FastQCFastQC Report
Thu 26 May 2016
SRR1364317_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364317_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553913
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT101590.3977817568570269No Hit
GTACTTTTTTTTTTTTTTTTTTTTT71740.28090228602148937No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN67210.26316479848765406No Hit
TATCAACGCAGAGTACTTTTTTTTT63580.2489513150996138No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA62470.24460504331979988No Hit
GGTATCAACGCAGAGTACTTTTTTT40610.1590108981785989No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA35380.13853251853136736No Hit
GAGTACTTTTTTTTTTTTTTTTTTT32200.12608103721622468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3500.015.1897972
ATAACGC3700.015.1381833
TAACGCA4000.014.7342254
GGTATCA26300.012.8145871
ATCACGC1302.0008883E-1012.4144953
GTATAAC5450.012.0194491
ACGGCCC1701.8189894E-1211.74380115
AACGCCG658.0227386E-411.6929626
TAGACAG3800.011.5072055
TTGACCG2950.011.27935818
CGCGGTT1354.6802597E-911.26742318
CGCATAT1104.9687515E-711.22790058
GAACAAA11150.011.0687991
CGCGCCA951.3604802E-511.00039910
GCTAGAT951.3714916E-510.9924013
ACCAGTT3900.010.94729712
GTTCGCG700.001482487710.86544216
TCACGCA1752.0008883E-1110.8639464
CATGCGA700.001491272110.857756
GTATCAA62200.010.8215091
Produced by FastQC (version 0.11.5)