##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364316_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1604854
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.77976189734393	30.0	26.0	31.0	2.0	34.0
2	25.692114049003834	30.0	25.0	31.0	2.0	34.0
3	25.24274606911283	30.0	23.0	31.0	2.0	34.0
4	28.835470391699182	35.0	27.0	37.0	2.0	37.0
5	29.517103113429634	35.0	30.0	37.0	2.0	37.0
6	29.599688819045223	35.0	30.0	37.0	2.0	37.0
7	29.2079404107788	35.0	29.0	37.0	2.0	37.0
8	28.692420618947267	35.0	27.0	37.0	2.0	37.0
9	29.658530931785695	35.0	27.0	39.0	2.0	39.0
10	29.07734036865659	35.0	26.0	39.0	2.0	39.0
11	29.669186729758596	35.0	27.0	39.0	2.0	39.0
12	29.223401630304064	35.0	27.0	39.0	2.0	39.0
13	28.806540034171334	35.0	25.0	39.0	2.0	39.0
14	29.08591747286669	36.0	23.0	40.0	2.0	41.0
15	29.80489627093804	36.0	27.0	40.0	2.0	41.0
16	30.14422558064472	36.0	27.0	40.0	2.0	41.0
17	30.3040637964575	37.0	27.0	40.0	2.0	41.0
18	30.14188081906516	37.0	27.0	40.0	2.0	41.0
19	30.132216388531294	37.0	27.0	40.0	2.0	41.0
20	29.990807886574107	37.0	27.0	40.0	2.0	41.0
21	29.914645195139247	37.0	27.0	40.0	2.0	41.0
22	29.975286848523293	37.0	27.0	40.0	2.0	41.0
23	29.97128025353085	37.0	27.0	40.0	2.0	41.0
24	29.84448118021951	37.0	27.0	40.0	2.0	41.0
25	29.657104010707517	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5646158795853857
1101	2	0.6986857161710809
1101	3	1.4442212792774995
1101	4	0.6144350664956839
1101	5	0.46574920975995937
1101	6	0.20927349232970371
1101	7	0.3820219155179636
1101	8	0.6665351650610383
1101	9	0.4974001421303065
1101	10	0.8773426138542142
1101	11	0.2623792541356771
1101	12	0.5199770636564374
1101	13	0.43858994648228844
1101	14	0.7676592500968269
1101	15	-0.019526795473456815
1101	16	-0.377552260735019
1101	17	-0.9977447491761708
1101	18	-6.773532040731592
1101	19	-8.093519232074392
1101	20	-8.97443549165408
1101	21	-10.559681380431886
1101	22	-11.010710352925717
1101	23	-12.004119447559326
1101	24	-12.028903986604721
1101	25	-12.328082557353685
1102	1	1.4433953861250792
1102	2	1.6545068115316326
1102	3	2.374827739175398
1102	4	1.820169702293228
1102	5	1.0659436069226693
1102	6	1.0560423519457807
1102	7	1.299244048650877
1102	8	1.6085909963641
1102	9	1.821072223065471
1102	10	-1.8633804521403512
1102	11	0.36902301948345695
1102	12	1.4781043913829386
1102	13	1.7394965965590252
1102	14	2.3671381420491002
1102	15	1.862749573727232
1102	16	1.4943283522938735
1102	17	1.2349783116662465
1102	18	1.513428858937317
1102	19	1.1631247908810245
1102	20	1.5853975850376436
1102	21	1.6350996176560209
1102	22	1.491486879209031
1102	23	1.4836301822907032
1102	24	1.628416675008971
1102	25	1.6649821620568588
1103	1	-0.9735748330855536
1103	2	-1.5047016017698418
1103	3	-3.17898592525869
1103	4	-0.8489678168012063
1103	5	-4.630311272766075
1103	6	-7.031100076213452
1103	7	-8.282359361582433
1103	8	-3.098761503211577
1103	9	-7.973563056920948
1103	10	-5.112280482169208
1103	11	-9.27599676039199
1103	12	-3.6906740870680927
1103	13	-1.0253317820134313
1103	14	-0.775645793819713
1103	15	-1.7200419346351516
1103	16	-1.6937142886785388
1103	17	-1.362863527666331
1103	18	-0.7928034338309509
1103	19	-6.845214744063419
1103	20	-2.357825033217285
1103	21	-6.606710991668589
1103	22	-2.8769965331132425
1103	23	-2.253535746710643
1103	24	-6.3760484849925945
1103	25	-4.106906261167197
1104	1	1.4940829170896777
