##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364321_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2802213
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.36514925881794	30.0	26.0	31.0	10.0	34.0
2	26.20173270197519	31.0	26.0	31.0	7.0	34.0
3	25.63320489912794	30.0	25.0	31.0	7.0	34.0
4	29.416754900501854	35.0	28.0	37.0	10.0	37.0
5	29.9807612768908	35.0	30.0	37.0	10.0	37.0
6	30.25888074889382	35.0	31.0	37.0	7.0	37.0
7	29.785530578867487	35.0	30.0	37.0	2.0	37.0
8	29.30507174151287	35.0	29.0	37.0	2.0	37.0
9	30.406806691711157	35.0	28.0	39.0	2.0	39.0
10	29.711879503806458	35.0	27.0	39.0	2.0	39.0
11	30.376633039672573	35.0	29.0	39.0	2.0	39.0
12	29.900567872606402	35.0	27.0	39.0	2.0	39.0
13	29.51599753480553	35.0	27.0	39.0	2.0	39.0
14	29.82838670722033	36.0	27.0	40.0	2.0	41.0
15	30.54099277963524	36.0	27.0	40.0	2.0	41.0
16	30.904686403210604	37.0	29.0	40.0	2.0	41.0
17	31.084703768057604	37.0	30.0	40.0	2.0	41.0
18	30.915832236878497	37.0	29.0	40.0	2.0	41.0
19	30.915544250205105	37.0	29.0	40.0	2.0	41.0
20	30.785113765441814	37.0	29.0	40.0	2.0	41.0
21	30.70911026392355	37.0	29.0	40.0	2.0	41.0
22	30.787644265443063	37.0	29.0	40.0	2.0	41.0
23	30.790942016185067	37.0	30.0	40.0	2.0	41.0
24	30.6522166587622	37.0	30.0	40.0	2.0	41.0
25	30.469194525897926	37.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7824512837236597
1101	2	0.9034211068287199
1101	3	1.5863987385970333
1101	4	1.0085125074448236
1101	5	0.46643296827754455
1101	6	0.28716246441941706
1101	7	0.6855017508959875
1101	8	0.9311498505391036
1101	9	0.7814518909072063
1101	10	1.156788975073269
1101	11	0.4526885779056933
1101	12	0.7504397949399007
1101	13	0.7294831181801769
1101	14	1.2122569978861257
1101	15	0.46475507201967403
1101	16	-0.15259135733940354
1101	17	-0.7840266444930357
1101	18	-6.281864048508098
1101	19	-7.702067309632191
1101	20	-8.818087674787304
1101	21	-10.341080973072899
1101	22	-10.453808809078652
1101	23	-12.022450636849747
1101	24	-11.68412959497434
1101	25	-11.968179819409823
1102	1	2.1875503029549463
1102	2	2.3130094960744962
1102	3	2.963107226265855
1102	4	2.5271817302973005
1102	5	1.7897978912760202
1102	6	1.9154636503643516
1102	7	2.1604589325857404
1102	8	2.5050799972120785
1102	9	2.664089663662729
1102	10	-0.7005139301978467
1102	11	1.2955027120966562
1102	12	2.217221303144477
1102	13	2.7206501554718088
1102	14	3.3225189959420582
1102	15	2.790867729919924
1102	16	2.4334905625913166
1102	17	2.073583310784702
1102	18	2.3750039974658783
1102	19	2.1191026563933946
1102	20	2.362367009785853
1102	21	2.4029430084777275
1102	22	2.3000342338594493
1102	23	2.372966196721787
1102	24	2.546706127227825
1102	25	2.5406886397738973
1103	1	-1.5165011652073765
1103	2	-0.9412762182112182
1103	3	-3.484203892176904
1103	4	-1.5443062274724149
1103	5	-3.6837685884336473
1103	6	-8.434447270123936
1103	7	-10.245710648410633
1103	8	-3.853976419812007
1103	9	-8.490684338316818
1103	10	-6.6950569852951745
1103	11	-8.989947634323666
1103	12	-4.566559627241535
1103	13	-1.9715372414269048
1103	14	-1.827246757934546
1103	15	-2.2774331608751943
1103	16	-2.422576741928644
1103	17	-2.0302833998718484
1103	18	-2.1919310074829816
1103	19	-7.532009187882419
1103	20	-3.1970061343909855
1103	21	-7.248069858562854
1103	22	-4.6331759278033715
1103	23	-3.212682104169012
1103	24	-8.963825050601272
1103	25	-6.245071835316828
1104	1	1.8117159855080551
1104	2	1.8347835047840988
