##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364320_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1381860 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.537132560461988 31.0 30.0 34.0 26.0 34.0 2 29.490535220644638 31.0 30.0 34.0 25.0 34.0 3 29.54514784420998 31.0 30.0 34.0 25.0 34.0 4 32.87124527810342 35.0 33.0 37.0 26.0 37.0 5 32.51577294371354 35.0 32.0 37.0 25.0 37.0 6 32.373514683108276 35.0 32.0 37.0 23.0 37.0 7 32.10044071034692 35.0 32.0 37.0 19.0 37.0 8 31.95534062784942 35.0 32.0 37.0 18.0 37.0 9 32.60422184591782 37.0 32.0 39.0 11.0 39.0 10 32.766396740624955 37.0 32.0 39.0 17.0 39.0 11 32.69146657403789 37.0 32.0 39.0 15.0 39.0 12 32.65376376767545 37.0 32.0 39.0 15.0 39.0 13 32.689186314098386 37.0 32.0 39.0 15.0 39.0 14 33.549842965278685 38.0 32.0 41.0 16.0 41.0 15 33.59525349890727 38.0 32.0 41.0 16.0 41.0 16 33.5619107579639 38.0 32.0 41.0 15.0 41.0 17 33.61555077938431 38.0 32.0 41.0 15.0 41.0 18 33.60675900597745 38.0 32.0 41.0 13.0 41.0 19 33.56210542312535 39.0 32.0 41.0 11.0 41.0 20 33.46254179149842 39.0 32.0 41.0 10.0 41.0 21 33.387109403268056 38.0 32.0 41.0 10.0 41.0 22 33.31281388852706 38.0 32.0 41.0 2.0 41.0 23 33.04920541878338 38.0 32.0 41.0 2.0 41.0 24 32.963371832168235 38.0 32.0 41.0 2.0 41.0 25 32.80883953511933 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24417101015905018 1101 2 0.37993236910365624 1101 3 -1.4020223056396794 1101 4 -0.35967067230573946 1101 5 0.3502186022613145 1101 6 0.6857385460610956 1101 7 0.7204910301706882 1101 8 0.8743968142886764 1101 9 1.33958783471995 1101 10 1.0674615783715922 1101 11 1.0571870765749125 1101 12 1.0024862169709152 1101 13 0.8903989965636043 1101 14 1.1046375989021442 1101 15 1.0769841640212476 1101 16 1.0692921349800741 1101 17 0.9820643928304662 1101 18 0.9925435461583305 1101 19 1.201698438971512 1101 20 1.3342148044632154 1101 21 1.318386808520593 1101 22 1.357317170558872 1101 23 1.5323233046382185 1101 24 1.6511644559297238 1101 25 1.5187386875277724 1102 1 0.499287558100896 1102 2 0.45551285915066586 1102 3 0.5344889181898012 1102 4 0.643434523222993 1102 5 0.6350988430369284 1102 6 0.7870126047649322 1102 7 0.9298966473566956 1102 8 1.0250092738002685 1102 9 1.5769891749010796 1102 10 1.336721002802129 1102 11 1.0831852318518926 1102 12 1.1932904359256895 1102 13 1.017737597635417 1102 14 1.1589188604950706 1102 15 1.344197057497091 1102 16 1.263935556901579 1102 17 1.1151694075039984 1102 18 1.2428440525087723 1102 19 1.119043864018721 1102 20 1.4207919522179395 1102 21 1.5150491920068347 1102 22 1.617267404501689 1102 23 2.010975112947591 1102 24 1.9192538354508244 1102 25 1.8433995730036443 1103 1 -1.8558123468826473 1103 2 -1.9674893854409703 1103 3 -2.0529927785195063 1103 4 -1.7735041108351304 1103 5 -4.163704959457366 1103 6 -9.04856761528767 1103 7 -9.360550756215371 1103 8 -9.69176365523116 1103 9 -10.703038549707266 1103 10 -8.391215212238013 1103 11 -9.625522773858279 1103 12 -10.788206601121548 1103 13 -11.04360539340917 1103 14 -11.920945454170052 1103 15 -11.261773831664144 1103 16 -5.350192058919809 1103 17 -3.5514743083279505 