##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364324_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1688236
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.465133429212504	31.0	30.0	34.0	26.0	34.0
2	29.47101412361779	31.0	30.0	34.0	25.0	34.0
3	29.517463198273227	31.0	30.0	34.0	25.0	34.0
4	32.84057560672797	35.0	33.0	37.0	26.0	37.0
5	32.4392786316605	35.0	32.0	37.0	25.0	37.0
6	32.282493087459336	35.0	32.0	37.0	23.0	37.0
7	32.002420277733684	35.0	32.0	37.0	19.0	37.0
8	31.880564091750205	35.0	32.0	37.0	17.0	37.0
9	32.58259923375642	37.0	32.0	39.0	11.0	39.0
10	32.72823527042428	37.0	32.0	39.0	17.0	39.0
11	32.64523443404832	37.0	32.0	39.0	15.0	39.0
12	32.61720873148067	37.0	32.0	39.0	15.0	39.0
13	32.6325116867547	37.0	32.0	39.0	15.0	39.0
14	33.51945225667502	38.0	32.0	41.0	13.0	41.0
15	33.55191513508775	38.0	32.0	41.0	14.0	41.0
16	33.520091385327646	38.0	32.0	41.0	11.0	41.0
17	33.528729395653215	38.0	32.0	41.0	11.0	41.0
18	33.490258470972066	38.0	32.0	41.0	10.0	41.0
19	33.407653313873176	39.0	32.0	41.0	10.0	41.0
20	33.31199903331051	39.0	32.0	41.0	10.0	41.0
21	33.23881199074063	38.0	32.0	41.0	2.0	41.0
22	33.14054077747424	38.0	32.0	41.0	2.0	41.0
23	32.8708894964922	38.0	32.0	41.0	2.0	41.0
24	32.78554893984016	38.0	32.0	41.0	2.0	41.0
25	32.61486071852514	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.4145993489355817
1101	2	0.5833666172073819
1101	3	-1.5335931051444405
1101	4	-0.14004650103773741
1101	5	0.5381962695002613
1101	6	0.7851517394478194
1101	7	0.9201025145614992
1101	8	1.0241028301962416
1101	9	1.642984264298967
1101	10	1.3541362410920854
1101	11	1.261182610030943
1101	12	1.3685157100618994
1101	13	1.2196865177455294
1101	14	1.2573066785351728
1101	15	1.3055021974788232
1101	16	1.3047900988020587
1101	17	1.2318852815103156
1101	18	1.2179907843150346
1101	19	1.3539704649721642
1101	20	1.5832044267398047
1101	21	1.3677061152087688
1101	22	1.5145301215360405
1101	23	1.66464052583202
1101	24	1.7101759713393676
1101	25	1.9302522233958115
1102	1	0.7326883448065331
1102	2	0.7275544192312822
1102	3	0.7399097354718265
1102	4	0.7776756703208463
1102	5	0.6693936332143409
1102	6	1.001588206048602
1102	7	1.163349608765671
1102	8	1.3369131910181693
1102	9	1.8959740356551293
1102	10	1.565718194538718
1102	11	1.498810922820077
1102	12	1.3962870540947847
1102	13	1.2406988927675116
1102	14	1.3405155138521891
1102	15	1.4290277751516385
1102	16	1.2610076227062876
1102	17	1.3992576053587626
1102	18	1.4412216897349381
1102	19	1.408856506844728
1102	20	1.6610309460299248
1102	21	1.7885605434778995
1102	22	1.6349834681276079
1102	23	1.8882907025747357
1102	24	2.045129799956527
1102	25	1.8552899669096874
1103	1	-2.04015171572998
1103	2	-2.024532760000035
1103	3	-2.0987380052190225
1103	4	-1.9745902911952307
1103	5	-3.9567559784539057
1103	6	-8.143419194706443
1103	7	-9.605206329300817
1103	8	-9.859265025815638
1103	9	-10.840132501955676
1103	10	-8.882135586498716
1103	11	-10.993419115929942
1103	12	-10.811388110966998
1103	13	-11.736226550862021
1103	14	-11.716479446982017
1103	15	-11.188252572101767
1103	16	-5.646615986533845
1103	17	-3.5985728924043805
