FastQCFastQC Report
Thu 26 May 2016
SRR1364321_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364321_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802213
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT133830.4775868215585325No Hit
TATCAACGCAGAGTACTTTTTTTTT83880.29933484713688785No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN77620.2769953604526137No Hit
GTACTTTTTTTTTTTTTTTTTTTTT54560.19470325774664524No Hit
GGTATCAACGCAGAGTACTTTTTTT53560.19113464965011584No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39550.1411384502177386No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30830.11002018761600207No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30270.10802176708194558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCG250.006032069719.0006478
CGAGACA653.3532397E-614.6242684
TAACGCA2300.014.4653074
CGCATAT1600.014.2504848
GGTATCA30000.012.7550141
ATAACGC2550.012.6605173
TATCACG1155.3696567E-912.3852882
ATCACGC1155.3696567E-912.3852883
TCGAGAC701.09645945E-412.2083563
TTACGGT550.003055561312.0982584
TATAACG2600.012.0518392
AGGTCGC802.8693828E-511.87455210
GTATAAC4550.011.6862131
AAGACCG1157.025483E-811.5718315
GTATCAA77300.011.3989711
CGGTTTG1104.932954E-711.23429814
TTGACCG3700.011.04705318
TGACCGA3550.010.97790819
TGCCCGA1303.2714524E-810.96112410
AACTGGC5350.010.8383617
Produced by FastQC (version 0.11.5)