##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364322_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1317068
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.38855472914079	30.0	25.0	31.0	2.0	34.0
2	25.289594766557233	30.0	25.0	31.0	2.0	34.0
3	24.8170755040742	30.0	22.0	31.0	2.0	34.0
4	28.40087907382155	35.0	27.0	37.0	2.0	37.0
5	29.020563099247724	35.0	28.0	37.0	2.0	37.0
6	29.15032025681286	35.0	29.0	37.0	2.0	37.0
7	28.779050132567185	35.0	28.0	37.0	2.0	37.0
8	28.235577813749934	35.0	26.0	37.0	2.0	37.0
9	29.15028912706102	35.0	27.0	39.0	2.0	39.0
10	28.55824680274671	35.0	25.0	39.0	2.0	39.0
11	29.121435643414006	35.0	27.0	39.0	2.0	39.0
12	28.67462500037963	35.0	25.0	39.0	2.0	39.0
13	28.309013657609174	35.0	23.0	39.0	2.0	39.0
14	28.569193845723987	35.0	19.0	40.0	2.0	41.0
15	29.22411067613821	36.0	25.0	40.0	2.0	41.0
16	29.583295623308743	36.0	27.0	40.0	2.0	41.0
17	29.74507390658645	36.0	27.0	40.0	2.0	41.0
18	29.559838216401886	36.0	27.0	40.0	2.0	41.0
19	29.5164296756128	36.0	26.0	40.0	2.0	41.0
20	29.349932577513083	36.0	26.0	40.0	2.0	41.0
21	29.285520565377034	36.0	25.0	40.0	2.0	41.0
22	29.33543066872781	36.0	26.0	40.0	2.0	41.0
23	29.3257318528732	36.0	26.0	40.0	2.0	41.0
24	29.149803958489613	36.0	25.0	40.0	2.0	41.0
25	28.974059805568125	36.0	25.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9286968488903931
1101	2	0.9376220932071675
1101	3	1.6566492501523307
1101	4	1.2353274200808215
1101	5	0.6983083869961888
1101	6	0.6015841719921902
1101	7	0.7615559238595182
1101	8	0.9830615117558885
1101	9	1.0246697772444904
1101	10	1.3522909475947067
1101	11	0.8515313123766148
1101	12	1.1173032601280006
1101	13	1.1326234374934998
1101	14	1.4959260150330493
1101	15	0.7549953286295299
1101	16	0.20181794731846026
1101	17	-0.43614625461791334
1101	18	-6.309027550961634
1101	19	-7.566479953933548
1101	20	-8.44158990224225
1101	21	-10.201372150417427
1101	22	-10.373425230816093
1101	23	-11.69469266443944
1101	24	-11.436736518863452
1101	25	-11.659409652453128
1102	1	1.646555080553867
1102	2	1.7595790438591763
1102	3	2.4864219813470037
1102	4	1.8570537081387108
1102	5	1.3198175300932817
1102	6	1.356244879395824
1102	7	1.456672894529131
1102	8	1.7205001790898073
1102	9	1.9343791768890064
1102	10	-1.2403336015778414
1102	11	0.7135062098439278
1102	12	1.566074305932645
1102	13	2.0313375366726625
1102	14	2.582845835334812
1102	15	2.0289934419130518
1102	16	1.7275575344266656
1102	17	1.5823772171704995
1102	18	1.661912413489869
1102	19	1.2513411761405955
1102	20	1.5801116514227544
1102	21	1.8901278467064806
1102	22	1.7352320943031216
1102	23	1.7589015879779168
1102	24	1.8687605561507858
1102	25	1.9077298437705608
1103	1	0.7018777597958561
1103	2	1.2289485312401922
1103	3	-1.5826222069334968
1103	4	0.7397277104092552
1103	5	-2.58251632721176
1103	6	-6.484675127590464
1103	7	-6.34529854901292
1103	8	-0.9512970438601549
1103	9	-5.52729447606707
1103	10	-2.5510160747242914
1103	11	-7.471663207674194
1103	12	-0.672027169920991
1103	13	0.8224256512478085
1103	14	1.6193382814093873
1103	15	1.2539519608196947
1103	16	0.6968506092672904
1103	17	0.9420292126294108
1103	18	1.3278804512158295
1103	19	-4.357284708061659
1103	20	-0.08843258052205627
1103	21	-5.664037728352465
1103	22	-1.9937981213985623
1103	23	-0.7037654280032228
1103	24	-5.853992915161761
1103	25	-2.9005183441606235
1104	1	1.378138938922362
1104	2	1.4385300194749746
1104	3	2.352549712837277