1103	18	-2.8203700546812307
1103	19	-2.666494821133451
1103	20	-7.568049032017271
1103	21	-4.3373620562659845
1103	22	-10.567942740523794
1103	23	-11.340074977878952
1103	24	-12.686108038690382
1103	25	-13.470194408350174
1104	1	0.396092872172197
1104	2	0.5215699855332296
1104	3	0.5327974193832254
1104	4	0.6131972206165983
1104	5	0.6094400564547016
1104	6	0.6496233563302809
1104	7	0.4324944299203608
1104	8	0.639086549415552
1104	9	0.9683246410927779
1104	10	0.8881177745887499
1104	11	0.7644436484185491
1104	12	0.8752262112035822
1104	13	0.8470563546508885
1104	14	0.6883500735059016
1104	15	0.7055652700562263
1104	16	0.3554655734560086
1104	17	0.23160211096444527
1104	18	0.4931428435416194
1104	19	0.7227761474238648
1104	20	0.8910240837189676
1104	21	0.7349870097103448
1104	22	0.47406007673833983
1104	23	0.8431408021369009
1104	24	1.175785854479642
1104	25	1.140906240557527
1105	1	0.47623401985079994
1105	2	0.5085024423850122
1105	3	0.12804791382695768
1105	4	0.3893358640621969
1105	5	0.4105413498341335
1105	6	0.5443459698389077
1105	7	0.7258795553454362
1105	8	0.5353686171152177
1105	9	0.7204454134210039
1105	10	0.47727273823468863
1105	11	1.0263721924062565
1105	12	1.2485362096384378
1105	13	0.8869817968045339
1105	14	0.6438003374463719
1105	15	0.5840900491572611
1105	16	0.8280314162869331
1105	17	0.840275863472236
1105	18	0.9312868653967428
1105	19	0.9817565261891019
1105	20	1.3136617809628888
1105	21	1.157305986389673
1105	22	0.9427785070395629
1105	23	1.3374849349750733
1105	24	1.3364352476299928
1105	25	1.3500096966836495
1106	1	-0.13798917323784465
1106	2	0.11755501328480733
1106	3	-0.027626724563223348
1106	4	-1.3627882854815745
1106	5	-0.285949878236039
1106	6	-0.015011134551983218
1106	7	0.3387260901866469
1106	8	0.4495902932250786
1106	9	0.8803281509609135
1106	10	0.47524681306725114
1106	11	0.6215186187834831
1106	12	0.7979563444978481
1106	13	0.4801455319096988
1106	14	-0.08766787047073876
1106	15	0.5475756113116788
1106	16	0.2267366748938926
1106	17	0.23385676774329056
1106	18	0.2262649071288294
1106	19	-1.0640516821830985
1106	20	0.12378230492736009
1106	21	0.001341605051550232
1106	22	0.49755661096777715
1106	23	0.4373119993935788
1106	24	0.3898055952456829
1106	25	0.7044077974262137
1107	1	0.10324143082403481
1107	2	-0.11492428585156844
1107	3	-0.11867048701932958
1107	4	-0.12488553802024427
1107	5	0.14720560956883588
1107	6	-0.1560593456177557
1107	7	-0.19988873139444507
1107	8	-0.1765648624169458
1107	9	0.6250962894253718
1107	10	0.5041636655869439
1107	11	0.22866278932538364
1107	12	0.2126044298162526
1107	13	0.12878188476155117
1107	14	0.2472664680506611
1107	15	0.14653789896470926
1107	16	-0.2129991963218245
1107	17	-0.10509970705978589
1107	18	-0.01897834284744704
1107	19	0.22475205702160395
1107	20	0.12407146455534246
1107	21	0.31440361273874373
1107	22	0.2744099017526267
1107	23	0.5042362899886257
1107	24	0.7242817269719808
1107	25	0.3047645759210411
1108	1	0.06997532564406583
1108	2	0.12070122287004992
1108	3	0.22529231833194885
1108	4	-0.04190269022029014
1108	5	0.2962387131492932
1108	6	0.2957954770631872
1108	7	-0.11159136941180492
