##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364317_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2553913
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.84058110045252	31.0	30.0	34.0	26.0	34.0
2	29.84482674233617	31.0	30.0	34.0	26.0	34.0
3	29.893644380211857	31.0	30.0	34.0	26.0	34.0
4	33.273862108850224	35.0	33.0	37.0	27.0	37.0
5	32.89161377071184	35.0	33.0	37.0	26.0	37.0
6	32.79676089201159	35.0	33.0	37.0	26.0	37.0
7	32.53015431614154	35.0	33.0	37.0	25.0	37.0
8	32.418291069429536	35.0	33.0	37.0	23.0	37.0
9	33.216642461978935	37.0	33.0	39.0	17.0	39.0
10	33.32631103722014	37.0	33.0	39.0	19.0	39.0
11	33.2693415946432	37.0	33.0	39.0	18.0	39.0
12	33.20293095340366	37.0	33.0	39.0	17.0	39.0
13	33.26142785599979	37.0	33.0	39.0	18.0	39.0
14	34.20356370792584	38.0	33.0	41.0	18.0	41.0
15	34.24555574132713	38.0	33.0	41.0	18.0	41.0
16	34.20253900583145	38.0	33.0	41.0	18.0	41.0
17	34.170940043768134	38.0	33.0	41.0	18.0	41.0
18	34.11882550423605	38.0	33.0	41.0	17.0	41.0
19	34.02713365725457	39.0	33.0	41.0	18.0	41.0
20	33.93144245712364	39.0	33.0	41.0	18.0	41.0
21	33.86860398141988	39.0	33.0	41.0	17.0	41.0
22	33.806357146856605	39.0	33.0	41.0	16.0	41.0
23	33.53997414947181	39.0	33.0	41.0	10.0	41.0
24	33.45929716478204	38.0	33.0	41.0	2.0	41.0
25	33.29919813243443	38.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.14125073082921347
1101	2	0.3028180393713953
1101	3	-1.7417532648105443
1101	4	-0.6204603787946041
1101	5	0.18589210757595964
1101	6	0.4352723307302213
1101	7	0.7302461226356485
1101	8	0.8103297780933545
1101	9	1.6138242473561633
1101	10	1.2878290692567518
1101	11	1.1564138348627324
1101	12	1.468881292926369
1101	13	1.2123423604273427
1101	14	1.263107633221459
1101	15	1.1830542947233482
1101	16	1.2715414200813626
1101	17	1.2184341250773585
1101	18	1.091263367270507
1101	19	1.1613596492017493
1101	20	1.3008371294625434
1101	21	1.3256442280287928
1101	22	1.4291350744658473
1101	23	1.646215421177395
1101	24	1.6303992309954864
1101	25	1.7427788335915366
1102	1	0.5945429633652353
1102	2	0.5839974665335355
1102	3	0.7014930497047374
1102	4	0.6562521801597754
1102	5	0.7570884567793712
1102	6	0.8591411581480486
1102	7	0.9072461452807161
1102	8	0.9367427788324036
1102	9	1.5704271630115159
1102	10	1.2228121214824483
1102	11	1.3047329790027007
1102	12	1.3912304561911313
1102	13	1.2225622337384863
1102	14	1.268903355156496
1102	15	1.262626874222974
1102	16	1.1901645691044322
1102	17	1.2620343717027254
1102	18	1.4078122023876531
1102	19	1.2955009225133054
1102	20	1.5361925901146805
1102	21	1.721940884587525
1102	22	1.6440534904639748
1102	23	1.8517412808150269
1102	24	1.9144230587529876
1102	25	1.5632901695064092
1103	1	-2.4788202107591424
1103	2	-2.35499628407311
1103	3	-2.5567984094576808
1103	4	-2.545645410426566
1103	5	-4.532821423533921
1103	6	-9.16180401593153
1103	7	-10.581451561781769
1103	8	-11.570098328288317
1103	9	-12.867668750616936
1103	10	-10.87653046025618
1103	11	-13.420159317892015
1103	12	-12.855680312626113
1103	13	-13.190635036538445
1103	14	-12.380721830570693
