##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364319_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2007901
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.866113916971006	31.0	30.0	34.0	26.0	34.0
2	29.8939614054677	31.0	30.0	34.0	26.0	34.0
3	29.929598122616603	31.0	30.0	34.0	26.0	34.0
4	33.300852482268795	35.0	33.0	37.0	27.0	37.0
5	32.920035400151704	35.0	33.0	37.0	26.0	37.0
6	32.8534285305899	35.0	33.0	37.0	26.0	37.0
7	32.59967249381319	35.0	33.0	37.0	25.0	37.0
8	32.491950549354776	35.0	33.0	37.0	25.0	37.0
9	33.248494323176295	37.0	33.0	39.0	17.0	39.0
10	33.38472763348392	37.0	33.0	39.0	19.0	39.0
11	33.33770688893526	37.0	33.0	39.0	19.0	39.0
12	33.30042367626691	37.0	33.0	39.0	18.0	39.0
13	33.339069505916875	37.0	33.0	39.0	19.0	39.0
14	34.285611691014644	38.0	33.0	41.0	18.0	41.0
15	34.31968956636806	38.0	33.0	41.0	19.0	41.0
16	34.26795544202628	38.0	33.0	41.0	18.0	41.0
17	34.23539357767141	38.0	33.0	41.0	18.0	41.0
18	34.192411876880385	38.0	33.0	41.0	18.0	41.0
19	34.10761138123842	39.0	33.0	41.0	18.0	41.0
20	34.03887343051276	39.0	33.0	41.0	18.0	41.0
21	33.98694158725953	39.0	33.0	41.0	18.0	41.0
22	33.93021418884696	39.0	33.0	41.0	17.0	41.0
23	33.684074065404616	39.0	33.0	41.0	10.0	41.0
24	33.606305788980634	39.0	33.0	41.0	2.0	41.0
25	33.43855249835525	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.3097109386753658
1101	2	0.4229372144801111
1101	3	-1.3704527407723646
1101	4	-0.21079619371972314
1101	5	0.5263481590870214
1101	6	0.860925707517147
1101	7	0.9889923913081304
1101	8	1.192275118392942
1101	9	1.80973108788087
1101	10	1.532697642456796
1101	11	1.3922811165198183
1101	12	1.6500702462333265
1101	13	1.313754970319934
1101	14	1.5491456791174727
1101	15	1.4086186183938878
1101	16	1.4767058696880682
1101	17	1.4072711975510046
1101	18	1.3804321471752132
1101	19	1.3352598270617762
1101	20	1.7988677770885815
1101	21	1.6031568274872399
1101	22	1.7985501258679193
1101	23	2.129196838478208
1101	24	1.9620403617311055
1101	25	2.0646535059053335
1102	1	0.3622572376223623
1102	2	0.4489653938162448
1102	3	0.60694170940668
1102	4	0.6217918687505275
1102	5	0.5457343350827273
1102	6	0.7100629545926367
1102	7	0.8552481025067422
1102	8	0.9389563660311708
1102	9	1.6488998089159068
1102	10	1.1711609803650873
1102	11	1.0470813861096602
1102	12	1.2827602228241588
1102	13	1.0504623753704863
1102	14	1.097801901358963
1102	15	1.1613210602431323
1102	16	1.2165520824252098
1102	17	1.1286162349222408
1102	18	1.1941738676753246
1102	19	1.1908317321360542
1102	20	1.3859896274433225
1102	21	1.6386779063700772
1102	22	1.576212530026936
1102	23	1.8825589411480976
1102	24	1.8185549315242895
1102	25	1.6868150831367714
1103	1	-3.0922491275391266
1103	2	-2.818914154489903
1103	3	-2.806681004985773
1103	4	-3.15509618053542
1103	5	-5.463401046192562
1103	6	-9.525927346113662
1103	7	-11.709559532867484
1103	8	-11.855162732272863
1103	9	-12.346165957370587
1103	10	-11.25133983025902
1103	11	-12.249779736358025
1103	12	-12.596428589153337
1103	13	-13.514010437001748