1108	8	0.3170990394068838
1108	9	0.536028155037684
1108	10	0.4980349010469709
1108	11	0.5944991454115893
1108	12	0.6244863034734607
1108	13	0.01784478404886869
1108	14	0.33930056777902706
1108	15	-0.07971323087051019
1108	16	-0.6497279802642026
1108	17	-0.6663570362003028
1108	18	-0.8004071348347281
1108	19	-0.4878379748740542
1108	20	2.0558841840312425E-4
1108	21	-0.07513029769785362
1108	22	0.04672547833534679
1108	23	-0.2075933213953789
1108	24	0.05074968460848339
1108	25	-0.018896983417192814
1109	1	0.10212561494610384
1109	2	0.1713624128561051
1109	3	0.0441414605824022
1109	4	0.02628754982310255
1109	5	0.12062165762576882
1109	6	0.23133956263719568
1109	7	0.23602061919126527
1109	8	0.004979683208290453
1109	9	0.888681709087777
1109	10	0.18622883942352786
1109	11	0.3029459023100074
1109	12	0.2528137286539831
1109	13	0.643738711831702
1109	14	0.09610354090082751
1109	15	-0.21309555473229835
1109	16	0.231328932828319
1109	17	0.07972659946229044
1109	18	-0.0185314154411671
1109	19	0.16806855317943104
1109	20	0.44246051665136577
1109	21	0.37726925459691074
1109	22	0.12200593891042644
1109	23	0.3986523735364287
1109	24	0.0742389089508606
1109	25	-0.0028475580650209054
1110	1	-0.20582080732097552
1110	2	-0.11228475937549476
1110	3	-0.22828031181863295
1110	4	-0.09948475910019283
1110	5	-0.08192328052639652
1110	6	0.04831481532001192
1110	7	-0.08710792711105952
1110	8	-0.3184135417667804
1110	9	-3.362627638827483
1110	10	-1.5374550881860216
1110	11	-0.6309062086203205
1110	12	-0.21320121070921516
1110	13	-0.3651664492775666
1110	14	-0.21338531436529706
1110	15	-0.06885261411339627
1110	16	-0.5028516959389009
1110	17	-0.438821646340962
1110	18	-0.28006987271130157
1110	19	-0.5036699653774122
1110	20	-0.05225000214796438
1110	21	-0.11329920943862959
1110	22	0.07323445440294307
1110	23	0.1478941620817551
1110	24	0.24149432844782837
1110	25	0.3700350298931312
1111	1	-0.029646743664148545
1111	2	0.10534493365168274
1111	3	-0.05123913768521149
1111	4	-0.2190519922672962
1111	5	0.14210292704449046
1111	6	0.07021785527306434
1111	7	0.10992989020348531
1111	8	0.18370397696699214
1111	9	0.9084600043537776
1111	10	0.31383499095118594
1111	11	0.5188007335059837
1111	12	0.65158563738823
1111	13	0.3256168883838626
1111	14	0.20012790002719072
1111	15	0.19065982894225897
1111	16	0.24460254708422013
1111	17	0.34394096682467534
1111	18	0.26030467945884794
1111	19	0.3026373862049283
1111	20	0.4737335776162368
1111	21	0.4734621634108862
1111	22	0.4478255029672198
1111	23	0.7958641062255367
1111	24	0.7466450405391072
1111	25	0.9471571307253015
1112	1	-0.2580605212597362
1112	2	-0.26093042321385695
1112	3	-0.35406496919289054
1112	4	-0.3104963971827388
1112	5	-0.24719568204059073
1112	6	-0.25770848454480344
1112	7	-0.11568635536984573
1112	8	-0.48501436819837096
1112	9	0.28353692520015983
1112	10	0.02005760873701945
1112	11	0.056066532695624005
1112	12	-0.4524611343161702
1112	13	-0.3839639167686357
1112	14	-1.0831897516434807
1112	15	-0.38246602771899774
1112	16	-0.5419043020341121
1112	17	-0.5411982744399921
1112	18	-0.704382377335854
1112	19	-0.20625862337613654
1112	20	-0.24925275806818092
