##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364317_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2553913
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.1133351057769	30.0	26.0	31.0	7.0	34.0
2	26.023481222735466	30.0	26.0	31.0	7.0	34.0
3	25.471069687965095	30.0	24.0	31.0	7.0	34.0
4	29.164663400828456	35.0	28.0	37.0	7.0	37.0
5	29.781365692566663	35.0	30.0	37.0	2.0	37.0
6	29.87631371937885	35.0	30.0	37.0	2.0	37.0
7	29.513026872880946	35.0	30.0	37.0	2.0	37.0
8	29.0200715529464	35.0	28.0	37.0	2.0	37.0
9	30.063051873732583	35.0	28.0	39.0	2.0	39.0
10	29.351028793854763	35.0	27.0	39.0	2.0	39.0
11	30.057568523281724	35.0	28.0	39.0	2.0	39.0
12	29.55047568182628	35.0	27.0	39.0	2.0	39.0
13	29.157800598532525	35.0	27.0	39.0	2.0	39.0
14	29.449234175165717	36.0	25.0	40.0	2.0	41.0
15	30.181335072886196	36.0	27.0	40.0	2.0	41.0
16	30.53160307340148	36.0	27.0	40.0	2.0	41.0
17	30.68974471722412	37.0	29.0	40.0	2.0	41.0
18	30.512230447944	37.0	28.0	40.0	2.0	41.0
19	30.499690083413178	37.0	27.0	40.0	2.0	41.0
20	30.378638583225037	37.0	27.0	40.0	2.0	41.0
21	30.283631823010417	37.0	27.0	40.0	2.0	41.0
22	30.350062825162798	37.0	28.0	40.0	2.0	41.0
23	30.342471728676742	37.0	28.0	40.0	2.0	41.0
24	30.219281549528116	37.0	28.0	40.0	2.0	41.0
25	30.02161976543445	37.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.4455730292142768
1101	2	0.730179643518234
1101	3	1.337733484452876
1101	4	0.75231201568252
1101	5	0.16123739398260284
1101	6	-0.021614163041924428
1101	7	0.3871774368434906
1101	8	0.632890863617174
1101	9	0.597390067547316
1101	10	0.8424211728884075
1101	11	0.3777667924775798
1101	12	0.5419199316073957
1101	13	0.46367282832452617
1101	14	1.1613652098492224
1101	15	0.4298714288170977
1101	16	-0.11185103512053729
1101	17	-0.7054584882698656
1101	18	-6.112434369428115
1101	19	-7.6620614692250655
1101	20	-8.610622401073822
1101	21	-10.405109766281477
1101	22	-10.727531952178289
1101	23	-12.144030406719455
1101	24	-12.040721889549925
1101	25	-12.340282472275828
1102	1	1.8278631969318795
1102	2	1.990328364998316
1102	3	2.6847246240555975
1102	4	2.1976297105381803
1102	5	1.5221192237863939
1102	6	1.446239619816584
1102	7	1.8669710888135107
1102	8	2.0065425814513986
1102	9	2.16768177204154
1102	10	-1.309515006106441
1102	11	0.9110752419698152
1102	12	1.8592856617975961
1102	13	2.372725219746574
1102	14	2.841543647833646
1102	15	2.5040385277986843
1102	16	2.058258582445447
1102	17	1.7683317762039366
1102	18	2.015073700450447
1102	19	1.664769127143078
1102	20	2.1674520785528237
1102	21	2.240212766384033
1102	22	2.1720952019337467
1102	23	2.1396410071767917
1102	24	2.341164398244178
1102	25	2.338536232343973
1103	1	-1.5769455507255294
1103	2	-0.9479075440009588
1103	3	-3.571339975408314
1103	4	-1.6274750059991945
1103	5	-4.353895276000813
1103	6	-8.636794434524017
1103	7	-9.068330517213383
1103	8	-3.313568966860071
1103	9	-8.556781205715996
1103	10	-6.008804949585645
1103	11	-9.712979322625912
1103	12	-4.408827872581536
1103	13	-1.2495934022509942
1103	14	-1.0730069110278713
1103	15	-1.7435554184281585
1103	16	-1.8946077761069624
1103	17	-2.0902206343296896
1103	18	-1.4748391664307547
1103	19	-7.103404138594268
1103	20	-3.1630377932487512
1103	21	-7.96610494471032
1103	22	-4.439807764474832
1103	23	-3.790512502413822
1103	24	-9.687553318823309
1103	25	-6.108267393349472
1104	1	1.4067338621580845
