##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364324_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1688236
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.3745951395421	30.0	22.0	31.0	2.0	34.0
2	24.332951672633445	30.0	20.0	31.0	2.0	34.0
3	23.903836904319064	30.0	16.0	31.0	2.0	34.0
4	27.29304137573183	33.0	23.0	35.0	2.0	37.0
5	27.97814108927899	35.0	27.0	37.0	2.0	37.0
6	27.99001798326774	35.0	27.0	37.0	2.0	37.0
7	27.558377501723694	35.0	25.0	37.0	2.0	37.0
8	27.081457213327994	35.0	22.0	37.0	2.0	37.0
9	27.962231583735925	35.0	21.0	39.0	2.0	39.0
10	27.343785466013045	34.0	17.0	39.0	2.0	39.0
11	27.91901902340668	35.0	22.0	39.0	2.0	39.0
12	27.511135883845622	34.0	18.0	39.0	2.0	39.0
13	27.100093825744743	34.0	17.0	39.0	2.0	39.0
14	27.317507149474363	34.0	17.0	39.0	2.0	41.0
15	27.955201168557004	34.0	17.0	39.0	2.0	41.0
16	28.268684591490764	35.0	19.0	40.0	2.0	41.0
17	28.415951916675155	36.0	22.0	40.0	2.0	41.0
18	28.238503384597887	36.0	19.0	40.0	2.0	41.0
19	28.196238559064017	35.0	18.0	40.0	2.0	41.0
20	28.045982907602966	35.0	18.0	40.0	2.0	41.0
21	27.945447792844128	35.0	18.0	40.0	2.0	41.0
22	27.975440045112176	35.0	18.0	40.0	2.0	41.0
23	27.927439054729316	35.0	18.0	40.0	2.0	41.0
24	27.787600193337898	35.0	16.0	40.0	2.0	41.0
25	27.588558708616567	35.0	15.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2806104952951287
1101	2	0.30919385937846044
1101	3	1.0218084961427785
1101	4	0.5356451997630778
1101	5	0.031052139079289276
1101	6	-0.222287118745907
1101	7	0.032842553555603615
1101	8	0.4107486857157241
1101	9	0.25484304998360585
1101	10	0.46989937583557406
1101	11	-0.05265054092887311
1101	12	0.22998538162305948
1101	13	0.032462085941496355
1101	14	0.49942683035355273
1101	15	-0.23550314370677228
1101	16	-0.6361165033071963
1101	17	-1.1377284165623927
1101	18	-6.804536412325199
1101	19	-8.05859782518679
1101	20	-9.410291077194906
1101	21	-11.167068855285905
1101	22	-11.220856934417412
1101	23	-12.15846727651451
1101	24	-11.738910784875612
1101	25	-12.066838168982441
1102	1	1.445677281609239
1102	2	1.574441329501969
1102	3	2.4800684140468476
1102	4	1.742031584207293
1102	5	1.20355667133256
1102	6	1.1563094288594264
1102	7	1.4205263447627274
1102	8	1.769427874942604
1102	9	1.7639760708592576
1102	10	-2.0857557848699386
1102	11	0.2839100396846028
1102	12	1.355192288837678
1102	13	1.844532431362861
1102	14	2.381386643364003
1102	15	2.109938531195759
1102	16	1.9061307667302145
1102	17	1.3625483692060278
1102	18	1.6832277164817455
1102	19	1.321789492992547
1102	20	1.797272783617931
1102	21	1.9903382714171016
1102	22	1.766874756188848
1102	23	1.8467501200621967
1102	24	1.9788080517146796
1102	25	2.0662008963123206
1103	1	0.33210812196913153
1103	2	0.5145700527469934
1103	3	-1.9947831550795314
1103	4	0.22801006380948152
1103	5	-2.2296702241754325
1103	6	-6.435460765223294
1103	7	-7.550399498008645
1103	8	-1.8728512249925373
1103	9	-5.726249943210494
1103	10	-3.3866896104782143
1103	11	-7.599384211009738
1103	12	-1.7129570234741855
1103	13	0.5886916556972075
1103	14	1.114473007680722