1104	4	1.7231869445848247
1104	5	1.4426964017975337
1104	6	1.0914840284397513
1104	7	1.1862309291497972
1104	8	1.4806079661250848
1104	9	1.5789723468902999
1104	10	1.8686136031432063
1104	11	1.5651627886790251
1104	12	1.7065002243657155
1104	13	2.037694384424853
1104	14	2.4200133704082596
1104	15	1.6088620936667901
1104	16	0.7188941101789865
1104	17	0.7932817089392117
1104	18	0.6379438357777829
1104	19	1.1438114975474427
1104	20	-8.93774229659395
1104	21	-3.5633387616076533
1104	22	-1.2575495323562755
1104	23	-0.250869708631857
1104	24	0.45444244126786515
1104	25	0.8988434688115063
1105	1	1.267122039325848
1105	2	1.2617915385729965
1105	3	2.1864497075465827
1105	4	1.494388016089971
1105	5	1.3102855987534312
1105	6	1.1277795945581346
1105	7	1.4987579047374133
1105	8	1.5784538175233607
1105	9	1.6945559524160139
1105	10	1.9922850119441406
1105	11	1.7146054556352865
1105	12	1.9547508139998477
1105	13	1.9596064976123309
1105	14	2.71935815949049
1105	15	1.938862288587167
1105	16	1.6373157563650906
1105	17	0.7838990774584573
1105	18	1.401164310213975
1105	19	1.400191659108586
1105	20	1.2257736214392487
1105	21	1.1856177182417582
1105	22	1.2137400886906669
1105	23	1.2506033324879873
1105	24	1.3630094702079703
1105	25	1.294618151996282
1106	1	0.7433621221358315
1106	2	1.1293293447226098
1106	3	1.5519791542721428
1106	4	1.2419963864747245
1106	5	0.483943727884661
1106	6	0.2187562449585556
1106	7	0.8245078032800635
1106	8	0.8340294834449828
1106	9	0.8844904858515825
1106	10	-2.2126389883525555
1106	11	-0.3093921008843701
1106	12	0.3784508725356588
1106	13	1.296133316131943
1106	14	0.4540692812473388
1106	15	1.0517156944123052
1106	16	0.6383714324726064
1106	17	-5.8381320254195295
1106	18	-2.0359179607109255
1106	19	-0.5566243612783168
1106	20	0.33443243325205074
1106	21	0.5416420642257229
1106	22	0.5328790784069781
1106	23	0.25815322375500216
1106	24	0.5547333852757674
1106	25	0.5722980551263639
1107	1	0.3959524481096217
1107	2	0.8723860266940093
1107	3	1.0408175434265594
1107	4	0.4906624054150015
1107	5	0.11951781643304216
1107	6	0.3051803401934734
1107	7	0.5411541925166361
1107	8	0.5328567843887306
1107	9	0.5229844776564363
1107	10	-2.3969532428530123
1107	11	-0.2517534766626852
1107	12	-0.04898202815285657
1107	13	0.75274541150036
1107	14	1.0609234804957879
1107	15	0.9818356949444293
1107	16	-5.272088057282993
1107	17	-2.002010924701654
1107	18	-0.4658756406679636
1107	19	0.2558152061064263
1107	20	0.6747513470881827
1107	21	0.7556839725742286
1107	22	0.3823802489588779
1107	23	0.010923467674633258
1107	24	0.2543189601928404
1107	25	0.5967357619508995
1108	1	-0.7123275226000878
1108	2	-3.24110217032802
1108	3	-0.40022921082454843
1108	4	-0.9979948458930679
1108	5	-1.111684467761222
1108	6	-0.6468906682106734
1108	7	-0.23220752201138595
1108	8	-0.0015859248883351995
1108	9	-0.5362851611277932
1108	10	-3.0952915139413655
1108	11	-1.4024791491905226
1108	12	-5.5451995552529105
1108	13	-1.8300475240852876
1108	14	-5.562450422752857
1108	15	-2.622772482052607
1108	16	-7.306097156326388
1108	17	-3.3187448706154896
1108	18	-2.1212853311211575
1108	19	-0.8047407863543121
1108	20	-0.8729509156240489
1108	21	-0.38311566727476176
1108	22	-0.5421267383069477
1108	23	-0.41279148625107
1108	24	-0.1152239020736836
1108	25	-0.435744143005099
1109	1	-8.23876623347972
1109	2	-6.096571965332934
1109	3	-5.097854544534254
1109	4	-4.980885247045805
1109	5	-2.421792804646131
1109	6	-1.457373304236011
