##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1364316_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1604854
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.684228596495384	31.0	30.0	34.0	26.0	34.0
2	29.66470781765818	31.0	30.0	34.0	26.0	34.0
3	29.71342938360748	31.0	30.0	34.0	26.0	34.0
4	33.06471554421773	35.0	33.0	37.0	27.0	37.0
5	32.709106248917344	35.0	33.0	37.0	26.0	37.0
6	32.60049574603048	35.0	33.0	37.0	25.0	37.0
7	32.32620786688384	35.0	33.0	37.0	23.0	37.0
8	32.20611594574958	35.0	32.0	37.0	22.0	37.0
9	32.93983938725891	37.0	32.0	39.0	17.0	39.0
10	33.07442421553612	37.0	32.0	39.0	17.0	39.0
11	33.01277935562986	37.0	32.0	39.0	17.0	39.0
12	32.95021042412581	37.0	32.0	39.0	17.0	39.0
13	33.0004773019851	37.0	32.0	39.0	17.0	39.0
14	33.9063977159293	38.0	33.0	41.0	17.0	41.0
15	33.946573956260195	38.0	33.0	41.0	17.0	41.0
16	33.906945429303846	38.0	33.0	41.0	17.0	41.0
17	33.916197984364935	38.0	33.0	41.0	17.0	41.0
18	33.88227153373453	38.0	33.0	41.0	17.0	41.0
19	33.81799216626559	39.0	33.0	41.0	17.0	41.0
20	33.71631562746518	39.0	33.0	41.0	11.0	41.0
21	33.64070501117236	39.0	33.0	41.0	10.0	41.0
22	33.58041541473555	39.0	33.0	41.0	10.0	41.0
23	33.30995218256614	38.0	32.0	41.0	2.0	41.0
24	33.225597468679396	38.0	32.0	41.0	2.0	41.0
25	33.07393133580999	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.30841167890326204
1101	2	0.5141394001368234
1101	3	-1.5556788751431831
1101	4	-0.3881173883723861
1101	5	0.5116101256512096
1101	6	0.8372925876881823
1101	7	1.1945655712147527
1101	8	1.0555575099503862
1101	9	1.924642274603876
1101	10	1.5698897047803015
1101	11	1.5517546117616945
1101	12	1.5473749570942132
1101	13	1.4143654435660977
1101	14	1.4598075067253617
1101	15	1.297029077309098
1101	16	1.3498212838779793
1101	17	1.4421495106569608
1101	18	1.4434831006323208
1101	19	1.4876243025808478
1101	20	1.7549827686078956
1101	21	1.6128985200670982
1101	22	1.646865738660594
1101	23	2.0744522964573235
1101	24	1.9891444620369967
1101	25	1.9704179531424586
1102	1	0.4565750330577316
1102	2	0.48767053749386235
1102	3	0.57205683745428
1102	4	0.5704309668350547
1102	5	0.7393310902114791
1102	6	0.6873784676340051
1102	7	0.7623347341638222
1102	8	0.8804081458226314
1102	9	1.3840370319020607
1102	10	1.136743072581531
1102	11	1.1192547357353746
1102	12	1.1049930911261754
1102	13	0.9006645814177361
1102	14	1.073874719133805
1102	15	1.212167486672108
1102	16	1.1482252757659168
1102	17	1.019824653258894
1102	18	1.1898492229096505
1102	19	1.1832327041963069
1102	20	1.4106489451760353
1102	21	1.436814201223548
1102	22	1.4690839464971504
1102	23	1.750900969101778
1102	24	1.8623804132435353
1102	25	1.494668975039751
1103	1	-2.187863753776572
1103	2	-2.455137552018236
1103	3	-2.588773786754196
1103	4	-2.205632119869996
1103	5	-6.110596448858669
1103	6	-9.009499837171916
1103	7	-9.957900796498247
1103	8	-10.757065252529696
1103	9	-12.115457047191324
1103	10	-10.983829303484164
1103	11	-12.182930189780016
1103	12	-11.758558408358109
1103	13	-11.824525710123265
1103	14	-12.498870179225054
1103	15	-11.309019175294392
