FastQCFastQC Report
Thu 26 May 2016
SRR1364322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364322_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317068
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT189391.4379667564620808No Hit
GTACTTTTTTTTTTTTTTTTTTTTT143081.0863524130872513No Hit
TATCAACGCAGAGTACTTTTTTTTT135801.0310781220104048No Hit
GGTATCAACGCAGAGTACTTTTTTT131020.9947853869352229No Hit
GAGTACTTTTTTTTTTTTTTTTTTT89470.6793119261875621No Hit
ACGCAGAGTACTTTTTTTTTTTTTT86000.652965526457252No Hit
GCAGAGTACTTTTTTTTTTTTTTTT28680.2177564104510929No Hit
GTGGTATCAACGCAGAGTACTTTTT24070.1827544211840239No Hit
ATCAACGCAGAGTACTTTTTTTTTT22280.16916362708683227No Hit
GTATAACGCAGAGTACTTTTTTTTT16860.12801161367522407No Hit
GTGGTATCAACGCAGAGTACATGGG15680.1190523192424385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1950.015.5893092
GACGAAA755.8504156E-815.1984211
TCGCGGT755.869515E-815.19438117
CGAAATC508.739851E-515.1943813
ACGAAAT508.745514E-515.19322712
AGTAGTC456.735572E-414.7852265
ATAACGC2100.014.4774353
CAACCCG602.5619162E-514.25339110
CTTCGCG801.293738E-714.24473115
GGTATCA28700.014.2352131
TAACGCA2150.014.14289954
ATCACGC1350.014.0752843
CGCTCGG551.9514331E-413.82514819
TTAGTAC551.9583646E-413.819373
CGCGGTT1001.0117219E-813.29508218
GTGGTAT7050.013.2073111
TATCACG1450.013.1030822
GTATAAC2700.013.0201321
TCACGCA1501.8189894E-1212.6696824
TTCGCGG1052.0027073E-812.66198416
Produced by FastQC (version 0.11.5)