##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364320_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1381860
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.437872143343032	31.0	26.0	31.0	7.0	34.0
2	26.243587628269143	31.0	26.0	31.0	7.0	34.0
3	25.723049368242805	30.0	25.0	31.0	7.0	34.0
4	29.315763536103514	35.0	28.0	37.0	7.0	37.0
5	30.047404946955552	35.0	30.0	37.0	7.0	37.0
6	30.057923378634595	35.0	30.0	37.0	2.0	37.0
7	29.63227172072424	35.0	30.0	37.0	2.0	37.0
8	29.133837725963556	35.0	28.0	37.0	2.0	37.0
9	30.11012041740842	37.0	28.0	39.0	2.0	39.0
10	29.51028107044129	35.0	27.0	39.0	2.0	39.0
11	30.159940949155487	35.0	28.0	39.0	2.0	39.0
12	29.736610076274008	35.0	27.0	39.0	2.0	39.0
13	29.34166413384858	35.0	27.0	39.0	2.0	39.0
14	29.662931121821313	36.0	25.0	40.0	2.0	41.0
15	30.405401415483478	36.0	27.0	40.0	2.0	41.0
16	30.76994558059427	37.0	29.0	40.0	2.0	41.0
17	30.96993255467269	37.0	30.0	40.0	2.0	41.0
18	30.821171464547785	37.0	29.0	40.0	2.0	41.0
19	30.83946058211396	37.0	29.0	40.0	2.0	41.0
20	30.716247666189048	37.0	28.0	40.0	2.0	41.0
21	30.6432091528809	37.0	28.0	40.0	2.0	41.0
22	30.723979274311436	37.0	29.0	40.0	2.0	41.0
23	30.745532108896704	38.0	30.0	40.0	2.0	41.0
24	30.64297902826625	38.0	29.0	40.0	2.0	41.0
25	30.475451927112733	37.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7529998209919064
1101	2	0.9821247420962145
1101	3	1.5466572882367302
1101	4	0.9825940710738053
1101	5	0.6580542369838653
1101	6	0.4211570049705671
1101	7	0.6851476095025539
1101	8	0.7858088919426081
1101	9	0.8680101764895802
1101	10	1.1520832698363286
1101	11	0.722478157056095
1101	12	1.1163911220857408
1101	13	1.0736357935183953
1101	14	1.7026162272648122
1101	15	0.9314420581656009
1101	16	0.2958785272971838
1101	17	-0.017412102908142657
1101	18	-5.8849829904054225
1101	19	-7.00856600231964
1101	20	-8.016666394257236
1101	21	-9.858520005845055
1101	22	-9.954109264978872
1101	23	-11.418145151883774
1101	24	-10.79944431885066
1101	25	-11.254109965952441
1102	1	1.756650228776266
1102	2	1.9255263284168542
1102	3	2.6735422074275377
1102	4	2.180200430471899
1102	5	1.3841430845260234
1102	6	1.5069408159601245
1102	7	1.619511729242813
1102	8	2.019294717085625
1102	9	2.2536117648393272
1102	10	-1.2092782080947053
1102	11	0.8305843516995175
1102	12	1.8096430617577397
1102	13	2.2399226642691232
1102	14	2.78346335301255
1102	15	2.2152698099105237
1102	16	2.0036174094444377
1102	17	1.7998191628091647
1102	18	1.786978984127515
1102	19	1.655624271464255
1102	20	1.8382392147682332
1102	21	1.9432296138076381
1102	22	1.6810261801256203
1102	23	1.7245061500310435
1102	24	1.8371642138975837
1102	25	1.9675785863987443
1103	1	-0.12417985701395295
1103	2	0.30531215122723054
1103	3	-2.8421033766023527
1103	4	-0.1785348867315868
1103	5	-2.7342281406354374
1103	6	-6.694305873381968
1103	7	-7.690292408333672
1103	8	-2.507766612134919
1103	9	-7.821097792347267
1103	10	-4.975932481310622
1103	11	-8.567346401041963
1103	12	-2.55517422664704
1103	13	-0.3453695228077187
1103	14	0.06213238820535949
1103	15	-0.07002283618301419
1103	16	-0.2693414580695723
1103	17	-0.30534156188639017
1103	18	0.06340342768453056
1103	19	-5.258580918178492
1103	20	-0.8075146512998757
1103	21	-4.856161730055536
1103	22	-1.4192092954386268
1103	23	-0.3052886236048771
1103	24	-4.325644417452338
1103	25	-1.2527172957624906
1104	1	1.6394669017957497
1104	2	1.6095172738476506
