##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364323_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1550676 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.431785234310713 31.0 30.0 34.0 26.0 34.0 2 29.403341510412233 31.0 30.0 34.0 25.0 34.0 3 29.470016302567398 31.0 30.0 34.0 25.0 34.0 4 32.78089297828818 35.0 33.0 37.0 26.0 37.0 5 32.40693994103217 35.0 32.0 37.0 25.0 37.0 6 32.236710957027775 35.0 32.0 37.0 22.0 37.0 7 31.95496351268737 35.0 32.0 37.0 19.0 37.0 8 31.83290642274724 35.0 32.0 37.0 17.0 37.0 9 32.52023827027696 37.0 32.0 39.0 11.0 39.0 10 32.683219447518375 37.0 32.0 39.0 17.0 39.0 11 32.60379344234386 37.0 32.0 39.0 13.0 39.0 12 32.496199721927724 37.0 32.0 39.0 12.0 39.0 13 32.55656113849702 37.0 32.0 39.0 13.0 39.0 14 33.451510180076305 38.0 32.0 41.0 11.0 41.0 15 33.48479824283087 38.0 32.0 41.0 11.0 41.0 16 33.45455788314258 38.0 32.0 41.0 11.0 41.0 17 33.468581444479696 38.0 32.0 41.0 11.0 41.0 18 33.43420224469844 38.0 32.0 41.0 10.0 41.0 19 33.34959204888707 39.0 32.0 41.0 10.0 41.0 20 33.17432784153492 38.0 32.0 41.0 8.0 41.0 21 33.077875068679724 38.0 32.0 41.0 2.0 41.0 22 33.017905094294356 38.0 32.0 41.0 2.0 41.0 23 32.6765036667879 38.0 32.0 41.0 2.0 41.0 24 32.576333805385524 38.0 32.0 41.0 2.0 41.0 25 32.42164836497115 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3630520993533004 1101 2 0.5884333127382355 1101 3 -1.1191279326780545 1101 4 -0.3954505006459499 1101 5 0.2878903738233376 1101 6 0.7303779062693607 1101 7 0.7563200962968395 1101 8 0.8487804262115546 1101 9 1.5707871604322392 1101 10 1.1054508911651695 1101 11 1.2606681111042448 1101 12 1.4322258832953167 1101 13 1.2363421137541053 1101 14 1.2017093935429202 1101 15 1.2017430319620885 1101 16 1.0299227002523779 1101 17 1.0149355310686872 1101 18 1.1704751376163358 1101 19 1.304951499731125 1101 20 1.4264462619646139 1101 21 1.3422440215889324 1101 22 1.4791496205237067 1101 23 1.7422146982786444 1101 24 1.7693651311618552 1101 25 1.80884638947213 1102 1 0.7651414704643713 1102 2 0.8046033815614031 1102 3 0.9184964230423063 1102 4 0.6893041480678903 1102 5 0.8528154228755938 1102 6 0.9823349337926288 1102 7 1.1435199699910328 1102 8 1.2782786831313757 1102 9 1.8575763527350873 1102 10 1.55945296276046 1102 11 1.566964950758873 1102 12 1.6537731369689865 1102 13 1.5616206390662697 1102 14 1.499833857268257 1102 15 1.3871946877382584 1102 16 1.5510252959717832 1102 17 1.415697341111951 1102 18 1.4575917199791277 1102 19 1.5878590759796367 1102 20 1.617398858284048 1102 21 1.7619959551778805 1102 22 1.802025785327288 1102 23 1.97127598509784 1102 24 2.168391173859824 1102 25 2.069100486160579 1103 1 -1.9933181434188008 1103 2 -1.9391018139812175 1103 3 -1.8901353109345536 1103 4 -1.5547071373320662 1103 5 -3.8418067114135717 1103 6 -7.801802957470059 1103 7 -8.90094956297795 1103 8 -10.164815219913553 1103 9 -10.590290777650324 1103 10 -7.860633186008084 1103 11 -11.07696825954704 1103 12 -10.669921323248207 1103 13 -11.387260136716257 1103 14 -11.745419531900602 1103 15 -11.383552881287223 