1103	16	-6.260637152915557
1103	17	-4.496182031425089
1103	18	-3.798521124250012
1103	19	-3.4050488620042607
1103	20	-8.456780193256982
1103	21	-5.293190498944874
1103	22	-11.042679530583001
1103	23	-12.059669187439948
1103	24	-13.117033501866203
1103	25	-13.50862440686199
1104	1	0.24703933846917536
1104	2	0.4418223645220998
1104	3	0.42469712461881315
1104	4	0.5457135042029009
1104	5	0.57211664224603
1104	6	0.3744569985331694
1104	7	0.4729042958453036
1104	8	0.583916376563824
1104	9	1.0066255322339117
1104	10	0.8362336172780545
1104	11	0.8574727639096551
1104	12	0.6150148715088761
1104	13	0.5322610928173859
1104	14	0.8067682383014727
1104	15	0.7369097209841513
1104	16	0.5274600645522867
1104	17	0.3147394480278578
1104	18	0.47552324629785403
1104	19	0.6843736036419799
1104	20	1.0187834039849335
1104	21	0.9171637156051418
1104	22	0.5495059063576804
1104	23	1.106369901676068
1104	24	1.217056184671037
1104	25	1.3576054006265608
1105	1	0.42796205121104336
1105	2	0.53081647875727
1105	3	0.1393178228263956
1105	4	0.34023022239649947
1105	5	0.5445949032179698
1105	6	0.6959820701332404
1105	7	0.8085500228773626
1105	8	0.6028548982235797
1105	9	0.9018808946844743
1105	10	0.5604117175864332
1105	11	0.9941358437053651
1105	12	1.2157240830173137
1105	13	1.0415525013588436
1105	14	0.9562442176467485
1105	15	0.8240245346042201
1105	16	0.8930397601941209
1105	17	0.8881664638375639
1105	18	0.9685348129755837
1105	19	0.9192120884911787
1105	20	1.296071917913693
1105	21	1.1879702749482348
1105	22	0.8592519135110166
1105	23	1.4078632800925206
1105	24	1.3482593718845095
1105	25	1.6331047173943603
1106	1	0.09820276634318503
1106	2	0.21807697830327655
1106	3	0.11650385875448777
1106	4	-1.3885689720685264
1106	5	-0.11054752741346618
1106	6	0.1541619656159554
1106	7	0.5568082513599677
1106	8	0.5675676735597825
1106	9	0.9602700885559017
1106	10	0.6107521572449599
1106	11	0.7412970144572313
1106	12	0.6746866489452117
1106	13	0.48264226002812904
1106	14	-0.07309053445997193
1106	15	0.5022810262619615
1106	16	0.41469730669815874
1106	17	0.4094876379311714
1106	18	0.45114457920775664
1106	19	-0.7831203134056963
1106	20	0.35319586784916623
1106	21	0.07866792891556429
1106	22	0.6512385553491455
1106	23	0.5104678578352235
1106	24	0.6416761460950795
1106	25	0.842615262559498
1107	1	0.0012083174410513209
1107	2	-0.12753516196306336
1107	3	-0.24949911028485872
1107	4	-0.05847104624204036
1107	5	-0.007609533985579731
1107	6	-0.041380321331423886
1107	7	-0.05107631903665322
1107	8	-0.30148960178910045
1107	9	0.5457163299226195
1107	10	0.4068865029959312
1107	11	0.21951677146825688
1107	12	0.03395680747616581
1107	13	0.17853217382104702
1107	14	0.0934730101969592
1107	15	-0.04243056370452081
1107	16	-0.22003761143462697
1107	17	-0.3561211353833329
1107	18	-0.2483407581542636
1107	19	-0.018450569840616993
1107	20	0.16607642632590114
1107	21	-0.006455828427782251
1107	22	-0.03327040533403647
1107	23	0.1787378349005948
1107	24	0.5177668526521089
1107	25	0.18630343034738672
1108	1	-0.23701882937335128
1108	2	-0.1528616360429993
1108	3	-0.06548644704781736
1108	4	-0.2856178606464681