1104	3	2.41833682873807
1104	4	1.7768108311582615
1104	5	1.4198981794209509
1104	6	1.1534947636936579
1104	7	1.4280763029645875
1104	8	1.6276996842655755
1104	9	1.922792362021056
1104	10	2.122614586961742
1104	11	1.7900542336136205
1104	12	1.9277614638055987
1104	13	2.3085284059402404
1104	14	2.8577781305981134
1104	15	1.8725496062124591
1104	16	1.1040813562558576
1104	17	1.106089498726547
1104	18	1.0033909644767043
1104	19	1.4684581926986837
1104	20	-9.314079010760569
1104	21	-3.4193489837029887
1104	22	-0.8210872467534642
1104	23	-0.015629456038258382
1104	24	0.4452700250459891
1104	25	0.930662774600183
1105	1	1.1732901989804105
1105	2	1.1993122253919495
1105	3	1.9804714370587888
1105	4	1.3405022697930562
1105	5	1.0697915871800419
1105	6	1.038506452794472
1105	7	1.3317206075746597
1105	8	1.5400001027388157
1105	9	1.6184208786209524
1105	10	1.9719204500499288
1105	11	1.6476849335521742
1105	12	1.8098598891240876
1105	13	1.789554643766241
1105	14	2.525012945924054
1105	15	1.9753845135407246
1105	16	1.3934964549352102
1105	17	1.019727040479463
1105	18	1.413581423012154
1105	19	1.2786265947076245
1105	20	1.1957022434233053
1105	21	1.2527816080985055
1105	22	1.2387198401715622
1105	23	1.1904329991191922
1105	24	1.3459727442638325
1105	25	1.3809785176390754
1106	1	0.7194020314638045
1106	2	0.9329792515812549
1106	3	1.7827955065758054
1106	4	1.1550029870930736
1106	5	0.29092418820415844
1106	6	0.15136601906356972
1106	7	0.6070202679479344
1106	8	0.7055214454686052
1106	9	0.9328722924524513
1106	10	-2.5531196035643227
1106	11	-0.49115276117471396
1106	12	0.2583648159804284
1106	13	1.1645699147520645
1106	14	0.43547261132366444
1106	15	0.8457818384130569
1106	16	0.4212625976761686
1106	17	-6.651608621024735
1106	18	-2.411759512654239
1106	19	-0.7434556871092539
1106	20	0.16908622407191132
1106	21	0.33994185047959036
1106	22	0.38968836972397725
1106	23	-0.2671856520775293
1106	24	0.18551918725855288
1106	25	0.04502599207980751
1107	1	0.9475794868171086
1107	2	1.200436857424787
1107	3	1.770194615189446
1107	4	1.4270056488717913
1107	5	0.8255857743353943
1107	6	0.9594608870579151
1107	7	1.038703000432296
1107	8	1.3201975957249807
1107	9	1.3377016669344535
1107	10	-1.6950329891473856
1107	11	0.4908462724689322
1107	12	0.9637219895428437
1107	13	1.5917247862541828
1107	14	1.8774615687248861
1107	15	1.6621891765311716
1107	16	-4.782483408796011
1107	17	-1.269939128654137
1107	18	0.3859147839862125
1107	19	1.1668906880227183
1107	20	1.5056757083264962
1107	21	1.5265578149616985
1107	22	1.127156688023188
1107	23	0.7974630722289149
1107	24	1.1404069386057785
1107	25	1.311120038155405
1108	1	-0.6154680919827022
1108	2	-3.218965555873087
1108	3	-0.20409795594855495
1108	4	-0.9594501290938098
1108	5	-1.0799008363966323
1108	6	-0.7216257841439919
1108	7	-0.24967991132978185
1108	8	-0.14060950536278938
1108	9	-0.7220003127669052
1108	10	-3.451488835828755
1108	11	-1.5972567943159248
1108	12	-5.670777965791903
1108	13	-1.700417737126358
1108	14	-5.6777730269171585
1108	15	-2.533345844461497
1108	16	-7.7781500204900595
1108	17	-3.5061684881544366
1108	18	-2.1677118626154517
1108	19	-0.8929126474408058
1108	20	-0.7464493268903389
1108	21	-0.23164341230197394
1108	22	-0.404588280264484
1108	23	-0.5432073875483105
1108	24	-0.4617553749167982
1108	25	-0.5238375642139168
1109	1	-8.966504815316235
1109	2	-6.22079771782419
1109	3	-5.262199015198469