1103	14	-13.850917312547114
1103	15	-13.67651534876255
1103	16	-7.615518322040977
1103	17	-5.382179163552376
1103	18	-4.77606917857959
1103	19	-4.308361168206524
1103	20	-9.07479731234493
1103	21	-5.839121566371809
1103	22	-11.285840393154096
1103	23	-12.571464574000004
1103	24	-13.751574627355218
1103	25	-13.239826959354033
1104	1	0.19293664376786523
1104	2	0.43429398322527746
1104	3	0.4591574715664244
1104	4	0.5267205626201417
1104	5	0.47239927382994296
1104	6	0.5978017952765029
1104	7	0.3361263175659488
1104	8	0.5945216411336176
1104	9	1.0734433338323086
1104	10	0.895714028473904
1104	11	0.8658802661036873
1104	12	0.7872115789463976
1104	13	0.6612018856231359
1104	14	0.7614193792641757
1104	15	0.7811959628022223
1104	16	0.47565468353303686
1104	17	0.37069136800149494
1104	18	0.5151126188215471
1104	19	0.6768861458902933
1104	20	0.986566124204451
1104	21	0.8450338027970119
1104	22	0.7314918148253682
1104	23	1.1518109091108997
1104	24	1.2331311101911595
1104	25	1.3757679835870107
1105	1	0.07362343681871764
1105	2	0.14121242778857734
1105	3	-0.17638659451784733
1105	4	0.03508658151834254
1105	5	0.2198552473645421
1105	6	0.31566346600410355
1105	7	0.40062038464069616
1105	8	0.3262588293826596
1105	9	0.4610307429565381
1105	10	0.13040529845666526
1105	11	0.5007998398046851
1105	12	0.694187966101957
1105	13	0.6551297990955334
1105	14	0.4908299495777584
1105	15	0.3141687644503648
1105	16	0.448725739970115
1105	17	0.46952756379793215
1105	18	0.6037949943687835
1105	19	0.6127382486616355
1105	20	0.8668859475043078
1105	21	0.7730993125927768
1105	22	0.6266969415066939
1105	23	1.0955209188175203
1105	24	1.0232120317259898
1105	25	1.121049871822187
1106	1	-0.20902759746108757
1106	2	0.06355274896505492
1106	3	-0.08204172013962108
1106	4	-1.4622292161298525
1106	5	-0.3893732628719846
1106	6	-0.021688013377740845
1106	7	0.2403702650884938
1106	8	0.28771203235982057
1106	9	0.8250653538294515
1106	10	0.5071923483988314
1106	11	0.567000184768979
1106	12	0.7392061135128856
1106	13	0.31133884991237437
1106	14	-0.037281064347517656
1106	15	0.5299022427449955
1106	16	0.18367299394803638
1106	17	0.1597461501800126
1106	18	0.19960136885123347
1106	19	-0.9652128514229759
1106	20	0.10638200943439102
1106	21	-0.17339576430892123
1106	22	0.3267694902778615
1106	23	0.2205051828868747
1106	24	0.2436690481117978
1106	25	0.6162116862362979
1107	1	0.09493467154900159
1107	2	-0.0699468840736337
1107	3	-0.18982635832746197
1107	4	-0.006840127865316958
1107	5	0.019996732323093624
1107	6	0.0969915743492038
1107	7	-0.05376464349540555
1107	8	-0.09072147735913916
1107	9	0.7407297128414569
1107	10	0.596653443163504
1107	11	0.45079652139413895
1107	12	0.44688000151442964
1107	13	0.24875452909758877
1107	14	0.4995645089129539
1107	15	0.19461749400191763
1107	16	0.15607973996207392
1107	17	-0.10964387936875397
1107	18	0.09864653069230656
1107	19	0.5245419355163037
1107	20	0.39549899267893807
1107	21	0.2744947545045022
1107	22	0.24865295346101846
1107	23	0.5394811099579684
1107	24	0.9223541723220166
