FastQCFastQC Report
Thu 26 May 2016
SRR1364316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364316_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604854
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT225001.4019966925340248No Hit
GTATCAACGCAGAGTACTTTTTTTT223921.3952671084098616No Hit
TATCAACGCAGAGTACTTTTTTTTT177521.1061442349272892No Hit
GGTATCAACGCAGAGTACTTTTTTT145660.9076215032644713No Hit
GAGTACTTTTTTTTTTTTTTTTTTT108060.6733322782010077No Hit
ACGCAGAGTACTTTTTTTTTTTTTT79090.4928174151667379No Hit
GCAGAGTACTTTTTTTTTTTTTTTT31530.19646646984710137No Hit
ATCAACGCAGAGTACTTTTTTTTTT28800.17945557664435519No Hit
GTATAACGCAGAGTACTTTTTTTTT28040.1747199433717958No Hit
GTGGTATCAACGCAGAGTACTTTTT27350.1704204868480248No Hit
GTGGTATCAACGCAGAGTACATGGG19400.12088327037848925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA3950.016.3578914
CTCGACG350.002173953716.28154813
CGACATG350.002174406316.2810415
TATAACG3800.015.9993972
ATAACGC4100.015.5257423
TATCACG1400.014.9280082
CAACGAG456.7564414E-414.7795241
TCGTCAT400.00528817114.24635414
GTATAAC5000.013.4915941
TGTACGG500.001495436513.3048895
CTACGGA500.001498250213.3015711
GGTATCA35450.013.1595231
GTATCAC1950.012.6681631
ATCACGC1800.012.66776853
TCACGCA1850.012.3269334
TCGTTGT853.9109636E-612.30324119
CGGTTTG1405.2750693E-1112.21116114
GCGCCAC701.093552E-412.21116113
TTACGGA550.00305151712.09988219
AAGCGTG550.003065089712.0927147
Produced by FastQC (version 0.11.5)