1112	21	-0.47649493599170256
1112	22	-0.19668823349905296
1112	23	0.023754569025392414
1112	24	-0.04925904321751773
1112	25	-0.1263676406253893
1113	1	-2.5836981315862957
1113	2	-2.762722886999345
1113	3	-2.6321882416821722
1113	4	-2.97751286944062
1113	5	-2.7130660905746353
1113	6	-2.8180033915288014
1113	7	-3.0732970886397837
1113	8	-3.480658298571896
1113	9	-12.197962354764009
1113	10	-5.752001147899428
1113	11	-4.191463645394844
1113	12	-13.569026667744723
1113	13	-5.9863992486674285
1113	14	-5.1056074559091655
1113	15	-4.935263571623526
1113	16	-4.486246147541337
1113	17	-4.385968609498807
1113	18	-4.581993945429943
1113	19	-5.093935173968351
1113	20	-15.760142129418835
1113	21	-18.751571100317207
1113	22	-13.412036475578791
1113	23	-19.258721027084544
1113	24	-20.65358771348713
1113	25	-17.28793180141038
1114	1	-0.42749164597470113
1114	2	-0.2068855407457839
1114	3	-0.23982078426420372
1114	4	-0.6809747923038074
1114	5	-0.3936818369989261
1114	6	-0.48111143082196506
1114	7	-0.5344772358306678
1114	8	-0.39276055461134973
1114	9	0.28706499789191753
1114	10	-0.5600027241899923
1114	11	-0.833975842629787
1114	12	-0.6182920867612118
1114	13	-0.5759747067108414
1114	14	-0.6268820960141426
1114	15	-0.42814543646400693
1114	16	-0.5637543195302754
1114	17	-0.5688595179366089
1114	18	-0.5206918787143948
1114	19	-0.9262546242528629
1114	20	-1.1771724064529572
1114	21	-1.424807229065916
1114	22	-1.5386103890264913
1114	23	-1.1810063103202921
1114	24	-0.7839306728987054
1114	25	-0.9244917057341908
1115	1	-0.2545288365891132
1115	2	-0.14998790430155395
1115	3	-0.23767599681264073
1115	4	0.009242832129615408
1115	5	-0.5568930127714076
1115	6	-0.2307396676636948
1115	7	-0.3952733809326894
1115	8	-0.0705705836374726
1115	9	0.38498526899304863
1115	10	0.0032148859448426492
1115	11	0.055907194961186235
1115	12	-0.10783624793484137
1115	13	-0.10473630643630827
1115	14	-0.20187101461230839
1115	15	-0.3822505596463017
1115	16	-0.16666122145629458
1115	17	0.01000074201162704
1115	18	-0.18058220858270602
1115	19	-0.05610363121627415
1115	20	-0.5625442411041419
1115	21	-0.02953720032576257
1115	22	-0.482066642511775
1115	23	-0.31908772645195427
1115	24	0.17038892902874636
1115	25	0.22242266005216038
1116	1	-0.48893748949265614
1116	2	-0.39763841878478345
1116	3	-0.3895888190756871
1116	4	-0.27403023005616944
1116	5	-0.9297544157578557
1116	6	-0.9669508877792481
1116	7	-1.9017170685587068
1116	8	-1.0817404732371898
1116	9	-0.08202644394201286
1116	10	-1.1486950316845856
1116	11	-1.0519597631370488
1116	12	-1.8521322736698913
1116	13	-1.465950854777887
1116	14	-0.996478078197157
1116	15	-1.951521230350405
1116	16	-2.344970022738206
1116	17	-2.955474419740156
1116	18	-2.270244012408593
1116	19	-1.7360884060950674
1116	20	-2.0561166499894163
1116	21	-1.3748781680044537
1116	22	-1.1895832291799877
1116	23	-1.2414563099347546
1116	24	-1.7038064269462083
1116	25	-1.2261902563694278
1201	1	0.8390203805590062
1201	2	0.9138453831243822
1201	3	0.46758499079344773
1201	4	0.9189432092557155
1201	5	1.1700391970663375
1201	6	1.0965968880261272
1201	7	1.3790960056705401
1201	8	1.3596292948206639
