##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364321_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2802213
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.698223868064275	31.0	30.0	34.0	26.0	34.0
2	29.715275748131923	31.0	30.0	34.0	26.0	34.0
3	29.75330283600854	31.0	30.0	34.0	26.0	34.0
4	33.11087843786322	35.0	33.0	37.0	27.0	37.0
5	32.70995316915595	35.0	33.0	37.0	26.0	37.0
6	32.609621752522024	35.0	33.0	37.0	25.0	37.0
7	32.336079377263616	35.0	32.0	37.0	23.0	37.0
8	32.22007320642649	35.0	32.0	37.0	22.0	37.0
9	32.905809801039396	37.0	32.0	39.0	17.0	39.0
10	33.07144246351009	37.0	32.0	39.0	17.0	39.0
11	33.03611752568416	37.0	32.0	39.0	17.0	39.0
12	32.92670328772295	37.0	32.0	39.0	17.0	39.0
13	33.02776484157343	37.0	32.0	39.0	17.0	39.0
14	33.95504695752964	38.0	33.0	41.0	17.0	41.0
15	33.98714480305387	38.0	33.0	41.0	17.0	41.0
16	33.94160401083001	38.0	33.0	41.0	17.0	41.0
17	33.9271707753836	38.0	33.0	41.0	17.0	41.0
18	33.88207498858937	38.0	33.0	41.0	17.0	41.0
19	33.80020862082932	39.0	33.0	41.0	17.0	41.0
20	33.65901806893338	39.0	33.0	41.0	11.0	41.0
21	33.591339773243504	39.0	33.0	41.0	10.0	41.0
22	33.57512722980016	39.0	33.0	41.0	10.0	41.0
23	33.28275223903393	38.0	32.0	41.0	2.0	41.0
24	33.194249330796765	38.0	32.0	41.0	2.0	41.0
25	33.04797779469298	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.35124281284277004
1101	2	0.4763108946194343
1101	3	-1.265816662625447
1101	4	-0.40210389253142154
1101	5	0.4129966877421225
1101	6	0.8269396884273874
1101	7	0.9009986891741164
1101	8	1.1373422806598228
1101	9	1.8237652509147466
1101	10	1.4158237460738263
1101	11	1.541187759755374
1101	12	1.5874639217903592
1101	13	1.3569265698897652
1101	14	1.5797585520219002
1101	15	1.3887141256560582
1101	16	1.3928863177737298
1101	17	1.3672534003833334
1101	18	1.3469310375941603
1101	19	1.3941281383382957
1101	20	1.6032793791263913
1101	21	1.5456329862145637
1101	22	1.667821346235975
1101	23	1.8680231662088715
1101	24	1.736071888359973
1101	25	1.9066509542260235
1102	1	0.8239066804530033
1102	2	0.797919456413247
1102	3	0.8446756778835578
1102	4	1.0075515883496564
1102	5	1.1420504429230434
1102	6	1.024681211445973
1102	7	1.1118800622613065
1102	8	1.2569028716596478
1102	9	1.9238935547382
1102	10	1.4214962037212047
1102	11	1.4406914135295636
1102	12	1.5723192901377203
1102	13	1.3765423287862717
1102	14	1.444267296737955
1102	15	1.5305363366688667
1102	16	1.4408198019945573
1102	17	1.406907029513306
1102	18	1.5934742248809286
1102	19	1.4683397841494141
1102	20	1.7141853689642872
1102	21	1.9026842291681518
1102	22	1.8842920404887877
1102	23	2.1573083408257006
1102	24	2.24261010833812
1102	25	2.0655831806847402
1103	1	-3.060903613179459
1103	2	-2.8781148556949674
1103	3	-2.623361273617597
1103	4	-3.2528377221089357
1103	5	-5.285069319531594
1103	6	-10.566912322041329
1103	7	-10.694665632228961
1103	8	-12.67039504981841
1103	9	-13.232107764958268
1103	10	-10.873251404328943
1103	11	-14.74714098501877
1103	12	-13.352596579212328
1103	13	-13.578198680781526
1103	14	-14.176214392473526
1103	15	-12.71307303317607
1103	16	-8.728886565540908