1103 16 -5.714523181563887 1103 17 -4.117710814186513 1103 18 -3.629120431344603 1103 19 -3.379688745015468 1103 20 -8.377484117796584 1103 21 -5.439814854673781 1103 22 -11.728292636846039 1103 23 -12.675389853499645 1103 24 -13.623137234827809 1103 25 -13.800837940981165 1104 1 0.2833899627182568 1104 2 0.4509514411089661 1104 3 0.4954489262371595 1104 4 0.6062856291341916 1104 5 0.6560160203690373 1104 6 0.5822294195895452 1104 7 0.4438283853442613 1104 8 0.5479634576981098 1104 9 0.9640434065207018 1104 10 0.905919988521056 1104 11 0.8728104095845097 1104 12 0.6282626612162332 1104 13 0.5629637381851609 1104 14 0.6130241135290433 1104 15 0.6139857668136557 1104 16 0.3093949684153401 1104 17 0.298764343331527 1104 18 0.35867881053357564 1104 19 0.5113206424661954 1104 20 0.7538982249185793 1104 21 0.8091018112862116 1104 22 0.5016879437022865 1104 23 0.9222013712826254 1104 24 0.8504578229641382 1104 25 1.1033876977831127 1105 1 0.6576631856749167 1105 2 0.6826136016826112 1105 3 0.20403506796659698 1105 4 0.676750223902296 1105 5 0.5903348166544475 1105 6 0.7624045694661206 1105 7 0.9845708508251789 1105 8 0.8325390560741042 1105 9 1.0257324228059375 1105 10 0.7550464997199811 1105 11 1.1485403514423496 1105 12 1.2196783279522236 1105 13 1.14195072627642 1105 14 0.9585698631831789 1105 15 0.8267585060058167 1105 16 0.9555551441981791 1105 17 1.06587468684954 1105 18 1.1759944148775077 1105 19 1.2510824675695034 1105 20 1.392187054931874 1105 21 1.284981403658108 1105 22 1.000128142271791 1105 23 1.4803819646401308 1105 24 1.5911080567083005 1105 25 1.533852202127953 1106 1 -0.034833188827612105 1106 2 0.25569068835681463 1106 3 -0.010383341889667719 1106 4 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0.11106101782737454 1107 16 -0.10395185398377293 1107 17 -0.3257880257423835 1107 18 -0.16528425157513027 1107 19 0.20027944271999587 1107 20 0.022268453479270534 1107 21 0.10032334332093029 1107 22 0.0534980746624143 1107 23 0.33721004477007455 1107 24 0.4244690862432918 1107 25 0.024692478957966557 1108 1 0.051581015544577724 1108 2 0.07359051227254909 1108 3 0.1842154851327109 1108 4 -0.052664870525589436 1108 5 0.19086297660878415 1108 6 0.18012594268919457 1108 7 -0.18967695216688085 1108 8 0.24833250332090628 1108 9 0.37300326051001065 1108 10 0.3212206921052214 1108 11 0.34391419275253554 1108 12 0.5144332897301709 1108 13 0.10556231549557538 1108 14 0.10449469728078498 1108 15 -0.10452132376812528 1108 16 -0.5982916573879251 1108 17 -0.6700836605931286 1108 18 -0.5823435276053459 1108 19 -0.4462675203573454 1108 20 0.06841226403491873 1108 21 -0.15798203538039246 1108 22 0.17898713396380117 1108 23 -0.3042610568795894 1108 24 0.035128571352075255 1108 25 -0.17213125963181852 1109 1 0.27189480297865387 1109 2 0.32154761814059896 1109 3 0.22662031662522963 1109 4 0.42885217145552446 1109 5 0.4003520917015777 1109 6 0.45922879174682407 1109 7 0.6481876888591742 1109 8 0.4463186565494439 1109 9 1.189403748878469 1109 10 0.6759752600313433 1109 11 0.7855517804167107 1109 12 0.878786085888116 