##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364322_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1317068 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.323065323886087 31.0 30.0 34.0 25.0 34.0 2 29.32803317672284 31.0 30.0 34.0 25.0 34.0 3 29.367190608229794 31.0 29.0 34.0 25.0 34.0 4 32.67782377219703 35.0 32.0 37.0 26.0 37.0 5 32.26087035749104 35.0 32.0 37.0 23.0 37.0 6 32.121438680462965 35.0 32.0 37.0 22.0 37.0 7 31.824050087011454 35.0 32.0 37.0 18.0 37.0 8 31.7032256497007 35.0 32.0 37.0 17.0 37.0 9 32.36424087442714 37.0 32.0 39.0 10.0 39.0 10 32.52757184898578 37.0 32.0 39.0 15.0 39.0 11 32.45029489745404 37.0 32.0 39.0 11.0 39.0 12 32.33978579693683 37.0 32.0 39.0 10.0 39.0 13 32.41045488919327 37.0 32.0 39.0 11.0 39.0 14 33.30541627311574 38.0 32.0 41.0 10.0 41.0 15 33.32902781025733 38.0 32.0 41.0 10.0 41.0 16 33.298869914081884 38.0 32.0 41.0 10.0 41.0 17 33.283558631748704 38.0 32.0 41.0 10.0 41.0 18 33.242851546009774 38.0 32.0 41.0 10.0 41.0 19 33.15254185812729 38.0 32.0 41.0 8.0 41.0 20 32.97016858658778 38.0 32.0 41.0 2.0 41.0 21 32.88466047311149 38.0 32.0 41.0 2.0 41.0 22 32.84875344325426 38.0 32.0 41.0 2.0 41.0 23 32.527426070635684 38.0 32.0 40.0 2.0 41.0 24 32.42448529612746 38.0 32.0 41.0 2.0 41.0 25 32.27722410687983 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.46774553511544426 1101 2 0.5414606332989891 1101 3 -1.2802359924181346 1101 4 -0.1621290706446672 1101 5 0.7117839582170262 1101 6 0.958329913858627 1101 7 1.0947679527123526 1101 8 1.3620345194420267 1101 9 1.9872043323770185 1101 10 1.661731495749578 1101 11 1.6010123297226997 1101 12 1.9304348685084491 1101 13 1.6118809450661615 1101 14 1.8931137498082293 1101 15 1.863974772817599 1101 16 1.7243078249843151 1101 17 1.6080788179246923 1101 18 1.6082445137832266 1101 19 1.6866031217166153 1101 20 2.104645922342634 1101 21 1.9961267705031176 1101 22 2.200664165411439 1101 23 2.416170957511575 1101 24 2.3416867618653043 1101 25 2.304115775355914 1102 1 0.7879883348186318 1102 2 0.7259288726128688 1102 3 0.9362273345302761 1102 4 1.034177164147998 1102 5 1.1691185685352963 1102 6 1.1770466590426096 1102 7 1.327232418593269 1102 8 1.4162229725070787 1102 9 1.9841801214329138 1102 10 1.65473108734448 1102 11 1.633609958672288 1102 12 1.792474478050174 1102 13 1.6262197027093777 1102 14 1.6876773603393218 1102 15 1.7398915272050743 1102 16 1.7137913938695988 1102 17 1.6870821944569272 1102 18 1.5343533911294003 1102 19 1.5783671441041136 1102 20 1.8337151613861593 1102 21 2.0129619761724697 1102 22 1.9871430807795534 1102 23 2.3048935124017973 1102 24 2.2204091441899934 1102 25 2.200547695377548 1103 1 -1.4367409464668484 1103 2 -1.651789230609559 1103 3 -1.270686908767253 1103 4 -1.2078104351883034 1103 5 -4.175305499231712 1103 6 -8.866202838817838 1103 7 -9.755871677118897 1103 8 -9.605303531728357 1103 9 -10.758145876296542 1103 10 -8.737538146783539 1103 11 -10.77655072598407 1103 12 -10.250589446497905 1103 13 -11.08114209975329 1103 14 -10.889117242752107 1103 15 -10.922452372890984 1103 16 -4.821260130014775 1103 17 -3.014199353006177 1103 18 -2.132728086470813 1103 19 -1.7237352923643137 1103 20 -7.241049051346355 1103 21 -3.9563092665137596 1103 22 -9.706748282420168 1103 23 -11.786015578507293 1103 24 -12.154525359346813 1103 25 -12.608082555255589 1104 1 0.6716314595416613 1104 2 0.8191016263844517 1104 3 0.8919096875685959 1104 4 1.0935701326679066 1104 5 1.135322268416111 1104 6 1.1066850660184926 1104 7 0.9865417117844615 1104 8 1.1478439893274768 1104 9 1.5895683722056688 1104 10 1.3111833084565419 1104 11 1.3007046356552436 1104 12 1.138042575730104 1104 13 1.1837272238552927 1104 14 1.1891692033710157 1104 15 1.2881493463067244 1104 16 0.9654573311296204 1104 17 1.0096024182884307 1104 18 1.0087801187909733 1104 19 1.3948828373569668 1104 20 1.5749946232216772 1104 21 1.2698672551498689 1104 22 1.1138864314810206 1104 23 1.4944828151716152 1104 24 1.7268348038901422 1104 25 