1104	3	2.6721171189229302
1104	4	2.1079998528785886
1104	5	1.7067451472004187
1104	6	1.6162740553074535
1104	7	1.6819668998032462
1104	8	2.0122755038316207
1104	9	2.3082718189799323
1104	10	2.619810242366004
1104	11	2.0344392554310886
1104	12	2.3806339813501936
1104	13	2.639383160655541
1104	14	3.1734094091850658
1104	15	2.2782238248161732
1104	16	1.4561777192874246
1104	17	1.2892058113524065
1104	18	1.3624935365725541
1104	19	1.7783316653967773
1104	20	-8.63394795031057
1104	21	-2.8701870125525737
1104	22	-0.503338659868465
1104	23	0.5413469060140237
1104	24	1.1039631685140137
1104	25	1.6675780451716946
1105	1	1.4163076362595355
1105	2	1.4123093793883825
1105	3	2.2347492666829076
1105	4	1.600871302115852
1105	5	1.3358546821114423
1105	6	1.3105302261254401
1105	7	1.542487384594871
1105	8	1.7542916248621054
1105	9	1.8004108442136015
1105	10	2.190530612637815
1105	11	1.87119588959699
1105	12	2.066239172558433
1105	13	2.037877661056843
1105	14	2.7746135521171276
1105	15	2.0402997946173898
1105	16	1.5138393274162496
1105	17	0.9201216676394033
1105	18	1.3543133665793654
1105	19	1.1838901286703383
1105	20	1.2455076179010618
1105	21	1.0979678143826348
1105	22	1.2022033020682734
1105	23	1.1074379158343248
1105	24	1.1771784499821578
1105	25	1.1077136289272111
1106	1	0.8639023860436339
1106	2	1.1628211721573578
1106	3	1.6059292797359284
1106	4	1.4166057983548654
1106	5	0.6165502170546482
1106	6	0.5499716160746111
1106	7	0.9577195372229426
1106	8	0.8953577206238315
1106	9	1.2900635766683841
1106	10	-2.36009061456609
1106	11	-0.2320851107831743
1106	12	0.414979248195646
1106	13	1.484257754537584
1106	14	0.5575339792190732
1106	15	1.1838837613739308
1106	16	0.8401297417947902
1106	17	-5.862375140522911
1106	18	-1.8439595251047187
1106	19	-0.3813661962224799
1106	20	0.5316917402390899
1106	21	0.7660988258600661
1106	22	0.816701661650491
1106	23	0.3237316703803579
1106	24	0.6559866254815887
1106	25	0.7467912262919967
1107	1	0.9007128897731782
1107	2	1.217947197966513
1107	3	1.6615077565804306
1107	4	1.2035492672866361
1107	5	0.6993891675159851
1107	6	0.7677559074091924
1107	7	0.7070416754076838
1107	8	0.9102664439232449
1107	9	1.1268105525790872
1107	10	-2.169264909350815
1107	11	-0.05127666062731251
1107	12	0.28967084279604194
1107	13	1.42902179344685
1107	14	1.7171767373518918
1107	15	1.467828886721879
1107	16	-5.501002478628493
1107	17	-1.7022340887768515
1107	18	0.04230460462065011
1107	19	0.8340057109243553
1107	20	1.1779849658793005
1107	21	1.3146092897000514
1107	22	0.9726477916244107
1107	23	0.6620097014872606
1107	24	1.0483300024674875
1107	25	1.1437250530739682
1108	1	-0.6526604157928553
1108	2	-3.429145070670234
1108	3	-0.46757625751648035
1108	4	-0.9586856185731172
1108	5	-1.161145637613977
1108	6	-0.6832528907328346
1108	7	-0.4714717725324107
1108	8	-0.21556892566680474
1108	9	-0.7814571720404722
1108	10	-3.954494924405246
1108	11	-1.8770670722627756
1108	12	-6.20876103239376
1108	13	-2.1461508245650798
1108	14	-5.863827554187473
1108	15	-2.8344823412030635
1108	16	-7.576487748954406
1108	17	-3.507004711347257
1108	18	-2.095537564860031
1108	19	-0.9220794454936616
1108	20	-0.8290436051169827
1108	21	-0.433924659499624
1108	22	-0.6413258107073503
1108	23	-0.5824713313352881
1108	24	-0.3894924979080656
1108	25	-0.6399664488999655
1109	1	-8.944438387822672
1109	2	-6.707701895190699
1109	3	-5.503056403915501