1108	5	-0.03135310343552078
1108	6	-0.1153811127837372
1108	7	-0.4741075814030964
1108	8	-0.024877017697289006
1108	9	0.250900342585858
1108	10	0.15283030988156554
1108	11	0.026798132766238325
1108	12	0.1938301751057665
1108	13	-0.40001946844250824
1108	14	-0.19698998940731371
1108	15	-0.524820749695813
1108	16	-0.9509438243352974
1108	17	-1.0708025758265407
1108	18	-0.9467974261400158
1108	19	-0.834042180446076
1108	20	-0.2759496916004949
1108	21	-0.5201083808614939
1108	22	-0.3680547345782017
1108	23	-0.7088759363696866
1108	24	-0.5597954742177009
1108	25	-0.5327309978283168
1109	1	0.121460975989379
1109	2	0.12892647092461118
1109	3	0.09374036682464748
1109	4	0.13474167025334083
1109	5	0.1684058732002427
1109	6	0.3050793986478624
1109	7	0.4374596070684831
1109	8	0.2620182608520132
1109	9	1.2040279014101074
1109	10	0.539195896829753
1109	11	0.3960989376877748
1109	12	0.7256054082153867
1109	13	0.6059691564794534
1109	14	0.3200512098764463
1109	15	-0.023864527462563956
1109	16	0.2780579081071153
1109	17	0.23962967188101914
1109	18	0.04473505471968764
1109	19	0.4159523684608928
1109	20	0.7914960251780343
1109	21	0.820235135422891
1109	22	0.462373127512393
1109	23	0.7907695492824942
1109	24	0.4961935141104661
1109	25	0.48915688037895677
1110	1	0.10835433886821377
1110	2	0.18064645728127715
1110	3	-0.025513225280288765
1110	4	0.1396663721442053
1110	5	0.12443277135184161
1110	6	0.21478717376774625
1110	7	0.31538699470861786
1110	8	0.2658650489082284
1110	9	-3.450067470924189
1110	10	-1.2581011867944092
1110	11	-0.2992282200034282
1110	12	0.18592159928532226
1110	13	-0.17985939378064586
1110	14	0.13036277034420607
1110	15	0.21550191580578826
1110	16	-0.3328676024450061
1110	17	-0.0731275241877114
1110	18	0.015998215229913626
1110	19	-0.03632961488249009
1110	20	0.24881384486464242
1110	21	-0.02391551048964402
1110	22	0.25955139666105964
1110	23	0.5680064124899857
1110	24	0.5992454197726786
1110	25	0.6929042799005387
1111	1	-0.018238621086567264
1111	2	0.05562677449378839
1111	3	0.041458047943720544
1111	4	-0.2979747439090943
1111	5	-0.10286114257325352
1111	6	-0.10689350076306425
1111	7	-0.033005745875605896
1111	8	0.11630278064361121
1111	9	0.7344164362941825
1111	10	0.2089696779696979
1111	11	0.40824989340980267
1111	12	0.39470135618326907
1111	13	0.23301124264069273
1111	14	0.18651580037195004
1111	15	0.14516068019776895
1111	16	0.06883173428430212
1111	17	0.12193372236639988
1111	18	0.12805439921407213
1111	19	0.24294659687065945
1111	20	0.35178701122513445
1111	21	0.38415230147973034
1111	22	0.3499706456421521
1111	23	0.6269995958528156
1111	24	0.570392135607058
1111	25	0.7721845291485536
1112	1	-0.23730920286163837
1112	2	-0.2150409352124356
1112	3	-0.1988761530308203
1112	4	-0.17215349730187768
1112	5	-0.1822175105514532
1112	6	-0.1488040548423868
1112	7	-0.03594905993322328
1112	8	-0.46845466515219414
1112	9	0.35355844425995286
1112	10	-0.19677813961781965
1112	11	0.026456544038758523
1112	12	-0.6169768863222203
1112	13	-0.3200915512001856
1112	14	-1.1950347361464182
1112	15	-0.6063214208000076