1103	15	0.7650575253583121
1103	16	0.8357687118149499
1103	17	0.574174206988129
1103	18	0.9661369616047288
1103	19	-4.824901787421229
1103	20	-0.7922563953140305
1103	21	-5.962646145420031
1103	22	-1.574110458931976
1103	23	-0.892643516951555
1103	24	-6.226045679430026
1103	25	-3.3979262303653215
1104	1	1.40017605431132
1104	2	1.411303083262311
1104	3	2.1950832383980803
1104	4	1.7060038651632077
1104	5	1.2394533026417065
1104	6	1.3102147876204562
1104	7	1.3511712553620683
1104	8	1.7524504003316856
1104	9	1.9252004948210377
1104	10	2.2336922683159024
1104	11	1.7587813713541678
1104	12	1.8937202058330413
1104	13	2.142727262341797
1104	14	2.5309080411639577
1104	15	1.7107310694590083
1104	16	0.9700071512894297
1104	17	0.9235230812968922
1104	18	0.8430311435315652
1104	19	1.4815528898951236
1104	20	-8.346256848955171
1104	21	-3.345322577925849
1104	22	-1.00365337781977
1104	23	0.024997180852302847
1104	24	0.5394293841173976
1104	25	1.1409868889866601
1105	1	1.2823661199051486
1105	2	1.231712582273424
1105	3	1.9377704511582863
1105	4	1.1459093872815593
1105	5	1.112377706107793
1105	6	1.1696533271972989
1105	7	1.5363336747926368
1105	8	1.4539002943286405
1105	9	1.4916608468365204
1105	10	1.8695654061483182
1105	11	1.335187512416475
1105	12	1.7503614033117358
1105	13	1.6697374073311977
1105	14	2.4518425753908453
1105	15	1.7207736129629438
1105	16	1.139105485120762
1105	17	0.6361395779318286
1105	18	1.0390414450355294
1105	19	1.189408498096487
1105	20	1.1708900328478755
1105	21	1.0578560905620797
1105	22	1.0440067294638489
1105	23	1.142260737147744
1105	24	1.20583139888668
1105	25	1.2475960721229242
1106	1	0.9234299771271068
1106	2	1.1021034318573903
1106	3	1.5828649210121242
1106	4	1.2395365594055576
1106	5	0.5549630930511569
1106	6	0.41652417920843376
1106	7	0.963520968578333
1106	8	0.9844514885701905
1106	9	1.1878570225330023
1106	10	-2.244758666029533
1106	11	-0.1778774673697825
1106	12	0.4126060768199764
1106	13	1.3206381134848186
1106	14	0.5225679187969163
1106	15	1.1502257560747218
1106	16	0.6312740821429337
1106	17	-5.461625153828397
1106	18	-1.7016105524462866
1106	19	-0.39994989572637607
1106	20	0.15617913107983838
1106	21	0.6074529534499504
1106	22	0.7194900768292385
1106	23	0.37599834532729304
1106	24	0.6859233278010528
1106	25	0.8321289564305374
1107	1	0.77745092711287
1107	2	0.9704598781893665
1107	3	1.4345444194462118
1107	4	0.950382566333527
1107	5	0.40637330231375657
1107	6	0.6470182121400079
1107	7	0.5464258513722982
1107	8	0.7114062076553189
1107	9	0.942897593853111
1107	10	-1.9744101322912755
1107	11	-0.09614197576875938
1107	12	0.28535834264516424
1107	13	0.9836150044405017
1107	14	1.3851568909901424
1107	15	1.0942361480268907
1107	16	-5.358644182068893
1107	17	-1.9671429126781632
1107	18	-0.3883261142760297
1107	19	0.43007821948668123
1107	20	0.8067426216369213
1107	21	0.7169545905383075
1107	22	0.47956617771184185
1107	23	0.3200529268020986
1107	24	0.4870310316066906
1107	25	0.6945260452603748
1108	1	-0.4837643141868071
1108	2	-2.8530484731266874
1108	3	-0.11572481851246508