1109	4	-5.2167279165442615
1109	5	-2.526113735407339
1109	6	-1.6306034417326067
1109	7	-1.22452328107288
1109	8	-0.8161298899348601
1109	9	-5.785909140529462
1109	10	-1.635648739331323
1109	11	-1.2347472741255565
1109	12	-6.339308067455082
1109	13	-8.032318099470256
1109	14	-8.193575953277701
1109	15	-3.9726193704333106
1109	16	-2.3811423816018937
1109	17	-1.9505033780026757
1109	18	-1.2647386231001398
1109	19	-0.990776795781052
1109	20	-0.7335649560286015
1109	21	-0.6439893285570655
1109	22	-0.8321133433819377
1109	23	-1.1610258360787036
1109	24	-0.8591784949956889
1109	25	-0.7441661943571596
1110	1	-2.6484381893395224
1110	2	-2.486222662913761
1110	3	-1.7508124868342776
1110	4	-2.5425485813245245
1110	5	-5.947864600822342
1110	6	-4.38039255338629
1110	7	-9.423372282169826
1110	8	-10.205079787988545
1110	9	-11.757637493562655
1110	10	-9.368622606665674
1110	11	-12.268312584639837
1110	12	-6.828869602722243
1110	13	-5.087722555660999
1110	14	-4.653442396081406
1110	15	-9.862640284375
1110	16	-11.514516347038981
1110	17	-12.363322501691435
1110	18	-9.079268728479239
1110	19	-12.12444950276176
1110	20	-13.36611956838247
1110	21	-14.511474168796433
1110	22	-15.023672766105086
1110	23	-15.636065766577063
1110	24	-16.25096145529865
1110	25	-16.915337676070475
1111	1	-0.891616866106208
1111	2	-0.11804806751318608
1111	3	-2.807535313098686
1111	4	-3.6010372786612947
1111	5	-1.4287638074047706
1111	6	-0.7548361271392992
1111	7	-0.3159344005620959
1111	8	-0.056953407126343336
1111	9	-4.6439360754239765
1111	10	-4.463239556851239
1111	11	-1.6205589685494566
1111	12	-6.03292158300108
1111	13	-1.3681178495737782
1111	14	-0.11323005620700144
1111	15	-0.15177242268433844
1111	16	-0.2433340569028246
1111	17	-0.16959365677246652
1111	18	0.05128056898160693
1111	19	0.1797483183329156
1111	20	0.1864974035703817
1111	21	0.314398427278924
1111	22	-0.0783521884013183
1111	23	-0.16894525199842292
1111	24	0.017576109595069056
1111	25	0.19849162487313876
1112	1	-0.25193012091877875
1112	2	0.3501088011630422
1112	3	-2.517911901271596
1112	4	-0.43687483796973936
1112	5	-0.43206461430869325
1112	6	-0.2830154963384288
1112	7	0.2091329164531075
1112	8	-3.3746988715364736
1112	9	-0.813346137400945
1112	10	-3.588389037685733
1112	11	-1.0718126870024385
1112	12	0.19141520223295672
1112	13	0.6390446268281309
1112	14	1.339079818425656
1112	15	0.7365303963028857
1112	16	0.5619840548177635
1112	17	0.2291856493483806
1112	18	0.34583951161907933
1112	19	0.5209782909019651
1112	20	0.5458100182973915
1112	21	0.4536703021954622
1112	22	0.4129034266014706
1112	23	0.24892483854082315
1112	24	0.5472083499815561
1112	25	0.5571275434259242
1113	1	-0.7042601067000369
1113	2	-0.12946517831045412
1113	3	-2.6093537784700622
1113	4	-0.8572460662154349
1113	5	-1.012482667408218
1113	6	-0.8523591990054733
1113	7	-0.6276312603601859
1113	8	-8.342264614974297
1113	9	-9.064657875633337
1113	10	-2.8400290754336908
1113	11	-8.848775420141322
1113	12	-6.68384720218468
1113	13	-2.2304601275133216
1113	14	-0.49165031385764024
1113	15	-6.586769526391013
1113	16	-2.4468107712856835
1113	17	-1.5911393022907028
1113	18	-1.007827224146201
1113	19	-0.6455940545353052
1113	20	-6.500961065133183
1113	21	-2.155049391331101
1113	22	-1.4948112389466424
1113	23	-1.39846874878344
1113	24	-7.114230260442085
1113	25	-4.126159286216147
1114	1	-4.461972712266011
1114	2	-4.309160420984458
1114	3	-1.0893084491308223