1103	15	-13.759443862637994
1103	16	-7.710899769034789
1103	17	-5.60062643631322
1103	18	-5.054439445824514
1103	19	-4.674273143624241
1103	20	-10.37057049291937
1103	21	-6.780407304432359
1103	22	-12.42407572339669
1103	23	-13.392334115091877
1103	24	-15.054766760037008
1103	25	-15.199339279546479
1104	1	0.14330948966228618
1104	2	0.3429403504971731
1104	3	0.3792701342380944
1104	4	0.5141179479855325
1104	5	0.38607518473874336
1104	6	0.5637081781094011
1104	7	0.46705036067720584
1104	8	0.7360998936679763
1104	9	0.9626876337566657
1104	10	0.8777539824153919
1104	11	0.7425534730161019
1104	12	0.7597374020669392
1104	13	0.6601195424596611
1104	14	0.778507080552977
1104	15	0.7978550178666808
1104	16	0.4437473738698401
1104	17	0.41090000719307795
1104	18	0.49466266795698743
1104	19	0.6427580700180116
1104	20	0.9882626612799328
1104	21	0.8257416094983583
1104	22	0.4964210872789607
1104	23	0.9167468719550698
1104	24	1.0549131256126927
1104	25	1.1569536803016476
1105	1	0.3084286593939112
1105	2	0.27994162181728655
1105	3	-0.025194976242080003
1105	4	0.22385251976977827
1105	5	0.31473089122814457
1105	6	0.6069039708521089
1105	7	0.6119003807954186
1105	8	0.4662371697937928
1105	9	0.5254572225705587
1105	10	0.2428478390867994
1105	11	0.7678206296982779
1105	12	0.989027446289235
1105	13	0.7441167769133799
1105	14	0.6307532368403415
1105	15	0.34908600709729853
1105	16	0.7055352315348813
1105	17	0.6677359502273603
1105	18	0.7217274297214189
1105	19	0.7278099523462842
1105	20	1.0270179009889517
1105	21	0.9852985176025015
1105	22	0.8099545071300653
1105	23	1.251145053948413
1105	24	1.130566117559745
1105	25	1.342013118767298
1106	1	0.0421514614918479
1106	2	0.211558696127927
1106	3	0.07463625395992679
1106	4	-1.3739541219708826
1106	5	-0.17387000714646916
1106	6	0.14874654757745276
1106	7	0.4427755171094887
1106	8	0.5163241511512169
1106	9	1.0968111458192524
1106	10	0.6475785863475352
1106	11	0.7184067801890066
1106	12	0.939019656913942
1106	13	0.8119654812806232
1106	14	0.10105169973694927
1106	15	0.7072291498505834
1106	16	0.362935138062376
1106	17	0.3525947028887302
1106	18	0.4892781904837378
1106	19	-0.8686647062194268
1106	20	0.41727598895695905
1106	21	0.044486085759537275
1106	22	0.5100622601549389
1106	23	0.4898011270646876
1106	24	0.640062664270026
1106	25	0.9430685791193767
1107	1	0.07947725323176513
1107	2	-0.059894079738974426
1107	3	-0.18662772728800547
1107	4	0.011746469126975967
1107	5	0.07137668703681754
1107	6	0.0029909232303353406
1107	7	-0.06805692448138245
1107	8	-0.1545012201958187
1107	9	0.6885520181981661
1107	10	0.5245996569480624
1107	11	0.4002219219760619
1107	12	0.31679998252733554
1107	13	0.1833797211338677
1107	14	0.3487869923558691
1107	15	0.09904645729790218
1107	16	0.0780552685115623
1107	17	-0.036969999396681885
1107	18	0.048945315760818175
1107	19	0.36972130361521494
1107	20	0.3373580292637186
1107	21	0.40524495859706633
1107	22	0.3980524000311121
1107	23	0.5522715084756769
1107	24	0.8592306685503956
1107	25	0.4327670427745005
1108	1	-0.12278099036213419
1108	2	-0.05917283592151534