1103	17	-6.831192607570166
1103	18	-6.22273824625071
1103	19	-6.023425309878963
1103	20	-10.969318425163067
1103	21	-8.101090102735508
1103	22	-14.37601808818162
1103	23	-15.036145964684032
1103	24	-15.222068215647369
1103	25	-16.18550226966168
1104	1	0.36632404799732043
1104	2	0.5927525029376888
1104	3	0.6113304522590965
1104	4	0.777293576800723
1104	5	0.8061785112532505
1104	6	0.8856570109864066
1104	7	0.690548997285461
1104	8	1.068619389787365
1104	9	1.3408151910680601
1104	10	1.1645823273759746
1104	11	1.0393859787348703
1104	12	1.039725872573797
1104	13	1.029794508701464
1104	14	1.1729909380657517
1104	15	1.1165749809688492
1104	16	0.7257900986154695
1104	17	0.7156159448675368
1104	18	0.9397004427247637
1104	19	1.0636916803964027
1104	20	1.4039306498508495
1104	21	1.015868739476602
1104	22	0.8662398601501309
1104	23	1.4140278999569418
1104	24	1.5647180716385733
1104	25	1.5359554429214057
1105	1	0.333368765550329
1105	2	0.4312100317862786
1105	3	-0.043617983069172084
1105	4	0.3238183872426248
1105	5	0.4222485667827911
1105	6	0.5269499883437376
1105	7	0.569905129564674
1105	8	0.5331988825036476
1105	9	0.6565670141715856
1105	10	0.3161334931539699
1105	11	0.8364362778583754
1105	12	0.9932077215044544
1105	13	0.7620713642259744
1105	14	0.6455759059095385
1105	15	0.5045832428699342
1105	16	0.7534138178563268
1105	17	0.8094008246685362
1105	18	0.7894904585334217
1105	19	0.8371781240266927
1105	20	1.042616897390694
1105	21	0.9823838095832116
1105	22	0.6623216351055916
1105	23	1.2582097612702938
1105	24	1.1984952115905045
1105	25	1.355081160910082
1106	1	-0.014994277693851643
1106	2	0.214498811240734
1106	3	0.006149084197847543
1106	4	-1.362972239192608
1106	5	-0.21549708385766309
1106	6	-8.200433219300862E-4
1106	7	0.2738452636548203
1106	8	0.42487801986143836
1106	9	0.7999893563761447
1106	10	0.4409495641940566
1106	11	0.5368824209537877
1106	12	0.686289619334886
1106	13	0.5480496377549287
1106	14	-0.03057678149693288
1106	15	0.5096159633264818
1106	16	0.28005478501061276
1106	17	0.24368901739619986
1106	18	0.24315629235569247
1106	19	-1.0454040561797413
1106	20	0.18017713577317096
1106	21	-0.07664700346190045
1106	22	0.5190182314309695
1106	23	0.5103619718239045
1106	24	0.5036864251920221
1106	25	0.7201286154607303
1107	1	0.0449794377797339
1107	2	-0.03729871751842495
1107	3	-0.24304747574034735
1107	4	-0.03217980134949272
1107	5	0.017666523305770454
1107	6	-0.02143931788812381
1107	7	-0.1491082454532986
1107	8	-0.30012278401314774
1107	9	0.5765981484176947
1107	10	0.4004528609489242
1107	11	0.42478069735976476
1107	12	0.3023760623593006
1107	13	0.2358615736848222
1107	14	0.30174855828130376
1107	15	0.11747083919521373
1107	16	-0.02132377300349475
1107	17	-0.13989983942460782
1107	18	-0.07069394537391815
1107	19	0.20538080786116808
1107	20	0.1464505774079683
1107	21	-0.01058578471850069
1107	22	0.08279264423317301
1107	23	0.33509406432249733
1107	24	0.5671505858556998
1107	25	0.1483602130332926
1108	1	-0.26647738367126195
1108	2	-0.21530455124531755
1108	3	-0.04949710499932891
1108	4	-0.26389158627755194
