FastQCFastQC Report
Thu 26 May 2016
SRR1364323_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364323_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550676
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA157091.0130420539171303No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA95220.6140547735310278No Hit
GTATCAACGCAGAGTACTTTTTTTT89980.5802630594656781No Hit
GTACTTTTTTTTTTTTTTTTTTTTT70720.45605916387433615No Hit
GAACAAAAAAAAAAAAAAAAAAAAA68290.4403885789165499No Hit
TATCAACGCAGAGTACTTTTTTTTT54460.35120166946544606No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN39630.25556595962019146No Hit
GGTATCAACGCAGAGTACTTTTTTT35830.23106051812241887No Hit
GAGTACTTTTTTTTTTTTTTTTTTT35660.22996422205541325No Hit
AAACAAAAAAAAAAAAAAAAAAAAA30650.19765573208071838No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25660.16547621811390645No Hit
GAAAACAAAAAAAAAAAAAAAAAAA19130.12336555154010252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1900.018.4873312
TATCACG1150.016.5104262
ATAACGC2400.015.0313673
TAACGCA2650.014.3455894
CGAAGGG400.005248709614.26239914
CTACCGT400.005281530314.2490046
GTATAAC3150.014.1644391
GTACAAA6200.013.6273171
TCACGCA1400.013.5770754
ATCACGC1400.013.5621363
GAACAAA17450.013.4917521
ACGAAAC500.001490108213.3111417
CGCATAT957.39019E-812.9995128
GCCCCCG1251.0186341E-1012.93040318
AGCAGTT3250.012.5687597
TTGACCG2050.012.52226918
GTATCAC1850.012.3154871
CGCCAGT853.995199E-612.27973812
TACGATA550.003084212512.0826292
CGTTTTG657.9693756E-411.70172118
Produced by FastQC (version 0.11.5)