1201	9	2.1094585019145526
1201	10	1.7816358482901364
1201	11	1.8254316827239379
1201	12	1.800700119651907
1201	13	1.6773164360210302
1201	14	1.8478383076917098
1201	15	1.757690065585379
1201	16	1.7111882614496494
1201	17	1.6602519970693308
1201	18	1.5800432341440782
1201	19	1.6437506105459647
1201	20	1.8906403612845892
1201	21	1.9170919341132802
1201	22	1.8778889671104935
1201	23	2.126707634069021
1201	24	2.147022560408409
1201	25	2.3150033574080595
1202	1	0.9221615040322604
1202	2	0.9804324167786795
1202	3	0.9755420441480638
1202	4	1.0668542821930984
1202	5	1.197063188635596
1202	6	1.099206303695155
1202	7	1.3734258272939925
1202	8	1.173826663332477
1202	9	1.86735160194295
1202	10	1.5216505073221924
1202	11	1.6265623295477312
1202	12	1.6539444051635428
1202	13	1.6342484817507383
1202	14	1.6145680629776393
1202	15	1.6237397113604288
1202	16	1.496047022815425
1202	17	1.559308006987571
1202	18	1.5934284478951355
1202	19	1.5268062929751878
1202	20	1.8056956490475713
1202	21	1.7675555619897878
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2211	2	-0.13086625445533784
2211	3	0.009676064231552317
2211	4	-0.09329515077393324
2211	5	-0.7685865341845926
2211	6	-0.0019330897249716372
2211	7	0.14614744550671332
2211	8	0.048096847122174324
2211	9	0.5579788683516469
2211	10	-0.052084237627617824
2211	11	0.2361726850634085
2211	12	0.26284708507640886
2211	13	0.09868647830105459
2211	14	0.10965593750403713
2211	15	0.042249918283452814
2211	16	0.05771118913813922
2211	17	0.04461199855807507
2211	18	-0.03520868752629269
2211	19	-0.43154396333409295
2211	20	0.10067719373562056
2211	21	0.4146221958128393
2211	22	0.2855763734454797
2211	23	0.5697107299018356
2211	24	0.6548961014436969
2211	25	0.6738346216255167
2212	1	0.0492927440787021
2212	2	0.10771570434417299
2212	3	0.0860369547571338
2212	4	0.27298139066667204
2212	5	-0.03298796004140314
2212	6	0.24064762744633583
2212	7	0.29225657374161074
2212	8	0.1705569640992728
2212	9	0.6687902804467356
2212	10	0.28785129244029406
2212	11	0.44242519515290013
2212	12	0.18786211411912035
2212	13	0.08160426427237155
2212	14	0.19488194759932043
2212	15	0.257770548006647
2212	16	0.3506161456231993
2212	17	0.07083245004916705
2212	18	0.22595236082312908
2212	19	0.5023458854986771
2212	20	0.6627902242305979
2212	21	0.815661933458415
2212	22	0.22813154250394518
2212	23	0.6421785566644687
2212	24	0.7634342605988707
2212	25	0.8918746631273464
2213	1	-0.21006244895148996
2213	2	-0.24343186733225153
2213	3	-0.323052501912489
2213	4	-0.1023000102844378
2213	5	-0.12400297558117046
2213	6	-0.07741807646169718
2213	7	-0.1382581734406365
2213	8	-0.012742959762078954
2213	9	0.5695530399110211
2213	10	-0.03874306331442057
2213	11	0.18579464233638987
2213	12	0.09862712029757859
2213	13	-0.4403096865745155
2213	14	-0.3365222487635009
2213	15	-0.18503896807608555
2213	16	-0.4257842438072572
2213	17	-0.5600242969583391
2213	18	-0.6627430471427189
2213	19	-0.5314501877442055
2213	20	-0.1112915561655754
2213	21	-0.41404973822175606
2213	22	-0.3168512950960647
2213	23	-0.7598995291350512
2213	24	-0.5858963433927933
2213	25	-0.1863576720824085
2214	1	-0.8515603984442599