1108	5	-0.06399203615922744
1108	6	0.09641320489075866
1108	7	-0.4410824940556566
1108	8	0.06702883776941349
1108	9	0.2992271296553142
1108	10	0.17887974321203615
1108	11	0.235622481023384
1108	12	0.2833284793356796
1108	13	-0.257964488744058
1108	14	0.0014436871517702343
1108	15	-0.3408013704126063
1108	16	-0.8774579941123406
1108	17	-0.9624338253687625
1108	18	-1.008733562409958
1108	19	-0.8607086822443009
1108	20	-0.34027861251669833
1108	21	-0.4758442010961659
1108	22	-0.3301164488373587
1108	23	-0.8152560349416405
1108	24	-0.4453000657644637
1108	25	-0.5042844710668319
1109	1	0.05569894679995713
1109	2	0.1782895953245216
1109	3	0.05823260301455235
1109	4	0.12338148416880301
1109	5	0.0776115835365232
1109	6	0.33606831731031406
1109	7	0.5113728317833903
1109	8	0.3176463729641199
1109	9	1.1499196358264498
1109	10	0.499692572000896
1109	11	0.5382156754301946
1109	12	0.5954731448501889
1109	13	0.7619788454293186
1109	14	0.411188965087419
1109	15	0.05410134130131894
1109	16	0.5576484572930767
1109	17	0.46023725028022255
1109	18	0.37124644532966045
1109	19	0.6053056349978618
1109	20	0.9296845979282082
1109	21	0.8238587619972364
1109	22	0.4397727396308184
1109	23	0.840030789673726
1109	24	0.6182598219161477
1109	25	0.6662491936431394
1110	1	0.10127086094623294
1110	2	0.1518102959375014
1110	3	0.08164324309423776
1110	4	0.20384830189416192
1110	5	0.23883053851460545
1110	6	0.3882276960255169
1110	7	0.34685714490357356
1110	8	0.2909689971553959
1110	9	-3.089288046284892
1110	10	-1.1203839375590405
1110	11	-0.15290559980777374
1110	12	0.11277056493530324
1110	13	-0.16487696185005518
1110	14	0.11509674444737072
1110	15	0.2139072223407723
1110	16	-0.21405626485694995
1110	17	0.011899404302333494
1110	18	0.06459689376735867
1110	19	-0.10895763663079805
1110	20	0.3628341106480093
1110	21	0.09526555605917508
1110	22	0.3266790340276984
1110	23	0.4636140043442296
1110	24	0.5220097298939947
1110	25	0.6004778568062505
1111	1	-0.08285044813834119
1111	2	-0.0373664653080219
1111	3	-0.052981978659985884
1111	4	-0.05055685259915066
1111	5	0.09563444791915998
1111	6	0.09302520584800789
1111	7	0.15523428489986912
1111	8	0.16527652537124027
1111	9	0.9899856882195195
1111	10	0.36779914708305483
1111	11	0.6215553877739879
1111	12	0.6204027195701016
1111	13	0.3680651045184007
1111	14	0.28391565151193987
1111	15	0.23366375524746275
1111	16	0.17813580967423803
1111	17	0.24253468254266153
1111	18	0.16683783585549605
1111	19	0.3993438620468339
1111	20	0.5559126578993414
1111	21	0.47558357610684254
1111	22	0.5921147606741215
1111	23	0.8342129312170314
1111	24	0.6634535539707898
1111	25	0.8562380248497021
1112	1	-0.1100239257294966
1112	2	-0.18394890955861243
1112	3	-0.142052150102689
1112	4	-0.025941766685747325
1112	5	-0.14126427902177596
1112	6	-0.22050568675776105
1112	7	-0.0109150317870359
1112	8	-0.453618542308881
1112	9	0.16075994567675878
1112	10	-0.1403420630537653
1112	11	0.11512692416238934
1112	12	-0.4919234437846569
1112	13	-0.17675633921813727
1112	14	-0.9370700622464767
1112	15	-0.4112137447397828
1112	16	-0.3389304378309177
1112	17	-0.47343297648168914