1109 13 0.9018391791262417 1109 14 0.5791000898378229 1109 15 0.21986917355098967 1109 16 0.6231451910960075 1109 17 0.5961839831326188 1109 18 0.6008971636907674 1109 19 0.7679521895280388 1109 20 0.9911540518127993 1109 21 0.8400856161465526 1109 22 0.684301437450209 1109 23 1.06353720842516 1109 24 0.6439524449341008 1109 25 0.7321448294025714 1110 1 -0.09561519335995072 1110 2 -0.16212802229901868 1110 3 -0.03895767491895441 1110 4 -0.025005494128762962 1110 5 0.032944588111860185 1110 6 0.04730549059708977 1110 7 -0.16935758673591295 1110 8 -0.15283780505812317 1110 9 -3.2870898192895908 1110 10 -1.508596878880276 1110 11 -0.44252968385204383 1110 12 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2215 1 -0.5655316537208712 2215 2 -0.48821952998519436 2215 3 -0.41503770599408796 2215 4 -0.48000803537834713 2215 5 -0.6446528148950144 2215 6 -0.630565900733906 2215 7 -0.8459876753832773 2215 8 -0.6755659090084762 2215 9 0.10202530877551652 2215 10 -0.03326055742418532 2215 11 -0.0049608714794970865 2215 12 -0.07020868189280804 2215 13 -0.14394496369482823 2215 14 -0.510822116915918 2215 15 -0.7705332309675299 2215 16 -0.6195998509519072 2215 17 -0.7580719726743013 2215 18 -0.6776622175756373 2215 19 -0.4862498986533268 2215 20 -0.31415407164136866 2215 21 -0.4597319207864743 2215 22 -0.4404550611668441 2215 23 -0.1975500145174891 2215 24 0.11848854419299215 2215 25 0.09190170642856543 2216 1 -0.9595272447990979 2216 2 -1.010798657655073 2216 3 -0.9606575131781554 2216 4 -0.9064267135488073 2216 5 -1.8504036421401366 2216 6 -2.3387452403799784 2216 7 -1.6683534533599556 2216 8 -2.4216454367090243 2216 9 -1.1752279970573767 2216 10 -1.7808145901797694 2216 11 -1.4109104550241014 2216 12 -2.4804201086917743 2216 13 -2.164405187826464 2216 14 -2.462699685782429 2216 15 -2.088409966579878 2216 16 -3.1911976158162787 2216 17 -3.4585583046734385 2216 18 -2.7863209072834607 2216 19 -4.250037221973109 2216 20 -2.8894225042422086 2216 21 -3.2918632544624202 2216 22 -3.2263158939860723 2216 23 -2.890161064520747 2216 24 -2.8394936415654612 2216 25 -2.912499203283538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61483.0 3 1.0 4 833.0 5 8744.0 6 12430.0 7 9833.0 8 7376.0 9 5928.0 10 5090.0 11 4614.0 12 4223.0 13 3871.0 14 3745.0 15 3817.0 16 3942.0 17 4192.0 18 4828.0 19 6215.0 20 8648.0 21 11370.0 22 13435.0 23 15437.0 24 17500.0 25 19672.0 26 21603.0 27 23683.0 28 25996.0 29 30323.0 30 36431.0 31 45046.0 32 55307.0 33 68434.0 34 85592.0 35 107062.0 36 141857.0 37 218227.0 38 453888.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.15385374582559 18.31147728159699 11.616874777047629 22.91779419552979 2 15.611066327024151 22.557379163576567 38.30832231644794 23.523232192951344 3 18.35129862801683 30.107662792009933 29.255066342076834 22.285972237896402 4 11.34895023177706 17.25539587831511 36.10408170109143 35.2915721888164 5 11.507956219023578 40.19324319704084 34.11230115903342 14.186499424902156 6 28.846131520935746 37.331103523623895 19.28318884286807 14.539576112572284 7 25.434879780388048 