1108	3	0.09921228155406325
1108	4	-0.052845326339955534
1108	5	0.074351464540797
1108	6	0.1039096691641177
1108	7	-0.18743072031505648
1108	8	0.30004792234984023
1108	9	0.6776066330282191
1108	10	0.44573897726446887
1108	11	0.5233112067483674
1108	12	0.4665778632950861
1108	13	0.029416616664356354
1108	14	0.01978193831125452
1108	15	-0.1700380246248443
1108	16	-0.805992714768891
1108	17	-0.8607280910175632
1108	18	-0.7918227005769083
1108	19	-0.7778160428104002
1108	20	-0.05832763277341968
1108	21	-0.25732477943160603
1108	22	-0.13716395427449157
1108	23	-0.554148283685528
1108	24	-0.17482667174606803
1108	25	-0.39275349618961997
1109	1	0.0024071297270822356
1109	2	-0.011238807583794141
1109	3	-0.031416993479616906
1109	4	0.0700980056619187
1109	5	0.05923003311624342
1109	6	0.30880624963337056
1109	7	0.3471946645465138
1109	8	0.27566698816271895
1109	9	1.0023148701203155
1109	10	0.48740847637999707
1109	11	0.4196772575538148
1109	12	0.5500340256700795
1109	13	0.5592619029318939
1109	14	0.06796046694297075
1109	15	-0.13649081038998645
1109	16	0.22018494303985392
1109	17	0.15587925962140758
1109	18	0.11840134406082825
1109	19	0.33810792268416634
1109	20	0.6405453213508281
1109	21	0.523105955409001
1109	22	0.16438609819817174
1109	23	0.5118978079071823
1109	24	0.4290048872751342
1109	25	0.3293824018124525
1110	1	-0.1940422931184287
1110	2	-0.05322861638744314
1110	3	-0.15397697446397274
1110	4	-0.04244994198563745
1110	5	-0.10841574110270358
1110	6	0.030878954757191934
1110	7	0.009087909536340533
1110	8	-0.07749807653503638
1110	9	-3.5587746668190583
1110	10	-1.522689424471249
1110	11	-0.6366491578488578
1110	12	-0.1269552631745725
1110	13	-0.4434187085398875
1110	14	-0.03636470055811003
1110	15	0.021311397207355753
1110	16	-0.29040885013530726
1110	17	-0.12026858700554044
1110	18	-0.10202621079484686
1110	19	-0.37749738386879983
1110	20	0.14488356210670617
1110	21	-0.1762281999547497
1110	22	0.21301652619870737
1110	23	0.27242222369611113
1110	24	0.21394390351271397
1110	25	0.3371883234026356
1111	1	-0.04154701789990867
1111	2	0.08016613910511694
1111	3	-0.00683133307488859
1111	4	-0.05898313357552354
1111	5	6.948187838986541E-4
1111	6	0.04186879647701147
1111	7	0.17968027901424932
1111	8	0.3478267521916365
1111	9	0.9466310737904351
1111	10	0.3278556971918718
1111	11	0.567216303998535
1111	12	0.6146299010115825
1111	13	0.4314932924050723
1111	14	0.2605204872519593
1111	15	0.3574642438881952
1111	16	0.20354638256017665
1111	17	0.2567813775585961
1111	18	0.15972136775464918
1111	19	0.4316604238325894
1111	20	0.6475631038029022
1111	21	0.5602423882805496
1111	22	0.4284996351049202
1111	23	0.8521310590374753
1111	24	0.7772825522252091
1111	25	0.8478805038892858
1112	1	-0.2033671839225235
1112	2	-0.2137081989512737
1112	3	-0.30526592589011514
1112	4	-0.18436240937343484
1112	5	-0.20846147146942684
1112	6	-0.2557267380871764
1112	7	-0.023513151642681862
1112	8	-0.3552074541916497
1112	9	0.2442570219799336
1112	10	-0.03325958833839593
1112	11	0.1813586397649516
1112	12	-0.5716660772337292
1112	13	-0.324351490763668