2214	2	-0.6060719339351941
2214	3	-0.5636291107127107
2214	4	-0.3390623672885411
2214	5	-0.7636502266680978
2214	6	-0.6742649205306464
2214	7	-0.6455659962028086
2214	8	-0.6153170401172936
2214	9	-10.398782194895183
2214	10	-3.6563872926012095
2214	11	-1.6481555904655565
2214	12	-0.7808733576401927
2214	13	-0.6003775229039547
2214	14	-0.5775484274197211
2214	15	-0.8888606201990541
2214	16	-0.809242663220779
2214	17	-0.7344006652479393
2214	18	-1.1245641442411767
2214	19	-1.1504221590062684
2214	20	-0.8230648594864718
2214	21	-1.4425323628710096
2214	22	-1.1096384431951059
2214	23	-1.4911252082692137
2214	24	-1.716571931564605
2214	25	-9.438843873556312
2215	1	-0.65920232742765
2215	2	-0.449516001159747
2215	3	-0.37582448083491116
2215	4	-0.522206346164225
2215	5	-0.6872319295180915
2215	6	-0.6520290489045308
2215	7	-0.6648049550759119
2215	8	-0.6934363011633842
2215	9	0.12538618415124603
2215	10	0.037086803311908056
2215	11	0.09110828477393085
2215	12	0.0778359547276537
2215	13	-0.03868172096424871
2215	14	-0.18876451485532186
2215	15	-0.5848295789728937
2215	16	-0.2700867423575417
2215	17	-0.4787262357930189
2215	18	-0.3609683452795238
2215	19	-0.5007708392329988
2215	20	-0.18374787565728923
2215	21	-0.3830298960514682
2215	22	-0.25668917927023216
2215	23	0.17437952186966044
2215	24	0.27997625827627104
2215	25	0.2608471535737422
2216	1	-0.7503301525602168
2216	2	-0.8206697508679994
2216	3	-0.747348581624415
2216	4	-0.6085027292615592
2216	5	-1.4563628775589912
2216	6	-2.0490919430053403
2216	7	-1.2559033643022843
2216	8	-2.188677675551677
2216	9	-0.9200035235607373
2216	10	-1.3873432514498987
2216	11	-0.9795359428608208
2216	12	-2.057029706204357
2216	13	-1.8990993882539549
2216	14	-1.9798091074402002
2216	15	-1.6439789384777015
2216	16	-2.6375333645435006
2216	17	-2.978773735248101
2216	18	-2.5011168136270427
2216	19	-3.945585393644709
2216	20	-2.719459991345712
2216	21	-2.964716420865958
2216	22	-2.822061449618804
2216	23	-2.3069896004947203
2216	24	-2.307644805055695
2216	25	-2.3499623239418987
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66468.0
3	4.0
4	922.0
5	9390.0
6	13094.0
7	10142.0
8	7776.0
9	6332.0
10	5526.0
11	4898.0
12	4310.0
13	4096.0
14	4098.0
15	4181.0
16	4063.0
17	4484.0
18	5256.0
19	6469.0
20	8809.0
21	11530.0
22	13977.0
23	16402.0
24	18773.0
25	21352.0
26	23393.0
27	25500.0
28	28405.0
29	32746.0
30	39804.0
31	49222.0
32	60893.0
33	74325.0
34	93111.0
35	116704.0
36	153899.0
37	233920.0
38	503962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.140691024829785	18.815898927131695	12.888343014506354	23.15506703353217
2	15.363295605054342	21.9962895655285	39.67694815580034	22.96346667361682
3	17.911971918584747	29.501656760851873	29.392015561823776	23.194355758739608
4	11.471412839949933	17.575302712491105	36.752178268201995	34.20110617935697
5	11.61198462282307	39.593504991420254	34.64646476616642	14.148045619590258
6	28.627909800982792	38.38309997660015	18.81359797872687	14.175392243690185
7	25.721877453085888	33.17481523171942	23.56485033398996	17.538456981204735
8	25.84085393599272	34.44817182997118	21.265985230547553	18.444989003488548
