FastQCFastQC Report
Thu 26 May 2016
SRR1364322_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364322_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317068
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA83470.6337561917835678No Hit
GTATCAACGCAGAGTACTTTTTTTT72510.5505410502722715No Hit
TATCAACGCAGAGTACTTTTTTTTT41250.31319567402746096No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39600.30066784706636257No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA38080.2891270610173507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN34590.2626288088390273No Hit
GGTATCAACGCAGAGTACTTTTTTT29880.22686755733189173No Hit
GAACAAAAAAAAAAAAAAAAAAAAA23770.1804766344638242No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23120.17554142990339147No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21830.1657469470065327No Hit
AAACAAAAAAAAAAAAAAAAAAAAA14640.11115599194574616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCGA504.5214347E-617.11572819
TAACGCA1400.016.2895074
AATGCGT551.1402844E-515.52775812
TATCACG508.75458E-515.1913732
TATAACG1250.015.1913732
ATAACGC1450.014.4056123
CGCATAT801.2920282E-714.2462558
ATGCGTA602.5944564E-514.23431913
GAATGCG602.5953883E-514.23377611
GGTATCA14750.013.002291
GTATAAC2200.012.5151431
TTATACC1301.9645086E-1012.4259694
ATTATAC1800.012.1323
GTATAAA2500.011.7728651
ATCGTAG658.0963963E-411.679440513
TAGACTA907.438657E-611.6138155
CTTACAC1900.011.4934743
ACGCCCC752.0549838E-411.41092217
AGGGACG752.0688662E-411.4026545
GTTATCA2250.011.3930961
Produced by FastQC (version 0.11.5)