FastQCFastQC Report
Thu 26 May 2016
SRR1364320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381860
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT258811.8729104250792412No Hit
GTATCAACGCAGAGTACTTTTTTTT238951.7291910902696366No Hit
TATCAACGCAGAGTACTTTTTTTTT191371.3848725630671703No Hit
GGTATCAACGCAGAGTACTTTTTTT165641.1986742506476777No Hit
GAGTACTTTTTTTTTTTTTTTTTTT109430.7919036660732636No Hit
ACGCAGAGTACTTTTTTTTTTTTTT67640.4894851866325098No Hit
GTGGTATCAACGCAGAGTACTTTTT33290.24090718307209122No Hit
GTATAACGCAGAGTACTTTTTTTTT32010.2316443055012809No Hit
GCAGAGTACTTTTTTTTTTTTTTTT29130.21080283096695757No Hit
ATCAACGCAGAGTACTTTTTTTTTT27450.19864530415526896No Hit
GTGGTATCAACGCAGAGTACATGGG23070.1669488949676523No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18920.1369169090935406No Hit
GGTATCAACGCAGAGTACATGGGAT18770.13583141562821124No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT18560.13431172477675019No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT14260.10319424543730914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA5100.016.7648324
TATAACG4950.016.3114852
TATCACG1700.016.2042472
ATAACGC5100.016.2042473
GTATAAC5900.014.3321751
CCGGTCT801.2845157E-714.2532059
TCCGGTC801.2845157E-714.2532058
TTGCGCG903.6545316E-813.7173618
CGTCTTA707.279603E-613.56809315
GGTATCA41500.013.0954991
TGCTCCG801.9906602E-613.067335
CTCCGGT801.9938998E-613.0654387
CATTCCG957.3627234E-813.0029249
TACAACG1207.403287E-1012.6653882
GCGCCAC751.4820414E-512.66355313
AGCACCG1600.012.47155510
ATCACGC2150.012.3708443
TTTGCGC1001.4472607E-712.34651817
GCACCGA1551.8189894E-1212.25860211
CACCGAC1551.8189894E-1212.25416612
Produced by FastQC (version 0.11.5)