1112	16	-0.7375413037765313
1112	17	-0.7507454731418335
1112	18	-0.750100301529649
1112	19	-0.5690565322480197
1112	20	-0.46323951494790805
1112	21	-0.7511470920461534
1112	22	-0.4901521724166642
1112	23	-0.18605154973369764
1112	24	-0.341243592026899
1112	25	-0.4871554331264747
1113	1	-1.8749722445909143
1113	2	-1.7455430546414554
1113	3	-2.0795968991754563
1113	4	-1.95489077474533
1113	5	-1.5313997335878362
1113	6	-1.4658701412550172
1113	7	-1.8955576195053894
1113	8	-2.053226049848572
1113	9	-10.852459380893137
1113	10	-3.9731521790259166
1113	11	-2.413475363579387
1113	12	-12.658024990800953
1113	13	-4.280325748725851
1113	14	-3.418787359130654
1113	15	-2.630113734084375
1113	16	-2.4249174778791485
1113	17	-2.645538947429369
1113	18	-2.978233710447835
1113	19	-3.553344206883274
1113	20	-14.979003000366877
1113	21	-17.678461857580448
1113	22	-11.893892880059237
1113	23	-18.050887099040025
1113	24	-19.200923940707423
1113	25	-15.861206393491457
1114	1	-0.4766318804000669
1114	2	-0.2956527461168257
1114	3	-0.3452358236183528
1114	4	-0.8108705379935088
1114	5	-0.4240990022271802
1114	6	-0.36622130544888876
1114	7	-0.6514114079636748
1114	8	-0.3114983523092434
1114	9	0.244605583220455
1114	10	-0.34903797871337616
1114	11	-0.5700627847820314
1114	12	-0.522718220728926
1114	13	-0.5505012803318934
1114	14	-0.6961035659514678
1114	15	-0.5054958870667505
1114	16	-0.43722084738921296
1114	17	-0.6499442782583529
1114	18	-0.6771943553175817
1114	19	-0.7728420244142171
1114	20	-1.0764150894025235
1114	21	-1.2100577991220902
1114	22	-1.2766984572837998
1114	23	-0.909162935863538
1114	24	-0.38110323388534795
1114	25	-0.7818397513128765
1115	1	-0.31569963750021657
1115	2	-0.28443114698072947
1115	3	-0.27972513016296574
1115	4	-0.261392806756902
1115	5	-0.5236840029749743
1115	6	-0.2376854076279571
1115	7	-0.548341641422116
1115	8	-0.2666083264305783
1115	9	0.3106940969369987
1115	10	-0.13827164931772273
1115	11	-0.09465084400006418
1115	12	-0.03355911782172427
1115	13	-0.18611566537975932
1115	14	-0.4426239039090305
1115	15	-0.4701777766735873
1115	16	-0.20748413895646678
1115	17	-0.19266403785125163
1115	18	-0.24454670908451703
1115	19	-0.04544540486689641
1115	20	-0.7692752664263267
1115	21	0.019168987844913943
1115	22	-0.5905115674372752
1115	23	-0.1006667248129105
1115	24	0.3407187282144264
1115	25	0.3975561248258117
1116	1	-0.6787194845875852
1116	2	-0.550089940835484
1116	3	-0.48789461561438685
1116	4	-0.3537445064003819
1116	5	-1.1829343199178055
1116	6	-0.9263692510366468
1116	7	-2.1696959191311898
1116	8	-1.417755376464875
1116	9	-0.292707858709484
1116	10	-1.3479357441750501
1116	11	-1.1013718208203471
1116	12	-2.0521469384779785
1116	13	-1.661364505455527
1116	14	-1.2214167263820386
1116	15	-2.4818487215429847
1116	16	-2.689436360136959
1116	17	-3.3990031289914846
1116	18	-2.7220319646080355
1116	19	-2.240614002614471
1116	20	-2.4984645936864034
1116	21	-1.7734059721028395
1116	22	-1.6468682982823566
1116	23	-1.8382660709561236
1116	24	-2.338334980067966
1116	25	-1.7722963438970751
1201	1	0.7085230775811979
1201	2	0.8050717346193856