1108	4	-0.7771436209990696
1108	5	-1.0344338318562478
1108	6	-0.6826169812215284
1108	7	-0.2588967372872304
1108	8	-0.022607363381148105
1108	9	-0.46575552425548494
1108	10	-3.2394570529874933
1108	11	-1.671946672807291
1108	12	-5.776016110774865
1108	13	-2.1121967969545814
1108	14	-5.6060410419437865
1108	15	-2.6486245947539935
1108	16	-7.028959743271653
1108	17	-3.2597132996378555
1108	18	-1.9138268205709252
1108	19	-0.5975385617015014
1108	20	-0.5267959749539344
1108	21	-0.02397803815643229
1108	22	-0.3444366983399121
1108	23	-0.24489736636352433
1108	24	-0.03281547051810918
1108	25	-0.19167485127274375
1109	1	-7.995865717877649
1109	2	-5.559752729313633
1109	3	-4.746233065372852
1109	4	-4.532878478197215
1109	5	-2.3310653614434216
1109	6	-1.5005011761805171
1109	7	-1.086843808281678
1109	8	-0.6424854524474455
1109	9	-5.44968584404473
1109	10	-1.5968004082499903
1109	11	-1.2466033857410075
1109	12	-5.6034543330919035
1109	13	-7.504888814835393
1109	14	-7.688609171492114
1109	15	-4.01517706190506
1109	16	-2.4330625324531105
1109	17	-1.805469535183498
1109	18	-1.2677262670012688
1109	19	-0.9905993928705534
1109	20	-0.6874717936108716
1109	21	-0.773877601744374
1109	22	-0.9368993263043528
1109	23	-1.2406792556347277
1109	24	-0.9025461289860743
1109	25	-0.8727605000351382
1110	1	-2.571136351126924
1110	2	-2.412510790672755
1110	3	-1.7233469608505771
1110	4	-2.5144304579395858
1110	5	-5.293155274231246
1110	6	-4.0211077564419
1110	7	-8.573992812525276
1110	8	-9.410351436257933
1110	9	-10.815481202762708
1110	10	-8.946868055291269
1110	11	-11.292587420956032
1110	12	-6.881439767719115
1110	13	-5.005866308686262
1110	14	-4.578559580308298
1110	15	-9.30247979618279
1110	16	-10.738766122025822
1110	17	-11.459262062893956
1110	18	-8.665394458992075
1110	19	-11.528319779828553
1110	20	-12.427898034475957
1110	21	-13.227634922197419
1110	22	-13.956813899459359
1110	23	-14.671688082280047
1110	24	-15.350227076324868
1110	25	-15.80394210303627
1111	1	-0.36644016423203496
1111	2	0.2257372478978823
1111	3	-2.2302066974337684
1111	4	-2.610985600462829
1111	5	-0.8007136030231194
1111	6	-0.22329052859178944
1111	7	0.17543583062225565
1111	8	0.4078925356580392
1111	9	-3.9368455125713524
1111	10	-3.910536112775503
1111	11	-1.041304873664238
1111	12	-5.3080949686412495
1111	13	-1.046654231832722
1111	14	0.18442706190967328
1111	15	0.42147806937349586
1111	16	0.3279924439920876
1111	17	0.22056433619712834
1111	18	0.5488285640983115
1111	19	0.6795094380150388
1111	20	0.640216263716578
1111	21	0.7172002705941161
1111	22	0.2995063145100758
1111	23	0.23227289625839376
1111	24	0.44459001159005496
1111	25	0.5767065270280654
1112	1	-0.1105501714033359
1112	2	0.33696585179768235
1112	3	-2.3491699924950886
1112	4	-0.33121612158780067
1112	5	-0.309438365838826
1112	6	-0.03981042501758836
1112	7	0.3524527365898642
1112	8	-3.1203688569365227
1112	9	-0.5942023461037671
1112	10	-2.769695496325447
1112	11	-0.5728267937289289
1112	12	0.47735745450732026
1112	13	0.7916709036772254
1112	14	1.4140685639188817
1112	15	0.9580934864790507