1112	14	-1.1761225641291801
1112	15	-0.46541038041407745
1112	16	-0.4139629204310893
1112	17	-0.47597051882038954
1112	18	-0.7411391589377772
1112	19	-0.39499980230165477
1112	20	-0.36218177955190356
1112	21	-0.5128218739206858
1112	22	-0.21573966645512144
1112	23	-0.02952952490413452
1112	24	-0.1504743534567723
1112	25	-0.22087575773945645
1113	1	-1.9458604832405548
1113	2	-1.8905246694396816
1113	3	-2.053881794654515
1113	4	-2.062327541846795
1113	5	-1.677067969424634
1113	6	-1.742391412534566
1113	7	-2.009045397002936
1113	8	-2.3573212357374267
1113	9	-11.597365603728182
1113	10	-4.701445875597969
1113	11	-3.0937926674444824
1113	12	-13.341580983645542
1113	13	-5.102613242551595
1113	14	-4.128594483982937
1113	15	-3.350834898361576
1113	16	-3.081581425196738
1113	17	-3.2561066169113673
1113	18	-3.3909986890322834
1113	19	-4.170954822417539
1113	20	-15.314501331523921
1113	21	-17.660959056664375
1113	22	-12.279820690750974
1113	23	-18.56518951210228
1113	24	-19.62202712236487
1113	25	-16.10440360914941
1114	1	0.10219847930592607
1114	2	0.0783027125030955
1114	3	0.13872842508855499
1114	4	-0.23693696654863317
1114	5	0.04797123679897197
1114	6	0.21176384486379618
1114	7	0.17339510854089468
1114	8	0.34783064650009976
1114	9	1.0228920995512851
1114	10	0.23496889500327
1114	11	0.1969870752663212
1114	12	0.25171345339976625
1114	13	0.23888444648338947
1114	14	0.0991281746168724
1114	15	0.13050930103717207
1114	16	0.16544714584463804
1114	17	0.21939090498426594
1114	18	0.1463972347250717
1114	19	-0.07999280746985704
1114	20	-0.40737999061460783
1114	21	-0.5037654392794764
1114	22	-0.7241913320465869
1114	23	-0.23579926204826052
1114	24	0.2731134516157496
1114	25	-0.1805158198835244
1115	1	-0.46025738648350156
1115	2	-0.37895616778595453
1115	3	-0.44268120424423074
1115	4	-0.30421046029372434
1115	5	-0.7649471416349343
1115	6	-0.6014904857260817
1115	7	-0.9107243497524493
1115	8	-0.639947127164394
1115	9	-0.022587307877230955
1115	10	-0.4760039687621145
1115	11	-0.49554775316207866
1115	12	-0.6015351484191882
1115	13	-0.6087263136106813
1115	14	-0.8268033984999192
1115	15	-0.705421105964696
1115	16	-0.562376309909169
1115	17	-0.7210796223865827
1115	18	-0.8223832774585134
1115	19	-0.7744873568080877
1115	20	-1.182810550784751
1115	21	-0.5584462801038654
1115	22	-1.067932395302769
1115	23	-0.9145239580485764
1115	24	-0.4229282823416085
1115	25	-0.39297617428990606
1116	1	-0.6422028856331572
1116	2	-0.47431206882617616
1116	3	-0.4599720028749701
1116	4	-0.38217138283471996
1116	5	-1.3429223765919112
1116	6	-1.0817649169683001
1116	7	-1.9947586630437648
1116	8	-1.256063395009022
1116	9	-0.21986337509083143
1116	10	-1.3855868365097876
1116	11	-1.0176592431488842
1116	12	-1.9960562207551753
1116	13	-1.6143380770890872
1116	14	-1.1851580846441934
1116	15	-2.2813967512109343
1116	16	-2.592668590186097
1116	17	-3.3617515363859702
1116	18	-2.4360919998804107
1116	19	-1.9846157661448451
1116	20	-2.168063109958812
1116	21	-1.5156272651673035
1116	22	-1.4078689207750301
1116	23	-1.486478073687504
1116	24	-1.9549780339589624