1112	18	-0.5576766008797165
1112	19	-0.350255296749296
1112	20	-0.29532476582979683
1112	21	-0.40496559509788455
1112	22	-0.10379853459017596
1112	23	0.08274858170210564
1112	24	-0.12220975982388183
1112	25	-0.08511222258702844
1113	1	-1.9111268920713336
1113	2	-1.8534466729370607
1113	3	-1.9204379740517474
1113	4	-1.7642745631260972
1113	5	-1.6471015709270844
1113	6	-1.6444521194364086
1113	7	-2.0300222582368157
1113	8	-2.008687379774994
1113	9	-10.491156762270652
1113	10	-4.098213157481727
1113	11	-2.750089093350379
1113	12	-12.081935012125292
1113	13	-4.522835852582229
1113	14	-3.5699377747352585
1113	15	-3.0508939965831416
1113	16	-2.8047683104241017
1113	17	-3.018899864134152
1113	18	-3.063852573312939
1113	19	-3.8192739764078
1113	20	-14.13702568172705
1113	21	-16.86198942049445
1113	22	-11.258104091902936
1113	23	-17.51649122000758
1113	24	-18.719808550565705
1113	25	-14.865979838402634
1114	1	-0.07483002301709973
1114	2	-0.01918764526809369
1114	3	-0.014950603364287218
1114	4	-0.40926389176981104
1114	5	-0.11907194546427746
1114	6	0.09399778779951617
1114	7	-0.020105796189632485
1114	8	0.0877398975028214
1114	9	0.7211724092499523
1114	10	0.14703744826656617
1114	11	0.025685094976104494
1114	12	-0.0014504556653989198
1114	13	0.08193005396724828
1114	14	-0.055877504523991206
1114	15	0.08419087305891537
1114	16	-0.048636781700004406
1114	17	-0.18919177495736506
1114	18	-0.13691892322896138
1114	19	-0.3233554984977829
1114	20	-0.47936421886723224
1114	21	-0.6954834162142305
1114	22	-0.7973649995083605
1114	23	-0.45524738332815673
1114	24	0.03134325519517489
1114	25	-0.2985172887157006
1115	1	-0.4021083587763279
1115	2	-0.39598912183978285
1115	3	-0.34848113663129254
1115	4	-0.32929442465297143
1115	5	-0.6304949555394224
1115	6	-0.7268780754659865
1115	7	-0.8872732643228929
1115	8	-0.5661399128321065
1115	9	-0.11447200174887229
1115	10	-0.6071334982624066
1115	11	-0.3413777756254248
1115	12	-0.4409197201125252
1115	13	-0.6790523989811348
1115	14	-0.7712327388531719
1115	15	-0.9296116828825305
1115	16	-0.7461529549733967
1115	17	-0.590227588000694
1115	18	-0.6588084517301596
1115	19	-0.6247230104660488
1115	20	-1.0705109398401689
1115	21	-0.4609922553773842
1115	22	-1.0126925010974688
1115	23	-0.7529561408092746
1115	24	-0.3530136657452161
1115	25	-0.3013669956890723
1116	1	-0.4237607560366037
1116	2	-0.348727100592928
1116	3	-0.310504130760453
1116	4	-0.20467445680281315
1116	5	-1.190579523613227
1116	6	-1.0740551791841852
1116	7	-2.071400326106513
1116	8	-1.1871297436959587
1116	9	-0.11578123434602361
1116	10	-1.1918228329003746
1116	11	-0.9214266993044831
1116	12	-1.7976986200286547
1116	13	-1.4161578644549948
1116	14	-0.8213164332898515
1116	15	-2.0712362984821944
1116	16	-2.218274320642948
1116	17	-2.994049750427312
1116	18	-2.128248450332343
1116	19	-1.7203640853891642
1116	20	-1.9691143435304106
1116	21	-1.2553758170212248
1116	22	-1.1386711494061075
1116	23	-1.3704316789697444
1116	24	-1.9314559707494077
1116	25	-1.5116247186412686
1201	1	0.7444606809480838
1201	2	0.7734214054526021
1201	3	0.4094773600690935