1.8333409925433166 1105 1 0.5480964950032465 1105 2 0.5261195952584501 1105 3 0.2567337997759047 1105 4 0.5435368569753152 1105 5 0.6893026817029977 1105 6 0.9023500368778983 1105 7 0.9374426616036047 1105 8 0.8598435660718025 1105 9 1.0175299784609422 1105 10 0.5691033409803978 1105 11 1.206111169943128 1105 12 1.1801133541028577 1105 13 1.0738185858196445 1105 14 0.9135044552777529 1105 15 0.6956234288582834 1105 16 0.9494682912238517 1105 17 1.002089630119542 1105 18 1.0300292242456806 1105 19 1.1305694165322322 1105 20 1.2795468493515898 1105 21 1.2243714473302205 1105 22 0.7190181280401191 1105 23 1.3792375357254159 1105 24 1.3603942448585045 1105 25 1.4298293627583725 1106 1 0.27138982650349774 1106 2 0.4297615715294789 1106 3 0.22054222751535946 1106 4 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-3.4234228666817437 2216 18 -2.8903845598807116 2216 19 -4.373561176779241 2216 20 -3.1233941922531407 2216 21 -3.535301241053677 2216 22 -3.2147622116669723 2216 23 -2.987090252184707 2216 24 -3.004457327429467 2216 25 -3.012105663045819 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55774.0 3 5.0 4 735.0 5 7735.0 6 10733.0 7 8369.0 8 6487.0 9 5432.0 10 4521.0 11 4111.0 12 3608.0 13 3260.0 14 3322.0 15 3410.0 16 3434.0 17 3721.0 18 4172.0 19 5507.0 20 7225.0 21 9230.0 22 11223.0 23 13064.0 24 14879.0 25 16600.0 26 18591.0 27 20182.0 28 22445.0 29 25899.0 30 31341.0 31 38653.0 32 47780.0 33 58798.0 34 72872.0 35 91308.0 36 119173.0 37 178321.0 38 385148.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.69624944558871 18.6832807781808 11.746304431037311 23.874165345193177 2 16.745403356597482 21.84210446392235 36.92158524214759 24.49090693733258 3 19.27587531680265 27.580470530709928 28.896122138146456 24.247532014340965 4 12.074860536260571 16.69601346687836 35.58859506654852 35.64053093031255 5 12.269064058639596 39.48223260038902 33.67986857585104 14.568834765120348 6 30.771987747335107 37.066285580642365 17.484346263833224 14.6773804081893 7 27.068876222067306 33.300299356179174 21.91124719590895 17.719577225844567 8 27.408807047095824 33.581471223270185 20.204353020070005 18.80536870956399 9 26.957711219841197 14.466654615551711 20.147970475781058 38.42766368882604 10 15.50555107007253 30.572621911251236 31.692102266987597 22.22972475168864 11 34.188195189237305 23.055141172217038 22.756785168486072 19.999878470059578 12 22.919851023360174 27.26910329274821 30.056302843236637 19.75474284065498 13 28.691611492437584 21.627209500091112 26.987638947943875 22.693540059527425 14 21.83112173461423 20.930967140370964 29.409884312480827 27.828026812533977 15 23.581701510194133 28.4035557026932 25.694105390882036 22.320637396230627 16 22.017602540378704 28.266420849693503 27.017568371009492 22.698408238918304 17 21.420734073273685 27.48108702436609 28.7136291120488 22.38454979031142 18 20.708542667776367 25.545383302249576 31.813722214459723 21.932351815514338 19 22.066735567471024 25.10697723160011 31.865812013191896 20.960475187736975 20 21.943585093926128 24.6778384765272 32.06587358359038 21.312702845956295 21 22.574675741328107 24.064355883051984 31.99736963400401 21.3635987416159 22 22.2458629380145 24.40595108194242 31.78268968067569 21.565496299367396 23 21.4129022607317 24.281927434559663 32.596891484422876 21.708278820285763 24 21.604633610380063 24.56442209712176 32.07004478407515 21.76089950842302 25 21.41849545267299 24.44277573157774 32.6120794881578 21.526649327591475 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1732.0 1 1732.0 2 2385.0 3 3038.0 4 3038.0 5 3038.0 6 10014.0 7 16990.0 8 16990.0 9 16990.0 10 15499.0 11 14008.0 12 14008.0 13 14008.0 14 11854.0 15 9700.0 16 9700.0 17 9700.0 18 14796.5 19 19893.0 20 19893.0 21 19893.0 22 29219.0 23 38545.0 24 38545.0 25 38545.0 26 53185.5 27 67826.0 28 67826.0 29 67826.0 30 78231.5 31 88637.0 32 88637.0 33 88637.0 34 97636.0 35 106635.0 36 106635.0 37 106635.0 38 112857.0 39 119079.0 40 119079.0 41 119079.0 42 127848.5 43 136618.0 44 136618.0 45 136618.0 46 145883.0 47 155148.0 48 155148.0 49 155148.0 50 153775.5 51 152403.0 52 152403.0 53 152403.0 54 142959.5 55 133516.0 56 133516.0 57 133516.0 58 117766.5 59 102017.0 60 102017.0 61 102017.0 62 86310.0 63 70603.0 64 70603.0 65 70603.0 66 55274.0 67 39945.0 68 39945.0 69 39945.0 70 31100.0 71 22255.0 72 22255.0 73 22255.0 74 15951.0 75 9647.0 76 9647.0 77 9647.0 78 7520.5 79 5394.0 80 5394.0 81 5394.0 82 3779.0 83 2164.0 84 2164.0 85 2164.0 86 1527.0 87 890.0 88 890.0 89 890.0 90 623.5 91 357.0 92 357.0 93 357.0 94 192.5 95 28.0 96 28.0 97 28.0 98 14.