FastQCFastQC Report
Thu 26 May 2016
SRR1364319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364319_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007901
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT174760.8703616363555773No Hit
TATCAACGCAGAGTACTTTTTTTTT132300.6588970272936763No Hit
GGTATCAACGCAGAGTACTTTTTTT121810.6066534156813509No Hit
GTACTTTTTTTTTTTTTTTTTTTTT80360.40021893509689965No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54380.2708300857462594No Hit
GAGTACTTTTTTTTTTTTTTTTTTT48570.24189439618785988No Hit
GTGGTATCAACGCAGAGTACATGGG28200.14044517135057955No Hit
GTGGTATCAACGCAGAGTACTTTTT23270.11589216799035411No Hit
ATCAACGCAGAGTACTTTTTTTTTT20740.10329194517060354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA2350.016.5752744
ATAACGC2550.014.9008263
TATAACG2600.014.2482072
GGTATCA31700.013.5487621
GGACCGA1550.013.484866
ACGAAAT500.001502364313.29700112
ATCACGC1650.013.2414183
TATCACG1450.013.1017992
ACCGAGT1850.012.838788
TACGCAG751.4757048E-512.6694915
GTGGTAT9350.012.5001131
TGGTATC8950.012.3112992
AGGACCG2350.012.1303635
CCGAGTG1900.012.0005839
CGAGTGG1850.011.81138310
GTATAAC3800.011.5025631
CGGGCGG855.3217896E-511.17701210
CGCGGGT600.00588327211.08166115
ACAACGC1502.1354936E-910.7658483
TTGACCG2300.010.73670918
Produced by FastQC (version 0.11.5)