1104	2	1.3549831620825792
1104	3	2.2470255097124827
1104	4	1.573879948995689
1104	5	1.1363363793822572
1104	6	1.056875301428736
1104	7	1.1989069775695675
1104	8	1.6519782044843936
1104	9	1.720102748676851
1104	10	2.0019486321714943
1104	11	1.5899455481944464
1104	12	1.8730524437285254
1104	13	2.1574545059437433
1104	14	2.569905106464475
1104	15	1.6208290438188833
1104	16	0.8557043233807491
1104	17	0.6214917761494689
1104	18	0.605911497473187
1104	19	1.0448569686386833
1104	20	-9.545664378215527
1104	21	-3.6787213356583273
1104	22	-1.2491500655898946
1104	23	-0.13875920505503458
1104	24	0.4058632287284638
1104	25	0.9383711658958767
1105	1	1.2326344120559831
1105	2	1.247859802472778
1105	3	1.9247179031378856
1105	4	1.2776045580128077
1105	5	1.0860253498774313
1105	6	1.0907016727259915
1105	7	1.295747945435206
1105	8	1.4280056030385282
1105	9	1.4066269443732509
1105	10	2.1768127633762653
1105	11	1.6211325607055187
1105	12	2.001010340931053
1105	13	1.7529359357916192
1105	14	2.523495310687977
1105	15	1.7692279927601753
1105	16	1.1291531900545948
1105	17	0.7085108988441355
1105	18	1.2225289547951768
1105	19	1.225650967554376
1105	20	1.1256143658033189
1105	21	1.3279875698162833
1105	22	1.0803509837686818
1105	23	1.1725899243834874
1105	24	1.1745009138302152
1105	25	1.1268110313210684
1106	1	1.2060616073615869
1106	2	1.4250135647410431
1106	3	1.9669557906593091
1106	4	1.630280857540253
1106	5	0.8206478248216058
1106	6	0.7004390409557253
1106	7	1.1853056387415357
1106	8	1.1821476033501028
1106	9	1.4764087120421507
1106	10	-2.1170595947272837
1106	11	-0.029827479799230616
1106	12	0.8313830625387801
1106	13	1.7425098569631423
1106	14	0.8127226046589975
1106	15	1.4456846059397996
1106	16	1.0168946606886493
1106	17	-5.620006653214855
1106	18	-1.4889954259494864
1106	19	0.03995940085135885
1106	20	0.6837601050150326
1106	21	1.0105627372878523
1106	22	1.0736060338803313
1106	23	0.5150692825389633
1106	24	0.9302962090475404
1106	25	0.9273783574881769
1107	1	1.0203349009486828
1107	2	1.3097023466208384
1107	3	1.790562124578642
1107	4	1.346972047124698
1107	5	0.7055257834957125
1107	6	0.9225921531369288
1107	7	0.9650372879555285
1107	8	1.198723503373035
1107	9	1.3439343177900689
1107	10	-1.8317147451769742
1107	11	0.3168192500537579
1107	12	0.6817948564698355
1107	13	1.5663009089644646
1107	14	2.0375321539468807
1107	15	1.7341906825277356
1107	16	-5.15738844206556
1107	17	-1.5364538875392952
1107	18	0.21174663117298564
1107	19	1.0726752025278365
1107	20	1.503520799555023
1107	21	1.6238153042322345
1107	22	1.1376047981800568
1107	23	0.881480528685362
1107	24	1.122595375905295
1107	25	1.347864866698604
1108	1	-0.3010776567947815
1108	2	-2.951897904071835
1108	3	0.02083621606890773
1108	4	-0.5347845348138733
1108	5	-0.9118399590243271
1108	6	-0.4081877196076533
1108	7	-0.04552577833233684
1108	8	0.19362719518683846
1108	9	-0.38077366795209855
1108	10	-3.246349620596785
1108	11	-1.434573814953012
1108	12	-5.754945623865357
1108	13	-1.6799103787367713
1108	14	-5.975233704625278
1108	15	-2.667408236831111
1108	16	-7.472797061070477
1108	17	-3.2982223761237357
1108	18	-1.828626066938277
1108	19	-0.5799349653458208
1108	20	-0.5265697311447823
1108	21	-0.031820131352560566
1108	22	-0.18810350909178908
1108	23	-0.14551565566166857
1108	24	0.06687999749249585
1108	25	-0.18406171149716144
1109	1	-9.316670783991995