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027029735746369965 2 0.0015185244801331442 3 1.5185244801331442E-4 4 0.004479647216392776 5 0.03150938296276274 6 0.009870409120865438 7 0.018374146209611045 8 0.024827875250176906 9 0.03447050569902237 10 0.03606495640316217 11 0.03948163648346175 12 0.006301876592552549 13 0.002125934272186402 14 0.005466688128479319 15 0.006453729040565863 16 0.007516696176659064 17 0.00873151576076558 18 0.014881539905304813 19 0.015109318577324785 20 0.013818572769211612 21 0.010705597584938668 22 0.009946335344872093 23 0.007972253520699007 24 0.006225950368545892 25 0.10250040240898724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1317068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.91555326735589 #Duplication Level Percentage of deduplicated Percentage of total 1 73.53942633955099 31.55985168325795 2 12.783634779074923 10.972335186236265 3 5.0049622100588085 6.443721669806463 4 2.511308397886457 4.310967572810177 5 1.5141867794989494 3.2491081696156607 6 0.9258757313039826 2.384068155943689 7 0.6363748767035333 1.9117265943424266 8 0.4683085988261741 1.6078178094788378 9 0.34201414737494346 1.3209953723872825 >10 1.9951067085268812 15.876374021479588 >50 0.17875074398201554 5.354380209045328 >100 0.09451216972668552 7.11409698509938 >500 0.0032159133788050836 0.965858892209332 >1k 0.0012506329806464213 0.9883242931315622 >5k 3.5732370875612036E-4 1.34539382246544 >10k+ 7.146474175122407E-4 4.594979562690566 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 18939 1.4379667564620808 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 14308 1.0863524130872513 No Hit TATCAACGCAGAGTACTTTTTTTTT 13580 1.0310781220104048 No Hit GGTATCAACGCAGAGTACTTTTTTT 13102 0.9947853869352229 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 8947 0.6793119261875621 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 8600 0.652965526457252 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 2868 0.2177564104510929 No Hit GTGGTATCAACGCAGAGTACTTTTT 2407 0.1827544211840239 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2228 0.16916362708683227 No Hit GTATAACGCAGAGTACTTTTTTTTT 1686 0.12801161367522407 No Hit GTGGTATCAACGCAGAGTACATGGG 1568 0.1190523192424385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.592622400665721E-5 0.0 0.0 0.0 0.0 6 7.592622400665721E-5 0.0 0.0 0.0 0.0 7 7.592622400665721E-5 0.0 0.0 0.0 0.0 8 7.592622400665721E-5 0.0 0.0 0.0 0.0 9 7.592622400665721E-5 0.0 0.0 0.0 0.0 10 7.592622400665721E-5 0.0 0.0 0.0 0.0 11 7.592622400665721E-5 0.0 0.0 0.0 0.0 12 7.592622400665721E-5 0.0 0.0 7.592622400665721E-5 0.0 13 7.592622400665721E-5 0.0 0.0 7.592622400665721E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 195 0.0 15.589309 2 GACGAAA 75 5.8504156E-8 15.19842 11 TCGCGGT 75 5.869515E-8 15.194381 17 CGAAATC 50 8.739851E-5 15.19438 13 ACGAAAT 50 8.745514E-5 15.193227 12 AGTAGTC 45 6.735572E-4 14.785226 5 ATAACGC 210 0.0 14.477435 3 CAACCCG 60 2.5619162E-5 14.253391 10 CTTCGCG 80 1.293738E-7 14.244731 15 GGTATCA 2870 0.0 14.235213 1 TAACGCA 215 0.0 14.1428995 4 ATCACGC 135 0.0 14.075284 3 CGCTCGG 55 1.9514331E-4 13.825148 19 TTAGTAC 55 1.9583646E-4 13.81937 3 CGCGGTT 100 1.0117219E-8 13.295082 18 GTGGTAT 705 0.0 13.207311 1 TATCACG 145 0.0 13.103082 2 GTATAAC 270 0.0 13.020132 1 TCACGCA 150 1.8189894E-12 12.669682 4 TTCGCGG 105 2.0027073E-8 12.661984 16 >>END_MODULE