1104	2	1.513737767718478
1104	3	2.3174654089548845
1104	4	1.78160580538081
1104	5	1.5253540409411954
1104	6	1.326465900932945
1104	7	1.4235235666781172
1104	8	1.6328667752698465
1104	9	1.7190119671226078
1104	10	2.033206664716541
1104	11	1.5811209470753695
1104	12	1.9117840608445995
1104	13	2.0761923348953033
1104	14	2.6676228256726837
1104	15	1.8037261387832721
1104	16	0.869631922220119
1104	17	0.6332536418774133
1104	18	0.5821814880071194
1104	19	1.174802534695118
1104	20	-9.335802766439777
1104	21	-3.7397117904036534
1104	22	-1.1243338112197527
1104	23	-0.018952148490679832
1104	24	0.5598636215488817
1104	25	0.8924156290637839
1105	1	1.0543033409055589
1105	2	1.1405316152536997
1105	3	1.7313546837398093
1105	4	1.2917747022649415
1105	5	0.9922596230385494
1105	6	0.8715417236175078
1105	7	1.1691309055573633
1105	8	1.3694023916426197
1105	9	1.294441665629229
1105	10	1.7370813487069299
1105	11	1.4266305692295163
1105	12	1.5343212265572532
1105	13	1.609910413117344
1105	14	2.2899601626446895
1105	15	1.7088649397009448
1105	16	1.2122622366953024
1105	17	0.5428531916740909
1105	18	1.0461957538641116
1105	19	0.9954024761435676
1105	20	0.9828793371148379
1105	21	0.9961278003320153
1105	22	0.970198055028419
1105	23	0.9960161700988088
1105	24	0.8504431798378889
1105	25	0.8444047726963824
1106	1	0.9128752311646124
1106	2	1.2328152761287505
1106	3	1.7560659640875258
1106	4	1.3591701025371172
1106	5	0.6697598264009557
1106	6	0.6772009146088855
1106	7	1.0645817435203995
1106	8	1.0224397465682742
1106	9	1.2502427706016022
1106	10	-1.9991741008558925
1106	11	-0.12448704459297133
1106	12	0.7266910691217738
1106	13	1.4360300605638798
1106	14	0.7192109315744304
1106	15	1.1358028734949421
1106	16	0.7717276024288466
1106	17	-5.538074052952194
1106	18	-1.7230121001018972
1106	19	-0.0777057588246528
1106	20	0.5827027965621738
1106	21	0.9015697782897298
1106	22	0.9292386681495834
1106	23	0.5259752997516856
1106	24	0.7319994188865486
1106	25	0.9367642822522164
1107	1	0.5846083586911917
1107	2	0.9775839293698816
1107	3	1.3148181804819572
1107	4	0.941235180906201
1107	5	0.28728284301489637
1107	6	0.42108410324345513
1107	7	0.6425704052007717
1107	8	0.8411862347719179
1107	9	0.6600926351583141
1107	10	-2.2229955037379163
1107	11	-0.21076081275866088
1107	12	0.12064629740766719
1107	13	1.1694374376865362
1107	14	1.417442407326913
1107	15	1.1542839409498526
1107	16	-5.277070242721976
1107	17	-2.115125871215504
1107	18	-0.3592053019200492
1107	19	0.4550367546267573
1107	20	0.8177171279708091
1107	21	0.9793463657309971
1107	22	0.6469448994154483
1107	23	0.2801255051576099
1107	24	0.435026614674797
1107	25	0.645496712338776
1108	1	-0.5404411070784967
1108	2	-3.1953484014571885
1108	3	-0.2372198092291633
1108	4	-0.7718633549070191
1108	5	-0.9703348855169622
1108	6	-0.5765415398115508
1108	7	-0.16708531304220386
1108	8	0.05012964593139557
1108	9	-0.45150214125640886
1108	10	-3.553822571407089
1108	11	-1.4624426489280324
1108	12	-5.6561641497628905
1108	13	-1.7490429738686686
1108	14	-5.7064198799702766
1108	15	-2.6644734789155997
1108	16	-7.731363871530842
1108	17	-3.389364098362318
1108	18	-2.0980260379707047
1108	19	-0.7756715951951634
1108	20	-0.7366952186853055
1108	21	-0.23315858084596996
1108	22	-0.32654144803211693
1108	23	-0.43772174454559476
1108	24	-0.14312930143125513
1108	25	-0.4391886853660125
1109	1	-8.103913711586003
1109	2	-5.9014313418865605