1201	4	0.7845036879533183
1201	5	0.89904644691633
1201	6	1.0079757816100283
1201	7	1.2138519514829724
1201	8	1.1451055391097427
1201	9	1.9006400055280182
1201	10	1.5733311297447585
1201	11	1.529345637898949
1201	12	1.6198685057615876
1201	13	1.500004247032706
1201	14	1.5685003980363206
1201	15	1.6327859605981203
1201	16	1.5331090595642536
1201	17	1.4769413315178355
1201	18	1.4801592802510584
1201	19	1.634444452867129
1201	20	1.8267778225232902
1201	21	1.9084875516478021
1201	22	1.8138186945880221
1201	23	2.1267145434768295
1201	24	2.0697911146571926
1201	25	2.1150395442370282
1202	1	0.8492261375021428
1202	2	0.8796120437564667
1202	3	0.8615961878678036
1202	4	0.9362868713936763
1202	5	1.005552498291884
1202	6	1.0460745714974138
1202	7	1.2122857194775065
1202	8	1.222076782786786
1202	9	1.789300112057795
1202	10	1.5144580855373633
1202	11	1.672803172915735
1202	12	1.6444194835935733
1202	13	1.6070839786147246
1202	14	1.6658786995344883
1202	15	1.5020647653326193
1202	16	1.430376319194501
1202	17	1.562554395024847
1202	18	1.6116052809969332
1202	19	1.5161312384244283
1202	20	1.8411864528055943
1202	21	1.852492658490391
1202	22	1.8448996925890029
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2211	5	-0.6061074950091694
2211	6	0.1110915664875094
2211	7	0.33050858003533534
2211	8	0.2389935870362052
2211	9	0.9455338114389775
2211	10	0.36860397943181766
2211	11	0.6473150838637451
2211	12	0.6181579805506559
2211	13	0.4495382571563198
2211	14	0.45106209427152777
2211	15	0.45219684924222037
2211	16	0.4620908130968715
2211	17	0.39430009372292574
2211	18	0.35832893888647277
2211	19	0.03246828022071924
2211	20	0.5396221050347592
2211	21	0.6393141808184808
2211	22	0.6485598829938866
2211	23	0.8465216895455185
2211	24	0.988341931561223
2211	25	1.0741131980419283
2212	1	-0.08068711676232354
2212	2	-0.12223133686587673
2212	3	-0.04316917116295116
2212	4	0.13041582263873863
2212	5	-0.080007572314031
2212	6	0.08990731509847905
2212	7	-0.01996522058084338
2212	8	-0.06411970296675662
2212	9	0.5325758758406351
2212	10	0.15262449138686662
2212	11	0.30794058229331966
2212	12	0.061564116015603076
2212	13	-0.12698965501540727
2212	14	-0.15210705903854915
2212	15	0.05309863933498349
2212	16	0.17880058189010128
2212	17	-0.02050195350705053
2212	18	-0.006644329495102852
2212	19	0.2386817186314829
2212	20	0.5321299663600314
2212	21	0.5582421125412083
2212	22	0.25505372154547246
2212	23	0.5026007954928247
2212	24	0.6425696864718589
2212	25	0.6632490643831446
2213	1	-0.27610738838323456
2213	2	-0.1326579702380819
2213	3	-0.257415327671648
2213	4	-0.3631594724306879
2213	5	-0.2291705636328345
2213	6	-0.0026823778113822527
2213	7	-0.2322289097922372
2213	8	-0.19470221412557365
2213	9	0.25491068206017786
2213	10	-0.3004586115361505
2213	11	-0.017574275452062693
2213	12	-0.014878861494608486
2213	13	-0.30427332685617614
2213	14	-0.26511186137099685
2213	15	-0.30939220949525037
2213	16	-0.36724778390212975
2213	17	-0.4490476054251644
2213	18	-0.4627039262163919
2213	19	-0.45167927813292863
2213	20	-0.05108590693054538
2213	21	-0.27992322156863025
2213	22	-0.05349556565909808
