##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364318_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2787000
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.80716361679225	31.0	30.0	34.0	26.0	34.0
2	29.80751632579835	31.0	30.0	34.0	26.0	34.0
3	29.846101901686403	31.0	30.0	34.0	26.0	34.0
4	33.22018335127377	35.0	33.0	37.0	27.0	37.0
5	32.85440473627556	35.0	33.0	37.0	26.0	37.0
6	32.746202009329025	35.0	33.0	37.0	25.0	37.0
7	32.47791029781126	35.0	33.0	37.0	24.0	37.0
8	32.3561783279512	35.0	33.0	37.0	23.0	37.0
9	33.110804808037315	37.0	32.0	39.0	17.0	39.0
10	33.23697990670972	37.0	32.0	39.0	18.0	39.0
11	33.18567204879799	37.0	33.0	39.0	17.0	39.0
12	33.13327090060997	37.0	32.0	39.0	17.0	39.0
13	33.17754825977754	37.0	33.0	39.0	17.0	39.0
14	34.106921779691426	38.0	33.0	41.0	17.0	41.0
15	34.14362002152853	38.0	33.0	41.0	17.0	41.0
16	34.103323286688195	38.0	33.0	41.0	17.0	41.0
17	34.10119339791891	38.0	33.0	41.0	17.0	41.0
18	34.073042698241835	38.0	33.0	41.0	17.0	41.0
19	33.9960215285253	39.0	33.0	41.0	18.0	41.0
20	33.90387800502332	39.0	33.0	41.0	18.0	41.0
21	33.843068532472195	39.0	33.0	41.0	16.0	41.0
22	33.78930785791173	39.0	33.0	41.0	15.0	41.0
23	33.51978112665949	39.0	33.0	41.0	10.0	41.0
24	33.44176928597058	38.0	33.0	41.0	2.0	41.0
25	33.2859418729817	38.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.17070178363352184
1101	2	0.4134960480000238
1101	3	-1.5601016048400602
1101	4	-0.3723563868536317
1101	5	0.3824098621011913
1101	6	0.7193566599634451
1101	7	0.7885165878701343
1101	8	0.9386610823149724
1101	9	1.6652316187768506
1101	10	1.3095382866574354
1101	11	1.1145355950843978
1101	12	1.2754945523224634
1101	13	1.0255855085895718
1101	14	1.33142834591753
1101	15	1.2553940030636
1101	16	1.2543474088149864
1101	17	1.2206690113915712
1101	18	1.2166460837294153
1101	19	1.1926899867784542
1101	20	1.4722176006042673
1101	21	1.3117119023484989
1101	22	1.3879689188606363
1101	23	1.8423792082735062
1101	24	1.7162503429984213
1101	25	1.8912238738367932
1102	1	0.4832786840775398
1102	2	0.4997449112108505
1102	3	0.5918208107318499
1102	4	0.5386335941170941
1102	5	0.6051326536732873
1102	6	0.7422154841222195
1102	7	0.7828574794185315
1102	8	0.8127021672130681
1102	9	1.4832716031865942
1102	10	0.992151335086568
1102	11	1.0373149395296082
1102	12	1.0568538846928703
1102	13	0.9671121304724792
1102	14	0.9953449380719661
1102	15	1.100722277207467
1102	16	0.9550394172826344
1102	17	0.828124055678586
1102	18	1.0205612238506276
1102	19	1.0835517302882494
1102	20	1.151934989084296
1102	21	1.4100944137546065
1102	22	1.3847354701288737
1102	23	1.6417070697342524
1102	24	1.5914056723424892
1102	25	1.3610105013749845
1103	1	-2.5452302591388936
1103	2	-2.5246204719024803
1103	3	-2.4772567492150372
1103	4	-2.3783819491149636
1103	5	-4.910910461221661
1103	6	-9.955784984517944
1103	7	-10.322047620948567
1103	8	-10.36745482724136
1103	9	-11.765744252472231
1103	10	-9.138646134256557
1103	11	-12.82637325854897
1103	12	-11.441173921290101
1103	13	-12.162349714407629
1103	14	-12.025802449259125
1103	15	-12.572186416067545