1114	4	-0.7522749724687294
1114	5	-0.7360323345507886
1114	6	-0.9279381594184564
1114	7	-0.5346465052012306
1114	8	-0.163535455262096
1114	9	-0.5192416765177335
1114	10	-3.780498430198623
1114	11	-1.3578472084101918
1114	12	-4.756802686132879
1114	13	-1.2641160994664205
1114	14	-5.871186937907005
1114	15	-2.085482605266268
1114	16	-1.168345467710818
1114	17	-1.0032734405417791
1114	18	-0.664696242696099
1114	19	-0.372767506004557
1114	20	-0.15560222111081146
1114	21	-0.027387756535762975
1114	22	-0.1573722963306068
1114	23	-0.5638973986257305
1114	24	-0.44337672063041467
1114	25	-0.25076306406118576
1115	1	0.5614562711918438
1115	2	0.7316738796361406
1115	3	1.4009722330677157
1115	4	0.25625394865283013
1115	5	0.1825450123491308
1115	6	-0.4805419374429185
1115	7	-2.491925178743312
1115	8	-0.29625259398030224
1115	9	-0.011431647514470455
1115	10	0.7421323493427892
1115	11	-0.2036462644606516
1115	12	0.44874614575259386
1115	13	0.7603695589644843
1115	14	1.1938136628090916
1115	15	0.27592667278429417
1115	16	-0.06724528400300755
1115	17	-0.40620438813016335
1115	18	-0.10842684897026089
1115	19	0.12345436397668053
1115	20	0.24987620416087353
1115	21	0.24751111012599125
1115	22	-0.05973414698145163
1115	23	0.01735703477423911
1115	24	-0.142027105395794
1115	25	0.09395580656184066
1116	1	0.461458018466093
1116	2	0.6597441899520469
1116	3	1.4319185568026889
1116	4	0.7063060174255469
1116	5	-0.045197159862219394
1116	6	-0.9928146170627095
1116	7	-4.305506566777979
1116	8	-0.7674458075671069
1116	9	-0.871908828112737
1116	10	-0.07403657713190981
1116	11	-1.805866001670605
1116	12	-0.24676370887418742
1116	13	-6.282761731222823
1116	14	-1.3817365538529138
1116	15	-1.0806984669976458
1116	16	-0.5284951092755819
1116	17	-0.3504874815045014
1116	18	-0.050094272789102945
1116	19	0.21819489375023338
1116	20	0.3274836615273813
1116	21	0.39539952919224675
1116	22	0.2202934599517299
1116	23	0.14017503868813108
1116	24	0.32290964327627947
1116	25	0.25615265447908797
1201	1	2.3128173607895945
1201	2	2.5743758093842715
1201	3	3.1334976053832513
1201	4	2.8388403315045316
1201	5	2.3620399257847637
1201	6	2.1115799888361835
1201	7	2.490987988641365
1201	8	2.6419683892070083
1201	9	2.930241120205814
1201	10	3.1679886145702163
1201	11	2.8185329310473577
1201	12	3.1051078807510635
1201	13	3.1586932818778237
1201	14	3.713039292881536
1201	15	3.186467226134731
1201	16	2.859927044038038
1201	17	2.7125333593406715
1201	18	2.75251455872937
1201	19	2.6394126529387165
1201	20	2.913692166130634
1201	21	2.593888748201408
1201	22	1.75697719351583
1201	23	1.4184469552285996
1201	24	1.8511101673021422
1201	25	2.1001948650028766
1202	1	2.2258974157471947
1202	2	2.389233915481256
1202	3	3.055972831536266
1202	4	2.6696950207619885
1202	5	2.0352683001778544
1202	6	1.930918823887822
1202	7	2.3311247488892413
1202	8	2.5322404444917765
1202	9	2.822299270394545
1202	10	-0.83754359962359
1202	11	1.465145490532624
1202	12	2.5078587940704224
1202	13	3.0608761212508853
1202	14	3.5753062099069943
1202	15	3.067025663813567
1202	16	2.8197423462032774
1202	17	2.2637730334625807
1202	18	2.4993162414740873
1202	19	2.73829137382306
1202	20	2.704852420941929
1202	21	2.8156282001041646
1202	22	2.5468746585383144
1202	23	2.2958551621976433
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2211	16	-0.14583323052537978
2211	17	0.2603736380567412
2211	18	0.75549484814767
