FastQCFastQC Report
Thu 26 May 2016
SRR1364318_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364318_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787000
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT146630.5261212773591676No Hit
TATCAACGCAGAGTACTTTTTTTTT92240.3309651955507714No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA80040.28719052744886975No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN72750.26103336921420883No Hit
GTACTTTTTTTTTTTTTTTTTTTTT65970.23670613562970935No Hit
GGTATCAACGCAGAGTACTTTTTTT61150.21941155364190887No Hit
GAGTACTTTTTTTTTTTTTTTTTTT36470.13085755292429135No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35020.12565482597775385No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA28030.10057409400789379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA2500.014.8256094
TCGCGGT954.84215E-914.01312817
TACCGTA500.001501084513.29887
TATAACG2500.013.29092
AAACGCC2150.013.26752719
CGCATAT1650.013.2407538
CAAAACG2250.012.67854417
AAAACGC2300.012.40247518
AGTGTCG701.0927821E-412.2127468
GGTATCA37050.012.1963391
ATAACGC3050.012.1392313
CGCGCCA1501.4551915E-1112.03181610
CTAGATC2450.012.0249584
CGACCTA802.8419314E-511.88570718
TTATACC4250.011.8515434
TTCGCGG1052.6884663E-711.77335916
TATCACG1552.5465852E-1111.6371952
CGAAATC2650.011.45745513
GAGCGTC752.0766712E-411.3989737
CGTCTCG2850.011.34396117
Produced by FastQC (version 0.11.5)