1109	2	-6.560410074505036
1109	3	-5.573471119932304
1109	4	-5.344501157608594
1109	5	-2.7464040637513314
1109	6	-1.6989730605063151
1109	7	-1.2356520476622492
1109	8	-0.9476930443763116
1109	9	-5.882104650146125
1109	10	-1.9172080634859334
1109	11	-1.5334810065613524
1109	12	-6.461570018817067
1109	13	-8.0530128033957
1109	14	-8.229189622624023
1109	15	-4.181912339126761
1109	16	-2.6354169853014326
1109	17	-2.1872079542038065
1109	18	-1.59554881179211
1109	19	-1.3908997677235924
1109	20	-1.10031441875255
1109	21	-0.9153375118376914
1109	22	-1.113113246926936
1109	23	-1.4233382012930385
1109	24	-1.127217819991941
1109	25	-1.0467522195268444
1110	1	-2.4660068706320217
1110	2	-2.422198139217649
1110	3	-1.678076173933757
1110	4	-2.4247578687936553
1110	5	-5.201406514986605
1110	6	-3.950202095016735
1110	7	-8.851590722212485
1110	8	-9.84770671186996
1110	9	-11.414572969787205
1110	10	-9.148114426298466
1110	11	-11.85457975420297
1110	12	-6.65618086664735
1110	13	-4.915703814047433
1110	14	-4.382519588603849
1110	15	-9.777186060399025
1110	16	-11.384780406928758
1110	17	-12.282729175958892
1110	18	-8.99919090173016
1110	19	-12.25530307763401
1110	20	-13.209925043743265
1110	21	-14.152080488677232
1110	22	-14.707435888912883
1110	23	-15.388716062924523
1110	24	-15.610266859553395
1110	25	-16.309556729799752
1111	1	-0.5731300693090837
1111	2	0.09353597370462907
1111	3	-2.5766516523178815
1111	4	-3.103612699882717
1111	5	-1.0180926713768734
1111	6	-0.5810035360750874
1111	7	-0.10719136005798546
1111	8	0.1920865789403372
1111	9	-4.162041668109907
1111	10	-4.29777187964125
1111	11	-1.359884502608356
1111	12	-6.279361235180307
1111	13	-1.347786261484238
1111	14	0.08316077992910564
1111	15	0.16343170477933455
1111	16	0.04356532895595677
1111	17	0.1677834628315651
1111	18	0.48224306944867124
1111	19	0.556696095529233
1111	20	0.6518473399089899
1111	21	0.7636689797848533
1111	22	0.30108702627729045
1111	23	0.16921093920162988
1111	24	0.45985303177663184
1111	25	0.5982917228343148
1112	1	-0.046933203701524207
1112	2	0.4125874264024958
1112	3	-2.3658673385923947
1112	4	-0.23427857937319985
1112	5	-0.3236977236167462
1112	6	0.055648460489962304
1112	7	0.44412000390365947
1112	8	-3.148699881629291
1112	9	-0.5638843759959826
1112	10	-3.1926194537073727
1112	11	-0.6477870358844378
1112	12	0.5715458064684711
1112	13	0.8922702720780933
1112	14	1.5914082394968752
1112	15	0.972931490094922
1112	16	0.6432558255925684
1112	17	0.39128425000775025
1112	18	0.3829085911317982
1112	19	0.6048229740178535
1112	20	0.7453799719399044
1112	21	0.6497627968737305
1112	22	0.5404297341633644
1112	23	0.35693456879945273
1112	24	0.5582196411941212
1112	25	0.631669782087215
1113	1	-0.9992719400073256
1113	2	-0.563511291751972
1113	3	-2.982268652719256
1113	4	-1.5643024306650979
1113	5	-1.2470159123942821
1113	6	-1.1691063397439372
1113	7	-0.9007086191223372
1113	8	-8.358130088469611
1113	9	-8.99869785446618
1113	10	-3.141826347523928
1113	11	-9.207383722159708
1113	12	-6.942624959927219
1113	13	-2.641466485658821
1113	14	-0.8486001352026769
1113	15	-6.850816686009885
1113	16	-2.648975626015872
1113	17	-1.7672713172305734
1113	18	-1.1149554915526814
1113	19	-0.7972048469554203
1113	20	-6.000646565158721
1113	21	-2.2552005960970547
1113	22	-1.4992829435453636
1113	23	-1.529508010091508
1113	24	-7.524927290511496
1113	25	-4.602934882288398