1107	25	0.4698108979953943
1108	1	-0.0783235406855205
1108	2	0.03285360418670891
1108	3	0.009328864286459293
1108	4	-0.20685154795442884
1108	5	0.13917669983768377
1108	6	0.06138181940802667
1108	7	-0.25344098497709666
1108	8	0.06222402438834962
1108	9	0.41852406074252
1108	10	0.14092911834276833
1108	11	0.20344326618947406
1108	12	0.35644454259124103
1108	13	-0.02631058241680506
1108	14	-0.033520715769569165
1108	15	-0.4444216271212156
1108	16	-0.8173409302549217
1108	17	-0.984731398169906
1108	18	-0.9485409064675707
1108	19	-0.760565536489274
1108	20	-0.13383801033717901
1108	21	-0.4825048170360162
1108	22	-0.24466315079202872
1108	23	-0.5653491239240154
1108	24	-0.2095227079858617
1108	25	-0.4581955409559342
1109	1	0.15296092756938506
1109	2	0.19515274141506822
1109	3	0.15774432455892295
1109	4	0.16976306269719288
1109	5	0.05382343758221708
1109	6	0.31456353762458633
1109	7	0.4818485967797699
1109	8	0.2636489750373556
1109	9	1.083213307919479
1109	10	0.527764243696673
1109	11	0.42455142752394437
1109	12	0.5662837753144885
1109	13	0.5715377153351824
1109	14	0.2090347089764606
1109	15	-0.02853722357595956
1109	16	0.3989823470585634
1109	17	0.16629455189943343
1109	18	0.17088053534084224
1109	19	0.3991281806419593
1109	20	0.8745021527266701
1109	21	0.7460051059380106
1109	22	0.3016307618065497
1109	23	0.7118297138919303
1109	24	0.4999857268284984
1109	25	0.5165944644757445
1110	1	-0.0521277259536177
1110	2	-0.009806231844862623
1110	3	-0.03502743408290243
1110	4	0.0794359817726118
1110	5	-0.00882851014708308
1110	6	0.011099436534045992
1110	7	0.04798075325334139
1110	8	0.024802501453223158
1110	9	-3.499950644789539
1110	10	-1.3336048249669759
1110	11	-0.4892289432523995
1110	12	0.026276731628620098
1110	13	-0.2975623445907516
1110	14	-0.06810839387636491
1110	15	0.004784461284621955
1110	16	-0.41213561966411305
1110	17	-0.24631733145385226
1110	18	-0.22039757968145324
1110	19	-0.34869284967455627
1110	20	0.10950599535762251
1110	21	-0.13248653514524733
1110	22	0.14942844967571034
1110	23	0.284647911718487
1110	24	0.39231288692629107
1110	25	0.23786584977491998
1111	1	0.3283137268865701
1111	2	0.2694870994285914
1111	3	0.25493359118906866
1111	4	0.18898821240600228
1111	5	0.37624884285678206
1111	6	0.30543470524064276
1111	7	0.3729736642724504
1111	8	0.5573200967926866
1111	9	1.1912120849667716
1111	10	0.6647075086864191
1111	11	0.783674527317082
1111	12	0.9403260161793412
1111	13	0.6607456476729752
1111	14	0.5231272790488148
1111	15	0.5618136991751754
1111	16	0.5809347792939548
1111	17	0.5131151198472921
1111	18	0.5871339491721628
1111	19	0.6098593946479056
1111	20	0.7116807969150472
1111	21	0.7538867813440149
1111	22	0.8551303370068695
1111	23	1.095658810171038
1111	24	0.9863176624549297
1111	25	1.1945360430058685
1112	1	-0.023378330375443568
1112	2	-0.14866778683355264
1112	3	-0.08258296897109574
1112	4	-0.01400083461989965
1112	5	0.125723571408777
1112	6	-0.06744643588063326
1112	7	0.04729170251974324
1112	8	-0.31795560883351115
1112	9	0.43388472154229873