1112	16	0.702574499984582
1112	17	0.5619417538095846
1112	18	0.5111657651191734
1112	19	0.6641176091392502
1112	20	0.7230262480595329
1112	21	0.6627659573380988
1112	22	0.7589709604649109
1112	23	0.5399374063374012
1112	24	0.6893827489764384
1112	25	0.8339402324948431
1113	1	-1.9846993598311755
1113	2	-1.796285996989976
1113	3	-3.506340623595392
1113	4	-2.509064169677952
1113	5	-2.5927075800391854
1113	6	-2.4164905755213972
1113	7	-2.4535293136205283
1113	8	-7.834751432229904
1113	9	-9.258052908298968
1113	10	-4.561300376193717
1113	11	-9.537744378925144
1113	12	-8.121586599966026
1113	13	-4.000872083077432
1113	14	-2.6234514303871883
1113	15	-7.732790394297357
1113	16	-4.406752144766166
1113	17	-3.506754641516043
1113	18	-3.012182821593104
1113	19	-2.812254994286633
1113	20	-7.078031409926922
1113	21	-4.033000574454519
1113	22	-3.253105092015563
1113	23	-3.196379103327029
1113	24	-8.159198344290008
1113	25	-5.558455482716667
1114	1	-3.9189114152844873
1114	2	-3.4937972116845515
1114	3	-0.6738045918329263
1114	4	-0.45380366970224983
1114	5	-0.44902795581333166
1114	6	-0.47333845925623663
1114	7	-0.36715873320329706
1114	8	0.058097786914633076
1114	9	-0.22874784958953853
1114	10	-2.649782361897227
1114	11	-0.8615817589865458
1114	12	-4.027673173393488
1114	13	-1.067152354300834
1114	14	-5.268305877069057
1114	15	-1.7232254166844854
1114	16	-0.8532459547618778
1114	17	-0.6108088354842991
1114	18	-0.17250646827581306
1114	19	0.029297165928724667
1114	20	0.3496927096429552
1114	21	0.3973299183158865
1114	22	0.4338316640798858
1114	23	0.047677055721958794
1114	24	0.11350995954950349
1114	25	0.15618785513142086
1115	1	0.5389113394805172
1115	2	0.7363956762002566
1115	3	1.3739562500676392
1115	4	0.35924950672796285
1115	5	0.14134981274980163
1115	6	-0.4619603618256889
1115	7	-2.250146279137496
1115	8	-0.2681672802172166
1115	9	-0.09928847901316473
1115	10	0.77287302795364
1115	11	-0.19734108523731564
1115	12	0.5004342661592531
1115	13	0.7005921872151006
1115	14	1.4885109723811638
1115	15	0.5661099851771887
1115	16	0.2935147012571129
1115	17	-0.0407993984195123
1115	18	0.23212555769041288
1115	19	0.69553249200791
1115	20	0.5632313229663772
1115	21	0.7924605358716867
1115	22	0.40895212964175087
1115	23	0.3892165841546884
1115	24	0.2843172575462738
1115	25	0.5110214911180933
1116	1	0.519729744207325
1116	2	0.6370420782063917
1116	3	1.413361114844843
1116	4	0.9156510771665403
1116	5	-0.048995185108029915
1116	6	-0.9387287390795045
1116	7	-4.369327511331484
1116	8	-0.8518609091454294
1116	9	-1.1071348626232833
1116	10	-0.11883666359847567
1116	11	-1.903772307144063
1116	12	-0.37385198064262326
1116	13	-6.057351985726783
1116	14	-1.6120848405894783
1116	15	-0.9855019654537216
1116	16	-0.5120646158151132
1116	17	-0.09102329726080782
1116	18	-0.0037292396750849832
1116	19	0.37367923893928534
1116	20	0.5128024054022973
1116	21	0.5949717126597918
1116	22	0.40370381977543346
1116	23	0.4013669086264713
1116	24	0.6753516217824895
1116	25	0.5683537545780446
1201	1	2.1307415997240433
1201	2	2.211516798671269