1103 18 -2.7857251465529984 1103 19 -2.529032626523236 1103 20 -7.639903825287487 1103 21 -3.8074559375047237 1103 22 -8.686551155379334 1103 23 -9.666180085186966 1103 24 -10.71898592732935 1103 25 -11.975954406900605 1104 1 0.44251207767713296 1104 2 0.6137226312694075 1104 3 0.7147544361652969 1104 4 0.882857721498425 1104 5 1.0334027898143532 1104 6 0.8761464418192304 1104 7 0.6419978487826192 1104 8 0.9105995141746881 1104 9 1.3243472319506466 1104 10 1.236517542705677 1104 11 0.973121096028251 1104 12 1.0424843506500707 1104 13 0.8516401348100118 1104 14 1.0772380030390991 1104 15 1.1201493744053153 1104 16 0.7315508541432649 1104 17 0.7531134831734718 1104 18 0.9357720719131208 1104 19 1.0453125256821778 1104 20 1.2675099683375208 1104 21 1.165075091950193 1104 22 0.8793737689187822 1104 23 1.2526895744646112 1104 24 1.3297037720713902 1104 25 1.6745463902001845 1105 1 0.4285490948795072 1105 2 0.4204662640565644 1105 3 0.09561014357045394 1105 4 0.37692048218597307 1105 5 0.5066328608415205 1105 6 0.5901157755233228 1105 7 0.7654041145526058 1105 8 0.6834078506834764 1105 9 0.635370431640311 1105 10 0.357723305209241 1105 11 0.9018810901236236 1105 12 1.1093997325455263 1105 13 0.8376986077774689 1105 14 0.5710308502019572 1105 15 0.4097459888572317 1105 16 0.7118930821785696 1105 17 0.7474002420484354 1105 18 0.7765454063449866 1105 19 0.9043906315329053 1105 20 1.0467224216068658 1105 21 1.0169127353176108 1105 22 0.7232367335219152 1105 23 1.148899456475064 1105 24 1.134728798075777 1105 25 1.2329537030115105 1106 1 -0.32086668083421443 1106 2 -0.09946106350346184 1106 3 -0.18485372789496424 1106 4 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0.21475408636555926 2210 20 0.2554792143427349 2210 21 0.39617092385515207 2210 22 0.3004007228323644 2210 23 0.6931006704748057 2210 24 0.7020687776106413 2210 25 0.9108451483864854 2211 1 -0.24293376383578646 2211 2 -0.09789748476845617 2211 3 -0.06627564126612029 2211 4 -0.007831834894922451 2211 5 -0.7014441690090116 2211 6 -0.06736962552770365 2211 7 0.2150413933766977 2211 8 -9.34624343678081E-4 2211 9 0.6079435237926702 2211 10 -0.1226910906450982 2211 11 0.5470085234847204 2211 12 0.3730246065245737 2211 13 0.3512668636474885 2211 14 0.303537072105982 2211 15 0.16941063918866206 2211 16 0.14777581329003908 2211 17 0.1537319730284068 2211 18 0.03296699574315909 2211 19 -0.10258191903376002 2211 20 0.22063255344791344 2211 21 0.5038394313039376 2211 22 0.25380685961592775 2211 23 0.7619615586263109 2211 24 0.6199953049941413 2211 25 0.6155600111211683 2212 1 -0.032599226766706124 2212 2 -0.08729235249547074 2212 3 -0.030595365775241135 2212 4 0.20634850919833525 2212 5 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-0.26364014750867426 2213 17 -0.40721920189194805 2213 18 -0.3896336823214597 2213 19 -0.42384847214097476 2213 20 -0.050998754073248165 2213 21 -0.21136245648486351 2213 22 -0.0813662807155211 2213 23 -0.4523962013424523 2213 24 -0.213358367628814 2213 25 0.15102690755558967 2214 1 -1.0529391183833283 2214 2 -0.8167225440509469 2214 3 -0.7224377622335645 2214 4 -0.5896433718200527 2214 5 -0.8500830090270277 2214 6 -0.6381791254582971 2214 7 -0.6027906664809066 2214 8 -0.5858597315054546 2214 9 -10.281791326945772 2214 10 -3.7253768636972744 2214 11 -1.739334547192474 2214 12 -1.180829392812484 2214 13 -0.912800156022687 2214 14 -1.0717985786981927 2214 15 -1.1924712151279806 2214 16 -1.404692803111871 2214 17 -1.2117819077153413 2214 18 -1.4948690021150242 2214 19 -1.7376652544919153 2214 20 -1.3924308426951093 2214 21 -1.9062458164926248 2214 22 -1.6316664512226424 2214 23 -2.155280367764245 2214 24 -2.322558049247423 2214 25 -9.932014718814148 2215 1 -0.3472304006051168 2215 2 -0.1427022435143428 2215 3 0.029495263030764107 2215 4 -0.15696732762312848 2215 5 -5.181744576248093E-4 2215 6 0.01626062919481086 2215 7 -0.1025171808371077 2215 8 -0.235907706942033 2215 9 0.7622364752045527 2215 10 0.5793756292295527 2215 11 0.6369381872637447 2215 12 0.6700309324763722 2215 13 0.4990835905151272 2215 14 0.3810712760003767 2215 15 0.006140524538317038 2215 16 0.2813742065150251 2215 17 0.2634351618936819 2215 18 0.3360528343539002 2215 19 0.3791659653566768 2215 20 0.6073333336615718 2215 21 0.4833182176309947 2215 22 0.2853004339246752 2215 23 0.7963107234793938 2215 24 1.0036728670474986 2215 25 1.0800812484784004 2216 1 -0.7911654649845481 2216 2 -0.7671072872572253 2216 3 -0.7874603582631394 2216 4 -0.5793784401194273 2216 5 -1.6938661894004383 2216 6 -1.9782796216903975 2216 7 -1.4290100141359474 2216 8 -2.2874194398448253 2216 9 -0.963751713592103 2216 10 -1.4934350822041473 2216 11 -0.9704094137608585 2216 12 -2.2617200541617706 2216 13 -1.9512176670224513 2216 14 -2.006414105337644 2216 15 -1.6914148400935396 2216 16 -2.861549583198965 2216 17 -3.0231159966167276 2216 18 -2.6987547806767758 2216 19 -3.8117336476031802 2216 20 -2.8343393053413592 2216 21 -2.9659879575906984 2216 22 -2.746228179144289 2216 23 -2.5257546997540317 2216 24 -2.527583997045056 2216 25 -2.5227894033076765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51562.0 3 5.0 4 698.0 5 7361.0 6 10932.0 7 8795.0 8 6705.0 9 5190.0 10 4431.0 11 3964.0 12 3380.0 13 3225.0 14 3122.0 15 3095.0 16 3224.0 17 3243.0 18 3948.0 19 5273.0 20 7422.0 21 9981.0 22 11770.0 23 13567.0 24 15253.0 25 17192.0 26 19172.0 27 20789.0 28 22893.0 29 26286.0 30 31860.0 31 39893.0 32 49330.0 33 60781.0 34 75672.0 35 97036.0 36 127468.0 37 194941.0 38 412401.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.20878829751889 19.572948327167413 13.60907812342791 21.609185251885787 2 14.924933530750984 23.28355919128236 41.31738650294317 20.474120775023483 3 17.717607538261916 31.683958615109958 30.263478782536836 20.334955064091293 4 10.769707289926046 19.032487076742044 38.83714921939397 31.360656413937942 5 10.727007789601847 40.886976576180075 34.49524668520265 13.890768949015422 6 25.006604111557014 39.89636068219568 21.12765657172427 13.96937863452304 7 23.770163128996256 34.94780711722445 24.354265977420482 16.927763776358816 8 25.528006016774874 34.73652360418713 22.218273130597748 17.51719724844025 9 27.07879231875696 15.804721472776354 21.819021551465152 