1201	3	0.4986161782539291
1201	4	0.7947500326121641
1201	5	0.7869102554250915
1201	6	0.8927821116063583
1201	7	1.0432323686879954
1201	8	1.052945148663948
1201	9	1.7113680632175132
1201	10	1.3904109607659692
1201	11	1.3196003242649184
1201	12	1.4364855724079888
1201	13	1.287694197030298
1201	14	1.45936886213714
1201	15	1.3953908643497712
1201	16	1.3209623712215262
1201	17	1.3977357625914024
1201	18	1.4915159943867309
1201	19	1.552399289873108
1201	20	1.6678613310994237
1201	21	1.6755394588189674
1201	22	1.7738507242628572
1201	23	2.0348952750376625
1201	24	2.073660586733361
1201	25	2.0740185597046548
1202	1	0.8538181690186981
1202	2	0.9316183847952395
1202	3	0.8686321919046591
1202	4	0.9569862240990403
1202	5	0.9518250910513615
1202	6	0.9921020563315892
1202	7	1.1083031735823
1202	8	1.3248209280331231
1202	9	1.8894369009204013
1202	10	1.476427391776845
1202	11	1.64239149658885
1202	12	1.5447846453085887
1202	13	1.6105289504060778
1202	14	1.6101958841707003
1202	15	1.6052468305639636
1202	16	1.5273865681849728
1202	17	1.4214021824048118
1202	18	1.492758690584175
1202	19	1.5263802885344973
1202	20	1.7679164277284727
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2211	3	0.17416516682348515
2211	4	0.12159759532862324
2211	5	-0.5267659164306409
2211	6	0.030323198866057055
2211	7	0.30860815923004026
2211	8	0.32491268842086996
2211	9	0.8586804071083449
2211	10	0.08580724448965782
2211	11	0.5036991997557649
2211	12	0.5870282456097513
2211	13	0.39129044398285373
2211	14	0.43729990280511544
2211	15	0.4153901767594377
2211	16	0.4589947866217514
2211	17	0.5224544858258255
2211	18	0.2927013403418357
2211	19	0.13812297123178752
2211	20	0.54957023225532
2211	21	0.6903942502025657
2211	22	0.5467057039864187
2211	23	0.8340449051589047
2211	24	0.9040516313176141
2211	25	1.0437305041951888
2212	1	0.080864683130347
2212	2	0.1327337576232317
2212	3	0.19641081454459197
2212	4	0.09234329896182203
2212	5	-0.04110341271452711
2212	6	0.19818192947069946
2212	7	0.11560017061974293
2212	8	0.010429275219618006
2212	9	0.552390978857197
2212	10	0.08372949668318341
2212	11	0.33449612431807907
2212	12	0.26513143478081247
2212	13	0.06865777550336105
2212	14	-0.014813983303319844
2212	15	0.10113586675553421
2212	16	0.11935229670638847
2212	17	-0.10769467672016475
2212	18	0.03756601944734683
2212	19	0.21051112659647941
2212	20	0.5009455332941926
2212	21	0.7476588671229294
2212	22	0.18597262830326855
2212	23	0.4394637412601128
2212	24	0.6046153888282433
2212	25	0.5315367592579605
2213	1	-0.42749638982140326
2213	2	-0.41340753264456964
2213	3	-0.4915345342343933
2213	4	-0.401631089877597
2213	5	-0.4463433159651231
2213	6	-0.1416239512558377
2213	7	-0.2728792157051565
2213	8	-0.24440492930827062
2213	9	0.32666105860360517
2213	10	-0.24478523452371803
2213	11	-0.06846002277410435
2213	12	-0.003135621002492428
2213	13	-0.43613887440704957
2213	14	-0.32027072364960674
2213	15	-0.32191553844027965
2213	16	-0.43312102862980595
2213	17	-0.4489096720067849
2213	18	-0.6257889467970372
2213	19	-0.6018417844850674
2213	20	-0.17765225348701108
2213	21	-0.4791715667036627