1109	7	-1.1268677976874315
1109	8	-0.5731234156865241
1109	9	-5.339328399943099
1109	10	-1.464347468318845
1109	11	-0.9688883035834976
1109	12	-5.497644653433213
1109	13	-7.435850567554727
1109	14	-7.90195035172135
1109	15	-3.8103611561250013
1109	16	-1.9908245060394023
1109	17	-1.5663062759692359
1109	18	-0.9833829653107458
1109	19	-0.6696526980612276
1109	20	-0.360793458146091
1109	21	-0.36983323677955227
1109	22	-0.551036579952811
1109	23	-0.9594661047565296
1109	24	-0.5351321884062976
1109	25	-0.43632807733877854
1110	1	-2.2375590410270867
1110	2	-2.038581793220825
1110	3	-1.4157787368722055
1110	4	-2.1257516946741077
1110	5	-5.443854246717994
1110	6	-3.906051193768512
1110	7	-8.657892308388856
1110	8	-9.407546240208358
1110	9	-11.159195166136076
1110	10	-8.993426387184112
1110	11	-11.715914418509563
1110	12	-6.654261107600476
1110	13	-4.7201893245354825
1110	14	-4.244314155262849
1110	15	-9.22322432823778
1110	16	-10.827467894011608
1110	17	-11.467099200211873
1110	18	-8.427252204906925
1110	19	-11.60322894534253
1110	20	-12.490494215256955
1110	21	-13.167777388032718
1110	22	-14.060913761534005
1110	23	-14.70149377226622
1110	24	-14.948491650749606
1110	25	-15.603456495118577
1111	1	-0.7643025439625362
1111	2	-0.1337303964315275
1111	3	-2.776819708134841
1111	4	-3.236558457371384
1111	5	-1.1341832851137
1111	6	-0.6205127202396845
1111	7	-0.1761836858206891
1111	8	0.09813650353110859
1111	9	-4.279972020371236
1111	10	-4.059055988518651
1111	11	-1.3909455311492103
1111	12	-5.924477186667019
1111	13	-1.2909558815234412
1111	14	0.07835965807852929
1111	15	0.03727291665386545
1111	16	-0.12238875700475305
1111	17	-0.06429241304589084
1111	18	0.3256233778391042
1111	19	0.45493588809694785
1111	20	0.4551070930032033
1111	21	0.5556956457538078
1111	22	0.08265659879714704
1111	23	-0.03492905854215067
1111	24	0.2406403468189282
1111	25	0.3684701957313301
1112	1	-0.12620402896554594
1112	2	0.42487380337905734
1112	3	-2.254224148681068
1112	4	-0.16175802068812573
1112	5	-0.35788408243388403
1112	6	-0.134976686872605
1112	7	0.3263622335701939
1112	8	-2.8906909832540926
1112	9	-0.342041460474249
1112	10	-2.575648319502168
1112	11	-0.5199935989657476
1112	12	0.8367401145744466
1112	13	1.2753971134425726
1112	14	1.8719787639042416
1112	15	1.339697768410165
1112	16	1.135692915828045
1112	17	0.7470233285458576
1112	18	0.8221140493329244
1112	19	0.8770562745802977
1112	20	0.9417821885276645
1112	21	1.0993632719417405
1112	22	1.061343096606734
1112	23	0.7156226273071624
1112	24	1.0179841369123785
1112	25	1.108999160987949
1113	1	-0.893913485995391
1113	2	-0.6400189210605944
1113	3	-3.0792271452051025
1113	4	-1.4094866554717775
1113	5	-1.3439921970209632
1113	6	-1.2556347235500596
1113	7	-0.9910056197199886
1113	8	-8.298098390661501
1113	9	-8.992754633193893
1113	10	-3.2506513160262003
1113	11	-8.84711214145646
1113	12	-7.024047371941194
1113	13	-2.493343147854322
1113	14	-0.8777155906444847
1113	15	-6.638865098664031
1113	16	-2.75087666345998
1113	17	-1.9721963765961803
1113	18	-1.1267772367751903
1113	19	-1.0391309482412296
1113	20	-6.10885906761521
1113	21	-2.331770612752017
1113	22	-1.5570136096140494
1113	23	-1.4468069543780828
1113	24	-7.1377751822773625
1113	25	-4.226893203868816
1114	1	-4.0336916975891235
1114	2	-3.9033000157228486
1114	3	-0.8598916066406694
1114	4	-0.5024525105410973
1114	5	-0.5975070100282096
1114	6	-0.6968906207356795
1114	7	-0.5399888635787846