33.703158792314866 23.807269356818463 17.054692070478612 8 27.04040230722886 32.92916404882284 21.886227969795996 18.144205674152303 9 26.792275854238813 14.473612910217836 21.471700839659594 37.26241039588376 10 14.815765757338632 31.14535077629524 32.86367046658999 21.175212999776146 11 32.77934578203271 23.216448024153358 24.785706682493654 19.218499511320275 12 22.579153507467932 27.74424515482441 31.46315241625139 18.213448921456273 13 27.934849832294294 21.900991401576 28.818875904227497 21.345282861902206 14 21.218849820681505 20.891813648068837 31.718187815887212 26.171148715362445 15 22.638727747742283 28.335009057941807 27.88640070477172 21.139862489544186 16 20.73232943731906 28.257228522100743 29.096732210770437 21.913709829809765 17 20.61026756605632 27.125962491607723 30.853454908731386 21.410315033604572 18 19.696398994876347 24.64068635291811 34.76493367112643 20.897980981079115 19 21.002556119497136 24.891173035035543 34.54573248013251 19.560538365334814 20 20.962069207941802 24.33655459230961 34.61783803063539 20.083538169113194 21 21.552731055717548 23.506060377392565 34.63828321662761 20.302925350262274 22 21.117347837107296 24.06273359505654 34.233950104902114 20.585968462934048 23 20.42166227032047 24.080475708306192 35.06433284102855 20.433529180344788 24 20.60006397655569 24.413053797060176 34.55694945336161 20.42993277302253 25 20.375199081208347 24.207629353559835 34.839711786575904 20.577459778655914 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3913.0 1 3913.0 2 5006.5 3 6100.0 4 6100.0 5 6100.0 6 22484.0 7 38868.0 8 38868.0 9 38868.0 10 31852.5 11 24837.0 12 24837.0 13 24837.0 14 19059.0 15 13281.0 16 13281.0 17 13281.0 18 20257.5 19 27234.0 20 27234.0 21 27234.0 22 39545.0 23 51856.0 24 51856.0 25 51856.0 26 72409.5 27 92963.0 28 92963.0 29 92963.0 30 103572.0 31 114181.0 32 114181.0 33 114181.0 34 121147.5 35 128114.0 36 128114.0 37 128114.0 38 132069.5 39 136025.0 40 136025.0 41 136025.0 42 147945.5 43 159866.0 44 159866.0 45 159866.0 46 171446.5 47 183027.0 48 183027.0 49 183027.0 50 177853.0 51 172679.0 52 172679.0 53 172679.0 54 158016.0 55 143353.0 56 143353.0 57 143353.0 58 125559.0 59 107765.0 60 107765.0 61 107765.0 62 89079.5 63 70394.0 64 70394.0 65 70394.0 66 54481.0 67 38568.0 68 38568.0 69 38568.0 70 29342.5 71 20117.0 72 20117.0 73 20117.0 74 14406.0 75 8695.0 76 8695.0 77 8695.0 78 6964.5 79 5234.0 80 5234.0 81 5234.0 82 3701.0 83 2168.0 84 2168.0 85 2168.0 86 1570.5 87 973.0 88 973.0 89 973.0 90 707.0 91 441.0 92 441.0 93 441.0 94 232.5 95 24.0 96 24.0 97 24.0 98 12.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02947101780126861 2 0.0016766881024791768 3 6.44880039415068E-5 4 0.003095424189192326 5 0.03256644199046093 6 0.010962960670056157 7 0.019668841202159577 8 0.026955985647549842 9 0.033791714065349567 10 0.03591981819541929 11 0.03746753029001545 12 0.0072871444453902695 13 0.0027729841694847926 14 0.005352504327145065 15 0.006642264405975201 16 0.007545096461156295 17 0.009221784563635472 18 0.014187360867131496 19 0.017992153099680396 