1112	10	0.06264939514857559
1112	11	0.23689398087635283
1112	12	-0.21849804907511583
1112	13	-0.10109796104583779
1112	14	-0.8474499947676222
1112	15	-0.15115104971437887
1112	16	-0.1982264498313384
1112	17	-0.19547279603798273
1112	18	-0.2859174967352729
1112	19	0.0023665781433876987
1112	20	-0.0639481921280165
1112	21	-0.2361418734263978
1112	22	-0.09410517272921481
1112	23	0.12618673520524482
1112	24	0.044772800015650205
1112	25	0.03775365489710225
1113	1	-2.3278215974356264
1113	2	-2.1827134376440256
1113	3	-2.2599853423060594
1113	4	-2.1659898215486706
1113	5	-1.9467082025696705
1113	6	-1.7651847260017028
1113	7	-2.3420149313613052
1113	8	-2.326448624907073
1113	9	-12.911406986729048
1113	10	-5.243990146396108
1113	11	-3.597611076215461
1113	12	-13.937433784272343
1113	13	-5.2036322806649125
1113	14	-4.379188425269117
1113	15	-3.5224538933237852
1113	16	-3.4156270570899103
1113	17	-3.493511771985382
1113	18	-3.8373635283842695
1113	19	-4.537107493364591
1113	20	-17.84627248450876
1113	21	-19.9203708112673
1113	22	-14.922645193605145
1113	23	-21.018848891989613
1113	24	-21.76117955667744
1113	25	-17.634454642492045
1114	1	-0.22709855999040585
1114	2	-0.1896755108558672
1114	3	-0.15790703962098362
1114	4	-0.6203854902035175
1114	5	-0.11471981519771646
1114	6	-0.11505298321316104
1114	7	-0.2371641825256603
1114	8	-0.0709570488780642
1114	9	0.5736403787591584
1114	10	0.01766891840208018
1114	11	-0.2528852594445681
1114	12	-0.32814230593250926
1114	13	-0.2092666067128377
1114	14	-0.24814390790177043
1114	15	-0.23622118671463
1114	16	-0.44525370559464506
1114	17	-0.23001931537794462
1114	18	-0.25106684224856224
1114	19	-0.5192276235250901
1114	20	-0.7597556537655663
1114	21	-1.0004793700768673
1114	22	-0.9548757400453383
1114	23	-0.48322674269029164
1114	24	-0.16258917011726481
1114	25	-0.655643560962865
1115	1	-0.1661929340781505
1115	2	-0.2205584906652973
1115	3	-0.2552638513364833
1115	4	-0.04230553060179432
1115	5	-0.37650030853363603
1115	6	-0.40037478620453015
1115	7	-0.6236989079786817
1115	8	-0.34641073074006457
1115	9	0.11840520139715238
1115	10	-0.30757092689371035
1115	11	-0.07802769376814211
1115	12	-0.12787489333157964
1115	13	-0.20819333262095796
1115	14	-0.27040095129952846
1115	15	-0.4207639357146107
1115	16	-0.32751657027210257
1115	17	-0.30516291863318656
1115	18	-0.46141453707635094
1115	19	-0.30474814489978286
1115	20	-0.8095548702696291
1115	21	-0.21948806926633324
1115	22	-0.6330156658415547
1115	23	-0.3933692120818151
1115	24	-0.0785737651564773
1115	25	-0.06859497314921015
1116	1	-0.5690745310567742
1116	2	-0.5465564309126663
1116	3	-0.4998530015285567
1116	4	-0.3545115454755887
1116	5	-1.3786851774262665
1116	6	-1.2642399150647599
1116	7	-2.258034352101415
1116	8	-1.277772368913741
1116	9	-0.3739642301674877
1116	10	-1.4945466097771174
1116	11	-1.244769582539977
1116	12	-2.2572102574713178
1116	13	-1.8635406911634078
1116	14	-1.2580643356163108
1116	15	-2.6329698744141155
1116	16	-2.767843159896099
1116	17	-3.4361876379499954
1116	18	-2.625177820919255