2213	23	-0.346673635211701
2213	24	-0.25023638381553326
2213	25	0.052224028064621564
2214	1	-0.8706106503283557
2214	2	-0.7159402860785384
2214	3	-0.6898306182264733
2214	4	-0.5585338864097551
2214	5	-0.859638935961673
2214	6	-0.6796818780666989
2214	7	-0.6273022978888818
2214	8	-0.7477312020382811
2214	9	-10.567613654417645
2214	10	-3.6927077619248365
2214	11	-1.5457154338257766
2214	12	-1.0446775215258306
2214	13	-0.842493455773976
2214	14	-1.012523664596749
2214	15	-0.9841754191621774
2214	16	-0.9868811291168669
2214	17	-0.9491388473768723
2214	18	-1.2334782039674081
2214	19	-1.4006708550314855
2214	20	-1.0869263985849926
2214	21	-1.576855732454625
2214	22	-1.419690694644924
2214	23	-1.8138687070579351
2214	24	-2.2261334527358585
2214	25	-9.576369077272673
2215	1	-0.37274559756163583
2215	2	-0.08632799303993721
2215	3	0.016206939519751273
2215	4	-0.23089008774972797
2215	5	-0.166741345255069
2215	6	-0.10217185350664693
2215	7	-0.2112113373277218
2215	8	-0.31817640030072525
2215	9	0.6216267320559723
2215	10	0.4880531432135413
2215	11	0.6424042607322633
2215	12	0.5296909724642873
2215	13	0.5030323711247675
2215	14	0.43598588313511044
2215	15	-0.02811207773279989
2215	16	0.17013043032496
2215	17	0.2281826795221633
2215	18	0.29420249789260566
2215	19	0.19778736811828423
2215	20	0.4366530952779115
2215	21	0.4337652027307328
2215	22	0.21888315205723785
2215	23	0.5859119131294719
2215	24	0.782653878978266
2215	25	0.8027292415923384
2216	1	-0.5516527815878085
2216	2	-0.5826699173652159
2216	3	-0.6009503937494713
2216	4	-0.45702835529606745
2216	5	-1.3068879295224676
2216	6	-1.6461705128511355
2216	7	-1.0778736626078143
2216	8	-1.9071723010032429
2216	9	-0.6076975327013443
2216	10	-1.1736287850360192
2216	11	-0.6378442319058166
2216	12	-1.73905797275388
2216	13	-1.4456055866718458
2216	14	-1.5038915288545525
2216	15	-1.040920903418339
2216	16	-2.1302971804771786
2216	17	-2.5064603359267075
2216	18	-2.1602367008148953
2216	19	-3.55594304559472
2216	20	-2.332292726773261
2216	21	-2.3036675953026986
2216	22	-2.3651028958116598
2216	23	-1.909933607084902
2216	24	-2.130918705771297
2216	25	-2.1918241324269765
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97180.0
3	6.0
4	1380.0
5	13739.0
6	18530.0
7	14763.0
8	11828.0
9	9479.0
10	8235.0
11	7576.0
12	6684.0
13	6231.0
14	5967.0
15	6261.0
16	6461.0
17	7158.0
18	7811.0
19	9875.0
20	13451.0
21	17489.0
22	21774.0
23	25900.0
24	30264.0
25	34438.0
26	38463.0
27	42290.0
28	46898.0
29	54998.0
30	67059.0
31	82990.0
32	102717.0
33	125778.0
34	157483.0
35	196930.0
36	257390.0
37	376530.0
38	870207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.515643377608846	19.071467046086113	13.172713069160402	24.240176507144636
2	16.165903215630074	21.37511830041382	38.46776162056222	23.99121686339389
3	18.460523329426895	26.899499395477573	29.357735960020154	25.282241315075378
4	12.152433048953615	17.19739540248426	36.13786266265352	34.51230888590861
5	12.842907145062748	39.16371119888766	33.37419524114654	14.619186414903055
6	31.00792098006871	37.287173180162505	17.724522346414574	13.980383493354214