1103	16	-6.729928949506402
1103	17	-4.453802055389133
1103	18	-3.796755030152344
1103	19	-3.418639162471262
1103	20	-7.70490325195296
1103	21	-5.034930189853924
1103	22	-11.63851689259861
1103	23	-12.265256142012998
1103	24	-12.868670091150445
1103	25	-13.799252718182927
1104	1	0.1689275846461591
1104	2	0.3872500814420192
1104	3	0.5059930932792369
1104	4	0.5201275089116848
1104	5	0.5764906175004683
1104	6	0.5542923085348974
1104	7	0.5276903817251863
1104	8	0.7462896600583022
1104	9	0.9929285505149963
1104	10	0.8899192575101225
1104	11	0.836410827994662
1104	12	0.7440981410360692
1104	13	0.619502552906475
1104	14	0.767168953847964
1104	15	0.8185914317127754
1104	16	0.29851197432229526
1104	17	0.26913077802359453
1104	18	0.5477092198899172
1104	19	0.6794239012839824
1104	20	0.9440856381310923
1104	21	0.8203289646860839
1104	22	0.4506620800030774
1104	23	1.069242989784719
1104	24	1.1458050690443997
1104	25	1.2377081128326353
1105	1	0.43539872277914426
1105	2	0.46754127746568486
1105	3	0.09663397777431015
1105	4	0.48763185551861454
1105	5	0.5913953891461077
1105	6	0.6789172236765566
1105	7	0.765597349188269
1105	8	0.532226033380276
1105	9	0.8099066238915285
1105	10	0.29361623971854556
1105	11	0.9962993091363117
1105	12	1.111685637852247
1105	13	0.8228253435023021
1105	14	0.5894709285792388
1105	15	0.46510718825431496
1105	16	0.7695019120917976
1105	17	0.6554725177570617
1105	18	0.6990656892979743
1105	19	0.7431539555243702
1105	20	1.0140705020558158
1105	21	0.9422480242936757
1105	22	0.7156653176271277
1105	23	1.254478546523373
1105	24	1.2465521150128112
1105	25	1.3815957941275698
1106	1	-0.13268427001905536
1106	2	0.10932657378790367
1106	3	-0.08170393641040974
1106	4	-1.4076333877489695
1106	5	-0.29374669278670495
1106	6	-0.14698494678328444
1106	7	0.2670950160754231
1106	8	0.3468843444828025
1106	9	0.8468017366476062
1106	10	0.41644961744936637
1106	11	0.5131797429289691
1106	12	0.6374706181897878
1106	13	0.41987411503169625
1106	14	-0.17845959840538228
1106	15	0.29827599077501077
1106	16	0.09225588744840962
1106	17	0.02715451830351867
1106	18	0.056012178426001924
1106	19	-1.2002159996776385
1106	20	-0.03786841028131249
1106	21	-0.3838744054446295
1106	22	0.15846669282323234
1106	23	-0.03783090381293874
1106	24	0.18789259555752125
1106	25	0.45608201971839435
1107	1	-0.07306752240070225
1107	2	-0.22523493212191426
1107	3	-0.3249576730429595
1107	4	-0.2416398708325005
1107	5	-0.16860598277440886
1107	6	-0.16430270125994184
1107	7	-0.3307114711772883
1107	8	-0.5063290552795543
1107	9	0.3572325177445208
1107	10	0.3585396301653816
1107	11	0.37675617452305943
1107	12	0.057721915848794936
1107	13	0.13204396765098636
1107	14	0.12257123272176074
1107	15	0.01533160771023745
1107	16	-0.1559461512833238
1107	17	-0.49018929358283003
1107	18	-0.3487688351030016
1107	19	-0.03231508037222852
1107	20	-0.16494779157417838
1107	21	-0.22239924336754058
1107	22	-0.120423157294546
1107	23	0.044109226682564895
1107	24	0.2134444914946485
1107	25	-0.09868023247563684
1108	1	-0.29705890056761675
1108	2	-0.24533247023238047