9	26.28927888549375	15.249329415591687	21.05175839171136	37.40963330720321
10	15.065058036425864	30.238254800915122	32.65096314572894	22.04572401693008
11	32.24317977519435	24.209124130195054	24.442474509522746	19.105221585087858
12	22.71807737077966	27.93730247822262	30.722496527171	18.622123623826717
13	27.847675371878893	22.428657065817468	28.01560730346929	21.708060258834344
14	21.718175765172536	21.514694487819558	30.310713244824406	26.4564165021835
15	22.716628883219233	29.048313250942314	26.566690104843882	21.66836776099457
16	21.295517970827504	28.319107255092568	28.10134725670385	22.284027517376074
17	20.441316378430958	28.043417219264892	29.612887021158873	21.902379381145273
18	19.88106620979409	25.980895711961743	33.01757466537283	21.12046341287134
19	21.51132101516203	25.587111596375994	32.74696720966702	20.154600178794965
20	21.28579079196495	25.12494046222639	33.220596633657266	20.368672112151394
21	22.235590593721565	24.49385805277525	32.90088527449196	20.36966607901122
22	21.552901256398545	24.804585567381622	32.97203507655744	20.670478099662397
23	21.075528700906347	24.911024228422487	33.41069841834015	20.602748652331023
24	20.966672807543922	25.194992598367076	33.01839123627707	20.819943357811937
25	20.796847713613953	24.8644000960629	33.38826312933803	20.95048906098512
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3469.0
1	3469.0
2	4373.0
3	5277.0
4	5277.0
5	5277.0
6	19352.0
7	33427.0
8	33427.0
9	33427.0
10	26664.5
11	19902.0
12	19902.0
13	19902.0
14	16658.5
15	13415.0
16	13415.0
17	13415.0
18	22022.5
19	30630.0
20	30630.0
21	30630.0
22	42613.5
23	54597.0
24	54597.0
25	54597.0
26	77821.5
27	101046.0
28	101046.0
29	101046.0
30	116271.0
31	131496.0
32	131496.0
33	131496.0
34	139545.0
35	147594.0
36	147594.0
37	147594.0
38	152200.0
39	156806.0
40	156806.0
41	156806.0
42	166772.0
43	176738.0
44	176738.0
45	176738.0
46	187875.5
47	199013.0
48	199013.0
49	199013.0
50	192211.0
51	185409.0
52	185409.0
53	185409.0
54	170395.5
55	155382.0
56	155382.0
57	155382.0
58	135052.5
59	114723.0
60	114723.0
61	114723.0
62	96326.0
63	77929.0
64	77929.0
65	77929.0
66	60445.0
67	42961.0
68	42961.0
69	42961.0
70	32275.0
71	21589.0
72	21589.0
73	21589.0
74	15351.5
75	9114.0
76	9114.0
77	9114.0
78	6949.0
79	4784.0
80	4784.0
81	4784.0
82	3288.5
83	1793.0
84	1793.0
85	1793.0
86	1281.5
87	770.0
88	770.0
89	770.0
90	551.0
91	332.0
92	332.0
93	332.0
94	185.0
95	38.0
96	38.0
97	38.0
98	20.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02511497207736359
2	0.0014216021930583166
3	1.1846684942152638E-4
4	0.004916374250993344
5	0.03198604934381212
6	0.01131358411975577
7	0.018421595085047354
8	0.025351905776206645
9	0.03133448167199372
10	0.03488848715463951
11	0.038205558938442255
12	0.007759578637109978
13	0.003080138084959686
14	0.0071080109652915825
15	0.006337976444051661
16	0.008233446034796083
17	0.008885013706614478
18	0.01409755508116164
19	0.015933791247195298
20	0.013742154532897061
21	0.010188149050251267
22	0.00929964767958982
23	0.008055745760663794
24	0.005627175347522503
25	0.10904873489251503