2211	19	0.9467415450744028
2211	20	1.15965466862108
2211	21	1.2016218366505527
2211	22	0.9880900043634036
2211	23	0.9380261635839737
2211	24	1.2355873494851402
2211	25	1.1183179742687024
2212	1	0.8758940169850327
2212	2	0.9436391652313816
2212	3	1.5903806481493667
2212	4	1.1409384744638587
2212	5	0.635414213822795
2212	6	0.4570483847014941
2212	7	0.6810464792002833
2212	8	1.0307316149493921
2212	9	0.8729198696417129
2212	10	1.6287179804008147
2212	11	0.8373736036699313
2212	12	1.1614415924565904
2212	13	1.3130915129563867
2212	14	-4.181239744670329
2212	15	-0.911633496292179
2212	16	-0.4429213066488735
2212	17	0.022175816472106646
2212	18	0.3631414120931744
2212	19	0.7634138949045912
2212	20	0.9775387657462282
2212	21	1.034607378820894
2212	22	0.8924590775328092
2212	23	0.7346879217495754
2212	24	0.8797608618853872
2212	25	0.9013468260644792
2213	1	0.6125335838273749
2213	2	0.7190584021234017
2213	3	1.285937677964668
2213	4	0.8155307523645661
2213	5	0.2100023253372676
2213	6	-0.12330183897850233
2213	7	0.15806455536457165
2213	8	-5.365259131094717
2213	9	-1.8325033001357802
2213	10	0.022557772319583336
2213	11	-0.06682451120054367
2213	12	0.48420828352637457
2213	13	-8.252566842456506
2213	14	-2.5391129098007
2213	15	-1.0236279270699633
2213	16	-0.5194707788226793
2213	17	-0.409596999185446
2213	18	-0.11669291224488632
2213	19	0.2395137636405309
2213	20	0.5137446163597659
2213	21	0.4199781894851924
2213	22	0.01086525623781398
2213	23	0.031333339846433717
2213	24	0.1511985444223214
2213	25	0.35460212435713245
2214	1	-0.8818651035945706
2214	2	-0.6267368041369217
2214	3	-6.931440376622689
2214	4	-2.5610443730477677
2214	5	-1.6594919348546675
2214	6	-1.1514572369013436
2214	7	-0.6725303403466008
2214	8	-6.298977929929794
2214	9	-2.0455861806605213
2214	10	-0.186280180704415
2214	11	-0.5444930888691211
2214	12	-0.036556607005955755
2214	13	-6.158785806815004
2214	14	-1.5568329856603107
2214	15	-0.7850381140615426
2214	16	-0.7536730874435946
2214	17	-0.5759433213457221
2214	18	-0.55732514822596
2214	19	-0.2185328967317126
2214	20	0.07776281758726711
2214	21	0.09452150356339217
2214	22	0.04183464302115425
2214	23	-0.0630898359720895
2214	24	0.14116437857420294
2214	25	0.17599128760675953
2215	1	0.4657037038785248
2215	2	0.754442660980331
2215	3	-2.275246611515442
2215	4	-0.387778896282704
2215	5	0.12420568809368504
2215	6	-0.406766976141391
2215	7	0.2960440599035614
2215	8	0.5113887616303465
2215	9	0.6724943479486853
2215	10	1.4330642825045494
2215	11	0.6260836013217173
2215	12	0.3260816221842795
2215	13	0.8847512289451664
2215	14	1.086570421670416
2215	15	0.7662232470255859
2215	16	0.3653791606896384
2215	17	0.4062990530450108
2215	18	0.6148948997287782
2215	19	0.5337418456499634
2215	20	0.7556443019893528
2215	21	0.9203913265542774
2215	22	0.7638934337032914
2215	23	0.569862682234298
2215	24	0.4914069200004718
2215	25	0.5122915087346165
2216	1	0.1336557333237245
2216	2	-0.09941580786121307
2216	3	1.048462205376893
2216	4	-0.3524225619930341
2216	5	-0.5069683764881638
2216	6	-0.7879405437985412
2216	7	-1.3428758775766845
2216	8	-6.358518529258298
2216	9	-2.3543468740090248
2216	10	-0.8957234360914406
2216	11	-1.4874955428338659
2216	12	-0.3833315797892958
2216	13	0.347903457993187
2216	14	0.9731783105061815
2216	15	0.4697606220573043
2216	16	-0.20361821848217332
2216	17	-0.31284440779036515
2216	18	-0.1484209547909785