1116	25	-1.4578168117607682
1201	1	0.8890902370020513
1201	2	0.9505729136401229
1201	3	0.5584435043665152
1201	4	1.0164982414937853
1201	5	1.1249462931525684
1201	6	1.1830200641820383
1201	7	1.349720981670636
1201	8	1.269672611357926
1201	9	2.181711597002291
1201	10	1.761402624960411
1201	11	1.7699262440156716
1201	12	1.8440089019692891
1201	13	1.7358565415607785
1201	14	1.7513907428091926
1201	15	1.8222696776298193
1201	16	1.6741806131161567
1201	17	1.5848529789497974
1201	18	1.652369056650258
1201	19	1.71554191987574
1201	20	1.9129425332096233
1201	21	2.065005733207208
1201	22	2.012702467908994
1201	23	2.2085042381291657
1201	24	2.196865822551338
1201	25	2.335213534788018
1202	1	0.8386798913169002
1202	2	0.8483215584476866
1202	3	0.874362436400073
1202	4	0.9893731674129143
1202	5	0.9684665905117384
1202	6	1.02906054957716
1202	7	1.144534585571492
1202	8	1.0666343846701452
1202	9	1.923433559271075
1202	10	1.4685988422489515
1202	11	1.591369706966283
1202	12	1.6053860293768807
1202	13	1.5330998701269323
1202	14	1.5164068631900491
1202	15	1.5516022497495783
1202	16	1.4459307884488695
1202	17	1.5817918007778715
1202	18	1.5620729313594452
1202	19	1.5594299524348827
1202	20	1.8375161566480145
1202	21	1.9779026132757949
1202	22	1.8243430259305384
1202	23	2.1143036114705325
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2211	7	0.2664480614410252
2211	8	0.15124786959257364
2211	9	0.7244543160580577
2211	10	0.09829728386838354
2211	11	0.38295898182280297
2211	12	0.4977061719662643
2211	13	0.33445395365701813
2211	14	0.33103146709717635
2211	15	0.29960538207289744
2211	16	0.28598991087805814
2211	17	0.13950658061052934
2211	18	0.21472553818153273
2211	19	0.02472315799180791
2211	20	0.5115842841020779
2211	21	0.685724380240508
2211	22	0.43749681835758025
2211	23	0.859563648530731
2211	24	0.8718900535241971
2211	25	1.038268011751562
2212	1	-0.16966880195292688
2212	2	-0.03205274424948357
2212	3	0.009868779440378006
2212	4	0.03860705015367216
2212	5	-0.1632574171139538
2212	6	-0.076071899745493
2212	7	-0.04592328991507344
2212	8	-0.10154275068299512
2212	9	0.5694615530780212
2212	10	0.09250034791430295
2212	11	0.1624489841944694
2212	12	-0.10432686293962234
2212	13	-0.40270362989078734
2212	14	-0.23338207727280036
2212	15	-0.08547988267775253
2212	16	-0.09242896699181813
2212	17	-0.304966005089355
2212	18	-0.22425429738894564
2212	19	0.015085347335933363
2212	20	0.2856654721120151
2212	21	0.38330527333659603
2212	22	0.01914989100853859
2212	23	0.4527346279667981
2212	24	0.46775185751205584
2212	25	0.5566141583027644
2213	1	-0.08166447997907866
2213	2	-0.09720622493484044
2213	3	-0.19960325316368
2213	4	-0.09962316317226794
2213	5	-0.11857543648801538
2213	6	0.007915781308682313
2213	7	-0.0645021109228594
2213	8	-0.06668027703514667
2213	9	0.6573136487071949
2213	10	0.03532887514229799
2213	11	0.33970058072809195
2213	12	0.3317035229131733
2213	13	-0.18535835391383415
2213	14	-0.11681025636624298
2213	15	0.04447404780976427
2213	16	-0.1867375612060087
2213	17	-0.11505547038812836
2213	18	-0.35793494005149284