1201	3	2.848672864342152
1201	4	2.4818248250718575
1201	5	2.0410637077551677
1201	6	1.9691916666949787
1201	7	2.223439200344064
1201	8	2.265056506704642
1201	9	2.7396945305602856
1201	10	2.8092162630343225
1201	11	2.5674273872585616
1201	12	2.9194649833482273
1201	13	2.8959075493811675
1201	14	3.371738926143305
1201	15	2.968656249280187
1201	16	2.51633946181261
1201	17	2.4344626931286726
1201	18	2.439974044883872
1201	19	2.4924363673317202
1201	20	2.5124127823670115
1201	21	2.326609280164604
1201	22	1.5847804785502255
1201	23	1.1177261053847403
1201	24	1.6383969459315573
1201	25	1.7436944704576014
1202	1	1.7498343891776997
1202	2	1.8531934344594028
1202	3	2.607104281934731
1202	4	2.073042961245509
1202	5	1.514333466633886
1202	6	1.4040844815200337
1202	7	1.6912329650372087
1202	8	1.9057152874438508
1202	9	2.0972945399171152
1202	10	-1.2676425895005536
1202	11	0.8735158038941648
1202	12	1.8504264305137816
1202	13	2.3576811543064586
1202	14	2.68268599618127
1202	15	2.3389599533548733
1202	16	2.0062316873373796
1202	17	1.5981343248126976
1202	18	1.9114781521419246
1202	19	2.025604526642528
1202	20	2.0752052020159937
1202	21	2.264786581877054
1202	22	1.9021705650499108
1202	23	1.7697864221433903
1202	24	1.8058269039187955
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1203	1	2.0359732374440966
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2211	15	-1.608206035157
2211	16	-0.8257140306210751
2211	17	-0.27023801306244977
2211	18	0.13262499304453002
2211	19	0.40760421962610494
2211	20	0.5311013865268208
2211	21	0.5436411140353741
2211	22	0.3139728201102585
2211	23	0.24344850470884083
2211	24	0.48097853790249445
2211	25	0.5046440153106566
2212	1	0.6364383793816089
2212	2	0.7934978438916609
2212	3	1.4738737952417331
2212	4	0.9372011058429699
2212	5	0.7452722367456239
2212	6	0.588069568395813
2212	7	0.8871894225305788
2212	8	1.1000209045833174
2212	9	0.8551129263378918
2212	10	1.5938047185807847
2212	11	0.8876299333096398
2212	12	1.1911616683529616
2212	13	1.3517412729739888
2212	14	-4.0963182199527886
2212	15	-1.0064101590356174
2212	16	-0.2439225284172366
2212	17	0.12526491438847032
2212	18	0.5087243303557081
2212	19	0.8919867388715943
2212	20	1.0951705861351861
2212	21	1.198245871628128
2212	22	0.9419536222403764
2212	23	0.7974251919298965
2212	24	0.8364114927508659
2212	25	0.9643533312007264
2213	1	0.3375073160617177
2213	2	0.5142021887872481
2213	3	1.1159739737306928
2213	4	0.508859929812914
2213	5	0.09365597552765692
2213	6	-0.2204986505839308
2213	7	-0.03594599192577874
2213	8	-5.289865511533112
2213	9	-1.8026198894190664
2213	10	-0.23283070211644485
2213	11	-0.11153953134829564
2213	12	0.3310262414148575
2213	13	-7.22287485094478
2213	14	-2.4009946307020655
2213	15	-1.0041876594412713
2213	16	-0.5570321056605181
2213	17	-0.4131626353200808
2213	18	-0.07732419867058837
2213	19	0.22521177226026268
2213	20	0.5599290019520531
2213	21	0.3379002520966914
2213	22	0.06864196553830482
2213	23	-0.03413078245756296
2213	24	0.11644569273338945
2213	25	0.15342780977580972
2214	1	-1.0369436721902652
