FastQCFastQC Report
Thu 26 May 2016
SRR1364317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364317_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553913
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT237440.9297106048639872No Hit
GTACTTTTTTTTTTTTTTTTTTTTT219230.8584082543140662No Hit
TATCAACGCAGAGTACTTTTTTTTT182780.7156860864093647No Hit
GGTATCAACGCAGAGTACTTTTTTT149870.586825001478124No Hit
GAGTACTTTTTTTTTTTTTTTTTTT110640.4332175763230776No Hit
ACGCAGAGTACTTTTTTTTTTTTTT81760.3201361988446748No Hit
GTATAACGCAGAGTACTTTTTTTTT31180.12208716585099023No Hit
GCAGAGTACTTTTTTTTTTTTTTTT30810.12063840859105224No Hit
ATCAACGCAGAGTACTTTTTTTTTT29830.11680115963229759No Hit
GTGGTATCAACGCAGAGTACTTTTT29020.113629555901082No Hit
GTGGTATCAACGCAGAGTACATGGG27120.10618999159329233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA4400.015.5467694
TATAACG4550.014.8239772
ATAACGC4850.014.1040083
TATCACG1700.013.4115862
GTCGGTT655.468902E-513.15032112
GGTATCA43750.013.0531791
CGCGCCA1102.748493E-912.95462510
ACAACGC1650.012.667493
GTATAAC6100.012.3060061
AGCCGTC550.00306788212.0916957
CGGTTAC550.003068782412.091229
TGAACCG951.0380936E-612.0024265
TACAACG1850.011.8106522
TCTTCGC1459.822543E-1111.79109814
TATAAGC5600.011.7061183
GCGTTAT658.0116105E-411.69491
TATTTCG658.012927E-411.694675
GGACCGA1302.6157068E-911.6930686
TGGTATC13500.011.5405862
GTGGTAT14500.011.4025271
Produced by FastQC (version 0.11.5)