FastQCFastQC Report
Thu 26 May 2016
SRR1364324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364324_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688236
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT292001.729616001554285No Hit
GTATCAACGCAGAGTACTTTTTTTT250921.4862850928424698No Hit
TATCAACGCAGAGTACTTTTTTTTT188511.116609289222597No Hit
GGTATCAACGCAGAGTACTTTTTTT170141.0077974880289249No Hit
GAGTACTTTTTTTTTTTTTTTTTTT122820.7275049222975936No Hit
ACGCAGAGTACTTTTTTTTTTTTTT77150.45698587164353804No Hit
GTGGTATCAACGCAGAGTACTTTTT34670.20536228347221597No Hit
ATCAACGCAGAGTACTTTTTTTTTT30620.1813727464643569No Hit
GCAGAGTACTTTTTTTTTTTTTTTT30280.17935881002419093No Hit
GTATAACGCAGAGTACTTTTTTTTT28100.16644592343724457No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA25210.149327463695834No Hit
GTGGTATCAACGCAGAGTACATGGG20320.12036231901227079No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT18880.1118327058539209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG4550.017.119652
TAACGCA4700.016.7783744
ATAACGC4800.016.6252843
ACCGTAT350.00216876616.2874418
GTATAAC5200.015.1637211
CGCGCCA456.7618734E-414.77803110
GTACCGT400.005275638314.2515116
GCGAATC400.005284981314.2477115
CGGTGTT759.691666E-713.93109614
TATCACG1257.2759576E-1213.6790192
CGGTTTG1600.013.06040214
CGCCAGT604.0999483E-412.66388112
GGTATCA39800.012.6509711
GTGCACG1155.329639E-912.39041611
TGACTAG1001.4423676E-712.35021210
ACGGCCC1201.00371835E-811.87309315
GAATGCG1850.011.80996311
CTAGAAC907.460083E-611.611313
AAACGCA1157.05204E-811.5678165
CTACGCA1251.828812E-811.401214
Produced by FastQC (version 0.11.5)