1109	3	-5.011933325295505
1109	4	-4.983595560722353
1109	5	-2.435248635307616
1109	6	-1.5540736034500071
1109	7	-1.1355206235681656
1109	8	-0.428199978339066
1109	9	-5.171700242482199
1109	10	-1.3043202446022342
1109	11	-0.9686033118922843
1109	12	-5.40059757861793
1109	13	-7.8671023600090955
1109	14	-7.559177584065772
1109	15	-3.472389022695765
1109	16	-1.937265650102649
1109	17	-1.6389366321525216
1109	18	-1.0260040319468189
1109	19	-0.8820382708155421
1109	20	-0.5682507224876936
1109	21	-0.4818688367720796
1109	22	-0.5952458492523611
1109	23	-0.8522103322764139
1109	24	-0.5830088485126943
1109	25	-0.4495568962616119
1110	1	-2.4458721626608657
1110	2	-2.185123264535175
1110	3	-1.4562642258685727
1110	4	-2.2414676973725527
1110	5	-5.238212672469892
1110	6	-3.8757504687356246
1110	7	-8.245256859950242
1110	8	-9.434830548897018
1110	9	-11.030248672933944
1110	10	-9.01913568686992
1110	11	-11.604759957258182
1110	12	-6.516524817175263
1110	13	-4.738731676916217
1110	14	-4.297870670012006
1110	15	-9.71918204833123
1110	16	-11.356428663111252
1110	17	-12.091346465292727
1110	18	-8.950907862245415
1110	19	-11.961089200663046
1110	20	-12.736413864249627
1110	21	-13.738729017433332
1110	22	-14.534800001321335
1110	23	-15.127148383854546
1110	24	-15.500840281040304
1110	25	-15.901329739248057
1111	1	-0.35078646683227177
1111	2	0.18700860004724973
1111	3	-2.515821312681439
1111	4	-2.77796463224103
1111	5	-0.8160580409025151
1111	6	-0.41238781870344
1111	7	0.1734111704924679
1111	8	0.3781239776954912
1111	9	-3.754341526343719
1111	10	-3.677612070889513
1111	11	-0.9716740326382123
1111	12	-5.508054152940062
1111	13	-0.9966867754871664
1111	14	0.10129805465551556
1111	15	0.1881798749518815
1111	16	0.13845650380551433
1111	17	0.0775935352904682
1111	18	0.45495727196764335
1111	19	0.6365614259337242
1111	20	0.5626411376457519
1111	21	0.7494947315600982
1111	22	0.25338763452354485
1111	23	0.16244542173110332
1111	24	0.3964687286294968
1111	25	0.5509571651373371
1112	1	-0.4761267630126724
1112	2	0.13580871997568167
1112	3	-2.41309217162512
1112	4	-0.6920907751769647
1112	5	-0.668630371608792
1112	6	-0.7357089676576614
1112	7	-0.13293736005994816
1112	8	-3.7082760744263226
1112	9	-1.2103609549915042
1112	10	-3.3939178286760026
1112	11	-1.171928126485529
1112	12	-0.03187145451709483
1112	13	0.31113431698667426
1112	14	0.9146022685443818
1112	15	0.416888618823684
1112	16	0.3497729099158917
1112	17	-0.10149708080717801
1112	18	0.08808937226413249
1112	19	0.20143626948156168
1112	20	0.24300109186421892
1112	21	0.1447480570027686
1112	22	0.11134392549538674
1112	23	-0.22460852087849403
1112	24	0.011359852703698436
1112	25	-0.09764286795157062
1113	1	-1.347721628193554
1113	2	-0.7539339366999194
1113	3	-2.8903964988580277
1113	4	-1.3650493033517144
1113	5	-1.5124944244917877
1113	6	-1.0698167156828404
1113	7	-0.9531407130238883
1113	8	-7.96330151830168
1113	9	-9.230884388359247
1113	10	-3.5222136295082613
1113	11	-8.760669778938777
1113	12	-7.623084467654504
1113	13	-3.0169357194772424
1113	14	-1.3846630070639065
1113	15	-6.909080554768156
1113	16	-2.8603546353999505
1113	17	-2.1300109719996456
1113	18	-1.5402811057440573
1113	19	-1.4352114102036886
1113	20	-6.826776271132044
1113	21	-2.6665274539170163
1113	22	-2.0924954715421187
1113	23	-2.239840601863211
1113	24	-7.41887103592169
1113	25	-4.707878344478598