7	27.975846084822102	32.772150041813916	21.509234218236863	17.742769655127123
8	27.06633474426531	34.6044452567199	19.676539858887764	18.652680140127035
9	27.028001485035414	15.110984978295635	19.61085646561572	38.25015707105323
10	15.32078791052774	30.965903310068143	31.46344345474284	22.24986532466128
11	34.53037106003741	23.361763455339744	22.67375712076917	19.434108363853678
12	23.119302071195445	27.206161302353138	29.670575177417604	20.003961449033817
13	28.65481382086548	21.924636170926508	26.46209682599727	22.95845318221074
14	22.190840313072016	21.193102594217997	28.51626975927173	28.099787333438258
15	23.758416614710605	29.341085285150463	24.380675984378563	22.51982211576037
16	22.538322736110594	28.6990112748359	25.875948253956473	22.886717735097033
17	21.767829330653303	28.156402170483762	27.58665732081446	22.489111178048475
18	21.159223111081925	26.46102099975659	30.35053368462604	22.029222204535444
19	22.906333787337566	25.85222162259399	29.88393624524224	21.357508344826208
20	22.7025693903868	25.743586339176378	29.778502551758816	21.775341718678003
21	23.485561205697856	24.7660155837333	29.636039310524016	22.112383900044826
22	22.99922338745828	25.46089955715954	29.65949680075606	21.88038025462613
23	22.17044917991715	25.32369155591435	30.18612242159364	22.31973684257486
24	22.63638446532921	25.656967311696256	29.757334115619965	21.949314107354564
25	22.286205657264567	25.47787516406696	30.18203305132036	22.053886127348115
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1947.0
1	1947.0
2	2700.0
3	3453.0
4	3453.0
5	3453.0
6	9819.5
7	16186.0
8	16186.0
9	16186.0
10	16415.0
11	16644.0
12	16644.0
13	16644.0
14	16586.0
15	16528.0
16	16528.0
17	16528.0
18	28305.0
19	40082.0
20	40082.0
21	40082.0
22	58363.0
23	76644.0
24	76644.0
25	76644.0
26	108165.5
27	139687.0
28	139687.0
29	139687.0
30	164223.0
31	188759.0
32	188759.0
33	188759.0
34	213727.5
35	238696.0
36	238696.0
37	238696.0
38	255324.0
39	271952.0
40	271952.0
41	271952.0
42	293141.5
43	314331.0
44	314331.0
45	314331.0
46	332758.0
47	351185.0
48	351185.0
49	351185.0
50	341686.0
51	332187.0
52	332187.0
53	332187.0
54	307958.5
55	283730.0
56	283730.0
57	283730.0
58	247536.0
59	211342.0
60	211342.0
61	211342.0
62	176573.0
63	141804.0
64	141804.0
65	141804.0
66	110072.5
67	78341.0
68	78341.0
69	78341.0
70	60407.0
71	42473.0
72	42473.0
73	42473.0
74	30446.0
75	18419.0
76	18419.0
77	18419.0
78	14597.5
79	10776.0
80	10776.0
81	10776.0
82	7551.0
83	4326.0
84	4326.0
85	4326.0
86	3012.5
87	1699.0
88	1699.0
89	1699.0
90	1316.0
91	933.0
92	933.0
93	933.0
94	509.0
95	85.0
96	85.0
97	85.0
98	44.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02640769991431772
2	8.921520241323553E-4
3	3.568608096529422E-5
4	0.0032474333678417736
5	0.03240296151648715
6	0.01024190523703944
7	0.018342645616161227
8	0.026942991128797132
9	0.0332951135406195
10	0.03682803555618363
11	0.03839822311865658
12	0.007422704840781197
13	0.0026050839104664776
14	0.006209378087961194
15	0.00592388944023884
16	0.007886623893330022
17	0.009171322808080614
18	0.013239536038124153
