FastQCFastQC Report
Thu 26 May 2016
SRR1364316_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364316_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604854
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA94160.5867200380844613No Hit
GTATCAACGCAGAGTACTTTTTTTT82700.5153116732113949No Hit
GTACTTTTTTTTTTTTTTTTTTTTT56460.35180770337987133No Hit
TATCAACGCAGAGTACTTTTTTTTT52260.3256370984525695No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA46210.2879389651644324No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN45380.28276715514308465No Hit
GAACAAAAAAAAAAAAAAAAAAAAA37430.23322993867354913No Hit
GGTATCAACGCAGAGTACTTTTTTT34310.21378891787041063No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26500.16512405489845183No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21810.1359002127296315No Hit
AAACAAAAAAAAAAAAAAAAAAAAA20130.12543197075871076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2200.015.5354792
TATCACG1400.014.2408542
TAACGCA2550.014.1646054
ATAACGC2550.014.1477783
CGCATAT604.0883909E-412.6680438
TGACCGA853.9167335E-612.30161219
GGTATCA20950.012.23557951
ATCACGC1650.012.083153
GTTATCA3500.011.9351941
CGAAAGG802.8477454E-511.8826118
CTCGAAC657.9806324E-411.699800518
CACGCCC907.405213E-611.618916516
GAACAAA12150.011.5645911
CCAATCG752.0609806E-411.40766315
TCACGCA1850.011.3034764
GTATAAC3700.011.2900491
CGAAACC1104.926951E-711.23446818
GTATCAA51700.011.2017051
CGGTGAG855.286534E-511.18398415
CGCGCCA600.005873768611.08384410
Produced by FastQC (version 0.11.5)