1109	4	-4.912357059271422
1109	5	-2.4688798316109875
1109	6	-1.473192633025672
1109	7	-1.0978432440624069
1109	8	-0.7510373101724639
1109	9	-5.344750157583789
1109	10	-1.45319835305909
1109	11	-1.165074318995046
1109	12	-5.768670979548137
1109	13	-7.659677163249185
1109	14	-7.766059705197531
1109	15	-3.5053603215444546
1109	16	-2.043140208912398
1109	17	-1.7750707928740042
1109	18	-1.1589740519989213
1109	19	-0.9094246315820911
1109	20	-0.6532818893139982
1109	21	-0.6214834235152047
1109	22	-0.7736487716443108
1109	23	-1.0630958559410146
1109	24	-0.7176473274207531
1109	25	-0.7240212809997253
1110	1	-2.6817772167499285
1110	2	-2.447299509938887
1110	3	-1.8194499112558198
1110	4	-2.6840882420671193
1110	5	-5.476930010822457
1110	6	-4.059163959190549
1110	7	-8.584972276320471
1110	8	-9.308008636337338
1110	9	-10.785194602028234
1110	10	-8.946563944456937
1110	11	-11.813995465948452
1110	12	-6.581871696394014
1110	13	-4.783723758231261
1110	14	-4.316565081541615
1110	15	-9.787127346634062
1110	16	-11.69188931285505
1110	17	-11.883572584988698
1110	18	-8.69880559321756
1110	19	-11.99356441652833
1110	20	-12.83165106494124
1110	21	-13.871074821364665
1110	22	-14.390100384547537
1110	23	-14.942973992141729
1110	24	-15.6433605890695
1110	25	-15.95221124515743
1111	1	-0.26826754513499296
1111	2	0.4748003551083464
1111	3	-2.3924701541707982
1111	4	-2.757072497577692
1111	5	-0.47852605220797173
1111	6	-0.06868622639702693
1111	7	0.2942036121185758
1111	8	0.568617544499368
1111	9	-3.8499233469675076
1111	10	-3.7021698823786657
1111	11	-0.6787781919943363
1111	12	-5.200586946056632
1111	13	-0.4963607352892936
1111	14	0.7707457596639635
1111	15	0.9420026578643643
1111	16	0.6506711909686125
1111	17	0.6273423947683838
1111	18	0.9111161341778526
1111	19	1.2087297805027113
1111	20	1.162581953502304
1111	21	1.1442792047262031
1111	22	0.7526706455919125
1111	23	0.7524070245024461
1111	24	0.7910919525409383
1111	25	1.0718463346896243
1112	1	-0.5473061534928618
1112	2	-0.04802748960091563
1112	3	-3.0844799695779344
1112	4	-1.2425204239124916
1112	5	-0.9204191688903478
1112	6	-0.5129387460905015
1112	7	-0.18614589677807558
1112	8	-3.65419377167456
1112	9	-0.9514497239065065
1112	10	-3.505757603795953
1112	11	-1.1546008196197555
1112	12	-0.03488141674693779
1112	13	0.49600586641357225
1112	14	1.1023893075715883
1112	15	0.48544092015224294
1112	16	0.196031727970432
1112	17	-0.07990636695370767
1112	18	-0.11324196229188388
1112	19	0.046408665571266994
1112	20	0.1672153923639783
1112	21	-0.06752943479370899
1112	22	-0.07662348907871674
1112	23	-0.09766099677759144
1112	24	0.05907278912700065
1112	25	-0.0030382228753893514
1113	1	-1.0077631520523198
1113	2	-0.504689102062656
1113	3	-2.7392361278521626
1113	4	-1.1593456260333639
1113	5	-1.315517915182511
1113	6	-1.3046998241694503
1113	7	-1.207678324140442
1113	8	-8.074281491470952
1113	9	-9.277239609060583
1113	10	-3.3637003720237466
1113	11	-8.672622751461812
1113	12	-7.4595482083490054
1113	13	-2.9144466836883645
1113	14	-1.1080643783067359
1113	15	-7.262806671528576
1113	16	-2.9265109724893676
1113	17	-2.0107905878016403
1113	18	-1.3699842148771957
1113	19	-1.0316797470487238
1113	20	-6.431847970707764
1113	21	-2.438551614613612
1113	22	-1.6676531271635717
1113	23	-1.7525042312415877
1113	24	-7.59847170018061
1113	25	-4.715417996212281
1114	1	-4.205923284165657
1114	2	-4.080650321424528
1114	3	-0.883292607885032