2213	19	-0.16662388989289667
2213	20	0.2366493043208422
2213	21	-0.020001784860404825
2213	22	0.12030966100206797
2213	23	-0.17585636234617397
2213	24	-0.02226887211811146
2213	25	0.28959341546901385
2214	1	-0.888525840614026
2214	2	-0.6185253125275132
2214	3	-0.6844010407916556
2214	4	-0.5205362024815372
2214	5	-0.7837238759289136
2214	6	-0.6859150960127636
2214	7	-0.7364983996644305
2214	8	-0.775829030287678
2214	9	-10.601344446979201
2214	10	-3.9115588870756532
2214	11	-1.7371640378818114
2214	12	-1.1047313154084577
2214	13	-0.9169781994487707
2214	14	-0.9488591412453218
2214	15	-0.8981888073530868
2214	16	-1.1109361429517577
2214	17	-0.9823189784535202
2214	18	-1.3663250972624041
2214	19	-1.4713334071024136
2214	20	-1.1237760520950815
2214	21	-1.7146342983608456
2214	22	-1.4858536321895244
2214	23	-1.911899317492903
2214	24	-2.1999573007499365
2214	25	-9.644400011920641
2215	1	-0.2977256833959565
2215	2	-0.07663807511788434
2215	3	0.08897273482092771
2215	4	-0.1837051119191031
2215	5	-0.1303338613448659
2215	6	-0.093893568170337
2215	7	-0.15632718367231746
2215	8	-0.17034708450722036
2215	9	0.7524805031144055
2215	10	0.6122755098930739
2215	11	0.7596914283766409
2215	12	0.6706878465778701
2215	13	0.45762366992921244
2215	14	0.2334572739069145
2215	15	-0.08382197209073183
2215	16	0.2851201314627261
2215	17	0.11479644925891819
2215	18	0.20575955915060717
2215	19	0.2727417817488913
2215	20	0.3826633379264024
2215	21	0.39073697417460806
2215	22	0.19283969948888569
2215	23	0.6566708600324986
2215	24	0.7459795086197119
2215	25	0.8638448000555101
2216	1	-0.6259581973300037
2216	2	-0.656708885741498
2216	3	-0.6326348725578121
2216	4	-0.5031212100721731
2216	5	-1.4851654703644215
2216	6	-1.7305291557203084
2216	7	-1.1597946402618646
2216	8	-2.0171273754382426
2216	9	-0.5006084674416869
2216	10	-1.164319662476661
2216	11	-0.5498980986447037
2216	12	-1.7137312384430743
2216	13	-1.3109668646696981
2216	14	-1.5297359960577452
2216	15	-1.254334805535585
2216	16	-2.348438309717274
2216	17	-2.6546783775531786
2216	18	-2.103676533645153
2216	19	-3.2920378344750105
2216	20	-2.1909852024310617
2216	21	-2.4400623968459385
2216	22	-2.1788266925876805
2216	23	-1.7025987975368295
2216	24	-2.0585140743776726
2216	25	-2.206915444677122
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79899.0
3	4.0
4	1089.0
5	11666.0
6	15765.0
7	12746.0
8	10196.0
9	8253.0
10	7082.0
11	6651.0
12	5745.0
13	5505.0
14	5545.0
15	5689.0
16	5867.0
17	6286.0
18	6898.0
19	8958.0
20	11712.0
21	15622.0
22	19117.0
23	22551.0
24	26498.0
25	30634.0
26	33787.0
27	37768.0
28	42141.0
29	49507.0
30	60843.0
31	74665.0
32	92688.0
33	115225.0
34	143225.0
35	180242.0
36	236451.0
37	351300.0
38	806093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.958583362610305	18.411515583771116	12.174834648740934	24.45506640487764
2	16.118568788903843	21.3832372773644	38.55670539055473	23.941488543177027
3	18.613591405798484	27.259243480971122	29.3235699954736	24.80359511775679
4	12.023000973057849	16.740823986169655	36.3848845154583	34.85129052531419