1116	19	-2.3934424338852125
1116	20	-2.5568577293190238
1116	21	-1.8520720761707707
1116	22	-1.5727121037035268
1116	23	-1.699385114225283
1116	24	-2.300639703139943
1116	25	-1.8328380407180873
1201	1	0.7344518215450471
1201	2	0.7485587926775068
1201	3	0.263159288536567
1201	4	0.5838556071329251
1201	5	0.7911673601041471
1201	6	0.9766366830955349
1201	7	1.0552721423697875
1201	8	1.1695906989489302
1201	9	1.859131605907649
1201	10	1.59747899979984
1201	11	1.4971645272971301
1201	12	1.569646836933245
1201	13	1.5152988843351238
1201	14	1.6706462803837425
1201	15	1.4989579417058394
1201	16	1.3538027287461105
1201	17	1.2964446286360953
1201	18	1.2764522956120459
1201	19	1.3559941769182444
1201	20	1.7096811920071247
1201	21	1.8163115292732783
1201	22	1.7499237018544491
1201	23	1.8981103764716778
1201	24	1.9685385189267564
1201	25	1.9081751004518566
1202	1	0.8782280593780349
1202	2	0.9257136873039293
1202	3	0.8824266621276244
1202	4	1.0463007030704432
1202	5	1.0827709958399154
1202	6	1.054258741737442
1202	7	1.1525891904466832
1202	8	1.2189854731586038
1202	9	1.8046848749158997
1202	10	1.5680013389661482
1202	11	1.5724377813742123
1202	12	1.6825737168243577
1202	13	1.5109861512710054
1202	14	1.6012467722876664
1202	15	1.7222280858495083
1202	16	1.6740043510735632
1202	17	1.608479702214531
1202	18	1.4818539018426975
1202	19	1.5429120184167076
1202	20	1.87882069317682
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2211	5	-0.49676249451620436
2211	6	0.15195519347324904
2211	7	0.3960624610877943
2211	8	0.27513016567976223
2211	9	0.890629201495841
2211	10	0.2955084458569601
2211	11	0.6635139682270648
2211	12	0.681672176310741
2211	13	0.5659248315800838
2211	14	0.49401345428924515
2211	15	0.5443396147046968
2211	16	0.5950679759176722
2211	17	0.37142139606981317
2211	18	0.431098135735958
2211	19	0.12514855322078233
2211	20	0.5874144671666031
2211	21	0.8323413837608982
2211	22	0.7405525539997626
2211	23	1.0580534882077615
2211	24	0.9248352222630558
2211	25	1.0684723180237725
2212	1	-0.09426974445666048
2212	2	-0.05299434316914997
2212	3	0.06407569948740743
2212	4	0.1731911313153347
2212	5	-0.39211633133142243
2212	6	-0.11598061090059986
2212	7	-0.06322380571039332
2212	8	-0.07720203161566985
2212	9	0.47512869495080423
2212	10	0.15234299250968775
2212	11	0.21414397044714661
2212	12	0.04949307715999396
2212	13	-0.2313857550004741
2212	14	-0.16152257012608828
2212	15	-0.03788446578607818
2212	16	0.14280264467515735
2212	17	-0.12278495112922627
2212	18	-0.034496144871724255
2212	19	0.1575831555161855
2212	20	0.4135228593743818
2212	21	0.6627533011287241
2212	22	-0.030427789804505778
2212	23	0.3835717308547757
2212	24	0.3909716149598097
2212	25	0.4168404139764732
2213	1	-0.276595422161126
2213	2	-0.2517589047142508
2213	3	-0.35453212437997905
2213	4	-0.3487088610652549
2213	5	-0.21946184067508057
2213	6	-0.13594929370160713
2213	7	-0.07304923296192811
2213	8	-0.13179644954894343
2213	9	0.4800851055581532
2213	10	-0.18853376559934532
2213	11	0.10787687968018389
2213	12	0.1971195594180557