1114	1	-4.131759613480277
1114	2	-3.802273993777895
1114	3	-0.6383929923494271
1114	4	-0.4431533175200393
1114	5	-0.4441774796795386
1114	6	-0.5602592850479624
1114	7	-0.3908082477266568
1114	8	0.08199788606497549
1114	9	-0.2574585538314267
1114	10	-3.084706474730524
1114	11	-1.1766626438549217
1114	12	-4.430419270112253
1114	13	-1.12230531438842
1114	14	-5.576245435387207
1114	15	-1.8572261412786517
1114	16	-1.0062182935297805
1114	17	-0.7804339174671284
1114	18	-0.25857317760625875
1114	19	-0.14815061475368907
1114	20	0.10587089073945322
1114	21	0.23364605204374556
1114	22	0.07466925460059315
1114	23	-0.2830424050357685
1114	24	-0.191644086435101
1114	25	0.014548270140426922
1115	1	0.4815596863695184
1115	2	0.6242746484848851
1115	3	1.3005042053653568
1115	4	0.2856712849134375
1115	5	-0.10536973038314201
1115	6	-0.4744827404315721
1115	7	-2.4953971875893686
1115	8	-0.2735709142526517
1115	9	-0.11017453728740279
1115	10	0.6468647448128202
1115	11	-0.3386452613386872
1115	12	0.3511942442342004
1115	13	0.6645161524916041
1115	14	1.2763587953887843
1115	15	0.3800622805884082
1115	16	0.17522363533536023
1115	17	-0.26615016653817136
1115	18	-0.07734156589661367
1115	19	0.24605545996466205
1115	20	0.19800183619026157
1115	21	0.3547940551644899
1115	22	-0.04962679606432374
1115	23	-0.08616842596475394
1115	24	-0.13029302198503956
1115	25	0.10605011510082107
1116	1	0.13590877324774908
1116	2	0.25931613063719894
1116	3	1.1677998474006337
1116	4	0.41814571080405827
1116	5	-0.3674893908926329
1116	6	-1.3355164930495533
1116	7	-4.938733399308585
1116	8	-1.2404170323416999
1116	9	-1.3862190377000765
1116	10	-0.5764358580302904
1116	11	-2.3008622981334206
1116	12	-0.6036275775953044
1116	13	-6.715349420734139
1116	14	-1.8188387356572306
1116	15	-1.5578071067924775
1116	16	-0.9273328110701407
1116	17	-0.6293013270158241
1116	18	-0.5680820550369106
1116	19	-0.2630679273734984
1116	20	-0.17559463955361565
1116	21	-0.16195437549983893
1116	22	-0.31324272632261696
1116	23	-0.2425946799968628
1116	24	-0.21047537668446026
1116	25	-0.2679378259627221
1201	1	2.3401764229536326
1201	2	2.5115541449834247
1201	3	3.148699927902893
1201	4	2.736207225516747
1201	5	2.2572028324287636
1201	6	1.9417767199862084
1201	7	2.2613686732098195
1201	8	2.2621820978677043
1201	9	2.788420804796992
1201	10	2.852926240223667
1201	11	2.519062268902303
1201	12	2.76302686401824
1201	13	2.8157943121333844
1201	14	3.4903549429413587
1201	15	3.0529914104096605
1201	16	2.543494130810892
1201	17	2.37967922370661
1201	18	2.4143792658295347
1201	19	2.3357682513838647
1201	20	2.4713020755307475
1201	21	2.3173908155628666
1201	22	1.4788700546557365
1201	23	0.9872625043006771
1201	24	1.5307657541920747
1201	25	1.57691274707949
1202	1	2.246860994761409
1202	2	2.433080143772557
1202	3	3.1598808169244776
1202	4	2.685769453127701
1202	5	1.991707288149339
1202	6	1.938413336617785
1202	7	2.1467297818879736
1202	8	2.5047393918685437
1202	9	2.6997989870778056
1202	10	-0.8402439990535484
1202	11	1.3417111642582427
1202	12	2.4586189643603404
1202	13	2.8924333453836297
1202	14	3.382257799985574
1202	15	3.0076611423887485
1202	16	2.67078120784668
1202	17	2.1792341311366883
1202	18	2.3697305737764403
1202	19	2.5505924168033296
1202	20	2.5999305037888156
1202	21	2.6021180413293905
1202	22	2.3841574628577895
1202	23	2.0551754344252373