2213	22	-0.24176847778362998
2213	23	-0.5124949225357085
2213	24	-0.3344661231968544
2213	25	-0.03572301637225195
2214	1	-0.831039292661309
2214	2	-0.6928248339450569
2214	3	-0.6952222465863649
2214	4	-0.5130665049982
2214	5	-0.7194552509200953
2214	6	-0.5367065939981934
2214	7	-0.5509236652442944
2214	8	-0.6732010790213678
2214	9	-10.341935405556107
2214	10	-3.394991624070272
2214	11	-1.3712667358942667
2214	12	-0.9004826270773769
2214	13	-0.6087539315539772
2214	14	-0.725572473967695
2214	15	-0.7864787683959378
2214	16	-0.9781414122459893
2214	17	-0.7925527490201318
2214	18	-1.152815709528923
2214	19	-1.2960735525030032
2214	20	-1.0151352897765875
2214	21	-1.5846043545543296
2214	22	-1.4372917423783989
2214	23	-1.7939989367825113
2214	24	-1.9416580301623796
2214	25	-9.789204583650683
2215	1	-0.3639359432404703
2215	2	-0.008655885345994818
2215	3	0.03475334282495268
2215	4	-0.028222097494740694
2215	5	0.04207035382101054
2215	6	-0.02237313436146593
2215	7	-0.0065745023957504145
2215	8	-0.0014204337893310992
2215	9	0.9189118803014722
2215	10	0.6623439927020058
2215	11	0.653539804300074
2215	12	0.7669089134007407
2215	13	0.6403210578396497
2215	14	0.5303715036349672
2215	15	-0.035709945250680164
2215	16	0.2715260225646432
2215	17	0.14350611972327698
2215	18	0.26037362509026707
2215	19	0.50537987999801
2215	20	0.5836056377546512
2215	21	0.5254629053381947
2215	22	0.3348535724201014
2215	23	0.8182234581580801
2215	24	1.1353754335198118
2215	25	1.1483619238302367
2216	1	-0.6059777896825231
2216	2	-0.5433508982861674
2216	3	-0.586102916342714
2216	4	-0.46669026960042004
2216	5	-1.1953274029719339
2216	6	-1.754588731450223
2216	7	-1.2458137864428416
2216	8	-2.0411413925467343
2216	9	-0.7980556579655982
2216	10	-1.356575853739436
2216	11	-0.8705596135062983
2216	12	-1.8532390543418558
2216	13	-1.6267693656400581
2216	14	-1.7330579423477666
2216	15	-1.3968220967726666
2216	16	-2.308837191470616
2216	17	-2.811410034832857
2216	18	-2.3411089147343773
2216	19	-3.6390127570598843
2216	20	-2.455011013671264
2216	21	-2.6968152960644396
2216	22	-2.4129604228216195
2216	23	-2.0099051816939983
2216	24	-2.2714309547450853
2216	25	-2.3458723220906137
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56344.0
3	3.0
4	742.0
5	7839.0
6	11204.0
7	8915.0
8	6848.0
9	5483.0
10	4696.0
11	4258.0
12	3847.0
13	3586.0
14	3478.0
15	3549.0
16	3773.0
17	3940.0
18	4449.0
19	5729.0
20	7876.0
21	10327.0
22	12532.0
23	14739.0
24	17108.0
25	19253.0
26	21399.0
27	23485.0
28	26164.0
29	30565.0
30	37933.0
31	46339.0
32	57165.0
33	71173.0
34	89006.0
35	112425.0
36	148464.0
37	225402.0
38	494816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84713781125753	19.454830689489466	12.662091386352317	23.035940112900683
2	15.42146077322591	22.517330442070367	39.76365171497381	22.29755706972991
3	17.864927276873786	29.9171762665015	29.853868326963074	22.36402812966164
4	11.09639122548033	17.834249241174664	38.27557993822218	32.79377959512283
5	11.344574480286024	40.42655440479099	34.73232673292012	13.496544382002861
6	27.924197905534037	39.0813264743364	19.491993855568467	13.502481764561086