1114	8	-0.24708434587000383
1114	9	-0.2545616573972076
1114	10	-3.4834387642846103
1114	11	-1.1591216131047801
1114	12	-4.513302293040919
1114	13	-1.4698122475054625
1114	14	-5.897183081561462
1114	15	-2.3850737279863736
1114	16	-1.22674293969229
1114	17	-1.0870925319022895
1114	18	-0.6631129380206033
1114	19	-0.31163605461651755
1114	20	-0.1765687001369436
1114	21	-0.059829467116383483
1114	22	-0.06384892223267258
1114	23	-0.42349567335572047
1114	24	-0.44353770419410665
1114	25	-0.10426518297334653
1115	1	0.8774849344839097
1115	2	1.000311053521834
1115	3	1.5740873518900322
1115	4	0.6428304287693294
1115	5	0.4066352993479576
1115	6	0.07750457383556153
1115	7	-1.9433238386742389
1115	8	0.24533842851061394
1115	9	0.4206361166245536
1115	10	1.0701264163630526
1115	11	0.33263436362705434
1115	12	0.8173333470130544
1115	13	1.0842349204100188
1115	14	1.800933646828284
1115	15	1.0255094474507125
1115	16	0.6849995620302138
1115	17	0.22559093277201114
1115	18	0.7183661909662753
1115	19	0.8768117714392325
1115	20	0.8120241930964838
1115	21	0.8508909703997638
1115	22	0.4847279481201525
1115	23	0.3096314080039093
1115	24	0.15327646986497712
1115	25	0.3078846192974858
1116	1	0.9192038837367633
1116	2	1.0533669791994953
1116	3	1.893823040426149
1116	4	1.3121079890152174
1116	5	0.4562699513605111
1116	6	-0.5353703791612041
1116	7	-4.049606076764025
1116	8	-0.4597571311140136
1116	9	-0.44435909108888083
1116	10	-0.027684510050317357
1116	11	-1.2653890991627286
1116	12	0.3442529183435319
1116	13	-5.525086434591124
1116	14	-0.6314470875666416
1116	15	-0.3806935493121948
1116	16	-0.08618331530360024
1116	17	0.5731395095957375
1116	18	0.7223083320250332
1116	19	1.0026522097546362
1116	20	0.9236027252875694
1116	21	1.2015146829197896
1116	22	0.97329849793541
1116	23	0.9600545393186906
1116	24	1.2526193441742315
1116	25	1.2960512989070736
1201	1	2.0289670116897938
1201	2	2.20087975756595
1201	3	2.8954051368165032
1201	4	2.421294353401681
1201	5	1.9377991320685446
1201	6	1.821329252144384
1201	7	2.101138477502232
1201	8	2.2406566729959074
1201	9	2.6774433316980826
1201	10	2.804645028495404
1201	11	2.322696404068232
1201	12	2.6551952495865834
1201	13	2.744936896512961
1201	14	3.410768205651813
1201	15	2.8121018187742415
1201	16	2.498504846956685
1201	17	2.144651134977895
1201	18	2.161036227730982
1201	19	2.1620541887186455
1201	20	2.4025860037582483
1201	21	2.238069279223293
1201	22	1.163783554737801
1201	23	0.8112973087392028
1201	24	1.2198263319973286
1201	25	1.3604753821651343
1202	1	2.1302879360570337
1202	2	2.3148432955017206
1202	3	2.955701575301461
1202	4	2.6324515100098473
1202	5	2.049668703612241
1202	6	1.8350370370357396
1202	7	1.9918660771918226
1202	8	2.2074465525625264
1202	9	2.4748653745884823
1202	10	-0.9575564064078925
1202	11	1.1501675642282123
1202	12	2.0780379512043012
1202	13	2.585927431225233
1202	14	3.204636102022395
1202	15	2.812300489082265
1202	16	2.4337938236460346
1202	17	2.050683810180857
1202	18	2.253989681049987
1202	19	2.2469633600116268
1202	20	2.296737168544542
1202	21	2.453375660550897
1202	22	2.279222196392528
1202	23	2.130219116583042
1202	24	2.20133833429556
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2211	19	1.0061921474906264
2211	20	1.1380128081167626
2211	21	1.2410391860951115
2211	22	1.0599701256251208
2211	23	0.9060392359430445
2211	24	1.1345330198010082
2211	25	1.0561550533966226
2212	1	1.064806497724593
2212	2	1.2552971825888442