1114	1	-4.154495487036151
1114	2	-4.293749796395815
1114	3	-1.087225165570139
1114	4	-0.7611705925862964
1114	5	-0.9700517758402718
1114	6	-0.904945000431308
1114	7	-0.6132195798061808
1114	8	-0.3512256102859972
1114	9	-0.7476284323267208
1114	10	-3.2849202950641256
1114	11	-1.4292349033609746
1114	12	-4.797810743875058
1114	13	-1.483876804173196
1114	14	-5.784342642317661
1114	15	-2.148398574212198
1114	16	-1.3008296537660158
1114	17	-1.2342582663186619
1114	18	-0.856711348781996
1114	19	-0.5438943298903425
1114	20	-0.32909759015625895
1114	21	-0.19508636795878687
1114	22	-0.18971948198363364
1114	23	-0.6716854635307747
1114	24	-0.6263015288895701
1114	25	-0.4177096796346511
1115	1	0.6925200584611098
1115	2	0.8455779268940624
1115	3	1.5190726669763102
1115	4	0.48548909668522455
1115	5	0.4450463757246581
1115	6	-0.2590270865968449
1115	7	-2.014081214007696
1115	8	-0.06812528777427929
1115	9	0.03608880140074433
1115	10	0.7751166061921211
1115	11	-0.09079207225642705
1115	12	0.6592869712624285
1115	13	0.8593756509580253
1115	14	1.3115425545805266
1115	15	0.5956031569802498
1115	16	0.2464731989058464
1115	17	-0.16742679763142476
1115	18	0.08199358074616114
1115	19	0.47148981321551986
1115	20	0.5797232497231093
1115	21	0.5999888673846385
1115	22	0.1256742032469269
1115	23	0.18449339490144823
1115	24	0.15752806297818012
1115	25	0.35497347056096373
1116	1	0.3969570527492472
1116	2	0.5538833392481912
1116	3	1.2714527074523225
1116	4	0.5057751971549997
1116	5	-0.1906050074012242
1116	6	-1.177411559433608
1116	7	-4.771547589328414
1116	8	-1.1441969914882364
1116	9	-1.1961699518079065
1116	10	-0.4973307613568636
1116	11	-2.258727781030913
1116	12	-0.5502766813852418
1116	13	-6.849068767549031
1116	14	-1.7572524409633594
1116	15	-1.3943230025993998
1116	16	-0.9923974376866127
1116	17	-0.5163722717347845
1116	18	-0.5003869201510724
1116	19	-0.2624176585411604
1116	20	-0.08524068932415929
1116	21	0.026044485681953944
1116	22	-0.3416391157419909
1116	23	-0.2605710215469088
1116	24	-0.0853598221077192
1116	25	-0.13964649277543018
1201	1	2.06044670702218
1201	2	2.2567355574082946
1201	3	2.7846398045694762
1201	4	2.420649067027764
1201	5	1.8994451133342096
1201	6	1.9254209405115361
1201	7	2.0705634402931032
1201	8	2.1208199899744784
1201	9	2.5729183156378426
1201	10	2.518314313246293
1201	11	2.251200865610329
1201	12	2.447276312862787
1201	13	2.7110062667492265
1201	14	3.3691767046599885
1201	15	2.7406611594707293
1201	16	2.249085792404248
1201	17	2.059900132623376
1201	18	2.1204579219131325
1201	19	2.10560721607974
1201	20	2.3029863928096255
1201	21	2.0742647931563027
1201	22	1.3847646249084704
1201	23	0.859059666794959
1201	24	1.3774033488408577
1201	25	1.553539627702861
1202	1	2.059169177794246
1202	2	2.38201183582823
1202	3	3.0978833863847868
1202	4	2.4979173176270812
1202	5	2.0314734327470525
1202	6	1.8119633979993317
1202	7	2.152198385043782
1202	8	2.433596745559214
1202	9	2.567497723495414
1202	10	-1.3878257797944826
1202	11	1.095727530851864
1202	12	2.217488377624864
1202	13	2.832442959068274
1202	14	3.3610839896304405
1202	15	2.934413290806841
1202	16	2.6356023239378423
1202	17	2.068376010988338
1202	18	2.3964521106820733
1202	19	2.6443467948004766
1202	20	2.5836797005414773
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2211	13	-0.3592959591002831
2211	14	-4.881916339765823
2211	15	-1.2761173901022929