7	25.68938901502144	33.65661767959451	23.831075307885648	16.8229179974984
8	26.186196442906507	34.74785223153518	21.420718299535793	17.645233026022527
9	27.099797859920606	14.82354349128267	21.243283034133185	36.83337561466354
10	14.48372243204512	31.468344030872743	33.3848003725075	20.66313316457464
11	32.85110881717856	23.83782395563734	24.5902712325898	18.720795994594297
12	22.328982068167882	28.351811877782374	31.390489698045293	17.928716356004447
13	28.192692855803138	22.629677102565843	28.11791778855231	21.059712253078708
14	21.361596696833114	21.882857468672945	31.294776861131517	25.460768973362423
15	22.874151196534807	29.57983099019853	26.736538009757226	20.809479803509436
16	20.907058276615448	28.80603916182576	28.68065950199598	21.606243059562814
17	20.114238425040227	28.341498858986707	30.034199240487713	21.510063475485353
18	19.601071139010635	26.40063515527091	33.48543324481958	20.512860460898878
19	21.08507469197739	26.193357804797486	33.43146847481288	19.290099028412243
20	21.15981209281688	25.549942603304704	33.60405129681632	19.6861940070621
21	22.09456019333558	24.845778355812502	33.1285977000893	19.93106375076262
22	21.16569846063342	25.415127944033912	33.120186753047015	20.298986842285654
23	20.644783374098367	25.454330181183693	33.404851298509094	20.49603514620885
24	20.409206077396956	25.900598591944245	33.56127933916845	20.12891599149035
25	20.542953345833663	25.348655591027207	33.657318866886484	20.451072196252646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1860.0
1	1860.0
2	3092.0
3	4324.0
4	4324.0
5	4324.0
6	15422.5
7	26521.0
8	26521.0
9	26521.0
10	22187.0
11	17853.0
12	17853.0
13	17853.0
14	15488.5
15	13124.0
16	13124.0
17	13124.0
18	22245.5
19	31367.0
20	31367.0
21	31367.0
22	44329.0
23	57291.0
24	57291.0
25	57291.0
26	81321.0
27	105351.0
28	105351.0
29	105351.0
30	119405.5
31	133460.0
32	133460.0
33	133460.0
34	144833.5
35	156207.0
36	156207.0
37	156207.0
38	160338.0
39	164469.0
40	164469.0
41	164469.0
42	171017.0
43	177565.0
44	177565.0
45	177565.0
46	184110.5
47	190656.0
48	190656.0
49	190656.0
50	181462.5
51	172269.0
52	172269.0
53	172269.0
54	152800.5
55	133332.0
56	133332.0
57	133332.0
58	114044.0
59	94756.0
60	94756.0
61	94756.0
62	77612.5
63	60469.0
64	60469.0
65	60469.0
66	46504.0
67	32539.0
68	32539.0
69	32539.0
70	24469.5
71	16400.0
72	16400.0
73	16400.0
74	11693.5
75	6987.0
76	6987.0
77	6987.0
78	5760.0
79	4533.0
80	4533.0
81	4533.0
82	3272.0
83	2011.0
84	2011.0
85	2011.0
86	1435.5
87	860.0
88	860.0
89	860.0
90	737.0
91	614.0
92	614.0
93	614.0
94	324.0
95	34.0
96	34.0
97	34.0
98	18.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02710526938899115
2	0.0011839083181398433
3	0.0
4	0.004424078451996257
5	0.0317785916974379
6	0.009284333652780875
7	0.017509380915647157
8	0.02479976371682409
9	0.03246401230267675
10	0.036825779790560384
11	0.03875741967805171
12	0.006854206052388566
13	0.0025547495286175567
14	0.005670297734248723
15	0.005919541590699216
16	0.007975803406415787
17	0.008723534975767265
18	0.01289836957131303
19	0.016450094525732557