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2211	18	0.4629127989862347
2211	19	0.7418032276428868
2211	20	0.8984539114078842
2211	21	0.9305814281672724
2211	22	0.7473838923040361
2211	23	0.6542008207888514
2211	24	0.8458414229490003
2211	25	0.8212950455022963
2212	1	0.9568715429523387
2212	2	0.8762232018499105
2212	3	1.6362087052611187
2212	4	1.1351664751882886
2212	5	0.7243254120216349
2212	6	0.6226153047432561
2212	7	0.7740400303288197
2212	8	1.0957107367910233
2212	9	0.9751271322303801
2212	10	1.7852739909113104
2212	11	0.9949958470122482
2212	12	1.31125523184037
2212	13	1.2833924731306716
2212	14	-4.368229538269549
2212	15	-0.9930636505356389
2212	16	-0.48859637057340066
2212	17	0.06688745712347455
2212	18	0.31475380642365636
2212	19	0.7709321923472459
2212	20	1.14092872823759
2212	21	1.1120791871148086
2212	22	0.8344716432451413
2212	23	0.7801636827903167
2212	24	0.8093698679022658
2212	25	0.841531373488138
2213	1	0.8003530975425619
2213	2	1.0408491442558692
2213	3	1.5943527871937704
2213	4	1.0980706149526505
2213	5	0.551043473647173
2213	6	0.38190804365114417
2213	7	0.5054057542594386
2213	8	-4.920422670042274
2213	9	-1.5966467365523798
2213	10	0.2938809135515008
2213	11	0.36869485366245414
2213	12	0.8503279990039339
2213	13	-8.010748710614834
2213	14	-2.0710567561419246
2213	15	-0.48478324157656516
2213	16	0.05077028641063919
2213	17	0.15413658898768645
2213	18	0.37522771613753747
2213	19	0.630660209476698
2213	20	0.9622015759430269
2213	21	0.9979087134141515
2213	22	0.5875788871725902
2213	23	0.6305871878142817
2213	24	0.8223206603374891
2213	25	0.9928625700411224
2214	1	-1.1141202110345176
2214	2	-0.8603558706283927
2214	3	-7.155125827850881
2214	4	-2.974715759956368
2214	5	-2.011695153949944
2214	6	-1.29945824644156
2214	7	-1.0341295539079418
2214	8	-6.4768551002954595
2214	9	-2.365266499275915
2214	10	-0.5500091848197286
2214	11	-0.8353335942339157
2214	12	-0.47647560067702344
2214	13	-6.448098451502311
2214	14	-1.9354704078197287
2214	15	-1.209179481067146
2214	16	-1.045544878536603
2214	17	-1.0369561183786047
2214	18	-0.8394471728298072
2214	19	-0.5193140209220601
2214	20	-0.33011646779010917
2214	21	-0.2251001626607625
2214	22	-0.3562477406163005
2214	23	-0.45025864350324696
2214	24	-0.3215791356849458
2214	25	-0.3072232378277029
2215	1	0.3046464890754663
2215	2	0.6035849933124737
2215	3	-2.556912114711796
2215	4	-0.45807202092456833
2215	5	-0.030014678985885723
2215	6	-0.38704319074292215
2215	7	0.16550032855775854
2215	8	0.623993222194656
2215	9	0.8685919286123607
2215	10	1.5347273887228141
2215	11	0.7681152047372706
2215	12	0.4762467542841371
2215	13	1.1829936624256234
2215	14	1.3369433377283464
2215	15	0.8462455765967185
2215	16	0.6305166020024906
2215	17	0.42918235074493793
2215	18	0.5132205350617838
2215	19	0.4928645181221505
2215	20	0.8118378286417993
2215	21	1.059268189618038
2215	22	0.8111872604007893
2215	23	0.6042636169891615
2215	24	0.4171730493358936
2215	25	0.43725499471023
2216	1	-0.17585430723583784
2216	2	-0.3699225534322892
2216	3	0.8177250620984537
2216	4	-0.7135413514514788
2216	5	-0.906529996070212
2216	6	-1.1609040060625873
2216	7	-1.7711066231670571
2216	8	-6.824246038810525
2216	9	-2.6831492528423837
2216	10	-1.3245695597405458
2216	11	-1.8451087184492287
2216	12	-0.6378065991335014
2216	13	-0.05348563292012898
2216	14	0.6268195928572986
2216	15	0.1480666693756696