1108	3	-0.12530206249666875
1108	4	-0.38095090967460266
1108	5	-0.07751239838020751
1108	6	-0.07560757577276433
1108	7	-0.3489094477387056
1108	8	-0.008232054008651346
1108	9	0.37450240240394805
1108	10	0.1592626956098755
1108	11	0.14148125367545106
1108	12	0.3212866403204444
1108	13	-0.07640021917962514
1108	14	0.006446238097346679
1108	15	-0.35374047571060174
1108	16	-0.9303307188671539
1108	17	-0.8667390288053909
1108	18	-0.816960877866002
1108	19	-0.7745658513796698
1108	20	-0.05145295909461112
1108	21	-0.25652489910289233
1108	22	-0.08087677553405115
1108	23	-0.5925083153095159
1108	24	-0.23518615995541836
1108	25	-0.3954792373240892
1109	1	0.3054727063894198
1109	2	0.2813793904859949
1109	3	0.2710884722248359
1109	4	0.29593515370378043
1109	5	0.4125217230554554
1109	6	0.5643192944359043
1109	7	0.648401069066189
1109	8	0.5282364030821043
1109	9	1.4198256503587459
1109	10	0.7029576515893297
1109	11	0.7389119413317644
1109	12	0.8574836018553924
1109	13	0.8461546776934696
1109	14	0.4577285095109289
1109	15	0.30049310963141096
1109	16	0.7122428661247397
1109	17	0.523138075953419
1109	18	0.4757027880934146
1109	19	0.6594828989493493
1109	20	0.8892459648081257
1109	21	0.8912932148080515
1109	22	0.6605511715895176
1109	23	0.9861700485177991
1109	24	0.6658398207017271
1109	25	0.756129803624674
1110	1	0.15488029505133483
1110	2	0.13809886238201585
1110	3	0.1367530064452609
1110	4	0.2518807664843834
1110	5	0.2827408897057211
1110	6	0.4579334459113511
1110	7	0.39778863933184994
1110	8	0.28145867904454036
1110	9	-3.1966165100933743
1110	10	-1.291482820320006
1110	11	-0.26558712861791633
1110	12	0.11367066790397473
1110	13	-0.20720408914151278
1110	14	0.14481403974895102
1110	15	0.20867874516977025
1110	16	-0.137214321244862
1110	17	-0.07717903770793555
1110	18	0.02780816874808778
1110	19	-0.18149519551800353
1110	20	0.30760132334714285
1110	21	0.0573235469524036
1110	22	0.3161782990411055
1110	23	0.565581754170644
1110	24	0.6359799077290731
1110	25	0.7734657944150172
1111	1	0.1208646950616945
1111	2	0.1283510598125801
1111	3	0.05017571256885489
1111	4	-0.024964032295379468
1111	5	0.12075666730878964
1111	6	0.08559027106748829
1111	7	0.2622779705491425
1111	8	0.30947320439506143
1111	9	1.0015879973536315
1111	10	0.368099823187066
1111	11	0.47165620586380186
1111	12	0.6049790030566129
1111	13	0.33768848297547294
1111	14	0.16442201653923405
1111	15	0.2844827721257204
1111	16	0.051766265335153605
1111	17	0.15846304942898115
1111	18	0.09176253689904001
1111	19	0.2860231651724092
1111	20	0.49751291977049306
1111	21	0.45898968551775
1111	22	0.4732778601295067
1111	23	0.7100518711988073
1111	24	0.6314946818747131
1111	25	0.8679829147327283
1112	1	-0.06980026972500042
1112	2	-0.1900001109124716
1112	3	-0.1271875031810339
1112	4	-0.08826117644305498
1112	5	-0.10721101556936219
1112	6	-0.04339089300693644
1112	7	-0.055681463097961625
1112	8	-0.36096808643400635
1112	9	0.2851054817368279
1112	10	0.08905303820590404
1112	11	0.2662798321007287
1112	12	-0.4388260064494389
1112	13	-0.25705021108192483
1112	14	-1.0082166126342358