20	0.014456143674128612
21	0.01140290643261007
22	0.010468241970920718
23	0.008038114370528409
24	0.005483364841910853
25	0.10567939513500917
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1604854.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.23201473045496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.90311794885154	32.688838017401515
2	13.354526799075952	11.81395252189966
3	5.020117456628288	6.661497278705894
4	2.4475159303091667	4.330342427298329
5	1.3527074592824055	2.9916488132487826
6	0.8486761131965938	2.2523192604178193
7	0.5642743310995362	1.7471293367648613
8	0.39321610202512763	1.391419233362202
9	0.28425692105710565	1.1315930687488502
>10	1.5569412911653746	13.13190787429419
>50	0.1688442916977765	5.182876297677918
>100	0.09842034597221663	7.799908629160443
>500	0.004544621316242879	1.3256492893949186
>1k	0.0019882718258562595	1.5250965951727162
>5k	1.4201941613258996E-4	0.4968279500461741
>10k+	7.100970806629499E-4	5.528993406405873
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACTTTTTTTTTTTTTTTTTTTTT	22500	1.4019966925340248	No Hit
GTATCAACGCAGAGTACTTTTTTTT	22392	1.3952671084098616	No Hit
TATCAACGCAGAGTACTTTTTTTTT	17752	1.1061442349272892	No Hit
GGTATCAACGCAGAGTACTTTTTTT	14566	0.9076215032644713	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	10806	0.6733322782010077	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	7909	0.4928174151667379	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	3153	0.19646646984710137	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2880	0.17945557664435519	No Hit
GTATAACGCAGAGTACTTTTTTTTT	2804	0.1747199433717958	No Hit
GTGGTATCAACGCAGAGTACTTTTT	2735	0.1704204868480248	No Hit
GTGGTATCAACGCAGAGTACATGGG	1940	0.12088327037848925	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.231096411262333E-5	0.0	0.0	0.0	0.0
2	6.231096411262333E-5	0.0	0.0	0.0	0.0
3	6.231096411262333E-5	0.0	0.0	0.0	0.0
4	6.231096411262333E-5	0.0	0.0	0.0	0.0
5	6.231096411262333E-5	0.0	0.0	0.0	0.0
6	6.231096411262333E-5	0.0	0.0	0.0	0.0
7	1.2462192822524666E-4	0.0	0.0	0.0	0.0
8	1.2462192822524666E-4	0.0	0.0	0.0	0.0
9	1.2462192822524666E-4	0.0	0.0	0.0	0.0
10	1.2462192822524666E-4	0.0	0.0	0.0	0.0
11	1.2462192822524666E-4	0.0	0.0	6.231096411262333E-5	0.0
12	1.2462192822524666E-4	0.0	0.0	6.231096411262333E-5	0.0
13	1.2462192822524666E-4	0.0	0.0	6.231096411262333E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	395	0.0	16.357891	4
CTCGACG	35	0.0021739537	16.281548	13
CGACATG	35	0.0021744063	16.28104	15
TATAACG	380	0.0	15.999397	2
ATAACGC	410	0.0	15.525742	3
TATCACG	140	0.0	14.928008	2
CAACGAG	45	6.7564414E-4	14.779524	1
TCGTCAT	40	0.005288171	14.246354	14
GTATAAC	500	0.0	13.491594	1
TGTACGG	50	0.0014954365	13.304889	5
CTACGGA	50	0.0014982502	13.301571	1
GGTATCA	3545	0.0	13.159523	1
GTATCAC	195	0.0	12.668163	1
ATCACGC	180	0.0	12.6677685	3
TCACGCA	185	0.0	12.326933	4
TCGTTGT	85	3.9109636E-6	12.303241	19
CGGTTTG	140	5.2750693E-11	12.211161	14
GCGCCAC	70	1.093552E-4	12.211161	13
TTACGGA	55	0.003051517	12.099882	19
AAGCGTG	55	0.0030650897	12.092714	7
>>END_MODULE