2216	19	0.036467453959151186
2216	20	0.18956358684182817
2216	21	-0.025217425605333688
2216	22	-0.18358186227689188
2216	23	-0.4653446692980907
2216	24	-0.5175938534036639
2216	25	-0.5000705106575616
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	251159.0
3	4.0
4	1642.0
5	11107.0
6	16982.0
7	15082.0
8	13245.0
9	11091.0
10	9928.0
11	9359.0
12	8677.0
13	8406.0
14	8543.0
15	8403.0
16	8843.0
17	9491.0
18	10781.0
19	14114.0
20	20031.0
21	24916.0
22	30646.0
23	36134.0
24	42029.0
25	47461.0
26	52976.0
27	57976.0
28	63307.0
29	73485.0
30	84032.0
31	95439.0
32	118793.0
33	149778.0
34	187834.0
35	234767.0
36	291973.0
37	340817.0
38	432962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.77316693527971	17.948746410784246	12.970268277652671	23.30781837628337
2	14.343124515027306	21.383164505244597	40.96426268215906	23.30944829756904
3	15.82647478242559	28.1059011351615	30.303920171266213	25.763703911146703
4	10.564377238970469	17.020884941046784	37.27265597273071	35.14208184725203
5	12.535358057410956	39.783679243593646	32.93336676038178	14.747595938613614
6	30.451793903348705	37.164954062501344	18.523077561854908	13.860174472295043
7	28.481139758195145	32.98934161423982	21.232578327054043	17.296940300510993
8	26.56831349875394	34.44225850030391	20.597893904867043	18.39153409607511
9	27.773790522200027	16.377227707532477	19.512403785335387	36.33657798493211
10	13.277089341151873	31.351737221914227	32.91560068390134	22.455572753032563
11	33.76937216207557	24.82865513927195	22.741625158619506	18.66034754003297
12	22.51575267221806	27.629487269893577	30.03737400457394	19.817386053314422
13	27.153126659899407	23.112994139973456	26.964748210708972	22.769130989418162
14	20.933775424698005	22.1369507221808	29.12716545960165	27.802108393519543
15	23.159204362953833	30.204800695232127	24.047178715531444	22.588816226282596
16	22.54368695726875	29.917171963031635	25.037118701096023	22.50202237860359
17	21.709737480520243	29.41727876829952	26.473428430101585	22.399555321078655
18	21.307298230518324	28.109979387007765	28.789216674081715	21.793505708392196
19	22.949337489141293	27.29271279217393	28.39689159118218	21.361058127502602
20	23.007430300809016	27.27392702819885	28.08441401253099	21.634228658461144
21	23.862240427457134	26.34958393943212	27.868183536257117	21.919992096853633
22	23.42333893631217	26.900884545228067	27.70818598071697	21.967590537742794
23	22.65388211140588	27.10115494800801	28.004124712050434	22.24083822853568
24	22.858324041942797	27.1870083989398	27.99078675856046	21.963880800556947
25	22.559937237071072	27.037594049547497	28.43547937978659	21.966989333594846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12556.0
1	12556.0
2	9896.5
3	7237.0
4	7237.0
5	7237.0
6	11579.0
7	15921.0
8	15921.0
9	15921.0
10	16968.0
11	18015.0
12	18015.0
13	18015.0
14	21333.0
15	24651.0
16	24651.0
17	24651.0
18	35391.5
19	46132.0
20	46132.0
21	46132.0
22	62652.5
23	79173.0
24	79173.0
25	79173.0
26	106326.5
27	133480.0
28	133480.0
29	133480.0
30	161723.5
31	189967.0
32	189967.0
33	189967.0
34	214429.0
35	238891.0
36	238891.0
37	238891.0
38	261237.0
39	283583.0
40	283583.0
41	283583.0
42	304452.5
43	325322.0
44	325322.0
45	325322.0
46	338383.5
47	351445.0
48	351445.0
49	351445.0
50	338518.0
51	325591.0
52	325591.0
53	325591.0
54	298926.5
55	272262.0
56	272262.0
57	272262.0
58	237339.0
59	202416.0
60	202416.0
61	202416.0
62	166635.5