19	0.01702226062044534
20	0.014988154005423571
21	0.011312487665998266
22	0.01074151037055356
23	0.008564659431670611
24	0.006102319845065311
25	0.10887823302511265
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2802213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.41428410364283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.93845617415336	25.83173096999436
2	13.332824636470148	9.710105284329448
3	5.556802722320812	6.070409791154578
4	2.828686796917369	4.120184186526899
5	1.6889288190031053	3.075056692300451
6	1.1205310536250654	2.448200168019442
7	0.7574562991949646	1.930756021248553
8	0.5502658351406478	1.6030029162671866
9	0.3946083105810621	1.2932441218041588
>10	2.3965870609597912	16.409753329096564
>50	0.23752900100815177	6.045587415352081
>100	0.1805416930120333	12.486469220726415
>500	0.012611023058647669	3.0459032968856863
>1k	0.003574778189852733	2.215761221170936
>5k	1.9859878832515182E-4	0.6753808559194285
>10k+	3.9719757665030364E-4	3.038454509203854
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	29198	1.0419621920246604	No Hit
TATCAACGCAGAGTACTTTTTTTTT	22782	0.8130002965513328	No Hit
GGTATCAACGCAGAGTACTTTTTTT	18845	0.6725041957909695	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	13205	0.4712346991467101	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	9727	0.34711850954941686	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	8951	0.3194261107203485	No Hit
GTGGTATCAACGCAGAGTACATGGG	3684	0.1314675222761439	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	3498	0.12482991121659916	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	3358	0.11983385988145798	No Hit
GTGGTATCAACGCAGAGTACTTTTT	3223	0.11501623895114325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.568608096529421E-5	0.0
2	0.0	0.0	0.0	3.568608096529421E-5	0.0
3	0.0	0.0	0.0	3.568608096529421E-5	0.0
4	0.0	0.0	0.0	7.137216193058843E-5	0.0
5	3.568608096529421E-5	0.0	0.0	7.137216193058843E-5	0.0
6	3.568608096529421E-5	0.0	0.0	7.137216193058843E-5	0.0
7	3.568608096529421E-5	0.0	0.0	7.137216193058843E-5	0.0
8	7.137216193058843E-5	0.0	0.0	7.137216193058843E-5	0.0
9	7.137216193058843E-5	0.0	0.0	1.0705824289588265E-4	0.0
10	7.137216193058843E-5	0.0	0.0	1.4274432386117685E-4	0.0
11	7.137216193058843E-5	0.0	0.0	1.4274432386117685E-4	0.0
12	7.137216193058843E-5	0.0	0.0	1.4274432386117685E-4	0.0
13	7.137216193058843E-5	0.0	0.0	1.4274432386117685E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCG	55	1.9590512E-4	13.819831	9
TATCACG	165	0.0	13.818104	2
TATAACG	330	0.0	13.530227	2
GGTATCA	5120	0.0	13.472071	1
TAACGCA	345	0.0	12.943805	4
ATAACGC	375	0.0	12.666822	3
GTAGGAC	105	1.9956133E-8	12.6668215	3
CGAATCG	55	0.003074971	12.088036	16
CGCGCCA	175	0.0	11.943855	10
TAGTCGG	65	8.0021186E-4	11.696627	5
GTATAAC	440	0.0	11.6602745	1
ACAACCG	165	7.2759576E-12	11.519405	5
ATGCGTA	190	0.0	11.497267	13
GTGCACG	365	0.0	11.451377	11
GTGGTAT	1420	0.0	11.374393	1
TAGACAG	385	0.0	11.354842	5
CGTCTCG	110	4.990634E-7	11.224204	17
CGCGGTT	220	0.0	11.224004	18
TCTACGG	60	0.0058779004	11.083271	2
TGCACGG	345	0.0	11.010892	12
>>END_MODULE