1114	4	-0.7919883669110632
1114	5	-0.668972457763779
1114	6	-0.7096208880294981
1114	7	-0.5081670328747769
1114	8	-0.15990626430238208
1114	9	-0.5496502824277343
1114	10	-3.1849147559425823
1114	11	-1.272258886672546
1114	12	-4.338548350576286
1114	13	-1.2722431090427477
1114	14	-5.751672447334375
1114	15	-2.246525598818831
1114	16	-1.221672084390633
1114	17	-1.1118272666366416
1114	18	-0.7310814983361631
1114	19	-0.576242319150051
1114	20	-0.41010300002238864
1114	21	-0.22615097617115865
1114	22	-0.3773850287422924
1114	23	-0.6368141918920429
1114	24	-0.4602070310170667
1114	25	-0.4921488231848947
1115	1	0.2703154748721772
1115	2	0.3463059564901094
1115	3	1.0909458106693144
1115	4	-0.13083452846046484
1115	5	-0.13290624373986404
1115	6	-0.7876416775506208
1115	7	-2.572305609653803
1115	8	-0.3474976440616402
1115	9	-0.316972379806014
1115	10	0.46953706564407227
1115	11	-0.39474531740418684
1115	12	0.08851808376047288
1115	13	0.4164948989285726
1115	14	1.022223985600391
1115	15	0.2446468660813288
1115	16	-0.07008182622403325
1115	17	-0.6766242783933336
1115	18	-0.20253346012189866
1115	19	-0.18759293702743562
1115	20	0.09112374195121475
1115	21	-0.04664107210738777
1115	22	-0.3430237357061827
1115	23	-0.4939413124031695
1115	24	-0.6165941006559521
1115	25	-0.29054754937608607
1116	1	0.4271675956121044
1116	2	0.5332185180228493
1116	3	1.4046913690973817
1116	4	0.7239676814060232
1116	5	-0.12261360169809876
1116	6	-0.8341043565415411
1116	7	-4.4496786780864745
1116	8	-0.9375744935853163
1116	9	-1.120173262808219
1116	10	-0.3367743933163929
1116	11	-2.0793346859631683
1116	12	-0.5447355668870237
1116	13	-6.537466215920741
1116	14	-1.7261044487233654
1116	15	-1.4101972509754894
1116	16	-1.0233860015967728
1116	17	-0.6522171766001783
1116	18	-0.3061286248584736
1116	19	0.006214832919795299
1116	20	0.013926565669503788
1116	21	0.09622172021002129
1116	22	-0.1119988392936726
1116	23	-0.10879003334849813
1116	24	-0.12047317185095707
1116	25	0.0681272390221146
1201	1	2.296713913035319
1201	2	2.4902877916484165
1201	3	3.102447380994416
1201	4	2.828528004431483
1201	5	2.1233538360496276
1201	6	2.151073202340733
1201	7	2.3644357535581477
1201	8	2.3978550424675156
1201	9	2.8335878442836204
1201	10	3.0451036559165416
1201	11	2.475617979429696
1201	12	2.9201749889467905
1201	13	3.11134253495268
1201	14	3.772308868038216
1201	15	3.201399264010199
1201	16	2.7469752996566186
1201	17	2.5254171442363216
1201	18	2.656200942730152
1201	19	2.6062488421084566
1201	20	2.860094966804791
1201	21	2.696865421399103
1201	22	1.8276960673665599
1201	23	1.3620344112196143
1201	24	1.9589512690070627
1201	25	1.998264945318759
1202	1	2.1690736968075726
1202	2	2.4052417664826287
1202	3	3.075476543526925
1202	4	2.6821489246251353
1202	5	1.93134246985721
1202	6	1.936105808965447
1202	7	2.1148969083870384
1202	8	2.575343633481996
1202	9	2.88182233844978
1202	10	-1.124844102090389
1202	11	1.535842499146998
1202	12	2.352568551315695
1202	13	3.0561321197563522
1202	14	3.5390445197685807
1202	15	2.9390207673942896
1202	16	2.7504985268501514
1202	17	2.377671695680732
1202	18	2.718217181745331
1202	19	2.7575026800477502
1202	20	2.7279855592462816
1202	21	2.919829319072406
1202	22	2.529104283040361
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2211	17	-0.5020051814342992
2211	18	0.05497449636487062
2211	19	0.2597082319328763
2211	20	0.47276448194504894
2211	21	0.5982452190919147