63	130855.0
64	130855.0
65	130855.0
66	102024.0
67	73193.0
68	73193.0
69	73193.0
70	55730.5
71	38268.0
72	38268.0
73	38268.0
74	27824.5
75	17381.0
76	17381.0
77	17381.0
78	13392.0
79	9403.0
80	9403.0
81	9403.0
82	6629.5
83	3856.0
84	3856.0
85	3856.0
86	2808.0
87	1760.0
88	1760.0
89	1760.0
90	1230.0
91	700.0
92	700.0
93	700.0
94	405.5
95	111.0
96	111.0
97	111.0
98	77.5
99	44.0
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3380185589032668
2	0.3400883515992539
3	0.3509726062936686
4	0.3537918066899268
5	0.35910903275375566
6	0.30036974348488144
7	0.30825636737821144
8	0.3071500988682873
9	0.30654343549187735
10	0.41938282350413764
11	0.3061152025202938
12	0.3054728530629185
13	0.2986568115985473
14	0.30411678198623726
15	0.29712231011703966
16	0.3023681640189379
17	0.29573055295939316
18	0.29776465957441495
19	0.29876386984144315
20	0.49125459056824017
21	0.2993705332178532
22	0.30193993104735434
23	0.2969438797122132
24	0.3000485687561938
25	0.2928756664821696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2802213.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.50560417101105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.8228563068534	47.92966743129606
2	7.7216495655991535	8.726329478020103
3	2.773269211824098	4.701157570289527
4	1.367818444743932	3.091576304660343
5	0.7805428356425075	2.2052522254667033
6	0.47514404135845695	1.6108980675129285
7	0.3422851139459334	1.3538719013580829
8	0.24504886881973856	1.1077307507265728
9	0.18385608649426427	0.9350009323088462
>10	1.0939432860169518	11.65218289665077
>50	0.11150559722925381	4.405831690403898
>100	0.0762061834602917	7.982135690690717
>500	0.0043251504044547326	1.6479169567046321
>1k	0.0012265351893132703	1.1787875328716568
>5k	2.5821793459226744E-4	0.9834902048918683
>10k+	6.455448364806686E-5	0.4881703661474621
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	13383	0.4775868215585325	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8388	0.29933484713688785	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	7762	0.2769953604526137	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5456	0.19470325774664524	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5356	0.19113464965011584	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3955	0.1411384502177386	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3083	0.11002018761600207	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3027	0.10802176708194558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.568608096529421E-5	0.0
10	0.0	0.0	0.0	3.568608096529421E-5	0.0
11	0.0	0.0	0.0	3.568608096529421E-5	0.0
12	0.0	0.0	0.0	3.568608096529421E-5	0.0
13	0.0	0.0	0.0	3.568608096529421E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGCG	25	0.0060320697	19.000647	8
CGAGACA	65	3.3532397E-6	14.624268	4
TAACGCA	230	0.0	14.465307	4
CGCATAT	160	0.0	14.250484	8
GGTATCA	3000	0.0	12.755014	1
ATAACGC	255	0.0	12.660517	3
TATCACG	115	5.3696567E-9	12.385288	2
ATCACGC	115	5.3696567E-9	12.385288	3
TCGAGAC	70	1.09645945E-4	12.208356	3
TTACGGT	55	0.0030555613	12.098258	4
TATAACG	260	0.0	12.051839	2
AGGTCGC	80	2.8693828E-5	11.874552	10
GTATAAC	455	0.0	11.686213	1
AAGACCG	115	7.025483E-8	11.571831	5
GTATCAA	7730	0.0	11.398971	1
CGGTTTG	110	4.932954E-7	11.234298	14
TTGACCG	370	0.0	11.047053	18
TGACCGA	355	0.0	10.977908	19
TGCCCGA	130	3.2714524E-8	10.961124	10
AACTGGC	535	0.0	10.83836	17
>>END_MODULE