2213	13	-0.07164890342664165
2213	14	-0.11830144911287022
2213	15	-0.1719745843949454
2213	16	-0.2850894870108789
2213	17	-0.2948845650789593
2213	18	-0.5131081699988584
2213	19	-0.44344470684335136
2213	20	-0.01978381777132654
2213	21	-0.2510875392078873
2213	22	-0.16938877699825383
2213	23	-0.3239576250508307
2213	24	-0.3074871999956841
2213	25	0.06456390265176992
2214	1	-1.0029105623665693
2214	2	-0.9258867409022997
2214	3	-0.9681760967884827
2214	4	-0.8034369018328462
2214	5	-1.0464386377609074
2214	6	-0.9666908285901243
2214	7	-0.8662234318376356
2214	8	-0.8060539751918014
2214	9	-11.057894740967967
2214	10	-4.186190709449068
2214	11	-1.919397124634596
2214	12	-1.2698828083281164
2214	13	-1.097777896925951
2214	14	-1.1917725307710612
2214	15	-1.1491653735378975
2214	16	-1.4962049339786816
2214	17	-1.2753404979392187
2214	18	-1.5055926883758985
2214	19	-1.675183629402973
2214	20	-1.4283113941169674
2214	21	-1.8025109678797548
2214	22	-1.5436278876298601
2214	23	-1.8050927562929928
2214	24	-2.1070406047761736
2214	25	-9.688957143762973
2215	1	-0.4065218371258439
2215	2	-0.05347802250689071
2215	3	-0.05214863141321047
2215	4	-0.18958590079280668
2215	5	-0.13318473295131383
2215	6	-0.15778371970090888
2215	7	-0.24670499095014264
2215	8	-0.18628019774092763
2215	9	0.5985939617276017
2215	10	0.43226764177956767
2215	11	0.576650269258117
2215	12	0.6722359069172015
2215	13	0.495241514882963
2215	14	0.33219094685257744
2215	15	-0.09898322112562141
2215	16	0.29996240871822977
2215	17	0.16667026143542074
2215	18	0.3281304477450675
2215	19	0.3774248531054525
2215	20	0.5800008185442209
2215	21	0.43538298343749915
2215	22	0.37040215077477967
2215	23	0.7761966514936134
2215	24	1.0118389593040575
2215	25	1.1288287270816966
2216	1	-0.764057946324229
2216	2	-0.6469474981144785
2216	3	-0.6734227167778037
2216	4	-0.5581752276820282
2216	5	-1.3825760585940863
2216	6	-1.97270485755255
2216	7	-1.2085413936859517
2216	8	-2.020198410506193
2216	9	-0.7098862067192897
2216	10	-1.1702893603886544
2216	11	-0.7577444639927862
2216	12	-1.8392868718109767
2216	13	-1.6278356223308315
2216	14	-1.7470289574923257
2216	15	-1.4993539277385821
2216	16	-2.5191038370598307
2216	17	-2.777662927094319
2216	18	-2.293217972596331
2216	19	-3.7488702724868688
2216	20	-2.5896670009365685
2216	21	-2.7809991745946263
2216	22	-2.476714605267002
2216	23	-2.1827053855974228
2216	24	-2.2122695984067633
2216	25	-2.3835580318517877
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61582.0
3	6.0
4	875.0
5	8766.0
6	11508.0
7	9742.0
8	7848.0
9	6435.0
10	5571.0
11	5034.0
12	4534.0
13	4259.0
14	4199.0
15	4311.0
16	4479.0
17	4715.0
18	5341.0
19	6753.0
20	8883.0
21	11867.0
22	14717.0
23	17637.0
24	20520.0
25	23343.0
26	26616.0
27	29599.0
28	32943.0
29	38843.0
30	47468.0
31	59057.0
32	73540.0
33	91104.0
34	113889.0
35	143206.0
36	186450.0
37	273210.0
38	639051.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.22645374440561	18.94336265199007	13.300401321531478	24.529782282072844
