FastQCFastQC Report
Thu 26 May 2016
SRR1364323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364323_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550676
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT335702.1648622923163834No Hit
GTATCAACGCAGAGTACTTTTTTTT270141.7420789384758648No Hit
TATCAACGCAGAGTACTTTTTTTTT198631.2809252222901497No Hit
GGTATCAACGCAGAGTACTTTTTTT165821.0693400813580658No Hit
GAGTACTTTTTTTTTTTTTTTTTTT162411.047349672014012No Hit
ACGCAGAGTACTTTTTTTTTTTTTT107800.6951806824894433No Hit
GCAGAGTACTTTTTTTTTTTTTTTT42970.2771049529366547No Hit
GTATAACGCAGAGTACTTTTTTTTT35490.22886792598840766No Hit
GTGGTATCAACGCAGAGTACTTTTT32050.2066840526325293No Hit
ATCAACGCAGAGTACTTTTTTTTTT32020.2064905886207048No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA27340.1763102027760796No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT20500.13220040808008895No Hit
CCTATAAGCAGTTCTTGTATTTTTA18090.1166587991301858No Hit
CCCATGTACTCTGCGTTGATACCAC17590.11343439893311046No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT16260.10485749440889006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2000.017.5735682
TATAACG4050.016.4184172
ATAACGC4150.016.2516863
TCACGCA2250.015.6219564
TAACGCA4400.015.5451914
ATCACGC2350.015.3604493
GTATAAC5050.015.2408081
GTCGGTT508.736785E-515.19533112
CTAGCAC456.766493E-414.7765733
CTGGTCG759.670985E-713.9335469
TGCGGCT707.2613675E-613.57163610
CCGTCTA500.001499371313.3002039
CTTCGCG500.0015008313.29848815
TATAAGC3450.013.2163133
GTACTAG655.441793E-513.1565941
AAGACCG655.4434408E-513.1561695
TATGACG655.4533382E-513.1536234
GTACTAA801.9947074E-613.0652281
AGCAGTT3500.013.029197
GCCCCCG1207.421477E-1012.66522618
Produced by FastQC (version 0.11.5)