2211	22	0.38684803331115347
2211	23	0.2763677214834672
2211	24	0.393870446907961
2211	25	0.5049423453641708
2212	1	0.9260017950683839
2212	2	1.0979510477525096
2212	3	1.8021898438346007
2212	4	1.3227022083576756
2212	5	0.9312980018976553
2212	6	0.748045495458463
2212	7	1.0612199492241672
2212	8	1.2919353572076808
2212	9	1.141556738804269
2212	10	1.924254034564374
2212	11	1.1385492205577883
2212	12	1.3936177905299019
2212	13	1.5037993325656416
2212	14	-4.095725174569285
2212	15	-0.6619971553577777
2212	16	-0.0498970018768361
2212	17	0.3338792162358466
2212	18	0.5448399523707437
2212	19	0.921528848458145
2212	20	1.2326870621649348
2212	21	1.1314405329473551
2212	22	1.0281510749427092
2212	23	1.0479651127042509
2212	24	1.1555270657788554
2212	25	1.2818501720906603
2213	1	0.5645245437622215
2213	2	0.6552119600645092
2213	3	1.323861015559256
2213	4	0.5669262591211037
2213	5	0.15492197035994693
2213	6	-0.10551460665162793
2213	7	0.07497359884321142
2213	8	-5.3573214061127885
2213	9	-1.7824532469673677
2213	10	0.06275095927263052
2213	11	-0.18957313702852474
2213	12	0.3926269698846596
2213	13	-8.281098800667849
2213	14	-2.5842756397646127
2213	15	-1.205092912374397
2213	16	-0.6413856026848208
2213	17	-0.35091377515310995
2213	18	-0.08830227103898736
2213	19	0.163819549716397
2213	20	0.3459298070700143
2213	21	0.34431855367776976
2213	22	0.20649207871450415
2213	23	0.10912824366020146
2213	24	0.24812609796672902
2213	25	0.4205730799795795
2214	1	-1.0764264542226947
2214	2	-0.8666372005706933
2214	3	-7.127493484240567
2214	4	-2.7879052355311735
2214	5	-1.7424724944825876
2214	6	-1.3570811705195247
2214	7	-0.8358132349261354
2214	8	-5.934980313897253
2214	9	-1.9325497218539418
2214	10	-0.20864673312796
2214	11	-0.7285837012425702
2214	12	-0.22791091208029002
2214	13	-6.579662756052613
2214	14	-1.8506914431973875
2214	15	-1.1203561048039958
2214	16	-1.0062771124193226
2214	17	-0.8574026503481775
2214	18	-0.8281127827600443
2214	19	-0.4979217150032724
2214	20	-0.12284932202184962
2214	21	-0.14219464707272778
2214	22	-0.4634337178808714
2214	23	-0.45527028843802597
2214	24	-0.21633662392788722
2214	25	-0.05713499243411846
2215	1	0.48692995863082444
2215	2	0.7065735208875132
2215	3	-2.172577874829379
2215	4	-0.2619914189133006
2215	5	0.255653230921407
2215	6	-0.09585794568764427
2215	7	0.7903494382344043
2215	8	1.0287087970640343
2215	9	1.2589029538413428
2215	10	1.7952161361417964
2215	11	1.0875009489780147
2215	12	0.7584519546239363
2215	13	1.3385807375032854
2215	14	1.6101752753956333
2215	15	1.2679259846057995
2215	16	1.1560202490856
2215	17	1.0426155136139066
2215	18	1.002764721326848
2215	19	1.0117896421936052
2215	20	1.3368013347028693
2215	21	1.417331940555048
2215	22	1.1241310489585068
2215	23	0.9326678145259848
2215	24	0.8376511867194765
2215	25	1.0175024723283066
2216	1	0.1342957949841015
2216	2	-0.03497037767754918
2216	3	1.0215374855281887
2216	4	-0.30719182022154357
2216	5	-0.5392547971472439
2216	6	-0.8205789961310082
2216	7	-1.374674145073186
2216	8	-6.290314879999514
2216	9	-2.1628002807186597
2216	10	-0.8549562376627904
2216	11	-1.4092920036856675
2216	12	-0.16597194266375226
2216	13	0.4783489637736622
2216	14	0.9020082923537913
2216	15	0.4171050161067775
2216	16	-0.10295232893501449
2216	17	-0.16837571406301421
2216	18	0.04287425612330509
2216	19	0.25106835183325416
2216	20	0.062305185964163456
2216	21	-0.05110495319140895