2216	16	-0.4402723906328916
2216	17	-0.5571908071197136
2216	18	-0.40945197757216434
2216	19	-0.1588800256604017
2216	20	-0.22471297220864628
2216	21	-0.3514352766056952
2216	22	-0.4673385045568068
2216	23	-0.7681673123918671
2216	24	-0.6679013533745888
2216	25	-0.6989149104385035
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	248536.0
3	6.0
4	1513.0
5	10837.0
6	16679.0
7	14659.0
8	12307.0
9	10439.0
10	9550.0
11	8825.0
12	8264.0
13	8059.0
14	8239.0
15	8273.0
16	8417.0
17	9122.0
18	10533.0
19	13318.0
20	18680.0
21	23593.0
22	29433.0
23	34070.0
24	38630.0
25	43577.0
26	48405.0
27	52828.0
28	57526.0
29	66853.0
30	75902.0
31	85482.0
32	105626.0
33	133247.0
34	166405.0
35	206065.0
36	257819.0
37	303339.0
38	398857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.86449110068942	17.450512794023314	12.097178091091891	23.587818014195374
2	14.314625340618187	21.261095501149175	41.13925268851206	23.28502646972058
3	15.76445694952649	28.92128476884674	30.14318860211184	25.17106967951493
4	10.556291526020352	16.94634853884981	37.389290136843364	35.10806979828647
5	12.203912965243143	39.92021316302083	33.64574141490019	14.230132456835824
6	30.332552303292537	37.55764955900665	18.59216684592268	13.517631291778137
7	28.122349248836027	33.347549213649756	21.588227994445596	16.94187354306862
8	26.596813324128465	34.982473832485496	20.7411962718704	17.679516571515638
9	27.662780391622483	16.682184801547862	19.4639037448905	36.19113106193915
10	13.067108038457532	31.67914577260048	33.253183990312884	22.0005621986291
11	33.941892908123094	24.789112921435457	22.921101666794293	18.347892503647156
12	22.488600075083212	27.783144604297338	30.086887612369566	19.641367708249884
13	27.168348214250653	23.42097223738368	26.89607022840094	22.514609319964723
14	20.702395916657142	22.1499673478652	29.30810852839204	27.83952820708561
15	22.96077656056798	30.33446590257182	24.14516414662485	22.55959339023535
16	22.40423468245772	30.01339045547183	25.27540750572334	22.306967356347116
17	22.03330333457412	29.65004327046159	26.241414622117727	22.075238772846568
18	21.27480368697034	28.47652136217172	28.710785495571127	21.53788945528681
19	22.76601860316084	27.716612792264705	28.268354207700742	21.249014396873715
20	22.89252663886617	27.560539658596024	28.16411658268681	21.382817119850998
21	23.48421961155121	26.846294060963416	28.01826876333619	21.65121756414918
22	23.07298419936182	27.214221595331196	27.61220228021139	22.100591925095596
23	22.436669408718153	27.521532302871694	27.890236791524902	22.151561496885254
24	22.419077940885433	27.376771348062235	28.007875450395968	22.19627526065636
25	22.30022384839405	27.409040239703504	28.318215746503572	21.972520165398873
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14106.0
1	14106.0
2	12664.0
3	11222.0
4	11222.0
5	11222.0
6	16429.0
7	21636.0
8	21636.0
9	21636.0
10	20752.0
11	19868.0
12	19868.0
13	19868.0
14	21488.5
15	23109.0
16	23109.0
17	23109.0
18	32709.0
19	42309.0
20	42309.0
21	42309.0
22	57033.5
23	71758.0
24	71758.0
25	71758.0
26	96427.5
27	121097.0
28	121097.0
29	121097.0
30	145290.5
31	169484.0
32	169484.0
33	169484.0
34	190600.5
35	211717.0
36	211717.0
37	211717.0
38	232042.5
39	252368.0
40	252368.0
41	252368.0
42	273295.5
43	294223.0
44	294223.0
45	294223.0
46	306760.0
47	319297.0
48	319297.0
49	319297.0
50	309773.5
51	300250.0
52	300250.0
53	300250.0
54	275816.5