5	12.524328010318328	39.147797974797186	34.09528785681245	14.232586158072042
6	30.966526970541636	36.94051388646316	18.313441450980914	13.779517692014295
7	27.751982429921767	32.40881292315597	22.305469658091543	17.53373498883072
8	27.068607964281167	34.48304628369447	20.331926643232713	18.11641910879165
9	27.173197372755318	14.897028819553382	19.901821046080187	38.02795276161111
10	14.967373588568398	30.55041670697883	32.54721027437208	21.934999430080694
11	34.75232449781955	22.797960137364434	23.390928886446368	19.058786478369647
12	23.115909652474272	26.700653434043183	30.346993173538877	19.836443739943668
13	28.611418489203395	21.608249166555073	27.01869093739746	22.761641406844078
14	21.82210897819191	20.5430622955724	29.327932705165722	28.306896021069967
15	23.518963539772	28.924934087617167	25.024777103958897	22.53132526865194
16	22.433471094121398	28.172981812286636	26.643006897437527	22.750540196154446
17	22.15062580619392	27.748303811070045	27.93736534065144	22.163705042084594
18	21.306353034794075	26.221634286470653	30.66988256035577	21.802130118379505
19	22.87810782878946	25.750858328004067	30.11426228436063	21.25677155884584
20	22.731656999728614	25.51323667570115	30.26846165865625	21.48664466591399
21	23.257856640492225	24.773851872389017	30.159545056538988	21.80874643057977
22	22.813610787065265	25.136676859915468	30.015691259298944	22.034021093720323
23	22.12432974939588	25.167707048845422	30.61676173753548	22.091201464223214
24	22.301664156016145	25.326661933826117	30.28619849658566	22.085475413572077
25	22.173198963612773	25.226464720108815	30.536263753493497	22.06407256278492
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2288.0
1	2288.0
2	3369.5
3	4451.0
4	4451.0
5	4451.0
6	15039.0
7	25627.0
8	25627.0
9	25627.0
10	22288.5
11	18950.0
12	18950.0
13	18950.0
14	16894.5
15	14839.0
16	14839.0
17	14839.0
18	25428.0
19	36017.0
20	36017.0
21	36017.0
22	52249.5
23	68482.0
24	68482.0
25	68482.0
26	97530.0
27	126578.0
28	126578.0
29	126578.0
30	147146.5
31	167715.0
32	167715.0
33	167715.0
34	188827.5
35	209940.0
36	209940.0
37	209940.0
38	226222.0
39	242504.0
40	242504.0
41	242504.0
42	261778.0
43	281052.0
44	281052.0
45	281052.0
46	301415.0
47	321778.0
48	321778.0
49	321778.0
50	314303.0
51	306828.0
52	306828.0
53	306828.0
54	284353.5
55	261879.0
56	261879.0
57	261879.0
58	229888.5
59	197898.0
60	197898.0
61	197898.0
62	163328.5
63	128759.0
64	128759.0
65	128759.0
66	100467.0
67	72175.0
68	72175.0
69	72175.0
70	54144.0
71	36113.0
72	36113.0
73	36113.0
74	25825.5
75	15538.0
76	15538.0
77	15538.0
78	12241.0
79	8944.0
80	8944.0
81	8944.0
82	6210.5
83	3477.0
84	3477.0
85	3477.0
86	2398.5
87	1320.0
88	1320.0
89	1320.0
90	1010.0
91	700.0
92	700.0
93	700.0
94	378.0
95	56.0
96	56.0
97	56.0
98	30.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02787878835340123
2	7.83112032398911E-4
3	1.9577800809972775E-4
4	0.004228804974954119
5	0.030776302873277202
6	0.010767790445485027
7	0.01883384437919381
8	0.02498127383352526
9	0.032107593328355354
10	0.03602315349034991
11	0.03899897921346576
12	0.006813074681870525