2	16.130519947188	21.00845818742881	38.784347846008494	24.076674019374693
3	19.115014806529018	25.659321340028228	29.23021986176589	25.99544399167687
4	12.503847452612831	17.328826957768168	36.56902852942143	33.59829706019757
5	13.062745518088315	38.23259359267849	33.97703806480059	14.727622824432615
6	30.89963445205913	37.57946396595857	17.718191875063194	13.802709706919112
7	27.941172807865676	32.34549119878536	21.24276528051957	18.470570712829396
8	26.286452433118022	35.72779327090252	19.386033869664463	18.599720426315
9	26.26105264416514	15.870520901628726	19.522252756559848	38.346173697646286
10	15.278179815328999	30.675584177861303	31.734654884046282	22.311581122763418
11	34.60187493647576	23.28504495024682	22.46206980391688	19.651010309360533
12	23.134577023362148	26.69540551915467	29.85837274341484	20.31164471406834
13	28.731451999695196	21.972666026174515	26.04960278031368	23.246279193816612
14	21.78668248515647	21.566887159281134	28.399120021795248	28.247310333767146
15	23.881091365501852	29.107164202892776	24.26076821930908	22.75097621229629
16	22.61404319665615	28.91219415982484	25.853543372973103	22.620219270545906
17	22.129369152547778	28.05970178989815	27.253560271592736	22.557368785961334
18	21.380841443872775	27.018992584578495	29.616208227546398	21.983957744002335
19	22.92811927784541	26.243855688631108	29.3420132420246	21.486011791498882
20	22.7367323110764	26.09649210194941	29.614999920302214	21.55177566667198
21	23.41082267340216	25.28602751243479	29.445069269214024	21.85808054494903
22	22.92134350162551	25.68194417533879	29.383083650324576	22.013628672711125
23	22.397672586144896	25.46209086716189	29.743709537475805	22.39652700921741
24	22.314701597539568	25.926610302449227	29.716626614037928	22.04206148597328
25	22.3105529737715	25.521172208071018	29.655200952875255	22.513073865282227
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	991.0
1	991.0
2	1513.0
3	2035.0
4	2035.0
5	2035.0
6	5995.5
7	9956.0
8	9956.0
9	9956.0
10	9946.5
11	9937.0
12	9937.0
13	9937.0
14	10852.5
15	11768.0
16	11768.0
17	11768.0
18	19641.0
19	27514.0
20	27514.0
21	27514.0
22	40674.5
23	53835.0
24	53835.0
25	53835.0
26	77203.0
27	100571.0
28	100571.0
29	100571.0
30	117398.0
31	134225.0
32	134225.0
33	134225.0
34	150585.0
35	166945.0
36	166945.0
37	166945.0
38	180227.5
39	193510.0
40	193510.0
41	193510.0
42	209963.5
43	226417.0
44	226417.0
45	226417.0
46	239863.0
47	253309.0
48	253309.0
49	253309.0
50	251269.0
51	249229.0
52	249229.0
53	249229.0
54	229540.0
55	209851.0
56	209851.0
57	209851.0
58	181824.5
59	153798.0
60	153798.0
61	153798.0
62	127247.0
63	100696.0
64	100696.0
65	100696.0
66	78106.0
67	55516.0
68	55516.0
69	55516.0
70	41297.5
71	27079.0
72	27079.0
73	27079.0
74	19224.5
75	11370.0
76	11370.0
77	11370.0
78	8625.0
79	5880.0
80	5880.0
81	5880.0
82	4052.0
83	2224.0
84	2224.0
85	2224.0
86	1516.5
87	809.0
88	809.0
89	809.0
90	605.0
91	401.0
92	401.0
93	401.0
94	215.5
95	30.0
96	30.0
97	30.0
98	17.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02654513345030457
2	0.0011952780540474854
