800.011.49803451
Produced by FastQC (version 0.11.5)