1112	15	-0.4312675117105442
1112	16	-0.40426238528591796
1112	17	-0.3670521417095287
1112	18	-0.4676682809975219
1112	19	-0.19645387146717752
1112	20	-0.12416996491012355
1112	21	-0.3892885162626527
1112	22	-0.05871320984789463
1112	23	0.190048104191753
1112	24	0.09455931621733527
1112	25	0.03806728955745342
1113	1	-1.8146965854347314
1113	2	-1.8779319659916425
1113	3	-1.807378471958895
1113	4	-1.6451070485419557
1113	5	-1.523847474358078
1113	6	-1.4663609489323939
1113	7	-1.9304794682637656
1113	8	-1.804455911899474
1113	9	-10.596037986654302
1113	10	-3.693675551499336
1113	11	-2.412154376732879
1113	12	-12.085660242546112
1113	13	-4.236901882080435
1113	14	-3.190760322890977
1113	15	-2.733991111870246
1113	16	-2.6189278867605736
1113	17	-2.6994256242430126
1113	18	-2.9204630270407996
1113	19	-3.5504130715133684
1113	20	-14.23518055192119
1113	21	-17.087938198794134
1113	22	-11.53861878472443
1113	23	-18.024409451315858
1113	24	-18.911245661143763
1113	25	-15.266913472184658
1114	1	-0.19610290577902134
1114	2	-0.15441220456309068
1114	3	-0.1563929950598073
1114	4	-0.5138431179614997
1114	5	-0.26061716188077355
1114	6	-0.13781911181405349
1114	7	-0.09125596080178155
1114	8	-0.06422185558663074
1114	9	0.5732952828286457
1114	10	0.013164993944066339
1114	11	-0.132770886201925
1114	12	-0.21750159218419896
1114	13	-0.11100152973399702
1114	14	-0.15286041070466894
1114	15	-0.05367516306680642
1114	16	-0.22290006992133016
1114	17	-0.1257968343937108
1114	18	-0.20779239767148994
1114	19	-0.48938670368010406
1114	20	-0.789160500473109
1114	21	-0.9169639354983232
1114	22	-0.9141533922417651
1114	23	-0.6049399054042013
1114	24	-0.22321308230757353
1114	25	-0.651436720106453
1115	1	-0.33392693780453087
1115	2	-0.25965989362013175
1115	3	-0.2834826116106548
1115	4	-0.14139184462190713
1115	5	-0.4470880848426333
1115	6	-0.41854129855091315
1115	7	-0.7482663422566986
1115	8	-0.42297823980675986
1115	9	0.16039139904270172
1115	10	-0.21389789402454795
1115	11	-0.13945893702514667
1115	12	-0.2020611649760653
1115	13	-0.27948395227871003
1115	14	-0.4893399108211298
1115	15	-0.3882356458502514
1115	16	-0.31651317790461064
1115	17	-0.34037826828097906
1115	18	-0.4436763470271714
1115	19	-0.3103401986576273
1115	20	-0.6979079009466531
1115	21	-0.1919109672123014
1115	22	-0.8115753183531638
1115	23	-0.47897577417412407
1115	24	-0.07095232441625399
1115	25	3.67638288501837E-4
1116	1	-0.5047451468122048
1116	2	-0.33739774351787943
1116	3	-0.3769075562584554
1116	4	-0.1374225015843038
1116	5	-0.9317337057737198
1116	6	-0.7898577088256751
1116	7	-1.8339084826220713
1116	8	-1.035697411765554
1116	9	-0.004238433180475454
1116	10	-1.107993728370264
1116	11	-0.8377388688921741
1116	12	-1.725514832878698
1116	13	-1.3870768298611011
1116	14	-0.9599967707080808
1116	15	-1.8420016096835141
1116	16	-2.167564505503126
1116	17	-2.778329581365899
1116	18	-2.027047253252963
1116	19	-1.679091730723421
1116	20	-2.085346319320614
1116	21	-1.2954905293332928
1116	22	-1.1791491268308647
1116	23	-1.2195370349543353
1116	24	-1.750376988863497