2211	16	-0.17242023970622
2211	17	0.21815278619892098
2211	18	0.8085330127572696
2211	19	1.0973610647171874
2211	20	1.2644091368032981
2211	21	1.1806741814550072
2211	22	1.086200492909061
2211	23	0.9571988380692282
2211	24	1.0131063277512666
2211	25	1.1720362562680542
2212	1	1.0957090145281825
2212	2	1.2909939652523441
2212	3	1.849366699905783
2212	4	1.5656650724865244
2212	5	1.1414458502549714
2212	6	1.0301921631952418
2212	7	1.131039618547689
2212	8	1.4575253700565547
2212	9	1.3798624810573799
2212	10	1.9044818857236905
2212	11	1.290964791362466
2212	12	1.4810787915479864
2212	13	1.6956713527828065
2212	14	-4.266835621800041
2212	15	-0.6449705376802086
2212	16	0.011269249093633249
2212	17	0.4489456196327133
2212	18	0.6342231179538231
2212	19	1.0447581523791598
2212	20	1.3559868668815476
2212	21	1.3760506607388976
2212	22	1.27487433996086
2212	23	1.2049600361698545
2212	24	1.28888302850158
2212	25	1.3592233872141684
2213	1	0.5332611283633035
2213	2	0.801598518395938
2213	3	1.511967308181731
2213	4	0.937682746016069
2213	5	0.6322509559412843
2213	6	0.17373353096706623
2213	7	0.5303230329046542
2213	8	-4.764256144798747
2213	9	-1.3178410812974342
2213	10	0.3872535688889869
2213	11	0.23420506907069694
2213	12	0.7768167778802848
2213	13	-7.416180176941943
2213	14	-1.8537443542827177
2213	15	-0.5169985523363074
2213	16	-0.05825496111643247
2213	17	0.09996171695686584
2213	18	0.37300081856623635
2213	19	0.5949874532536938
2213	20	0.7998097854304795
2213	21	0.9363984818091069
2213	22	0.5373209743051568
2213	23	0.5206506819133381
2213	24	0.6459430788791956
2213	25	0.7811035237605815
2214	1	-1.102792175444936
2214	2	-0.7912463542444925
2214	3	-7.007729067207116
2214	4	-2.5707904168235984
2214	5	-1.6815866509613286
2214	6	-1.3712473295127303
2214	7	-0.8249269434629554
2214	8	-5.992131473343306
2214	9	-1.909774477070986
2214	10	-0.2899861026696726
2214	11	-0.7410732770313722
2214	12	-0.1548488058615014
2214	13	-6.097899273108933
2214	14	-1.3682449991481853
2214	15	-0.807610136879962
2214	16	-0.7090751137327835
2214	17	-0.6449235002492557
2214	18	-0.6013892756047667
2214	19	-0.44373313256882696
2214	20	-0.034318673726772886
2214	21	0.011123422510820546
2214	22	-0.2087431460755269
2214	23	-0.37041540511688
2214	24	0.005248590821974375
2214	25	0.0691349673356676
2215	1	0.9105387355502899
2215	2	1.3142695378296771
2215	3	-1.964410904605895
2215	4	0.20979983841249705
2215	5	0.6083781861139563
2215	6	0.14224962327583057
2215	7	0.7708529424668527
2215	8	1.127559690402947
2215	9	1.4801964207890812
2215	10	2.222043962379292
2215	11	1.2748960758472272
2215	12	1.1417957866070338
2215	13	1.7795686999408744
2215	14	1.9122009975552778
2215	15	1.7579456970995828
2215	16	1.4476444813178837
2215	17	1.3049492158610683
2215	18	1.5016167995540002
2215	19	1.537495772306638
2215	20	1.850582577714146
2215	21	1.9885276609998854
2215	22	1.712643288821198
2215	23	1.6104099907864722
2215	24	1.555936089793935
2215	25	1.6221959526118148
2216	1	0.4579557404519967
2216	2	0.273513547879741
2216	3	1.360769312321299
2216	4	0.06512903101203804
2216	5	-0.18220202514933703
2216	6	-0.5560738028083563
2216	7	-1.1563465140428058
2216	8	-6.059338958453509
2216	9	-1.9089335896647626
2216	10	-0.6178460910592563
2216	11	-1.0952108591053715
2216	12	-0.0479022272841263
2216	13	0.47877246588314293
2216	14	1.224024622637078
2216	15	0.6899786487899071
2216	16	0.2183957300393189