13	0.0021535580890970052
14	0.005325161820312595
15	0.005638406633272159
16	0.00826183194180851
17	0.009671433600126551
18	0.01354783816050116
19	0.015740551851218112
20	0.014252638989660178
21	0.010846101648724918
22	0.010258767624425734
23	0.007987742730468893
24	0.0049336058041131396
25	0.10740381524351064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2553913.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.77399078577529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29771809765309	32.08533636625021
2	13.571812678735535	11.881848062904751
3	5.120804246459822	6.724741137008737
4	2.4509663638303705	4.291543161062232
5	1.4135858665166774	3.0939147347901605
6	0.8440927002018521	2.216958364858564
7	0.5961729907919724	1.8267809703959126
8	0.4228714680332436	1.480861739620358
9	0.2993451876543843	1.1793180137534272
>10	1.7077577934660155	13.871832229280917
>50	0.1582684972649979	4.819367623424591
>100	0.10634571594971272	8.745368104649785
>500	0.007378844406061127	2.179472094067745
>1k	0.0023396335921655577	1.7356208098465171
>5k	8.9985907390983E-5	0.3220560968931307
>10k+	4.49929536954915E-4	3.5449804911930274
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	23744	0.9297106048639872	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	21923	0.8584082543140662	No Hit
TATCAACGCAGAGTACTTTTTTTTT	18278	0.7156860864093647	No Hit
GGTATCAACGCAGAGTACTTTTTTT	14987	0.586825001478124	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	11064	0.4332175763230776	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	8176	0.3201361988446748	No Hit
GTATAACGCAGAGTACTTTTTTTTT	3118	0.12208716585099023	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	3081	0.12063840859105224	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2983	0.11680115963229759	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2902	0.113629555901082	No Hit
GTGGTATCAACGCAGAGTACATGGG	2712	0.10618999159329233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.915560161994555E-5	0.0	0.0	3.915560161994555E-5	0.0
8	3.915560161994555E-5	0.0	0.0	3.915560161994555E-5	0.0
9	3.915560161994555E-5	0.0	0.0	3.915560161994555E-5	0.0
10	3.915560161994555E-5	0.0	0.0	3.915560161994555E-5	0.0
11	3.915560161994555E-5	0.0	0.0	3.915560161994555E-5	0.0
12	7.83112032398911E-5	0.0	0.0	7.83112032398911E-5	0.0
13	7.83112032398911E-5	0.0	0.0	7.83112032398911E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	440	0.0	15.546769	4
TATAACG	455	0.0	14.823977	2
ATAACGC	485	0.0	14.104008	3
TATCACG	170	0.0	13.411586	2
GTCGGTT	65	5.468902E-5	13.150321	12
GGTATCA	4375	0.0	13.053179	1
CGCGCCA	110	2.748493E-9	12.954625	10
ACAACGC	165	0.0	12.66749	3
GTATAAC	610	0.0	12.306006	1
AGCCGTC	55	0.003067882	12.091695	7
CGGTTAC	55	0.0030687824	12.09122	9
TGAACCG	95	1.0380936E-6	12.002426	5
TACAACG	185	0.0	11.810652	2
TCTTCGC	145	9.822543E-11	11.791098	14
TATAAGC	560	0.0	11.706118	3
GCGTTAT	65	8.0116105E-4	11.6949	1
TATTTCG	65	8.012927E-4	11.69467	5
GGACCGA	130	2.6157068E-9	11.693068	6
TGGTATC	1350	0.0	11.540586	2
GTGGTAT	1450	0.0	11.402527	1
>>END_MODULE