35.297464657001534 10 14.830752346127143 32.28211586172347 32.84194309931305 20.04518869283634 11 31.314613607010266 24.6456930053689 25.324407364720464 18.715286022900365 12 21.963418667581454 29.436588424909697 31.482699749519266 17.117293157989582 13 27.581889773466578 23.866808218394922 28.48381164935987 20.06749035877863 14 21.186192901629923 23.162988083503766 32.30466166153561 23.3461573533307 15 22.59790674886052 29.63726340197456 28.517398648404154 19.24743120076076 16 19.66789435555018 29.399035556176358 30.00624579783735 20.926824290436112 17 19.068910737742588 28.441972476234245 31.157004157876262 21.332112628146906 18 18.256023507784057 26.328501016885365 35.42358160776452 19.99189386756606 19 20.189294311616912 26.259247947031756 35.41288799398983 18.1385697473615 20 20.689954656813963 25.2648758581008 35.28134126085178 18.763828224233453 21 21.769765991046086 24.925854464871154 34.822202697753994 18.482176846328766 22 20.317062844446696 25.58156533948015 35.04503363500501 19.056338181068142 23 19.943753546160885 25.31394379407371 35.40060038674865 19.341702273016757 24 19.021859090103163 25.93130000253292 35.803025752548294 19.24381515481562 25 19.566921319403498 25.057481589230118 35.66176630383602 19.71383078753037 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2701.0 1 2701.0 2 3994.5 3 5288.0 4 5288.0 5 5288.0 6 17964.5 7 30641.0 8 30641.0 9 30641.0 10 24878.0 11 19115.0 12 19115.0 13 19115.0 14 16892.0 15 14669.0 16 14669.0 17 14669.0 18 23114.0 19 31559.0 20 31559.0 21 31559.0 22 44474.5 23 57390.0 24 57390.0 25 57390.0 26 80824.5 27 104259.0 28 104259.0 29 104259.0 30 115199.5 31 126140.0 32 126140.0 33 126140.0 34 132861.0 35 139582.0 36 139582.0 37 139582.0 38 139256.0 39 138930.0 40 138930.0 41 138930.0 42 144286.5 43 149643.0 44 149643.0 45 149643.0 46 154915.0 47 160187.0 48 160187.0 49 160187.0 50 152023.0 51 143859.0 52 143859.0 53 143859.0 54 124935.0 55 106011.0 56 106011.0 57 106011.0 58 88725.5 59 71440.0 60 71440.0 61 71440.0 62 57421.0 63 43402.0 64 43402.0 65 43402.0 66 31804.0 67 20206.0 68 20206.0 69 20206.0 70 14695.0 71 9184.0 72 9184.0 73 9184.0 74 6557.0 75 3930.0 76 3930.0 77 3930.0 78 2994.0 79 2058.0 80 2058.0 81 2058.0 82 1482.0 83 906.0 84 906.0 85 906.0 86 670.0 87 434.0 88 434.0 89 434.0 90 370.5 91 307.0 92 307.0 93 307.0 94 163.0 95 19.0 96 19.0 97 19.0 98 9.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024676884778486965 2 0.0013025921583952064 3 2.1709869306586773E-4 4 0.0035459453200758395 5 0.029163591101848234 6 0.010493103498183607 7 0.0183810226795768 8 0.028150463867540852 9 0.033939762349297325 10 0.03915013098287815 11 0.04139348414455878 12 0.008466849029568842 13 0.002388085623724545 14 0.005644566019712562 15 0.006223495867888209 16 0.009479976263876226 17 0.010493103498183607 18 0.013749583894171623 19 0.014617978666435095 20 0.012664090428842286 21 0.008539215260590798 22 0.008322116567524929 23 0.006078763405844297 24 0.003980142706207575 25 0.10261531558913349 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1381860.