2212	3	1.752084752773463
2212	4	1.2317357024172502
2212	5	0.839561594866165
2212	6	0.749798731883395
2212	7	1.1079565292421591
2212	8	1.3817449141818052
2212	9	1.3087444849718928
2212	10	1.926631277923061
2212	11	1.1215352605242757
2212	12	1.4275399296461089
2212	13	1.471193550015709
2212	14	-4.113470576399468
2212	15	-0.7463810395133059
2212	16	-0.118916956500275
2212	17	0.27852604912624557
2212	18	0.6657509140862921
2212	19	0.9324672066902586
2212	20	1.1898724477829745
2212	21	1.1539775896628548
2212	22	1.0342155316150894
2212	23	1.0235905493527575
2212	24	0.9594436482747994
2212	25	1.0956105269682475
2213	1	0.24191656330771139
2213	2	0.4735118521508177
2213	3	0.8653856235593622
2213	4	0.41800053538866777
2213	5	-0.0827448910630757
2213	6	-0.45983145828571637
2213	7	-0.18647109901780468
2213	8	-5.46865044893811
2213	9	-2.4084255705215156
2213	10	-0.4412757651336854
2213	11	-0.6124253896040379
2213	12	-0.21764223350012912
2213	13	-8.153535014832617
2213	14	-3.081649602961729
2213	15	-1.639101093910007
2213	16	-1.1592675739466998
2213	17	-1.1366299027063533
2213	18	-0.8849526238926941
2213	19	-0.4301756394855225
2213	20	-0.3084331388459667
2213	21	-0.23568813383520393
2213	22	-0.45046346204593846
2213	23	-0.42461743029235066
2213	24	-0.34841316557003665
2213	25	-0.27940044010858855
2214	1	-0.7613851949925241
2214	2	-0.470072138119459
2214	3	-6.997590140969226
2214	4	-2.519722032091334
2214	5	-1.4037988963756831
2214	6	-0.9699626107593033
2214	7	-0.5738223347400933
2214	8	-5.853929645645298
2214	9	-1.6572154216659811
2214	10	-0.004012021364307117
2214	11	-0.4606708979530687
2214	12	-0.052522457195941286
2214	13	-5.8344650607276485
2214	14	-1.463233311755424
2214	15	-0.7131908368315756
2214	16	-0.8571600655060969
2214	17	-0.6733857015143165
2214	18	-0.5682452584692008
2214	19	-0.2441879826767952
2214	20	0.043044813667204807
2214	21	0.07853573879727449
2214	22	-0.03986171195368016
2214	23	-0.19340298543738754
2214	24	0.05791938234375493
2214	25	0.06124889303034209
2215	1	-0.17496960011414942
2215	2	0.02097904519646221
2215	3	-2.9034458728267687
2215	4	-1.0891621159283673
2215	5	-0.6724674626965381
2215	6	-0.978243643374757
2215	7	-0.35625439517758295
2215	8	-0.09196708534225806
2215	9	-0.017020623855863448
2215	10	0.6893990978834559
2215	11	-0.23100547020671058
2215	12	-0.23524871249288282
2215	13	0.3090916148279277
2215	14	0.27287304903928344
2215	15	-0.06787887655638514
2215	16	-0.23212182973995255
2215	17	-0.24104573639681348
2215	18	-0.05285497731542321
2215	19	-0.0830827112958552
2215	20	0.19348403341312093
2215	21	0.5149997461606475
2215	22	0.311427569383973
2215	23	0.1014514741974395
2215	24	-0.2604156107955937
2215	25	-0.1625481550303398
2216	1	0.5575578483526549
2216	2	0.46508846943492443
2216	3	1.5612768290888255
2216	4	0.22662868361160093
2216	5	0.05441808540758686
2216	6	-0.15223543384940186
2216	7	-0.7854246963308462
2216	8	-5.651091795024769
2216	9	-1.7760203189709927
2216	10	-0.45418590083932386
2216	11	-0.7763020619705792
2216	12	0.19013324703394474
2216	13	0.74119092960213
2216	14	1.6316705077827116
2216	15	0.9922020415355313
2216	16	0.4673120732219047
2216	17	0.367326708132385
2216	18	0.5184620664549797
2216	19	0.6575035858362241
2216	20	0.6080528214428007
2216	21	0.45642488868549336
2216	22	0.29684230109117493
2216	23	-0.05324192816465256
2216	24	-0.03439280446576021
2216	25	0.1283663936282231
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	146096.0