1116	25	-1.304318320372385
1201	1	0.7276676981087675
1201	2	0.6758080001440661
1201	3	0.3474143866311543
1201	4	0.7715113281428145
1201	5	0.7913473176804402
1201	6	0.9805877071086684
1201	7	1.0183125485429585
1201	8	1.149393136330609
1201	9	1.7836462420888637
1201	10	1.5168536443925689
1201	11	1.4843124548332725
1201	12	1.5544235679585725
1201	13	1.3598035552609744
1201	14	1.362056455370272
1201	15	1.40446264793448
1201	16	1.3418266791300795
1201	17	1.3222888881980168
1201	18	1.3588554239331643
1201	19	1.4043844904505676
1201	20	1.5299460713457975
1201	21	1.6726305235283192
1201	22	1.6820861304218795
1201	23	1.9494036100740288
1201	24	1.9676607019181134
1201	25	2.049354352396378
1202	1	0.6409162567826279
1202	2	0.7188659676143168
1202	3	0.6347555126975557
1202	4	0.6828594921242299
1202	5	0.7637610255739489
1202	6	0.6463933628332441
1202	7	0.7821213519227257
1202	8	0.7858585598410599
1202	9	1.335177326249017
1202	10	0.9446079172450226
1202	11	1.1653592397770183
1202	12	1.2003377619747866
1202	13	0.8404517920116845
1202	14	0.9810507522689704
1202	15	0.9293180679683601
1202	16	1.038355737423899
1202	17	0.9734234159474866
1202	18	1.052257011138721
1202	19	1.0363838479676204
1202	20	1.2182894899856436
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2211	1	-0.04391085307291931
2211	2	0.054028207410475204
2211	3	0.15328905668404147
2211	4	0.062126196067282535
2211	5	-0.6528368306773231
2211	6	-0.0037388986339408348
2211	7	0.12608489792754085
2211	8	0.10052551159529344
2211	9	0.6863849139046891
2211	10	-0.08415191878162886
2211	11	0.4452926334317482
2211	12	0.3912831019395
2211	13	0.29135468739452364
2211	14	0.28737953361970625
2211	15	0.3131507919155041
2211	16	0.31991641829862516
2211	17	0.13827126040091997
2211	18	0.22764721119099818
2211	19	0.023717981170150892
2211	20	0.48090425749882115
2211	21	0.6639868357912277
2211	22	0.5544091877222925
2211	23	0.9629568222038358
2211	24	0.8842148515166954
2211	25	1.0867718253318515
2212	1	-0.13286832554084071
2212	2	-0.09463156418521734
2212	3	-0.09159433760101976
2212	4	0.09503667797116577
2212	5	-0.2932466332640544
2212	6	0.013917547196228952
2212	7	0.03738497446493483
2212	8	-0.16340813126023335
2212	9	0.5379414926798631
2212	10	0.13887194038330364
2212	11	0.29916825802261116
2212	12	0.0851725987320151
2212	13	-0.21084792049284573
2212	14	-0.13306012623884556
2212	15	0.07999934173047762
2212	16	0.09202076306979023
2212	17	-0.19715987364190113
2212	18	-0.14567994587628874
2212	19	-0.026858291058637462
2212	20	0.3065069854652478
2212	21	0.3135062318551576
2212	22	-0.11377604373186756
2212	23	0.26071822650370535
2212	24	0.3784818658967666
2212	25	0.3249468850963382
2213	1	-0.029909995614399776
2213	2	0.028186731487348737
2213	3	-0.1058671508867377
2213	4	-0.07899214762900897
2213	5	-0.08446731634123239
2213	6	0.21806790032069046
2213	7	0.041857069090902144
2213	8	0.1665835115095149
2213	9	0.7111342302303427
2213	10	0.22167499994358053
2213	11	0.4300769968492162
2213	12	0.4789916521949209
2213	13	0.054664013245030674
2213	14	0.19547178454630654
2213	15	0.15338605316858178