2214	2	-0.6424429963750953
2214	3	-6.226529186825562
2214	4	-2.339828670600273
2214	5	-1.4057020461183
2214	6	-1.1035371014488433
2214	7	-0.6089095144813612
2214	8	-6.036514088511929
2214	9	-1.9537487476103017
2214	10	-0.3637394005839596
2214	11	-0.6924072196711997
2214	12	-0.3123224064837551
2214	13	-5.859982556535108
2214	14	-1.4615330044802413
2214	15	-0.8941349321124825
2214	16	-0.8754342517553013
2214	17	-0.605873313375028
2214	18	-0.6052141804781215
2214	19	-0.19063171082690644
2214	20	-0.06612323202161363
2214	21	0.04506870203656632
2214	22	-0.11574087803728617
2214	23	-0.25876893730940154
2214	24	-0.017509473619789162
2214	25	0.07450209055654256
2215	1	0.19887534167752818
2215	2	0.5360423168663146
2215	3	-2.0998401351365104
2215	4	-0.45792926918867494
2215	5	-0.22095562722553908
2215	6	-0.7471332974840621
2215	7	0.08503288007079135
2215	8	0.3767298040002558
2215	9	0.5022358955694628
2215	10	1.3640435887843942
2215	11	0.5328220903142089
2215	12	0.339707632131649
2215	13	0.7913898289836183
2215	14	1.050894157337165
2215	15	0.5100298732718365
2215	16	0.5816418473939677
2215	17	0.3400532081612475
2215	18	0.47397171943948635
2215	19	0.559692648643793
2215	20	0.5703839599733804
2215	21	0.7275666918516279
2215	22	0.5905241983497405
2215	23	0.29421755226245594
2215	24	0.3894646272567499
2215	25	0.35682909056647105
2216	1	-0.2043116065331141
2216	2	-0.4798077931781073
2216	3	0.7070523974669207
2216	4	-0.8882887990742709
2216	5	-0.8768647523289772
2216	6	-1.1117145210700272
2216	7	-1.608557779752097
2216	8	-6.105412812028614
2216	9	-2.457614526958686
2216	10	-0.9856278692993854
2216	11	-1.4982215763929645
2216	12	-0.5374607059371641
2216	13	0.002977960003029523
2216	14	0.8954612323544744
2216	15	0.3259321121598546
2216	16	-0.347847019891109
2216	17	-0.23890004851712732
2216	18	-0.14809609338662
2216	19	0.1076707149867886
2216	20	0.10359620586335438
2216	21	-0.21428529502405524
2216	22	-0.28998332729996434
2216	23	-0.5604813735265175
2216	24	-0.4546084052049082
2216	25	-0.41008433215263906
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	229919.0
3	4.0
4	1346.0
5	9545.0
6	14667.0
7	12886.0
8	10799.0
9	8922.0
10	8202.0
11	7740.0
12	7416.0
13	7136.0
14	7314.0
15	7247.0
16	7026.0
17	7333.0
18	8449.0
19	11030.0
20	15197.0
21	19547.0
22	23653.0
23	26888.0
24	30190.0
25	32641.0
26	35256.0
27	37246.0
28	39097.0
29	44791.0
30	50227.0
31	55998.0
32	67695.0
33	83677.0
34	102482.0
35	124703.0
36	154250.0
37	174712.0
38	203005.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.870269566721284	18.830088631270026	12.551609454239864	21.748032347768824
2	13.820797554179862	23.37395039891973	40.586694909613065	22.218557137287345
3	15.54553117023768	31.642614878544318	29.079224081667103	23.7326298695509
4	10.20192107073033	19.158159914069564	37.2332729593156	33.4066460558845
5	11.466504656985716	41.87500445843667	32.75048686128436	13.908004023293252
6	27.971993488515785	40.127080882615054	18.1844484844164	13.716477144452762
7	26.150971310757054	35.721835850116015	21.599016063242892	16.52817677588404