2216	17	-0.10987706546583098
2216	18	0.14702269530361178
2216	19	0.3528649654055762
2216	20	0.5108297062562492
2216	21	0.4096780164093161
2216	22	0.3224015105285929
2216	23	-0.18038063723293973
2216	24	-0.1950760269834042
2216	25	-0.06005875885078282
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	162443.0
3	1.0
4	935.0
5	7428.0
6	12355.0
7	10591.0
8	9123.0
9	7554.0
10	6835.0
11	6186.0
12	6062.0
13	5884.0
14	5930.0
15	5721.0
16	5690.0
17	5873.0
18	6716.0
19	8754.0
20	12703.0
21	16270.0
22	19753.0
23	22659.0
24	25381.0
25	28052.0
26	30454.0
27	32837.0
28	35074.0
29	40568.0
30	46229.0
31	51977.0
32	64136.0
33	81024.0
34	100600.0
35	126594.0
36	160233.0
37	188514.0
38	247715.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.54454782536385	18.997530904336767	12.54155539896622	21.91636587133317
2	13.758623212283744	23.315640363980375	41.27774543758367	21.647990986152212
3	15.222026977168834	31.961566601839753	29.931400198858128	22.885006222133285
4	9.656031963518865	18.67538254976477	38.57760089752	33.09098458919637
5	11.136988460258813	42.242382094764466	33.18282222094364	13.43780722403308
6	27.335309673503854	40.06439043781372	19.535078787969702	13.065221100712721
7	26.366776556364414	35.50218591736645	22.3154846960781	15.815552830191038
8	25.202257508202504	36.765892598966005	21.034343303015284	16.99750658981621
9	27.387535714977755	18.32000995112572	19.852808348944475	34.43964598495205
10	12.392045351434192	33.868082094656536	33.24580775202142	20.49406480188785
11	31.77555489020334	27.324056878863324	23.150652479246116	17.749735751687222
12	21.293838278966064	30.748536249811696	30.071338874342196	17.88628659688004
13	26.269067180854222	25.816736304912386	27.05753671242894	20.856659801804447
14	19.95537067653429	24.912600908212983	29.844828717782036	25.28719969747069
15	22.149783182892637	32.60200529261247	24.34389230771154	20.904319216783357
16	20.974646587949394	32.104883858583925	26.00045127735869	20.92001827610799
17	20.11926257453911	31.83420739637962	26.806641754151094	21.23988827493017
18	19.616251467229684	30.529940235781776	29.642414719423183	20.211393577565357
19	21.195448363450904	30.082714981449442	29.414882446473396	19.30695420862626
20	21.619842763869006	29.380323815750415	29.30824368719604	19.69158973318454
21	22.529358931809277	28.904101968887563	28.76697174079762	19.79956735850554
22	21.65109287397416	29.31014869586414	28.591358263902343	20.447400166259353
23	21.0850756781723	29.607706004816254	28.627830392393992	20.679387924617455
24	20.67653171242402	29.781708517586765	29.15749175742613	20.38426801256309
25	20.814984994256445	29.355759243125547	29.309073318775493	20.52018244384252
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15140.0
1	15140.0
2	14692.0
3	14244.0
4	14244.0
5	14244.0
6	18592.0
7	22940.0
8	22940.0
9	22940.0
10	20898.5
11	18857.0
12	18857.0
13	18857.0
14	19653.5
15	20450.0
16	20450.0
17	20450.0
18	27753.0
19	35056.0
20	35056.0
21	35056.0
22	46462.5
23	57869.0
24	57869.0
25	57869.0
26	76756.0
27	95643.0
28	95643.0
29	95643.0
30	112311.5
31	128980.0
32	128980.0
33	128980.0
34	140457.5
35	151935.0
36	151935.0
37	151935.0
38	160509.0
39	169083.0
40	169083.0
41	169083.0
42	176767.0
43	184451.0
44	184451.0
45	184451.0
46	187219.0
47	189987.0
48	189987.0
49	189987.0
50	178295.0
51	166603.0
52	166603.0
53	166603.0
54	147804.0
55	129005.0
56	129005.0