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.02236891748048 #Duplication Level Percentage of deduplicated Percentage of total 1 77.256308351369 38.645435575548085 2 12.683259608329195 12.68893382408044 3 4.3316061411923235 6.500316011998393 4 1.924367159098933 3.850456158605227 5 1.0065901987120112 2.5176013134346102 6 0.5908687220179393 1.7733991916748948 7 0.3859963393755606 1.3515915902328903 8 0.27841218013273783 1.1141469428575872 9 0.2077979238903212 0.9355089968215359 >10 1.1660645700287 11.044536222215354 >50 0.10198554085553563 3.5475458967592384 >100 0.06050423552414519 5.47517402946907 >500 0.0034822490085337026 1.1656963017071114 >1k 0.0018862182129557553 1.900634397161081 >5k 1.4509370868890427E-4 0.49092645443913213 >10k+ 7.254685434445213E-4 6.998097092995435 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTT 25881 1.8729104250792412 No Hit GTATCAACGCAGAGTACTTTTTTTT 23895 1.7291910902696366 No Hit TATCAACGCAGAGTACTTTTTTTTT 19137 1.3848725630671703 No Hit GGTATCAACGCAGAGTACTTTTTTT 16564 1.1986742506476777 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 10943 0.7919036660732636 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6764 0.4894851866325098 No Hit GTGGTATCAACGCAGAGTACTTTTT 3329 0.24090718307209122 No Hit GTATAACGCAGAGTACTTTTTTTTT 3201 0.2316443055012809 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 2913 0.21080283096695757 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2745 0.19864530415526896 No Hit GTGGTATCAACGCAGAGTACATGGG 2307 0.1669488949676523 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1892 0.1369169090935406 No Hit GGTATCAACGCAGAGTACATGGGAT 1877 0.13583141562821124 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 1856 0.13431172477675019 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 1426 0.10319424543730914 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.236623102195591E-5 0.0 0.0 0.0 0.0 3 7.236623102195591E-5 0.0 0.0 0.0 0.0 4 7.236623102195591E-5 0.0 0.0 0.0 0.0 5 7.236623102195591E-5 0.0 0.0 0.0 0.0 6 7.236623102195591E-5 0.0 0.0 0.0 0.0 7 7.236623102195591E-5 0.0 0.0 0.0 0.0 8 7.236623102195591E-5 0.0 0.0 7.236623102195591E-5 0.0 9 7.236623102195591E-5 0.0 0.0 7.236623102195591E-5 0.0 10 7.236623102195591E-5 0.0 0.0 7.236623102195591E-5 0.0 11 1.4473246204391182E-4 0.0 0.0 1.4473246204391182E-4 0.0 12 1.4473246204391182E-4 0.0 0.0 1.4473246204391182E-4 0.0 13 2.8946492408782364E-4 0.0 0.0 1.4473246204391182E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 510 0.0 16.764832 4 TATAACG 495 0.0 16.311485 2 TATCACG 170 0.0 16.204247 2 ATAACGC 510 0.0 16.204247 3 GTATAAC 590 0.0 14.332175 1 CCGGTCT 80 1.2845157E-7 14.253205 9 TCCGGTC 80 1.2845157E-7 14.253205 8 TTGCGCG 90 3.6545316E-8 13.71736 18 CGTCTTA 70 7.279603E-6 13.568093 15 GGTATCA 4150 0.0 13.095499 1 TGCTCCG 80 1.9906602E-6 13.06733 5 CTCCGGT 80 1.9938998E-6 13.065438 7 CATTCCG 95 7.3627234E-8 13.002924 9 TACAACG 120 7.403287E-10 12.665388 2 GCGCCAC 75 1.4820414E-5 12.663553 13 AGCACCG 160 0.0 12.471555 10 ATCACGC 215 0.0 12.370844 3 TTTGCGC 100 1.4472607E-7 12.346518 17 GCACCGA 155 1.8189894E-12 12.258602 11 CACCGAC 155 1.8189894E-12 12.254166 12 >>END_MODULE