2213	16	0.05236005676246691
2213	17	0.04127209715323943
2213	18	-0.21964654396706607
2213	19	0.04167108828332289
2213	20	0.5298055070440242
2213	21	0.2164258874620728
2213	22	0.33457922999252077
2213	23	0.04437715842814782
2213	24	0.3350063367576084
2213	25	0.5465622342201399
2214	1	-0.7567482439109128
2214	2	-0.57634747677427
2214	3	-0.6703078883964615
2214	4	-0.5099172914528367
2214	5	-0.7624135680800919
2214	6	-0.6840851099591774
2214	7	-0.6145940779630656
2214	8	-0.6374546813794595
2214	9	-10.367838829326868
2214	10	-3.751031997321409
2214	11	-1.615270980186125
2214	12	-1.0194827984452104
2214	13	-0.7941629236611121
2214	14	-0.972190179356268
2214	15	-1.030596083794137
2214	16	-1.131331411655239
2214	17	-1.0101747617107861
2214	18	-1.4244709786922627
2214	19	-1.578739369595155
2214	20	-1.1577132814170668
2214	21	-1.7061859149334175
2214	22	-1.5157850451118122
2214	23	-2.037758257010516
2214	24	-2.249077921017129
2214	25	-9.845622944852316
2215	1	-0.37558754045140574
2215	2	-0.026922486261966583
2215	3	0.07482661642454858
2215	4	-0.12410899487612426
2215	5	-0.07227089045546364
2215	6	0.021684117781745726
2215	7	-0.08961860424752643
2215	8	-0.08881882308548228
2215	9	0.8065517141566971
2215	10	0.6841003107633696
2215	11	0.6338241455789912
2215	12	0.7585283442828086
2215	13	0.6626592021181779
2215	14	0.34502345363603126
2215	15	-0.031051768774290167
2215	16	0.284753854779737
2215	17	0.263924773274006
2215	18	0.22550449502327297
2215	19	0.1568706302001317
2215	20	0.33633761829995734
2215	21	0.3818545884734945
2215	22	0.3755983554358764
2215	23	0.7441558324934903
2215	24	0.9169378608398802
2215	25	1.0784792277906803
2216	1	-0.6788773866061995
2216	2	-0.6305690409727696
2216	3	-0.6476747144628732
2216	4	-0.5401374885420225
2216	5	-1.257600324461496
2216	6	-1.7575560357638693
2216	7	-1.1822804405418452
2216	8	-2.096189404366406
2216	9	-0.7344459559261054
2216	10	-1.3115509713824594
2216	11	-0.7420864464412134
2216	12	-1.9240340729928427
2216	13	-1.5350716828432134
2216	14	-1.6383606185798811
2216	15	-1.2510910539590725
2216	16	-2.245416575628873
2216	17	-2.708347445248105
2216	18	-2.056284125485991
2216	19	-3.540705080585937
2216	20	-2.2012850134694197
2216	21	-2.3409876262768137
2216	22	-2.1768837399993153
2216	23	-1.8901454957820825
2216	24	-2.0955162110610743
2216	25	-2.122932434700882
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89728.0
3	7.0
4	1312.0
5	13035.0
6	18249.0
7	14238.0
8	11393.0
9	9251.0
10	7911.0
11	7178.0
12	6358.0
13	6039.0
14	5868.0
15	5925.0
16	6083.0
17	6717.0
18	7608.0
19	9942.0
20	13194.0
21	17512.0
22	21118.0
23	25189.0
24	29068.0
25	33369.0
26	36896.0
27	40714.0
28	45698.0
29	53424.0
30	65410.0
31	81370.0
32	100368.0
33	125263.0
34	156830.0
35	196986.0
36	257179.0
37	381041.0
38	879529.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83946853202069	19.48565183607328	13.178955906605486	23.49592372530054
2	15.62201511468777	21.837537558872434	39.32847211906315	23.211975207376646
3	17.753775032328715	28.461217741252586	29.94905625985829	23.83595096656041