8	24.806424013404158	36.784368947036945	20.62380426128599	17.785402778272907
9	26.38214691011069	19.404124605631377	19.401569750515428	34.81215873374251
10	13.180246464148965	33.20009017706469	31.735307711388185	21.88435564739816
11	31.196749285237267	28.220234855704675	22.600583693190327	17.98243216586773
12	21.82905910795313	30.862178883506537	28.84990728269065	18.45885472584968
13	26.145538628621267	26.35793396079568	26.271431134601013	21.22509627598204
14	20.590599624621902	25.259713754892005	28.321023728197265	25.82866289228883
15	21.918077597376943	32.74757355959713	23.739222338995276	21.595126504030652
16	21.061906207612783	32.419705277567026	25.01825453927563	21.50013397554456
17	20.217351784808308	32.25796103042782	26.050014792706065	21.474672392057805
18	19.773711569879698	30.787167681568395	28.82893212535274	20.610188623199168
19	21.37984748497544	30.070387893852583	28.522365147298906	20.02739947387307
20	21.572634289520472	29.65676193220002	28.58805300805788	20.18255077022163
21	22.57045544065061	28.954799322967133	28.342641842128245	20.132103394254013
22	21.9228703558477	29.27746804880251	28.049978849709362	20.749682745640428
23	21.446725085400267	29.656081984256645	28.316233476904795	20.58095945343829
24	21.23187243421795	29.606941498684066	28.231474385251985	20.929711681845998
25	21.060726402378133	29.363817227328816	28.763165070333514	20.812291299959547
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23884.0
1	23884.0
2	22130.0
3	20376.0
4	20376.0
5	20376.0
6	23336.5
7	26297.0
8	26297.0
9	26297.0
10	23347.0
11	20397.0
12	20397.0
13	20397.0
14	20606.0
15	20815.0
16	20815.0
17	20815.0
18	27793.0
19	34771.0
20	34771.0
21	34771.0
22	45101.5
23	55432.0
24	55432.0
25	55432.0
26	72823.0
27	90214.0
28	90214.0
29	90214.0
30	107064.0
31	123914.0
32	123914.0
33	123914.0
34	133297.5
35	142681.0
36	142681.0
37	142681.0
38	152456.5
39	162232.0
40	162232.0
41	162232.0
42	173331.5
43	184431.0
44	184431.0
45	184431.0
46	190677.0
47	196923.0
48	196923.0
49	196923.0
50	188682.0
51	180441.0
52	180441.0
53	180441.0
54	164392.0
55	148343.0
56	148343.0
57	148343.0
58	128756.5
59	109170.0
60	109170.0
61	109170.0
62	90631.0
63	72092.0
64	72092.0
65	72092.0
66	55917.0
67	39742.0
68	39742.0
69	39742.0
70	29940.0
71	20138.0
72	20138.0
73	20138.0
74	14570.5
75	9003.0
76	9003.0
77	9003.0
78	6603.0
79	4203.0
80	4203.0
81	4203.0
82	2925.0
83	1647.0
84	1647.0
85	1647.0
86	1198.0
87	749.0
88	749.0
89	749.0
90	511.5
91	274.0
92	274.0
93	274.0
94	159.0
95	44.0
96	44.0
97	44.0
98	33.5
99	23.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3344911493416797
2	0.3371566534536641
3	0.3491218052452382
4	0.3521427099054872
5	0.35729601785532356
6	0.29948419533761866
7	0.3080730419206793
8	0.3065922063029103
9	0.30599987205580265
10	0.4204980820217079
11	0.30564447150753804
12	0.30582217178167037
13	0.29936572848819715
14	0.30404516904034745
15	0.2967002243762128
16	0.3016758320519169
17	0.2946270545113361
18	0.29533785560786524
19	0.2956932561561298
20	0.48956425523445773
21	0.2967002243762128