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1688236.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.09390989209513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.06874397971839	30.437742909232107
2	12.486511183391375	10.262391308738465
3	4.9233577693242925	6.069600616174768
4	2.4421549476077367	4.014307814381065
5	1.4038815924604122	2.8845491829869596
6	0.9116236450980671	2.2477307956297965
7	0.642864490519384	1.8492470812361845
8	0.4589419593508305	1.5087775618611692
9	0.33950642976290407	1.2556481969217332
>10	2.0029594335813736	15.573405804932097
>50	0.1991562523768416	5.770832499761847
>100	0.11295081802163755	8.745990443195959
>500	0.004754263920858619	1.371506478032361
>1k	0.001728823243948589	1.4867755410008927
>5k	1.4406860366238241E-4	0.45675440205558426
>10k+	7.20343018311912E-4	6.064739363858975
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACTTTTTTTTTTTTTTTTTTTTT	29200	1.729616001554285	No Hit
GTATCAACGCAGAGTACTTTTTTTT	25092	1.4862850928424698	No Hit
TATCAACGCAGAGTACTTTTTTTTT	18851	1.116609289222597	No Hit
GGTATCAACGCAGAGTACTTTTTTT	17014	1.0077974880289249	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	12282	0.7275049222975936	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	7715	0.45698587164353804	No Hit
GTGGTATCAACGCAGAGTACTTTTT	3467	0.20536228347221597	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	3062	0.1813727464643569	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	3028	0.17935881002419093	No Hit
GTATAACGCAGAGTACTTTTTTTTT	2810	0.16644592343724457	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2521	0.149327463695834	No Hit
GTGGTATCAACGCAGAGTACATGGG	2032	0.12036231901227079	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	1888	0.1118327058539209	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.923342471076319E-5
2	0.0	0.0	0.0	0.0	5.923342471076319E-5
3	0.0	0.0	0.0	0.0	5.923342471076319E-5
4	0.0	0.0	0.0	0.0	5.923342471076319E-5
5	0.0	0.0	0.0	5.923342471076319E-5	5.923342471076319E-5
6	0.0	0.0	0.0	5.923342471076319E-5	5.923342471076319E-5
7	0.0	0.0	0.0	5.923342471076319E-5	5.923342471076319E-5
8	0.0	0.0	0.0	5.923342471076319E-5	5.923342471076319E-5
9	0.0	0.0	0.0	1.1846684942152638E-4	5.923342471076319E-5
10	0.0	0.0	0.0	1.1846684942152638E-4	5.923342471076319E-5
11	0.0	0.0	0.0	1.7770027413228955E-4	5.923342471076319E-5
12	0.0	0.0	0.0	1.7770027413228955E-4	1.7770027413228955E-4
13	0.0	0.0	0.0	2.3693369884305275E-4	2.9616712355381595E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	455	0.0	17.11965	2
TAACGCA	470	0.0	16.778374	4
ATAACGC	480	0.0	16.625284	3
ACCGTAT	35	0.002168766	16.287441	8
GTATAAC	520	0.0	15.163721	1
CGCGCCA	45	6.7618734E-4	14.778031	10
GTACCGT	40	0.0052756383	14.251511	6
GCGAATC	40	0.0052849813	14.24771	15
CGGTGTT	75	9.691666E-7	13.931096	14
TATCACG	125	7.2759576E-12	13.679019	2
CGGTTTG	160	0.0	13.060402	14
CGCCAGT	60	4.0999483E-4	12.663881	12
GGTATCA	3980	0.0	12.650971	1
GTGCACG	115	5.329639E-9	12.390416	11
TGACTAG	100	1.4423676E-7	12.350212	10
ACGGCCC	120	1.00371835E-8	11.873093	15
GAATGCG	185	0.0	11.809963	11
CTAGAAC	90	7.460083E-6	11.61131	3
AAACGCA	115	7.05204E-8	11.567816	5
CTACGCA	125	1.828812E-8	11.40121	4
>>END_MODULE