4	11.4660098790842	17.71518010346988	37.38120304367077	33.43760697377514
5	11.851630914258108	40.1367356446596	34.127560114367505	13.884073326714777
6	29.38741793148589	38.798347577202534	18.274580363268903	13.539654128042672
7	27.133282731222042	33.457290561988486	22.31624884036828	17.09317786642119
8	26.697432164804297	35.15363727003938	20.090746016671943	18.05818454848437
9	27.061276103012567	15.28340157263259	20.194764663253682	37.460557661101156
10	15.044688686762816	31.060790971808437	32.126930228217404	21.76759011321134
11	33.31164062225177	24.30932106959304	23.58649792058673	18.79254038756846
12	22.87023949343269	28.104065047652167	30.07467102442014	18.951024434495007
13	28.123492955644096	22.436409904582565	27.506286527884626	21.933810611888713
14	21.88241102996294	21.592114852506935	29.613475483967505	26.911998633562618
15	23.132227834401945	30.023690288058404	25.12526930568294	21.718812571856713
16	21.749496276121423	29.10427757114483	27.026620663175137	22.11960548955861
17	20.803589595083256	29.022316909371266	28.49420519671686	21.679888298828622
18	20.407255846201384	26.745588546135675	31.721377630355498	21.125777977307447
19	21.933397547608042	26.470372911720208	31.448833376876546	20.147396163795207
20	21.73338390941379	26.23958348659872	31.42586866033078	20.601163943656715
21	22.563441175077404	25.320991747874526	31.15862769837613	20.956939378671937
22	22.111769235793084	25.720223087760246	30.9579482012096	21.210059475237074
23	21.505047601720843	25.88312845449336	31.562895955854252	21.048927987931542
24	21.728410743978994	26.06093814253204	31.12576816780093	21.084882945688033
25	21.357764209857837	25.932339517919782	31.68456525783957	21.025331014382807
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2661.0
1	2661.0
2	3662.0
3	4663.0
4	4663.0
5	4663.0
6	14065.0
7	23467.0
8	23467.0
9	23467.0
10	21737.0
11	20007.0
12	20007.0
13	20007.0
14	18740.0
15	17473.0
16	17473.0
17	17473.0
18	32856.5
19	48240.0
20	48240.0
21	48240.0
22	69677.0
23	91114.0
24	91114.0
25	91114.0
26	130253.0
27	169392.0
28	169392.0
29	169392.0
30	195528.5
31	221665.0
32	221665.0
33	221665.0
34	241315.5
35	260966.0
36	260966.0
37	260966.0
38	271426.5
39	281887.0
40	281887.0
41	281887.0
42	296528.0
43	311169.0
44	311169.0
45	311169.0
46	329019.5
47	346870.0
48	346870.0
49	346870.0
50	329960.0
51	313050.0
52	313050.0
53	313050.0
54	281432.0
55	249814.0
56	249814.0
57	249814.0
58	215033.0
59	180252.0
60	180252.0
61	180252.0
62	148229.0
63	116206.0
64	116206.0
65	116206.0
66	90919.5
67	65633.0
68	65633.0
69	65633.0
70	49952.0
71	34271.0
72	34271.0
73	34271.0
74	24417.5
75	14564.0
76	14564.0
77	14564.0
78	11485.5
79	8407.0
80	8407.0
81	8407.0
82	5805.0
83	3203.0
84	3203.0
85	3203.0
86	2271.0
87	1339.0
88	1339.0
89	1339.0
90	989.0
91	639.0
92	639.0
93	639.0
94	342.5
95	46.0
96	46.0
97	46.0
98	24.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02709006099748834
2	0.0015069967707212056
3	1.4352350197344813E-4
4	0.003659849300322928
5	0.032077502691065664
6	0.010907786149982059
7	0.01991388589881593
8	0.027341227125941874
9	0.03484033010405454