3	1.0
4	914.0
5	6614.0
6	10525.0
7	9507.0
8	8059.0
9	6440.0
10	5904.0
11	5482.0
12	5185.0
13	4978.0
14	5011.0
15	4918.0
16	5071.0
17	5216.0
18	6065.0
19	7662.0
20	10760.0
21	13633.0
22	16597.0
23	18955.0
24	21574.0
25	23644.0
26	25879.0
27	28060.0
28	30147.0
29	34168.0
30	38969.0
31	43186.0
32	53125.0
33	66801.0
34	82480.0
35	102246.0
36	127801.0
37	147893.0
38	187502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.40726465583301	18.219377798361087	11.709812906852566	22.66354463895334
2	14.735262528469468	22.780219529101696	38.625848371051404	23.858669571377426
3	16.31557896902033	29.538371291333842	29.349133612923623	24.796916126722206
4	10.526712742032801	17.48822213689747	36.12688095368695	35.85818416738278
5	12.06586283452161	41.07391283810244	32.38934102119087	14.470883306185078
6	30.042076953768653	38.00928647354619	17.699656135499062	14.248980437186098
7	27.685998872964863	34.69501515405352	20.754808937084025	16.864177035897594
8	26.172383359440026	35.49837382091546	20.246253239222327	18.08298958042219
9	27.279559919403845	17.6425167565987	19.05439631316754	36.02352701082992
10	13.222644650906782	32.766192965456995	31.991717788838685	22.019444594797548
11	33.29711149219771	26.16387095988133	21.8218951510961	18.717122396824866
12	21.932780340187495	29.496419860540946	29.114913986035816	19.45588581323574
13	26.84200467368354	24.700851511941956	26.0144017677458	22.442742046628712
14	20.436522742952175	23.7746736701994	28.276890321814257	27.511913265034167
15	22.647554870272018	31.44659547332153	23.596748710334456	22.309100946071993
16	22.005138334503442	31.36493292295696	24.514987824248855	22.114940918290735
17	21.33609875731044	30.700546156007373	25.864556660354616	22.09879842632757
18	20.7131147603395	29.493420196034986	28.33941326559748	21.45405177802803
19	22.141993470024243	28.69740080162762	28.21305873323344	20.947546995114703
20	22.307631857429406	28.468798948940126	28.099676731948453	21.12389246168202
21	23.137187716533035	27.843007363074978	27.97884733992736	21.040957580464628
22	22.628569253026726	28.21008147414909	27.507271758166453	21.654077514657732
23	21.952775762153454	28.27835057041111	28.02495296383069	21.74392070360475
24	21.815376954137776	28.330552812260386	27.916330047216825	21.937740186385017
25	21.64311095308323	28.188967505221136	28.611226798009152	21.556694743686478
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12915.0
1	12915.0
2	12250.0
3	11585.0
4	11585.0
5	11585.0
6	14264.0
7	16943.0
8	16943.0
9	16943.0
10	15709.0
11	14475.0
12	14475.0
13	14475.0
14	14794.5
15	15114.0
16	15114.0
17	15114.0
18	19122.0
19	23130.0
20	23130.0
21	23130.0
22	29972.5
23	36815.0
24	36815.0
25	36815.0
26	48468.5
27	60122.0
28	60122.0
29	60122.0
30	72446.0
31	84770.0
32	84770.0
33	84770.0
34	93951.0
35	103132.0
36	103132.0
37	103132.0
38	113097.0
39	123062.0
40	123062.0
41	123062.0
42	132917.5
43	142773.0
44	142773.0
45	142773.0
46	149861.5
47	156950.0
48	156950.0
49	156950.0
50	153199.0
51	149448.0
52	149448.0
53	149448.0
54	139601.0
55	129754.0
56	129754.0
57	129754.0
58	113638.5
59	97523.0
60	97523.0
61	97523.0
62	81309.5
63	65096.0
64	65096.0
65	65096.0
66	51026.5
67	36957.0
68	36957.0
69	36957.0
70	28457.5
71	19958.0
72	19958.0
73	19958.0
74	14462.0
75	8966.0
76	8966.0
77	8966.0
78	6813.0
79	4660.0
80	4660.0
81	4660.0
82	3232.5
83	1805.0
84	1805.0
85	1805.0
86	1301.5
87	798.0
88	798.0
89	798.0
90	532.5
91	267.0