22	0.2987733942410895
23	0.29533785560786524
24	0.2977071925962958
25	0.2901845476580288
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1688236.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.378243425866394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.51079651460797	53.09880727439202
2	7.131730722138851	8.754662086223368
3	2.496599737808099	4.597107193324191
4	1.149143406233104	2.8212961487601897
5	0.6538568471334064	2.006629236451187
6	0.400754239481663	1.4758554758912084
7	0.275385152160175	1.1831859833609535
8	0.21044542770731703	1.0333416551744221
9	0.14092419141849538	0.7784711392930023
>10	0.8982953139983513	10.429512746981901
>50	0.08459584461535354	3.582846180029706
>100	0.04373013321514191	4.9514152736903325
>500	0.002068206819054939	0.8158197142504745
>1k	0.0011818324680313937	1.641855309629308
>5k	3.939441560104645E-4	1.7802835213686075
>10k+	9.848603900261612E-5	1.0489110611791816
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	17352	1.027818385581163	No Hit
GTATCAACGCAGAGTACTTTTTTTT	9433	0.5587488952966292	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	7591	0.44964092697940333	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6818	0.4038534896779834	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5609	0.33224027920267074	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4628	0.274132289561412	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4082	0.24179083966933534	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3840	0.22745635088933064	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3025	0.17918110975005863	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	2574	0.15246683520550444	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2204	0.13055046806252205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1846684942152638E-4
2	0.0	0.0	0.0	0.0	1.1846684942152638E-4
3	0.0	0.0	0.0	0.0	1.1846684942152638E-4
4	0.0	0.0	0.0	0.0	1.1846684942152638E-4
5	0.0	0.0	0.0	0.0	1.1846684942152638E-4
6	0.0	0.0	0.0	0.0	1.1846684942152638E-4
7	0.0	0.0	0.0	0.0	1.1846684942152638E-4
8	0.0	0.0	0.0	0.0	1.1846684942152638E-4
9	0.0	0.0	0.0	0.0	1.1846684942152638E-4
10	0.0	0.0	0.0	0.0	1.1846684942152638E-4
11	0.0	0.0	0.0	0.0	1.1846684942152638E-4
12	0.0	0.0	0.0	0.0	2.9616712355381595E-4
13	0.0	0.0	0.0	5.923342471076319E-5	2.9616712355381595E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	255	0.0	15.638612	2
TATCACG	95	3.074092E-10	14.991902	2
ATAACGC	270	0.0	14.7698	3
GTATAAC	360	0.0	13.714406	1
ATCACGC	125	7.2759576E-12	13.672615	3
TAACGCA	315	0.0	13.275307	4
GTCTAGG	115	3.8016879E-10	13.209863	1
GTATCAC	170	0.0	12.84562	1
TAAGGCT	185	0.0	12.329402	4
ACGAAAT	85	3.9902134E-6	12.281274	12
AAATCCG	70	1.0847038E-4	12.221864	19
CGCATAT	70	1.0916562E-4	12.213502	8
TTGCGCG	125	1.3969839E-9	12.167543	18
TGGACGC	80	2.8537594E-5	11.880245	5
TGCGCGC	130	2.590241E-9	11.699561	19
TTTGCGC	155	2.5465852E-11	11.652732	17
TTATACC	180	0.0	11.615894	4
CGAAACC	115	7.0132955E-8	11.572392	18
GAACAAA	1125	0.0	11.5623	1
GGTATCA	2180	0.0	11.4980345	1
>>END_MODULE