2216	22	-0.14452889389130164
2216	23	-0.3838037762935471
2216	24	-0.2613718885045273
2216	25	-0.13933568380729255
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	125696.0
3	8.0
4	661.0
5	5699.0
6	11331.0
7	9288.0
8	7316.0
9	5918.0
10	5126.0
11	5084.0
12	4806.0
13	4649.0
14	4778.0
15	4505.0
16	4204.0
17	4573.0
18	5363.0
19	7422.0
20	10700.0
21	13336.0
22	16323.0
23	18410.0
24	20732.0
25	22762.0
26	24529.0
27	26737.0
28	28481.0
29	32875.0
30	37655.0
31	42495.0
32	53509.0
33	69104.0
34	87485.0
35	111453.0
36	143334.0
37	171892.0
38	233621.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.14632290810682	18.905147957687383	13.270386104085787	20.67814303012001
2	13.33765510652832	23.80065349275982	43.261772415810725	19.599918984901137
3	14.83978184542738	33.854252881574475	30.528136189787507	20.777829083210637
4	9.532732807734277	19.812407748508186	39.624016861376454	31.03084258238108
5	10.621174970612303	42.92905493956554	32.68972036852426	13.760049721297896
6	24.63360473723168	40.99214815459863	20.99737304248124	13.376874065688451
7	24.72667155809368	37.19792353843218	22.31143875447141	15.763966149002737
8	24.786529476567228	36.64305173947815	21.568977504132974	17.00144127982165
9	27.901316099816032	19.2368290231397	20.181430909295432	32.68042396774884
10	12.479857604707762	34.92462494097134	32.575465872352794	20.020051581968108
11	30.61496559737058	28.23998310530086	23.65112736556631	17.49392393176225
12	21.23528698854165	31.691204378688003	29.881991107059996	17.19151752571035
13	25.56596564838688	27.43230799038927	27.04197183977568	19.95975452144817
14	19.777613606180722	26.327213667720876	30.658828037347906	23.23634468875049
15	21.939463778916746	32.858145308119944	25.746289505976193	19.456101406987113
16	19.773003530491764	33.00077286202055	26.985438989256128	20.24078461823156
17	19.186150716969028	32.13360781892079	27.36324761826669	21.316993845843495
18	18.45121514940195	30.809448928603306	30.982227927501032	19.757107994493715
19	20.46134856068044	30.47203760103973	30.808891133611553	18.25772270466827
20	21.541857779820194	29.37323126412444	30.38110168431646	18.70380927173891
21	22.730772384596573	29.13028996623507	29.710672345193267	18.42826530397509
22	21.230623649796485	29.665182183590673	29.868297783870197	19.23589638274265
23	20.743700841967925	29.854703751085747	29.872191837780214	19.52940356916611
24	19.508343758798755	30.03201658011837	30.842735603424003	19.61690405765887
25	20.119755786430677	29.249683268293886	30.63582892279293	19.99473202248251
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12024.0
1	12024.0
2	12462.0
3	12900.0
4	12900.0
5	12900.0
6	18619.5
7	24339.0
8	24339.0
9	24339.0
10	22070.0
11	19801.0
12	19801.0
13	19801.0
14	20612.0
15	21423.0
16	21423.0
17	21423.0
18	28935.0
19	36447.0
20	36447.0
21	36447.0
22	47777.5
23	59108.0
24	59108.0
25	59108.0
26	76990.5
27	94873.0
28	94873.0
29	94873.0
30	107925.5
31	120978.0
32	120978.0
33	120978.0
34	127379.0
35	133780.0
36	133780.0
37	133780.0
38	139402.5
39	145025.0
40	145025.0
41	145025.0
42	150975.5
43	156926.0
44	156926.0
45	156926.0
46	159180.0
47	161434.0
48	161434.0
49	161434.0
50	150825.5
51	140217.0
52	140217.0
53	140217.0
54	120968.5
55	101720.0
56	101720.0
57	101720.0
58	84537.5
59	67355.0
60	67355.0
61	67355.0
62	53162.0
63	38969.0
64	38969.0
65	38969.0
66	28787.0
67	18605.0
68	18605.0
69	18605.0
70	13623.0
71	8641.0
72	8641.0