92	267.0
93	267.0
94	149.5
95	32.0
96	32.0
97	32.0
98	25.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3223827471322665
2	0.3232938618203464
3	0.3365809510215114
4	0.33680872969353143
5	0.3433383849581039
6	0.28548260226503114
7	0.29520115893788323
8	0.2936067082337434
9	0.2926196673216569
10	0.4055219624195562
11	0.29337892956172346
12	0.2923918886496369
13	0.28525482359301113
14	0.29049373304947046
15	0.2841918564569179
16	0.28867150367331074
17	0.2814585123926783
18	0.28328074176883805
19	0.28396407778489796
20	0.48524449762654626
21	0.2853307498170178
22	0.28609001205708434
23	0.28221777463274483
24	0.2858622333850644
25	0.2776622011923454
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1317068.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.10907573431139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.96477949357413	51.921191448218885
2	7.979580840417502	9.752496198102664
3	2.7263497070435294	4.998141321778221
4	1.2946052734721385	3.1644852681059126
5	0.7405015165560107	2.2625681628296848
6	0.4679589888464727	1.7157924773982525
7	0.2999716826518187	1.2831694591323166
8	0.2339948150860322	1.1439365501222825
9	0.18140556643422995	0.9976973848069512
>10	0.9850624340868537	11.100227346056176
>50	0.08471522809321223	3.594881502530503
>100	0.038140575321564854	4.129994096510072
>500	0.0015307191738062958	0.6401311067931593
>1k	0.0011480393803547217	2.0794127380436334
>5k	2.551198623010493E-4	1.2158749395713224
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	8347	0.6337561917835678	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7251	0.5505410502722715	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4125	0.31319567402746096	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3960	0.30066784706636257	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3808	0.2891270610173507	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3459	0.2626288088390273	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2988	0.22686755733189173	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	2377	0.1804766344638242	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2312	0.17554142990339147	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2183	0.1657469470065327	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	1464	0.11115599194574616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	7.592622400665721E-5	0.0	0.0	0.0	0.0
6	7.592622400665721E-5	0.0	0.0	0.0	0.0
7	1.5185244801331442E-4	0.0	0.0	0.0	0.0
8	1.5185244801331442E-4	0.0	0.0	0.0	0.0
9	1.5185244801331442E-4	0.0	0.0	0.0	0.0
10	1.5185244801331442E-4	0.0	0.0	0.0	0.0
11	1.5185244801331442E-4	0.0	0.0	0.0	0.0
12	1.5185244801331442E-4	0.0	0.0	0.0	0.0
13	1.5185244801331442E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCCGA	50	4.5214347E-6	17.115728	19
TAACGCA	140	0.0	16.289507	4
AATGCGT	55	1.1402844E-5	15.527758	12
TATCACG	50	8.75458E-5	15.191373	2
TATAACG	125	0.0	15.191373	2
ATAACGC	145	0.0	14.405612	3
CGCATAT	80	1.2920282E-7	14.246255	8
ATGCGTA	60	2.5944564E-5	14.234319	13
GAATGCG	60	2.5953883E-5	14.233776	11
GGTATCA	1475	0.0	13.00229	1
GTATAAC	220	0.0	12.515143	1
TTATACC	130	1.9645086E-10	12.425969	4
ATTATAC	180	0.0	12.132	3
GTATAAA	250	0.0	11.772865	1
ATCGTAG	65	8.0963963E-4	11.6794405	13
TAGACTA	90	7.438657E-6	11.613815	5
CTTACAC	190	0.0	11.493474	3
ACGCCCC	75	2.0549838E-4	11.410922	17
AGGGACG	75	2.0688662E-4	11.402654	5
GTTATCA	225	0.0	11.393096	1
>>END_MODULE