3	1.4940975675593567E-4
4	0.003934456927906306
5	0.033168965999817716
6	0.011454748017955069
7	0.019622481387279553
8	0.027840018008856014
9	0.03431444080161322
10	0.03804968472051162
11	0.039493979035819
12	0.00826733987382844
13	0.003486227657638499
14	0.006175603279245341
15	0.007022258567528977
16	0.00761989759455272
17	0.009412814675623948
18	0.016335466738648968
19	0.017580548044948432
20	0.015787630963877204
21	0.011305338261199133
22	0.01105632199993924
23	0.008914782153104163
24	0.0062752097837492984
25	0.10867069641381721
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2007901.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.261982871175825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3552631870637	40.427394396776215
2	12.616307702118768	13.187065140512297
3	4.138280223544019	6.488241904769495
4	1.7935350177656282	3.7493478550928514
5	0.9956399536537307	2.6017059101854785
6	0.6116164593282775	1.917857335268619
7	0.41473918652244524	1.5172564583428998
8	0.3139233423354311	1.3125005071997253
9	0.23352129452509635	1.0983857305072853
>10	1.3447311540344526	13.091614962676426
>50	0.11839779222712624	4.334601826801379
>100	0.05992307665272865	5.45635284769795
>500	0.002587987817985588	0.9352019474605029
>1k	0.0010543654073274615	1.059134114047748
>5k	1.917028013322657E-4	0.6749644351643266
>10k+	2.8755420199839856E-4	2.148374627496844
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	17476	0.8703616363555773	No Hit
TATCAACGCAGAGTACTTTTTTTTT	13230	0.6588970272936763	No Hit
GGTATCAACGCAGAGTACTTTTTTT	12181	0.6066534156813509	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	8036	0.40021893509689965	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5438	0.2708300857462594	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4857	0.24189439618785988	No Hit
GTGGTATCAACGCAGAGTACATGGG	2820	0.14044517135057955	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2327	0.11589216799035411	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2074	0.10329194517060354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.980325225197856E-5	0.0	0.0	0.0	0.0
9	4.980325225197856E-5	0.0	0.0	0.0	0.0
10	4.980325225197856E-5	0.0	0.0	0.0	0.0
11	4.980325225197856E-5	0.0	0.0	4.980325225197856E-5	0.0
12	4.980325225197856E-5	0.0	0.0	4.980325225197856E-5	0.0
13	4.980325225197856E-5	0.0	0.0	4.980325225197856E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	235	0.0	16.575274	4
ATAACGC	255	0.0	14.900826	3
TATAACG	260	0.0	14.248207	2
GGTATCA	3170	0.0	13.548762	1
GGACCGA	155	0.0	13.48486	6
ACGAAAT	50	0.0015023643	13.297001	12
ATCACGC	165	0.0	13.241418	3
TATCACG	145	0.0	13.101799	2
ACCGAGT	185	0.0	12.83878	8
TACGCAG	75	1.4757048E-5	12.669491	5
GTGGTAT	935	0.0	12.500113	1
TGGTATC	895	0.0	12.311299	2
AGGACCG	235	0.0	12.130363	5
CCGAGTG	190	0.0	12.000583	9
CGAGTGG	185	0.0	11.811383	10
GTATAAC	380	0.0	11.502563	1
CGGGCGG	85	5.3217896E-5	11.177012	10
CGCGGGT	60	0.005883272	11.081661	15
ACAACGC	150	2.1354936E-9	10.765848	3
TTGACCG	230	0.0	10.736709	18
>>END_MODULE