57	129005.0
58	109481.0
59	89957.0
60	89957.0
61	89957.0
62	72882.5
63	55808.0
64	55808.0
65	55808.0
66	43002.5
67	30197.0
68	30197.0
69	30197.0
70	22614.5
71	15032.0
72	15032.0
73	15032.0
74	10833.5
75	6635.0
76	6635.0
77	6635.0
78	5288.0
79	3941.0
80	3941.0
81	3941.0
82	2830.5
83	1720.0
84	1720.0
85	1720.0
86	1286.0
87	852.0
88	852.0
89	852.0
90	628.0
91	404.0
92	404.0
93	404.0
94	228.0
95	52.0
96	52.0
97	52.0
98	32.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3416510162295137
2	0.3450158082915954
3	0.3566679585806559
4	0.36021968353507544
5	0.36501762777174745
6	0.30700612018289514
7	0.31579196612277505
8	0.3138603262352837
9	0.3137357043070585
10	0.4265185493509067
11	0.3136110823788332
12	0.3131125946659322
13	0.3065076324699942
14	0.31211561924013026
15	0.30457599258250284
16	0.3085015833215981
17	0.30251973076678623
18	0.3053237241518543
19	0.3054483460800796
20	0.49973393218323914
21	0.30625838861354365
22	0.30806540657280973
23	0.30251973076678623
24	0.30713074211112035
25	0.299092627740592
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1604854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.42974180404073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.01772489861989	52.22616889289184
2	8.29758264906774	10.194367194598392
3	2.7310720266277206	5.033071483319373
4	1.2739243386587629	3.130273728067646
5	0.6674440982626157	2.050045931245164
6	0.4107063242013536	1.5137750071785476
7	0.27527848128548865	1.1837200220703215
8	0.19425235657934478	0.954629768759648
9	0.15648240343163294	0.8651406275712854
>10	0.8441928072809027	9.941421665562416
>50	0.0844044739041536	3.632712962246945
>100	0.04329284362997759	4.8587685212595435
>500	0.002081313400239152	0.8249175229849587
>1k	0.0011447223701315336	1.7653516271876655
>5k	4.162626800478305E-4	1.8256350450561416
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	9416	0.5867200380844613	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8270	0.5153116732113949	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5646	0.35180770337987133	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5226	0.3256370984525695	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	4621	0.2879389651644324	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4538	0.28276715514308465	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3743	0.23322993867354913	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3431	0.21378891787041063	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2650	0.16512405489845183	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2181	0.1359002127296315	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	2013	0.12543197075871076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	220	0.0	15.535479	2
TATCACG	140	0.0	14.240854	2
TAACGCA	255	0.0	14.164605	4
ATAACGC	255	0.0	14.147778	3
CGCATAT	60	4.0883909E-4	12.668043	8
TGACCGA	85	3.9167335E-6	12.301612	19
GGTATCA	2095	0.0	12.2355795	1
ATCACGC	165	0.0	12.08315	3
GTTATCA	350	0.0	11.935194	1
CGAAAGG	80	2.8477454E-5	11.88261	18
CTCGAAC	65	7.9806324E-4	11.6998005	18
CACGCCC	90	7.405213E-6	11.6189165	16
GAACAAA	1215	0.0	11.564591	1
CCAATCG	75	2.0609806E-4	11.407663	15
TCACGCA	185	0.0	11.303476	4
GTATAAC	370	0.0	11.290049	1
CGAAACC	110	4.926951E-7	11.234468	18
GTATCAA	5170	0.0	11.201705	1
CGGTGAG	85	5.286534E-5	11.183984	15
CGCGCCA	60	0.0058737686	11.083844	10
>>END_MODULE