FastQCFastQC Report
Thu 26 May 2016
SRR1364321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364321_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802213
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT291981.0419621920246604No Hit
TATCAACGCAGAGTACTTTTTTTTT227820.8130002965513328No Hit
GGTATCAACGCAGAGTACTTTTTTT188450.6725041957909695No Hit
GTACTTTTTTTTTTTTTTTTTTTTT132050.4712346991467101No Hit
ACGCAGAGTACTTTTTTTTTTTTTT97270.34711850954941686No Hit
GAGTACTTTTTTTTTTTTTTTTTTT89510.3194261107203485No Hit
GTGGTATCAACGCAGAGTACATGGG36840.1314675222761439No Hit
ATCAACGCAGAGTACTTTTTTTTTT34980.12482991121659916No Hit
GCAGAGTACTTTTTTTTTTTTTTTT33580.11983385988145798No Hit
GTGGTATCAACGCAGAGTACTTTTT32230.11501623895114325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG551.9590512E-413.8198319
TATCACG1650.013.8181042
TATAACG3300.013.5302272
GGTATCA51200.013.4720711
TAACGCA3450.012.9438054
ATAACGC3750.012.6668223
GTAGGAC1051.9956133E-812.66682153
CGAATCG550.00307497112.08803616
CGCGCCA1750.011.94385510
TAGTCGG658.0021186E-411.6966275
GTATAAC4400.011.66027451
ACAACCG1657.2759576E-1211.5194055
ATGCGTA1900.011.49726713
GTGCACG3650.011.45137711
GTGGTAT14200.011.3743931
TAGACAG3850.011.3548425
CGTCTCG1104.990634E-711.22420417
CGCGGTT2200.011.22400418
TCTACGG600.005877900411.0832712
TGCACGG3450.011.01089212