FastQCFastQC Report
Thu 26 May 2016
SRR1364320_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364320_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381860
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT104580.756806044027615No Hit
GTACTTTTTTTTTTTTTTTTTTTTT79230.5733576483869568No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA73840.5343522498661225No Hit
TATCAACGCAGAGTACTTTTTTTTT65340.47284095349745997No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA47520.34388432981633454No Hit
GGTATCAACGCAGAGTACTTTTTTT44490.3219573618166819No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN34970.2530647098837798No Hit
GAACAAAAAAAAAAAAAAAAAAAAA33300.24097954930311322No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30020.21724342552791165No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19980.1445877295818679No Hit
GTGGTATCAACGCAGAGTACATGGG14890.10775331799169234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC2600.017.1601813
TATAACG2450.017.048412
TAACGCA2600.016.8146064
TATCACG951.8189894E-1115.9879852
CGCATAT851.020453E-915.6461018
CTCAACG755.8977093E-815.1885853
CGTCTTA801.2730743E-714.263638515
TTGTCGA500.001500086713.2991867
TCACGCA1153.7471182E-1013.2228294
GGTATCA23750.012.9497971
GTATAAC3600.012.9203751
ATCACGC1155.3805707E-912.3819983
CTGGACG550.003058701812.0958834
TGTCGAT550.003069544412.090178
AGCACCG1650.012.08972910
CCTATAC951.0489493E-611.9909893
GTCTAAT802.8889961E-511.865651
GAACAAA10600.011.7313231
CGACAAT657.9631235E-411.70264717
TGACCGA657.9655537E-411.70222219
Produced by FastQC (version 0.11.5)