73	8641.0
74	6188.0
75	3735.0
76	3735.0
77	3735.0
78	2819.5
79	1904.0
80	1904.0
81	1904.0
82	1383.0
83	862.0
84	862.0
85	862.0
86	675.5
87	489.0
88	489.0
89	489.0
90	366.5
91	244.0
92	244.0
93	244.0
94	143.0
95	42.0
96	42.0
97	42.0
98	30.5
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3114642583184982
2	0.3139970764042667
3	0.32477964482653815
4	0.3264440681400431
5	0.331654436773624
6	0.2778863271243107
7	0.2850505839954844
8	0.28353089314402324
9	0.284037456761177
10	0.39150130982878145
11	0.2831690619889135
12	0.2831690619889135
13	0.27622190381080575
14	0.2820835685235841
15	0.27564297396263004
16	0.2796954828998596
17	0.2726035922597079
18	0.2749193116524105
19	0.2759324388867179
20	0.47645926504855773
21	0.27520877657649834
22	0.2769455661210253
23	0.2734719870319714
24	0.2767284674279594
25	0.2693471118637199
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1381860.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.808324169544115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.41625144312638	54.502678614302994
2	8.45847910630263	10.79442753592548
3	2.6281802353990633	5.030993293089966
4	1.1579452381982784	2.955461805181429
5	0.6162453169237244	1.9660790475116232
6	0.3666487942229494	1.4037147070890255
7	0.24136259275306168	1.0780659792552316
8	0.17638203377743977	0.9003713591163486
9	0.12796634407819185	0.7348786169159419
>10	0.7097793147634347	8.41835235989344
>50	0.06147132149549355	2.722452311480795
>100	0.035091334173511755	3.9964016193975582
>500	0.002215043636975655	0.9131193054864978
>1k	0.0015155561726675534	2.180328052448819
>5k	3.497437321540508E-4	1.6247200611919022
>10k+	1.1658124405135027E-4	0.7779553317130586
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10458	0.756806044027615	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7923	0.5733576483869568	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7384	0.5343522498661225	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6534	0.47284095349745997	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	4752	0.34388432981633454	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4449	0.3219573618166819	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3497	0.2530647098837798	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3330	0.24097954930311322	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3002	0.21724342552791165	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1998	0.1445877295818679	No Hit
GTGGTATCAACGCAGAGTACATGGG	1489	0.10775331799169234	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	7.236623102195591E-5	0.0
12	0.0	0.0	0.0	7.236623102195591E-5	0.0
13	0.0	0.0	0.0	7.236623102195591E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	260	0.0	17.160181	3
TATAACG	245	0.0	17.04841	2
TAACGCA	260	0.0	16.814606	4
TATCACG	95	1.8189894E-11	15.987985	2
CGCATAT	85	1.020453E-9	15.646101	8
CTCAACG	75	5.8977093E-8	15.188585	3
CGTCTTA	80	1.2730743E-7	14.2636385	15
TTGTCGA	50	0.0015000867	13.299186	7
TCACGCA	115	3.7471182E-10	13.222829	4
GGTATCA	2375	0.0	12.949797	1
GTATAAC	360	0.0	12.920375	1
ATCACGC	115	5.3805707E-9	12.381998	3
CTGGACG	55	0.0030587018	12.095883	4
TGTCGAT	55	0.0030695444	12.09017	8
AGCACCG	165	0.0	12.089729	10
CCTATAC	95	1.0489493E-6	11.990989	3
GTCTAAT	80	2.8889961E-5	11.86565	1
GAACAAA	1060	0.0	11.731323	1
CGACAAT	65	7.9631235E-4	11.702647	17
TGACCGA	65	7.9655537E-4	11.702222	19
>>END_MODULE