10	0.03803372802296376
11	0.04011481880157876
12	0.008719052744886975
13	0.0031575170434158598
14	0.00685324721923215
15	0.007319698600645856
16	0.00914962325080732
17	0.01001076426264801
18	0.016038751345532832
19	0.01779691424470757
20	0.015751704341585935
21	0.011625403659849301
22	0.01054897739504844
23	0.008288482238966631
24	0.006243272335844995
25	0.10699677072120561
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2787000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.474256092074267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.81907506826747	21.975034488104082
2	12.797631914212342	8.055918884800437
3	5.744315928832931	5.4239421185360746
4	3.0026766542755428	3.7802805591344475
5	1.886344076557764	2.9685638271673205
6	1.265956367048685	2.3907020938729366
7	0.8378238303703412	1.8458917257983094
8	0.6694017824883727	1.685513850442403
9	0.4741586757815778	1.3431412430845373
>10	2.9226280425257776	17.35574242537842
>50	0.30558616153725615	6.711875977112217
>100	0.25044706560119157	14.981164356086193
>500	0.017044500048630003	3.617246494363544
>1k	0.006218939206932559	3.274236427764057
>5k	0.0	0.0
>10k+	6.909932452147289E-4	4.590745528355119
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	34411	1.234696806602081	No Hit
TATCAACGCAGAGTACTTTTTTTTT	27036	0.970075349838536	No Hit
GGTATCAACGCAGAGTACTTTTTTT	22546	0.8089702188733404	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	19474	0.6987441693577323	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	11669	0.4186939361320416	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	11514	0.4131324004305705	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	4446	0.1595263724434876	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	4254	0.1526372443487621	No Hit
GTGGTATCAACGCAGAGTACTTTTT	4014	0.14402583423035523	No Hit
GTGGTATCAACGCAGAGTACATGGG	3696	0.1326157158234661	No Hit
GTATAACGCAGAGTACTTTTTTTTT	3220	0.11553641908862576	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.588087549336204E-5	0.0	0.0	0.0	0.0
6	3.588087549336204E-5	0.0	0.0	0.0	0.0
7	3.588087549336204E-5	0.0	0.0	0.0	0.0
8	3.588087549336204E-5	0.0	0.0	0.0	0.0
9	3.588087549336204E-5	0.0	0.0	0.0	0.0
10	3.588087549336204E-5	0.0	0.0	0.0	0.0
11	3.588087549336204E-5	0.0	0.0	3.588087549336204E-5	0.0
12	3.588087549336204E-5	0.0	0.0	3.588087549336204E-5	0.0
13	3.588087549336204E-5	0.0	0.0	3.588087549336204E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	400	0.0	15.438302	2
AACCGTC	95	3.0559022E-10	15.001319	7
TATCACG	200	0.0	14.725765	2
TAACGCA	470	0.0	13.9492855	4
AGTCCGG	70	7.2649345E-6	13.571891	10
ATAACGC	500	0.0	13.301885	3
CGTCCGA	65	5.4611246E-5	13.152406	11
GTCGGTT	305	0.0	13.078187	12
ATCACGC	235	0.0	12.938004	3
GGTATCA	5825	0.0	12.803929	1
CGACCGT	60	4.0685563E-4	12.675971	19
CGGTTAC	60	4.0914232E-4	12.667552	9
TGGTCGG	325	0.0	12.569658	10
CGCGCCA	185	0.0	12.324744	10
CTGGTCG	335	0.0	12.194882	9
TAATCCG	55	0.0030654157	12.093057	4
CGAAAAT	55	0.003075321	12.087849	14
CGGTTTG	340	0.0	11.732323	14
TCACGCA	270	0.0	11.613174	4
AGACGAA	320	0.0	11.578519	10
>>END_MODULE