FastQCFastQC Report
Thu 26 May 2016
SRR1364319_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364319_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007901
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79840.3976291659797968No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN52400.2609690418003676No Hit
TATCAACGCAGAGTACTTTTTTTTT50640.25220366940401945No Hit
GGTATCAACGCAGAGTACTTTTTTT35310.1758552837017363No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32900.16385269990900947No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA21790.10852128665706127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG402.7521452E-416.6324865
GGTATCA19000.013.2896981
TTGACCG1650.013.25302918
TATCACG957.466042E-812.9899312
ATAACGC1800.012.6568553
TGACCGA1850.012.33419419
GTTCTAG1700.012.2845951
TATAACG1701.8189894E-1211.7262042
TAACGCA1751.8189894E-1211.4051334
ACTATCC1251.8319042E-811.4002858
CGCATAT1001.9297186E-611.4002858
GTGGTAT6100.011.204431
GTATCAA47100.011.08482551
ACCGTGC600.00587556111.0836118
GTCTTAG2000.010.9165381
CGCAGAT4450.010.6744248
TGGTATC6000.010.4419062
GGACCGA1106.0378443E-610.3649326
GTCCTAC1701.5807018E-910.0510321
CAACGGA951.6369039E-410.0045035
Produced by FastQC (version 0.11.5)