20 0.01592853697355218 21 0.012575160768593826 22 0.011801304721295744 23 0.009415248575459993 24 0.006964704425682735 25 0.10866228664143897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1550676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.27316628280857 #Duplication Level Percentage of deduplicated Percentage of total 1 72.17308033833866 26.1794614425506 2 12.387951891649235 8.987004777384513 3 5.242741270312493 5.7051247762736415 4 2.7314949315571284 3.963198794120821 5 1.6974429187211064 3.0785814623173304 6 1.108876787436819 2.413348325870538 7 0.787609547616325 1.999836446063021 8 0.5749861369332601 1.6685254204231903 9 0.4165075779307388 1.3597243769098382 >10 2.498991996839283 16.800986832175855 >50 0.22053535446135852 5.550271511515489 >100 0.1467342437073633 9.868173867042263 >500 0.008936304449452948 2.1532507146923874 >1k 0.0030383435128140024 2.230397952317604 >5k 0.0 0.0 >10k+ 0.001072356533934354 8.04211330034295 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTT 33570 2.1648622923163834 No Hit GTATCAACGCAGAGTACTTTTTTTT 27014 1.7420789384758648 No Hit TATCAACGCAGAGTACTTTTTTTTT 19863 1.2809252222901497 No Hit GGTATCAACGCAGAGTACTTTTTTT 16582 1.0693400813580658 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 16241 1.047349672014012 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 10780 0.6951806824894433 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 4297 0.2771049529366547 No Hit GTATAACGCAGAGTACTTTTTTTTT 3549 0.22886792598840766 No Hit GTGGTATCAACGCAGAGTACTTTTT 3205 0.2066840526325293 No Hit ATCAACGCAGAGTACTTTTTTTTTT 3202 0.2064905886207048 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 2734 0.1763102027760796 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 2050 0.13220040808008895 No Hit CCTATAAGCAGTTCTTGTATTTTTA 1809 0.1166587991301858 No Hit CCCATGTACTCTGCGTTGATACCAC 1759 0.11343439893311046 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 1626 0.10485749440889006 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.289760078830136E-4 0.0 0.0 0.0 0.0 6 1.934640118245204E-4 0.0 0.0 0.0 0.0 7 1.934640118245204E-4 0.0 0.0 0.0 0.0 8 1.934640118245204E-4 0.0 0.0 0.0 0.0 9 1.934640118245204E-4 0.0 0.0 0.0 0.0 10 1.934640118245204E-4 0.0 0.0 0.0 0.0 11 1.934640118245204E-4 0.0 0.0 0.0 0.0 12 1.934640118245204E-4 0.0 0.0 0.0 0.0 13 1.934640118245204E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 200 0.0 17.573568 2 TATAACG 405 0.0 16.418417 2 ATAACGC 415 0.0 16.251686 3 TCACGCA 225 0.0 15.621956 4 TAACGCA 440 0.0 15.545191 4 ATCACGC 235 0.0 15.360449 3 GTATAAC 505 0.0 15.240808 1 GTCGGTT 50 8.736785E-5 15.195331 12 CTAGCAC 45 6.766493E-4 14.776573 3 CTGGTCG 75 9.670985E-7 13.933546 9 TGCGGCT 70 7.2613675E-6 13.571636 10 CCGTCTA 50 0.0014993713 13.300203 9 CTTCGCG 50 0.00150083 13.298488 15 TATAAGC 345 0.0 13.216313 3 GTACTAG 65 5.441793E-5 13.156594 1 AAGACCG 65 5.4434408E-5 13.156169 5 TATGACG 65 5.4533382E-5 13.153623 4 GTACTAA 80 1.9947074E-6 13.065228 1 AGCAGTT 350 0.0 13.02919 7 GCCCCCG 120 7.421477E-10 12.665226 18 >>END_MODULE