55	251383.0
56	251383.0
57	251383.0
58	218770.0
59	186157.0
60	186157.0
61	186157.0
62	152156.5
63	118156.0
64	118156.0
65	118156.0
66	91989.0
67	65822.0
68	65822.0
69	65822.0
70	49313.5
71	32805.0
72	32805.0
73	32805.0
74	23662.5
75	14520.0
76	14520.0
77	14520.0
78	11117.0
79	7714.0
80	7714.0
81	7714.0
82	5334.5
83	2955.0
84	2955.0
85	2955.0
86	2126.5
87	1298.0
88	1298.0
89	1298.0
90	916.0
91	534.0
92	534.0
93	534.0
94	313.0
95	92.0
96	92.0
97	92.0
98	62.5
99	33.0
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.319274775609036
2	0.32005788764143495
3	0.33121723410311943
4	0.333644881403556
5	0.33795199758175004
6	0.2848961573867238
7	0.2922965660928935
8	0.29112189804429517
9	0.29092612003619545
10	0.4043990535307977
11	0.29006469680055663
12	0.2899472299956968
13	0.2839172673462252
14	0.2886550951422386
15	0.2817637092571282
16	0.2867364706628613
17	0.2796101511680312
18	0.28250766568790714
19	0.28383895614298527
20	0.47895131901517396
21	0.2847003793786241
22	0.28610998103694213
23	0.28027579639557026
24	0.2844654457689044
25	0.2780047715016134
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2553913.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.16789590790464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.16069796219399	54.4259000022828
2	7.627938800419541	9.636816882735369
3	2.5165607528705567	4.768975430497364
4	1.146302540994414	2.896380783540069
5	0.6343268444891281	2.0034546042139425
6	0.38504673503981174	1.459355524720402
7	0.2637227225889659	1.1661166642334306
8	0.19872989286628678	1.0042679349093313
9	0.14674056205369398	0.8342363294347647
>10	0.7945255334924234	9.364098938405764
>50	0.07413895381054825	3.2749563147083793
>100	0.04737512398266424	5.5090127147768255
>500	0.002637583198695802	1.0880861011190497
>1k	9.419939995306422E-4	1.1141090870916168
>5k	2.511983998748379E-4	1.0512334273785953
>10k+	6.279959996870947E-5	0.4029992599524207
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10159	0.3977817568570269	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7174	0.28090228602148937	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6721	0.26316479848765406	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6358	0.2489513150996138	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6247	0.24460504331979988	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4061	0.1590108981785989	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3538	0.13853251853136736	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3220	0.12608103721622468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	3.915560161994555E-5	0.0
13	0.0	0.0	0.0	3.915560161994555E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	350	0.0	15.189797	2
ATAACGC	370	0.0	15.138183	3
TAACGCA	400	0.0	14.734225	4
GGTATCA	2630	0.0	12.814587	1
ATCACGC	130	2.0008883E-10	12.414495	3
GTATAAC	545	0.0	12.019449	1
ACGGCCC	170	1.8189894E-12	11.743801	15
AACGCCG	65	8.0227386E-4	11.692962	6
TAGACAG	380	0.0	11.507205	5
TTGACCG	295	0.0	11.279358	18
CGCGGTT	135	4.6802597E-9	11.267423	18
CGCATAT	110	4.9687515E-7	11.2279005	8
GAACAAA	1115	0.0	11.068799	1
CGCGCCA	95	1.3604802E-5	11.000399	10
GCTAGAT	95	1.3714916E-5	10.992401	3
ACCAGTT	390	0.0	10.947297	12
GTTCGCG	70	0.0014824877	10.865442	16
TCACGCA	175	2.0008883E-11	10.863946	4
CATGCGA	70	0.0014912721	10.85775	6
GTATCAA	6220	0.0	10.821509	1
>>END_MODULE